{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T20:47:06Z","timestamp":1784753226692,"version":"3.55.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100014786","name":"Northern Border University","doi-asserted-by":"publisher","award":["NBU-CRP-2026-2510"],"award-info":[{"award-number":["NBU-CRP-2026-2510"]}],"id":[{"id":"10.13039\/501100014786","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.cryobiol.2025.105574","type":"journal-article","created":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T05:03:37Z","timestamp":1767071017000},"page":"105574","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Nanophytosomal delivery of resveratrol as an effective strategy for enhancing frozen\u2013thawed bovine sperm quality"],"prefix":"10.1016","volume":"122","author":[{"given":"Ali Ali","family":"El-Raghi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amer K.","family":"Mohammed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekramy M.","family":"Elmorsy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud A.E.","family":"Hassan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walaa M.","family":"Essawi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzan Shawky Abuelkasem","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abeer M.E.","family":"Hassan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soha A.","family":"Hassan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cryobiol.2025.105574_bib1","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1017\/S0967199420000271","article-title":"Resveratrol protects boar sperm in vitro via its antioxidant capacity","volume":"28","author":"Sun","year":"2020","journal-title":"Zygote"},{"key":"10.1016\/j.cryobiol.2025.105574_bib2","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.theriogenology.2018.12.017","article-title":"Impact of selenium nano-particles in semen extender on bull sperm quality after cryopreservation","volume":"126","author":"Khalil","year":"2019","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2025.105574_bib3","series-title":"Veterinary Research Forum","first-page":"373","article-title":"Comparison of sperm characteristics and antioxidant and oxidant levels in bull semen frozen with four widely used extenders","author":"Maleki","year":"2023"},{"key":"10.1016\/j.cryobiol.2025.105574_bib4","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S0031-6997(24)01447-9","article-title":"Ligand-binding proteins: their potential for application in systems for controlled delivery and uptake of ligands","volume":"52","author":"De Wolf","year":"2000","journal-title":"Pharmacol. Rev."},{"key":"10.1016\/j.cryobiol.2025.105574_bib5","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.cryobiol.2017.07.005","article-title":"Effect of lecithin nanoliposome or soybean lecithin supplemented by pomegranate extract on post-thaw flow cytometric, microscopic and oxidative parameters in ram semen","volume":"78","author":"Mehdipour","year":"2017","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2025.105574_bib6","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.cryobiol.2020.06.005","article-title":"Resveratrol supplementation and cryopreservation of buck semen","volume":"95","author":"Falchi","year":"2020","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2025.105574_bib7","doi-asserted-by":"crossref","first-page":"2118","DOI":"10.1016\/j.fertnstert.2010.01.058","article-title":"Effects of resveratrol supplementation on cryopreservation medium of human semen","volume":"94","author":"Garcez","year":"2010","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2025.105574_bib8","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1093\/humupd\/dmi047","article-title":"Sperm transport in the female reproductive tract","volume":"12","author":"Suarez","year":"2006","journal-title":"Hum. Reprod. Update"},{"key":"10.1016\/j.cryobiol.2025.105574_bib9","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1016\/S0093-691X(03)00081-5","article-title":"Assessment of fresh and frozen\u2013thawed boar semen using an Annexin-V assay: a new method of evaluating sperm membrane integrity","volume":"60","author":"Pe\u00f1a","year":"2003","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2025.105574_bib10","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.ijpharm.2009.10.011","article-title":"The evidence for solid lipid nanoparticles mediated cell uptake of resveratrol","volume":"390","author":"Teska\u010d","year":"2010","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.cryobiol.2025.105574_bib11","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.colsurfb.2009.03.010","article-title":"Enhanced growth inhibition effect of resveratrol incorporated into biodegradable nanoparticles against glioma cells is mediated by the induction of intracellular reactive oxygen species levels","volume":"72","author":"Shao","year":"2009","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"10.1016\/j.cryobiol.2025.105574_bib12","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.theriogenology.2020.04.034","article-title":"The addition of resveratrol in tris citric acid extender ameliorates post-thaw quality parameters, antioxidant enzymes levels, and fertilizing capability of buffalo (Bubalus bubalis) bull spermatozoa","volume":"152","author":"Ahmed","year":"2020","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2025.105574_bib13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.theriogenology.2016.09.046","article-title":"Resveratrol prevents capacitation-like changes and improves in vitro fertilizing capability of buffalo frozen-thawed sperm","volume":"88","author":"Longobardi","year":"2017","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2025.105574_bib14","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/s00418-020-01871-z","article-title":"Nano-curcumin versus curcumin in amelioration of deltamethrin-induced hippocampal damage","volume":"154","author":"Zaki","year":"2020","journal-title":"Histochem. Cell Biol."},{"key":"10.1016\/j.cryobiol.2025.105574_bib15","unstructured":"A. Khan, S. Mandal, A. Gattani, S. Nath, A.K. Jain, Semen quality correlation with seminal biochemistry and enzymatic profile of Barbari and sirohi bucks under sub tropical environment, Indian J. Anim. Res., 1 7."},{"key":"10.1016\/j.cryobiol.2025.105574_bib16","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/s12944-020-1198-x","article-title":"The effects of resveratrol on lipid profiles and liver enzymes in patients with metabolic syndrome and related disorders: a systematic review and meta-analysis of randomized controlled trials","volume":"19","author":"Akbari","year":"2020","journal-title":"Lipids Health Dis."},{"key":"10.1016\/j.cryobiol.2025.105574_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2021.101290","article-title":"Improving the post-thaw quality of rooster semen using the extender supplemented with resveratrol","volume":"100","author":"Rezaie","year":"2021","journal-title":"Poult. Sci."},{"key":"10.1016\/j.cryobiol.2025.105574_bib18","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.fbio.2016.07.006","article-title":"Nano-phytosome as a potential food-grade delivery system","volume":"15","author":"Ghanbarzadeh","year":"2016","journal-title":"Food Biosci."},{"key":"10.1016\/j.cryobiol.2025.105574_bib19","doi-asserted-by":"crossref","first-page":"6983","DOI":"10.2147\/IJN.S318416","article-title":"Phytosomes as innovative delivery systems for phytochemicals: a comprehensive review of literature","volume":"16","author":"Barani","year":"2021","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.cryobiol.2025.105574_bib20","doi-asserted-by":"crossref","first-page":"38","DOI":"10.33762\/bvetr.2018.143558","article-title":"Evaluation of membrane integrity of bull frozen\u2013thawed sperm using water and hypo-osmotic swelling test","volume":"17","author":"Mohammadi","year":"2018","journal-title":"Basrah J. Vet. Res."},{"key":"10.1016\/j.cryobiol.2025.105574_bib21","doi-asserted-by":"crossref","first-page":"175","DOI":"10.3109\/09537104.2013.801947","article-title":"Platelet counting with the BD AccuriTM C6 flow cytometer","volume":"25","author":"Masters","year":"2014","journal-title":"Platelets"},{"key":"10.1016\/j.cryobiol.2025.105574_bib22","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.anireprosci.2018.12.006","article-title":"Effect of resveratrol-loaded nanostructured lipid carriers supplementation in cryopreservation medium on post-thawed sperm quality and fertility of roosters","volume":"201","author":"Najafi","year":"2019","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2025.105574_bib23","doi-asserted-by":"crossref","first-page":"W270","DOI":"10.1093\/nar\/gkr366","article-title":"SwissDock: a protein\u2013small molecule docking web service based on EADock DSS","volume":"39","author":"Grosdidier","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.cryobiol.2025.105574_bib24","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1016\/S0015-0282(02)04948-8","article-title":"Role of reactive oxygen species in the pathophysiology of human reproduction","volume":"79","author":"Agarwal","year":"2003","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2025.105574_bib25","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S0093-691X(01)00674-4","article-title":"Current status of sperm cryopreservation: why isn't it better?","volume":"57","author":"Medeiros","year":"2002","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2025.105574_bib26","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1556\/004.2015.035","article-title":"Correlation between bull fertility and sperm cell velocity parameters generated by computer-assisted semen analysis","volume":"63","author":"Nagy","year":"2015","journal-title":"Acta Vet. Hung."},{"key":"10.1016\/j.cryobiol.2025.105574_bib27","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.reprotox.2010.11.010","article-title":"Effect of trans-resveratrol on induced oxidative stress in human sperm and in rat germinal cells","volume":"31","author":"Collodel","year":"2011","journal-title":"Reprod. Toxicol."},{"key":"10.1016\/j.cryobiol.2025.105574_bib28","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1002\/9781118833971.ch74","article-title":"Bovine semen quality control in artificial insemination centers","author":"Vincent","year":"2014","journal-title":"Bovine Reprod."},{"key":"10.1016\/j.cryobiol.2025.105574_bib29","doi-asserted-by":"crossref","first-page":"1722","DOI":"10.1016\/j.theriogenology.2011.11.023","article-title":"Effect of antioxidants resveratrol and quercetin on in vitro evaluation of frozen ram sperm","volume":"77","author":"Silva","year":"2012","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2025.105574_bib30","doi-asserted-by":"crossref","first-page":"268","DOI":"10.3389\/fvets.2019.00268","article-title":"Advances in cryopreservation of bull sperm","volume":"6","author":"Ugur","year":"2019","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.cryobiol.2025.105574_bib31","first-page":"265","article-title":"Role of oxidative stress and antioxidant supplementation in male fertility","volume":"21","author":"Beygi","year":"2021","journal-title":"Curr. Mol. Med."},{"key":"10.1016\/j.cryobiol.2025.105574_bib32","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1080\/10408690490468489","article-title":"Antioxidants and prevention of chronic disease","volume":"44","author":"Willcox","year":"2004","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"10.1016\/j.cryobiol.2025.105574_bib33","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1006\/abbi.2000.1973","article-title":"Effects of trans-resveratrol on copper-dependent hydroxyl-radical formation and DNA damage: evidence for hydroxyl-radical scavenging and a novel, glutathione-sparing mechanism of action","volume":"381","author":"Burkitt","year":"2000","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.cryobiol.2025.105574_bib34","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.fertnstert.2015.11.005","article-title":"Association between the seminal plasma proteome and sperm functional traits","volume":"105","author":"Intasqui","year":"2016","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2025.105574_bib35","first-page":"485","article-title":"Effect of resveratrol treatment on apoptosis and apoptotic pathways during boar semen freezing, journal of Zhejiang University","volume":"21","author":"He","year":"2020","journal-title":"Science. B"},{"key":"10.1016\/j.cryobiol.2025.105574_bib36","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1097\/00029330-200807010-00010","article-title":"Resveratrol reestablishes spermatogenesis after testicular injury in rats caused by 2, 5-hexanedione","volume":"121","author":"Jiang","year":"2008","journal-title":"Chin. Med. J."},{"key":"10.1016\/j.cryobiol.2025.105574_bib37","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/j.bbagen.2016.01.017","article-title":"Resveratrol and the mitochondria: from triggering the intrinsic apoptotic pathway to inducing mitochondrial biogenesis, a mechanistic view","volume":"1860","author":"De Oliveira","year":"2016","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"10.1016\/j.cryobiol.2025.105574_bib38","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/s10815-013-0116-9","article-title":"Resveratrol treatment during goat oocytes maturation enhances developmental competence of parthenogenetic and hand-made cloned blastocysts by modulating intracellular glutathione level and embryonic gene expression","volume":"31","author":"Mukherjee","year":"2014","journal-title":"J. Assist. Reprod. Genet."},{"key":"10.1016\/j.cryobiol.2025.105574_bib39","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.theriogenology.2021.01.007","article-title":"Effects of astaxanthin on plasma membrane function and fertility of boar sperm during cryopreservation","volume":"164","author":"Guo","year":"2021","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2025.105574_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/j.theriogenology.2025.117425","article-title":"The beneficial impact of silymarin-loaded chitosan nanoparticles on the quality of frozen-thawed buffalo sperm: Antioxidant and anti-apoptotic effects","volume":"241","author":"El-Raghi","year":"2025","journal-title":"Theriogenology"}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224025003803?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224025003803?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T18:32:04Z","timestamp":1773945124000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0011224025003803"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":40,"alternative-id":["S0011224025003803"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2025.105574","relation":{},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Nanophytosomal delivery of resveratrol as an effective strategy for enhancing frozen\u2013thawed bovine sperm quality","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2025.105574","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Society for Cryobiology. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"105574"}}