{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T09:22:00Z","timestamp":1773998520903,"version":"3.50.1"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82301799"],"award-info":[{"award-number":["82301799"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2025M772346"],"award-info":[{"award-number":["2025M772346"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013280","name":"Major Basic Research Project of the Natural Science Foundation of the Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013280","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012394","name":"Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012394","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010023","name":"Natural Science Research of Jiangsu Higher Education Institutions of China","doi-asserted-by":"publisher","award":["23KJB310033"],"award-info":[{"award-number":["23KJB310033"]}],"id":[{"id":"10.13039\/501100010023","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.cryobiol.2025.105581","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T23:17:57Z","timestamp":1769555877000},"page":"105581","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["An effective method for recovering rat spermatogonial stem cells from frozen rat testis cells"],"prefix":"10.1016","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4323-2560","authenticated-orcid":false,"given":"Fan","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaru","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary R.","family":"Avarbock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eoin C.","family":"Whelan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1408-7656","authenticated-orcid":false,"given":"Ralph L.","family":"Brinster","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.cryobiol.2025.105581_bib1","doi-asserted-by":"crossref","first-page":"2755","DOI":"10.1007\/s10815-023-02945-2","article-title":"Preservation of fertility in female and male prepubertal patients diagnosed with cancer","volume":"40","author":"Albamonte","year":"2023","journal-title":"J. Assist. Reprod. Genet."},{"key":"10.1016\/j.cryobiol.2025.105581_bib2","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1038\/nm0696-693","article-title":"Reconstitution of spermatogenesis from frozen spermatogonial stem cells","volume":"2","author":"Avarbock","year":"1996","journal-title":"Nat Med"},{"key":"10.1016\/j.cryobiol.2025.105581_bib3","doi-asserted-by":"crossref","first-page":"11303","DOI":"10.1073\/pnas.91.24.11303","article-title":"Germline transmission of donor haplotype following spermatogonial transplantation","volume":"91","author":"Brinster","year":"1994","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.cryobiol.2025.105581_bib4","doi-asserted-by":"crossref","DOI":"10.1001\/jamanetworkopen.2024.19771","article-title":"Race and ethnicity, socioeconomic factors, and epigenetic Age acceleration in survivors of childhood cancer","volume":"7","author":"Chen","year":"2024","journal-title":"JAMA Netw. Open"},{"key":"10.1016\/j.cryobiol.2025.105581_bib5","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1038\/381418a0","article-title":"Rat spermatogenesis in mouse testis","volume":"381","author":"Clouthier","year":"1996","journal-title":"Nature"},{"key":"10.1016\/j.cryobiol.2025.105581_bib6","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.cryobiol.2017.04.004","article-title":"Cryoprotectants: a review of the actions and applications of cryoprotective solutes that modulate cell recovery from ultra-low temperatures","volume":"76","author":"Elliott","year":"2017","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2025.105581_bib7","doi-asserted-by":"crossref","first-page":"1314","DOI":"10.1126\/science.aav2914","article-title":"Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring","volume":"363","author":"Fayomi","year":"2019","journal-title":"Science"},{"key":"10.1016\/j.cryobiol.2025.105581_bib8","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1111\/andr.12713","article-title":"Potential use of stem cells for fertility preservation","volume":"8","author":"Gauthier-Fisher","year":"2020","journal-title":"Andrology"},{"key":"10.1016\/j.cryobiol.2025.105581_bib9","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0161372","article-title":"Effect of antioxidants and apoptosis inhibitors on cryopreservation of Murine Germ cells enriched for spermatogonial stem cells","volume":"11","author":"Ha","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.cryobiol.2025.105581_bib10","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/j.stem.2012.07.017","article-title":"Spermatogonial stem cell transplantation into rhesus testes regenerates spermatogenesis producing functional sperm","volume":"11","author":"Hermann","year":"2012","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.cryobiol.2025.105581_bib11","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1530\/rep.1.01125","article-title":"Successful transplantation of bovine testicular cells to heterologous recipients","volume":"132","author":"Herrid","year":"2006","journal-title":"Reproduction"},{"key":"10.1016\/j.cryobiol.2025.105581_bib12","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1002\/mrd.10205","article-title":"Germ cell transplantation in goats","volume":"64","author":"Honaramooz","year":"2003","journal-title":"Mol. Reprod. Dev."},{"key":"10.1016\/j.cryobiol.2025.105581_bib13","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.1093\/jnci\/djab131","article-title":"Annual report to the nation on the status of cancer, part 1: national cancer statistics","volume":"113","author":"Islami","year":"2021","journal-title":"J Natl Cancer Inst"},{"key":"10.1016\/j.cryobiol.2025.105581_bib14","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1111\/j.1365-2605.1995.tb00570.x","article-title":"Male germ cell transplantation in rats: apparent synchronization of spermatogenesis between host and donor seminiferous epithelia","volume":"18","author":"Jiang","year":"1995","journal-title":"Int. J. Androl."},{"key":"10.1016\/j.cryobiol.2025.105581_bib15","doi-asserted-by":"crossref","first-page":"213","DOI":"10.5653\/cerm.2023.06016","article-title":"New strategies for germ cell cryopreservation: cryoinjury modulation","volume":"50","author":"Jung","year":"2023","journal-title":"Clin Exp Reprod Med"},{"key":"10.1016\/j.cryobiol.2025.105581_bib16","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.cryobiol.2015.02.007","article-title":"Cryopreservation of putative pre-pubertal bovine spermatogonial stem cells by slow freezing","volume":"70","author":"Kim","year":"2015","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2025.105581_bib17","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S0091-679X(08)00004-6","article-title":"Culture of rodent spermatogonial stem cells, male germline stem cells of the postnatal animal","volume":"86","author":"Kubota","year":"2008","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.cryobiol.2025.105581_bib18","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.cryobiol.2016.01.004","article-title":"Successful cryopreservation of spermatogonia in critically endangered Manchurian trout (Brachymystax lenok)","volume":"72","author":"Lee","year":"2016","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2025.105581_bib19","article-title":"Cryopreservation in trehalose preserves functional capacity of murine spermatogonial stem cells","volume":"8","author":"Lee","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.cryobiol.2025.105581_bib20","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1095\/biolreprod.113.111195","article-title":"Cryopreservation of mouse spermatogonial stem cells in dimethylsulfoxide and polyethylene glycol","volume":"89","author":"Lee","year":"2013","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2025.105581_bib21","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1016\/j.fertnstert.2013.12.033","article-title":"Effect of sugar molecules on the cryopreservation of mouse spermatogonial stem cells","volume":"101","author":"Lee","year":"2014","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2025.105581_bib22","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1111\/rda.13228","article-title":"Effects of five cryoprotectants on proliferation and differentiation-related gene expression of frozen-thawed bovine calf testicular tissue","volume":"53","author":"Li","year":"2018","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2025.105581_bib23","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1038\/s41570-022-00407-4","article-title":"Chemical approaches to cryopreservation","volume":"6","author":"Murray","year":"2022","journal-title":"Nat. Rev. Chem"},{"key":"10.1016\/j.cryobiol.2025.105581_bib24","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1093\/humupd\/dmw029","article-title":"Cryopreservation of testicular tissue or testicular cell suspensions: a pivotal step in fertility preservation","volume":"22","author":"Onofre","year":"2016","journal-title":"Hum. Reprod. Update"},{"key":"10.1016\/j.cryobiol.2025.105581_bib25","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.rbmo.2019.10.016","article-title":"Testicular tissue cryopreservation is the preferred method to preserve spermatogonial stem cells prior to transplantation","volume":"40","author":"Onofre","year":"2020","journal-title":"Reprod. Biomed. Online"},{"key":"10.1016\/j.cryobiol.2025.105581_bib26","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1095\/biolreprod66.4.944","article-title":"Functional analysis of stem cells in the adult rat testis","volume":"66","author":"Orwig","year":"2002","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2025.105581_bib27","doi-asserted-by":"crossref","first-page":"14302","DOI":"10.1073\/pnas.0506970102","article-title":"Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat","volume":"102","author":"Ryu","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.cryobiol.2025.105581_bib28","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.ydbio.2004.07.004","article-title":"Phenotypic and functional characteristics of spermatogonial stem cells in rats","volume":"274","author":"Ryu","year":"2004","journal-title":"Dev. Biol."},{"key":"10.1016\/j.cryobiol.2025.105581_bib29","first-page":"85","article-title":"The effect of caffeic acid on spermatogonial stem cell-type A cryopreservation","volume":"7","author":"Sayed Mahdi","year":"2018","journal-title":"Rep Biochem Mol Biol"},{"key":"10.1016\/j.cryobiol.2025.105581_bib30","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1007\/BF00496823","article-title":"Immunohistochemical studies of the distribution of a basolateral-membrane protein in intestinal epithelial cells (GZ1-Ag) in rats using monoclonal antibodies","volume":"87","author":"Schiechl","year":"1987","journal-title":"Histochemistry"},{"key":"10.1016\/j.cryobiol.2025.105581_bib31","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1095\/biolreprod.108.075358","article-title":"Identification of glial cell line-derived neurotrophic factor-regulated genes important for spermatogonial stem cell self-renewal in the rat","volume":"81","author":"Schmidt","year":"2009","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2025.105581_bib32","doi-asserted-by":"crossref","first-page":"5504","DOI":"10.1073\/pnas.96.10.5504","article-title":"beta1- and alpha6-integrin are surface markers on mouse spermatogonial stem cells","volume":"96","author":"Shinohara","year":"1999","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.cryobiol.2025.105581_bib33","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1007\/978-1-4939-9524-0_14","article-title":"Isolation, cryopreservation, and transplantation of spermatogonial stem cells","volume":"2005","author":"Sinha","year":"2019","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.cryobiol.2025.105581_bib34","first-page":"193","article-title":"A quantitative study of spermatogonial multiplication and stem cell renewal in the C3H\/101 F1 hybrid mouse","volume":"290","author":"Tegelenbosch","year":"1993","journal-title":"Mutat. Res."},{"key":"10.1016\/j.cryobiol.2025.105581_bib35","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.cryobiol.2019.09.002","article-title":"Trehalose uptake and dehydration effects on the cryoprotection of CHO-K1 cells expressing TRET1","volume":"90","author":"Uchida","year":"2019","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2025.105581_bib36","doi-asserted-by":"crossref","DOI":"10.1177\/0963689721999617","article-title":"Cryopreservation: an overview of principles and cell-specific considerations","volume":"30","author":"Whaley","year":"2021","journal-title":"Cell Transplant."},{"key":"10.1016\/j.cryobiol.2025.105581_bib37","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3001618","article-title":"Reestablishment of spermatogenesis after more than 20 years of cryopreservation of rat spermatogonial stem cells reveals an important impact in differentiation capacity","volume":"20","author":"Whelan","year":"2022","journal-title":"PLoS Biol."},{"key":"10.1016\/j.cryobiol.2025.105581_bib38","doi-asserted-by":"crossref","first-page":"655","DOI":"10.3109\/0284186X.2015.1008648","article-title":"Childhood cancer survivor cohorts in Europe","volume":"54","author":"Winther","year":"2015","journal-title":"Acta Oncol"},{"key":"10.1016\/j.cryobiol.2025.105581_bib39","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1093\/humrep\/des077","article-title":"Fertile offspring derived from mouse spermatogonial stem cells cryopreserved for more than 14 years","volume":"27","author":"Wu","year":"2012","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2025.105581_bib40","doi-asserted-by":"crossref","first-page":"1552","DOI":"10.1111\/rda.13562","article-title":"Trehalose protects testicular tissue of dairy goat upon cryopreservation","volume":"54","author":"Xi","year":"2019","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2025.105581_bib41","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/978-1-0716-3139-3_7","article-title":"Primary cultures of Spermatogonia and Testis cells","volume":"2656","author":"Yang","year":"2023","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.cryobiol.2025.105581_bib42","doi-asserted-by":"crossref","DOI":"10.1016\/j.rbmo.2025.105023","article-title":"Cryopreservation of spermatogonial stem cells: an overview of cryoprotectants","volume":"51","author":"Yang","year":"2025","journal-title":"Reprod. Biomed. Online"},{"key":"10.1016\/j.cryobiol.2025.105581_bib43","article-title":"Ultrasound-Guided Rete Testis approach to sperm aspiration and spermatogonial stem cell transplantation in patients with azoospermia","author":"Zielen","year":"2025","journal-title":"medRxiv"}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224025003876?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224025003876?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T05:40:54Z","timestamp":1773985254000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0011224025003876"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":43,"alternative-id":["S0011224025003876"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2025.105581","relation":{},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An effective method for recovering rat spermatogonial stem cells from frozen rat testis cells","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2025.105581","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Society for Cryobiology. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"105581"}}