{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T13:13:24Z","timestamp":1788614004689,"version":"build-2803163510"},"reference-count":24,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002341","name":"Research Council of Finland","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004022","name":"Jenny and Antti Wihuri Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004022","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003125","name":"Finnish Cultural Foundation","doi-asserted-by":"publisher","award":["00250755"],"award-info":[{"award-number":["00250755"]}],"id":[{"id":"10.13039\/501100003125","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003125","name":"Finnish Cultural Foundation","doi-asserted-by":"publisher","award":["00220283"],"award-info":[{"award-number":["00220283"]}],"id":[{"id":"10.13039\/501100003125","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.cryobiol.2026.105666","type":"journal-article","created":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T15:59:23Z","timestamp":1781452763000},"page":"105666","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Nanofibrillated cellulose hydrogel is a cryoprotectant in conventional and high temperature cryopreservation"],"prefix":"10.1016","volume":"124","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4414-8544","authenticated-orcid":false,"given":"Chris S.","family":"Pridgeon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dahir","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0291-6146","authenticated-orcid":false,"given":"Aleksi","family":"Kr\u00f6ger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eetu","family":"My\u00f6h\u00e4nen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9885-1355","authenticated-orcid":false,"given":"Kerttu","family":"Airavaara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7466-9063","authenticated-orcid":false,"given":"Julia","family":"Monola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikko","family":"Aminoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niklas","family":"Aminoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1583-0379","authenticated-orcid":false,"given":"Riina","family":"Harjum\u00e4ki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjo","family":"Yliperttula","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cryobiol.2026.105666_bib1","series-title":"WO2025062063 - Method for Freezing Mixture Comprising Biological Material, WO\/2025\/062063","author":"Aminoff","year":"2024"},{"key":"10.1016\/j.cryobiol.2026.105666_bib2","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.cryobiol.2019.09.005","article-title":"Effects of nanofibrillated cellulose hydrogels on adipose tissue extract and hepatocellular carcinoma cell spheroids in freeze-drying","volume":"91","author":"Auvinen","year":"2019","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2026.105666_bib3","series-title":"WO2025097235 - Choline Derivatives and Compositions and Uses Thereof for Cryopreservation, PCT\/CA2023\/051510","author":"Ben","year":"2025"},{"key":"10.1016\/j.cryobiol.2026.105666_bib4","doi-asserted-by":"crossref","first-page":"4546","DOI":"10.1039\/D2TB00573E","article-title":"Deep eutectic solvents as cryoprotective agents for mammalian cells","volume":"10","author":"Bryant","year":"2022","journal-title":"J. Mater. Chem. B"},{"key":"10.1016\/j.cryobiol.2026.105666_bib5","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12238","article-title":"Identification of storage conditions stabilizing extracellular vesicles preparations","volume":"11","author":"G\u00f6rgens","year":"2022","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.cryobiol.2026.105666_bib6","doi-asserted-by":"crossref","first-page":"10453","DOI":"10.1021\/jp073113e","article-title":"Modulating the structure and properties of cell membranes: the molecular mechanism of action of dimethyl sulfoxide","volume":"111","author":"Gurtovenko","year":"2007","journal-title":"J. Phys. Chem. B"},{"key":"10.1016\/j.cryobiol.2026.105666_bib7","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1093\/humrep\/14.2.400","article-title":"Vitrification of mouse germinal vesicle oocytes: effect of treatment temperature and egg yolk on chromatin and spindle normality and cumulus integrity","volume":"14","author":"Isachenko","year":"1999","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2026.105666_bib8","series-title":"Infusion-Related Toxicities of DMSO Containing Cryopreserved Blood","author":"Kim","year":"1998"},{"key":"10.1016\/j.cryobiol.2026.105666_bib9","doi-asserted-by":"crossref","first-page":"194","DOI":"10.3390\/micro1020015","article-title":"Comparison of the therapeutic effects of native and anionic nanofibrillar cellulose hydrogels for full-thickness skin wound healing","volume":"1","author":"Koivuniemi","year":"2021","journal-title":"Micro"},{"key":"10.1016\/j.cryobiol.2026.105666_bib10","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.jconrel.2024.02.041","article-title":"Cellulase-assisted platelet-rich plasma release from nanofibrillated cellulose hydrogel enhances wound healing","volume":"368","author":"Koivunotko","year":"2024","journal-title":"J. Contr. Release"},{"key":"10.1016\/j.cryobiol.2026.105666_bib11","doi-asserted-by":"crossref","first-page":"2509","DOI":"10.1093\/humrep\/dem206","article-title":"Effect of using slush nitrogen (SN2) on development of microsurgically manipulated vitrified\/warmed mouse embryos","volume":"22","author":"Lee","year":"2007","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2026.105666_bib12","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1089\/scd.2013.0314","article-title":"The use of nanofibrillar cellulose hydrogel as a flexible three-dimensional model to culture human pluripotent stem cells","volume":"23","author":"Lou","year":"2014","journal-title":"Stem Cell. Dev."},{"key":"10.1016\/j.cryobiol.2026.105666_bib13","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1038\/1831394a0","article-title":"Prevention of freezing damage to living cells by dimethyl sulphoxide","volume":"183","author":"Lovelock","year":"1959","journal-title":"Nature"},{"key":"10.1016\/j.cryobiol.2026.105666_bib14","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1006\/cryo.1993.1006","article-title":"Contributions of cooling and warming rate and developmental stage to the survival of drosophila embryos cooled to -205\u00b0C","volume":"30","author":"Mazur","year":"1993","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2026.105666_bib15","doi-asserted-by":"crossref","first-page":"5530","DOI":"10.3390\/polym14245530","article-title":"Stiffness-controlled hydrogels for 3D cell culture models","volume":"14","author":"Merivaara","year":"2022","journal-title":"Polymers"},{"key":"10.1016\/j.cryobiol.2026.105666_bib16","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/j.jconrel.2021.06.042","article-title":"Preservation of biomaterials and cells by freeze-drying: change of paradigm","volume":"336","author":"Merivaara","year":"2021","journal-title":"J. Contr. Release"},{"key":"10.1016\/j.cryobiol.2026.105666_bib17","first-page":"93","article-title":"Vitrification of human spermatozoa without cryoprotectants","volume":"23","author":"Nawroth","year":"2002","journal-title":"Cryo Lett."},{"key":"10.1016\/j.cryobiol.2026.105666_bib18","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1111\/1541-4337.12202","article-title":"Effects of magnetic fields on freezing: application to biological products","volume":"15","author":"Otero","year":"2016","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"10.1016\/j.cryobiol.2026.105666_bib19","doi-asserted-by":"crossref","DOI":"10.1038\/164666a0","article-title":"Revival of spermatozoa after vitrification and dehydration at low temperatures","volume":"164","author":"Polge","year":"1949","journal-title":"Nature"},{"key":"10.1016\/j.cryobiol.2026.105666_bib20","doi-asserted-by":"crossref","first-page":"78","DOI":"10.21307\/immunohematology-2021-012","article-title":"Effect of cryopreservation on a rare McLeod donor red blood cell concentrate","volume":"37","author":"Turner","year":"2021","journal-title":"Immunohematol"},{"key":"10.1016\/j.cryobiol.2026.105666_bib21","doi-asserted-by":"crossref","first-page":"4641","DOI":"10.1038\/s41598-019-40660-0","article-title":"DMSO induces drastic changes in human cellular processes and epigenetic landscape in vitro","volume":"9","author":"Verheijen","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.cryobiol.2026.105666_bib22","first-page":"13","article-title":"Ultra-rapid freezing and storage of rat embryos in an electric refrigerator at -130 degree C without liquid cryo-agents, with ultra-short exposure in the freezing medium and direct rehydration after thawing","volume":"21","author":"V Isachenko","year":"2000","journal-title":"Cryo Lett."},{"key":"10.1016\/j.cryobiol.2026.105666_bib23","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12404","article-title":"Minimal information for studies of extracellular vesicles (MISEV2023): from basic to advanced approaches","volume":"13","author":"Welsh","year":"2024","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.cryobiol.2026.105666_bib24","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1002\/2211-5463.12193","article-title":"Toxic effects of dimethyl sulfoxide on red blood cells, platelets, and vascular endothelial cells In vitro","volume":"7","author":"Yi","year":"2017","journal-title":"FEBS Open Bio"}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224026000854?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224026000854?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T12:44:53Z","timestamp":1788612293000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0011224026000854"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":24,"alternative-id":["S0011224026000854"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2026.105666","relation":{},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Nanofibrillated cellulose hydrogel is a cryoprotectant in conventional and high temperature cryopreservation","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2026.105666","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc. on behalf of Society for Cryobiology.","name":"copyright","label":"Copyright"}],"article-number":"105666"}}