{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T13:13:39Z","timestamp":1788614019790,"version":"build-2803163510"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["CODE 001"],"award-info":[{"award-number":["CODE 001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.cryobiol.2026.105671","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T16:38:05Z","timestamp":1781627885000},"page":"105671","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Cryopreservation of flushed epididymal spermatozoa outperforms epididymal tail vitrification in Spix's yellow-toothed cavy (Galea spixii)"],"prefix":"10.1016","volume":"124","author":[{"given":"Pedro Augusto Pinheiro","family":"Brito","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9ia Maria","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Santos Costa","family":"Bezerra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilian Leal","family":"Dantas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Euziele Oliveira","family":"de Santana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tayn\u00e1 Moura","family":"Matos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2825-0258","authenticated-orcid":false,"given":"Rom\u00e1rio Parente","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Eduardo Bezerra","family":"Moura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moacir Franco","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6027-1062","authenticated-orcid":false,"given":"Alexandre Rodrigues","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"8","key":"10.1016\/j.cryobiol.2026.105671_bib1","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1007\/s10980-018-0672-6","article-title":"Fragmentation patterns of the Caatinga drylands","volume":"33","author":"Antongiovanni","year":"2018","journal-title":"Landsc. Ecol."},{"issue":"10","key":"10.1016\/j.cryobiol.2026.105671_bib2","doi-asserted-by":"crossref","first-page":"11226","DOI":"10.3168\/jds.2021-20319","article-title":"Comprehensive functional analysis reveals that acrosome integrity and viability are key variables distinguishing artificial insemination bulls of varying fertility","volume":"104","author":"Bernecic","year":"2021","journal-title":"J. Dairy Sci."},{"issue":"3","key":"10.1016\/j.cryobiol.2026.105671_bib3","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1530\/RAF-22-0005","article-title":"Resurrecting biodiversity: advanced assisted reproductive technologies and biobanking","volume":"3","author":"Bolton","year":"2022","journal-title":"Reprod. Fertil."},{"issue":"3\u20134","key":"10.1016\/j.cryobiol.2026.105671_bib4","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.anireprosci.2013.02.015","article-title":"Novel wide-capacity method for vitrification of caprine ovaries: Ovarian Tissue Cryosystem (OTC)","volume":"138","author":"Carvalho","year":"2013","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2026.105671_bib5","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.cryobiol.2015.09.005","article-title":"Comparison among different cryoprotectants for cryopreservation of epididymal sperm from agouti (Dasyprocta leporina)","volume":"71","author":"Castelo","year":"2015","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2026.105671_bib6","article-title":"Galea spixii","author":"Catzeflis","year":"2016","journal-title":"The IUCN Red List of Threatened Species"},{"key":"10.1016\/j.cryobiol.2026.105671_bib7","doi-asserted-by":"crossref","DOI":"10.3389\/fnut.2022.1063510","article-title":"Comprehensive review on the positive and negative effects of various important regulators on male spermatogenesis and fertility","volume":"9","author":"Chao","year":"2023","journal-title":"Front. Nutr."},{"issue":"1","key":"10.1016\/j.cryobiol.2026.105671_bib8","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.theriogenology.2010.12.034","article-title":"Major morphological sperm abnormalities in the bull are related to sperm DNA damage","volume":"76","author":"Enciso","year":"2011","journal-title":"Theriogenology"},{"issue":"8","key":"10.1016\/j.cryobiol.2026.105671_bib9","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.3390\/ani13081360","article-title":"Postmortem collection gametes for the conservation of endangered mammals: a review of the current state-of-the-art","volume":"13","author":"Huijsmans","year":"2023","journal-title":"Animals"},{"issue":"3","key":"10.1016\/j.cryobiol.2026.105671_bib10","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1071\/RD22219","article-title":"Sperm cryopreservation: current status and future developments","volume":"35","author":"Hungerford","year":"2023","journal-title":"Reprod. Fertil. Dev."},{"issue":"3","key":"10.1016\/j.cryobiol.2026.105671_bib11","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1111\/rda.13393","article-title":"Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk extender","volume":"54","author":"Igbokwe","year":"2019","journal-title":"Reprod. Domest. Anim."},{"issue":"15","key":"10.1016\/j.cryobiol.2026.105671_bib12","doi-asserted-by":"crossref","first-page":"5377","DOI":"10.3390\/ijms21155377","article-title":"The role of the epididymis and the contribution of epididymosomes to mammalian reproduction","volume":"21","author":"James","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.cryobiol.2026.105671_bib13","first-page":"163","article-title":"Evolution of the vertebrate epididymis","volume":"53","author":"Jones","year":"1998","journal-title":"J. Reprod. Fertil. Suppl."},{"issue":"2","key":"10.1016\/j.cryobiol.2026.105671_bib14","doi-asserted-by":"crossref","DOI":"10.4172\/2254-609X.100025","article-title":"Effect of cryopreservation on rat epididymis - a histological analysis","volume":"5","author":"Kedam","year":"2016","journal-title":"J. Biomed. Sci."},{"key":"10.1016\/j.cryobiol.2026.105671_bib15","doi-asserted-by":"crossref","DOI":"10.1038\/s41551-025-01369-w","article-title":"Water and ions binding to extracellular matrix drives stress relaxation, aiding MRI detection of swelling-associated pathology","author":"Kollert","year":"2025","journal-title":"Nat. Biomed. Eng."},{"issue":"6","key":"10.1016\/j.cryobiol.2026.105671_bib16","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1017\/S096719942100006X","article-title":"Epididymal tail solid-surface vitrification as an effective method for domestic cat sperm cryobanking","volume":"29","author":"Lima","year":"2021","journal-title":"Zygote"},{"issue":"1","key":"10.1016\/j.cryobiol.2026.105671_bib17","first-page":"77","article-title":"Utilisation of sperm-binding assy combined with computer-assisted sperm analysis to evaluate frozen-thawed bull semen","volume":"41","author":"Losano","year":"2014","journal-title":"Andrology"},{"key":"10.1016\/j.cryobiol.2026.105671_bib18","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.cryobiol.2021.01.016","article-title":"Cryopreservation of Spix's yellow-toothed cavy epididymal sperm using Tris- and coconut water-based extenders supplemented with egg yolk or Aloe vera","volume":"99","author":"Moreira","year":"2021","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2026.105671_bib19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1477-7827-6-39","article-title":"Chorioallantoic placentation in Galea spixii (Rodentia, Caviomorpha, Caviidae)","volume":"6","author":"Oliveira","year":"2008","journal-title":"Reprod. Biol. Endocrinol."},{"issue":"2","key":"10.1016\/j.cryobiol.2026.105671_bib20","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/j.1439-0264.1998.tb00169.x","article-title":"Histological and immunohistochemical Study of the cat epididymis","volume":"27","author":"S\u00e1nchez","year":"1998","journal-title":"Anat. Histol. Embryol."},{"issue":"3","key":"10.1016\/j.cryobiol.2026.105671_bib21","doi-asserted-by":"crossref","first-page":"131","DOI":"10.4322\/jms.089115","article-title":"Development of epididymis and vas deferens in Galea spixii: an ultrastructural view","volume":"33","author":"Santos","year":"2016","journal-title":"J. Morphol. Sci."},{"issue":"8","key":"10.1016\/j.cryobiol.2026.105671_bib22","doi-asserted-by":"crossref","first-page":"12446","DOI":"10.1007\/s10668-020-01177-z","article-title":"Use and traditional management of Galea spixii (Wagler 1831) and Kerodon rupestris (Wied-Neuwied 1820) in the municipalities of Pau dos Ferros, Encanto, and Francisco Dantas (northeastern Brazil)","volume":"23","author":"Santos","year":"2021","journal-title":"Environ. Dev. Sustain."},{"issue":"23","key":"10.1016\/j.cryobiol.2026.105671_bib23","doi-asserted-by":"crossref","first-page":"3271","DOI":"10.3390\/ani12233271","article-title":"Cryopreservation of semen in domestic animals: a review of current challenges, applications, and prospective strategies","volume":"12","author":"Sharafi","year":"2022","journal-title":"Animals"},{"issue":"3","key":"10.1016\/j.cryobiol.2026.105671_bib24","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1111\/azo.12177","article-title":"Characterization of epididymal sperm from Spix's yellow-toothed cavies (Galea spixii Wagler, 1831) recovered by different methods","volume":"98","author":"Silva","year":"2017","journal-title":"Acta Zool."},{"issue":"6","key":"10.1016\/j.cryobiol.2026.105671_bib25","doi-asserted-by":"crossref","first-page":"738","DOI":"10.3390\/ani12060738","article-title":"Cryopreservation of testicular tissue from adult red-rumped agoutis (Dasyprocta leporina Linnaeus, 1758)","volume":"12","author":"Silva","year":"2022","journal-title":"Animals"},{"key":"10.1016\/j.cryobiol.2026.105671_bib26","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.anireprosci.2018.02.009","article-title":"Epididymal sperm from Spix's yellow-toothed cavies sperm successfully cryopreserved in Tris extender with 6% glycerol and 20% egg yolk","volume":"191","author":"Silva","year":"2018","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2026.105671_bib27","first-page":"277","article-title":"Predation of the spix's yellow-toothed cavy, Galea spixii (Rodentia:Caviidae) by the tropical rattlesnake Crotalus durissus cascavella (Serpentes: Viperidae) in the semi-arid region of Brazil","volume":"6","author":"Souza","year":"2013","journal-title":"Herpetol. Notes"},{"key":"10.1016\/j.cryobiol.2026.105671_bib28","first-page":"173","article-title":"Mathematical modeling of protectant transport in tissues","author":"Warner","year":"2020","journal-title":"Methods Mol. Biol."},{"issue":"7","key":"10.1016\/j.cryobiol.2026.105671_bib29","doi-asserted-by":"crossref","first-page":"658","DOI":"10.3390\/vetsci12070658","article-title":"Sperm membrane stability: In-Depth analysis from structural basis to functional regulation","volume":"12","author":"Xue","year":"2025","journal-title":"Vet. Sci."}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224026000908?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224026000908?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T12:47:16Z","timestamp":1788612436000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0011224026000908"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":29,"alternative-id":["S0011224026000908"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2026.105671","relation":{},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cryopreservation of flushed epididymal spermatozoa outperforms epididymal tail vitrification in Spix's yellow-toothed cavy (Galea spixii)","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2026.105671","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc. on behalf of Society for Cryobiology.","name":"copyright","label":"Copyright"}],"article-number":"105671"}}