{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T13:13:22Z","timestamp":1788614002949,"version":"build-2803163510"},"reference-count":63,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["444572\/2024-4"],"award-info":[{"award-number":["444572\/2024-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["20172\/2025-3"],"award-info":[{"award-number":["20172\/2025-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["403157\/2024-2"],"award-info":[{"award-number":["403157\/2024-2"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["305387\/2022-7"],"award-info":[{"award-number":["305387\/2022-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["312072\/2021-0"],"award-info":[{"award-number":["312072\/2021-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["153681\/2025-0"],"award-info":[{"award-number":["153681\/2025-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordination for the improvement of Higher Education Personnel","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.cryobiol.2026.105678","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T10:44:04Z","timestamp":1783939444000},"page":"105678","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Enhancing aquaculture efficiency: Development of a reliable cryopreservation protocol for Leiarius marmoratus"],"prefix":"10.1016","volume":"124","author":[{"given":"Eduardo Thom\u00e9","family":"Nicoleti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4757-5533","authenticated-orcid":false,"given":"R\u00f4mulo Batista","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jhony Lisboa","family":"Benato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0617-8662","authenticated-orcid":false,"given":"Nathalia Dos","family":"Santos Teixeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise Nex","family":"Spica","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larise Caroline Oliveira","family":"Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory Brayan","family":"Valdivia-Guiti\u00e9rrez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8698-0067","authenticated-orcid":false,"given":"Thaiza Rodrigues de","family":"Freitas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karel Gelina","family":"Torres-Lozano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6528-1008","authenticated-orcid":false,"given":"Rodrigo Yutaka Dichoff","family":"Kasai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darci Carlos","family":"Fornari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayme Aparecido","family":"Povh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8735-9638","authenticated-orcid":false,"suffix":"Jr.","given":"Danilo Pedro","family":"Streit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cryobiol.2026.105678_bib1","series-title":"T\u00f3picos Especiais em Piscicultura De \u00c1gua Doce Tropical Intensiva, Editora Tecart","author":"Almeida","year":"2015"},{"key":"10.1016\/j.cryobiol.2026.105678_bib2","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/0011-2240(87)90036-8","article-title":"Mode of interaction of cryoprotectants with membrane phospholipids during freezing","volume":"34","author":"Anchordoguy","year":"1987","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2026.105678_bib3","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/0005-2736(91)90220-3","article-title":"Effect of encapsulated calcium on the surface properties of curved phosphatidylcholine bilayers","volume":"1065","author":"Bakas","year":"1991","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.cryobiol.2026.105678_bib4","series-title":"Methods in Molecular Biology, Hum","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/978-1-4939-2193-5_3","article-title":"Modeling and optimization of cryopreservation","author":"Benson","year":"2015"},{"key":"10.1016\/j.cryobiol.2026.105678_bib5","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1002\/jez.1402610203","article-title":"Some problems related to the assessment of sperm motility in fresh water fish","volume":"261","author":"Billard","year":"1992","journal-title":"J. Exp. Zool."},{"key":"10.1016\/j.cryobiol.2026.105678_bib6","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1590\/1678-4162-10709","article-title":"Ultraestrutura e criopreserva\u00e7\u00e3o de s\u00eamen de jundi\u00e1 amaz\u00f4nico (Leiarius marmoratus) em cativeiro","volume":"72","author":"Borges","year":"2020","journal-title":"Arq. Bras. Med. Vet. Zootec."},{"key":"10.1016\/j.cryobiol.2026.105678_bib7","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1016\/j.theriogenology.2013.06.019","article-title":"Different computer-assisted sperm analysis (CASA) systems highly influence sperm motility parameters","volume":"80","author":"Boryshpolets","year":"2013","journal-title":"Theriogenology"},{"issue":"1","key":"10.1016\/j.cryobiol.2026.105678_bib8","first-page":"11","article-title":"Effect of different cryoprotectants (glycerol, methanol and dimethyl sulfoxide) on post-thaw quality, viability, fertilization ability and DNA damage of cryopreserved nile tilapia (Oreochromis niloticus) spermatozoa","volume":"40","author":"Bozkurt","year":"2019","journal-title":"Cryo Lett."},{"key":"10.1016\/j.cryobiol.2026.105678_bib9","series-title":"Esp\u00e9cies Nativas Para a Piscicultura No Brasil","first-page":"335","article-title":"O cultivo do pintado (Pseudoplatystoma corruscans, Spix; Agassiz, 1829), outras esp\u00e9cies do g\u00eanero Pseudoplatystoma e seus h\u00edbridos","author":"Campos","year":"2010"},{"key":"10.1016\/j.cryobiol.2026.105678_bib10","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1046\/j.1095-8649.2003.00170.x","article-title":"Sperm cryopreservation in the conservation of Brazilian migratory fish","volume":"63","author":"Carolsfeld","year":"2003","journal-title":"J. Fish. Biol."},{"key":"10.1016\/j.cryobiol.2026.105678_bib11","series-title":"Ensayos De Reproducci\u00f3n Y Alevinaje En Yaque Leiarius Marmoratus (Gill, 1870) (Pises:Siluriformes: Pimelodidae) En La Orinoquia Colombiana, Memorias. IV Seminario Internacional De Acuicultura I Congreso Nacional De Investigaciones Acu\u00edcolas, Septiembre","first-page":"10","author":"Castillo","year":"2003"},{"key":"10.1016\/j.cryobiol.2026.105678_bib12","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0005-2736(86)90411-6","article-title":"Prevention of fusion and leakage in freeze-dried liposomes by carbohydrates","volume":"861","author":"Crowe","year":"1986","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.cryobiol.2026.105678_bib13","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1089\/bio.2023.0008","article-title":"Cryopreservation-induced morphological changes in freshwater fish sperm: a systematic review","volume":"22","author":"da Costa","year":"2024","journal-title":"Biopreserv. Bio."},{"key":"10.1016\/j.cryobiol.2026.105678_bib14","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-023-44803-2","article-title":"Cryopreservation effect on DNA methylation profile in rainbow trout spermatozoa","volume":"13","author":"El Kamouh","year":"2023","journal-title":"Sci. Rep."},{"key":"10.1016\/j.cryobiol.2026.105678_bib15","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.aquaculture.2019.03.020","article-title":"Growth curve comparison of native fish cachara (Pseudoplatystoma reticulatum) and cachapinta hybrid (P. reticulatum \u00d7 Pseudoplatystoma corruscans)","volume":"506","author":"Fantini","year":"2019","journal-title":"Aquaculture"},{"key":"10.1016\/j.cryobiol.2026.105678_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.anireprosci.2022.107018","article-title":"Post-thaw dilution of Rhamdia quelen sperm improves the reproductive success","volume":"243","author":"Fran\u00e7a","year":"2022","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2026.105678_bib17","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1111\/raq.12253","article-title":"Fish sperm motility assessment as a tool for aquaculture research: a historical approach","volume":"11","author":"Gallego","year":"2018","journal-title":"Rev. Aquac."},{"key":"10.1016\/j.cryobiol.2026.105678_bib18","first-page":"416","article-title":"Relationship between spermatozoa motility parameters, sperm\/egg ratio, and fertilization and hatching rates in pufferfish (Takifugu niphobles)","author":"Gallego","year":"2013","journal-title":"Aquaculture"},{"key":"10.1016\/j.cryobiol.2026.105678_bib19","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1590\/1519-6984.00313","article-title":"Sperm quality of the Amazon catfish Leiarius marmoratus (Gill, 1870) after cold storage","volume":"74","author":"Galo","year":"2014","journal-title":"Braz. J. Biol."},{"key":"10.1016\/j.cryobiol.2026.105678_bib20","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.cryobiol.2019.02.001","article-title":"Use of trehalose in the sperm cryopreservation of Amazonian catfish Leiarius marmoratus","volume":"87","author":"Gheller","year":"2019","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2026.105678_bib21","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.cryobiol.2008.09.013","article-title":"Biophysics of zebrafish (Danio rerio) sperm","volume":"58","author":"Hagedorn","year":"2009","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2026.105678_bib22","doi-asserted-by":"crossref","first-page":"320","DOI":"10.54680\/fr24510110612","article-title":"Effect of cryoprotectant and concentration on the sperm quality of walking catfish, Clarias batrachus, post-cryopreservation","volume":"45","author":"Handayani","year":"2024","journal-title":"Cryoletters"},{"key":"10.1016\/j.cryobiol.2026.105678_bib23","series-title":"Induced Breeding in Tropical Fish Culture","first-page":"119","article-title":"Preservation of sperm","author":"Harvey","year":"1993"},{"key":"10.1016\/j.cryobiol.2026.105678_bib24","doi-asserted-by":"crossref","DOI":"10.1088\/1755-1315\/441\/1\/012088","article-title":"Optimization of spermatozoa cryopreservation of Albino pangasius catfish: cryoprotectants with various concentrations and different equilibration times","volume":"441","author":"Hasanah","year":"2020","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"10.1016\/j.cryobiol.2026.105678_bib25","doi-asserted-by":"crossref","DOI":"10.1590\/1982-0224-20150139","article-title":"Molecular identification of intergenus crosses involving catfish hybrids: risks for aquaculture production","volume":"14","author":"Hashimoto","year":"2016","journal-title":"Neotrop. Ichthyol."},{"key":"10.1016\/j.cryobiol.2026.105678_bib26","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1111\/j.1753-5131.2012.01067.x","article-title":"Interspecific fish hybrids in Brazil: management of genetic resources for sustainable use","volume":"4","author":"Hashimoto","year":"2012","journal-title":"Rev. Aquac."},{"key":"10.1016\/j.cryobiol.2026.105678_bib27","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/S1472-6483(10)61116-9","article-title":"Methods for cryopreservation of human ovarian tissue","volume":"10","author":"Hovatta","year":"2005","journal-title":"Reprod. Biomed. Online"},{"key":"10.1016\/j.cryobiol.2026.105678_bib28","doi-asserted-by":"crossref","DOI":"10.1111\/and.13166","article-title":"Cryoprotectant-free vitrification of spermatozoa: fish as a model of human","volume":"51","author":"Isachenko","year":"2019","journal-title":"Andrologia"},{"key":"10.1016\/j.cryobiol.2026.105678_bib29","first-page":"1301","article-title":"Effects of different cryoprotectants on sperm quality of Pelteobagrus vachelli","volume":"11","author":"Jiali","year":"2023","journal-title":"J. Fish. Sci. China"},{"key":"10.1016\/j.cryobiol.2026.105678_bib30","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-024-68395-7","article-title":"Cryoprotectant-specific alterations in the proteome of Siberian sturgeon spermatozoa induced by cryopreservation","volume":"14","author":"Kodzik","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.cryobiol.2026.105678_bib31","doi-asserted-by":"crossref","first-page":"43131","DOI":"10.34117\/bjdv7n4-669","article-title":"Rhamdia quelen sperm pre-cryopreservation processing and sperm viability time after thawing","volume":"7","author":"Krause","year":"2021","journal-title":"Braz. J. Dev."},{"key":"10.1016\/j.cryobiol.2026.105678_bib32","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1007\/s10695-014-9993-9","article-title":"Effect of the addition of six antioxidants on sperm motility, membrane integrity and mitochondrial function in red seabream (Pagrus major) sperm cryopreservation","volume":"41","author":"Liu","year":"2015","journal-title":"Fish Physiol. Biochem."},{"key":"10.1016\/j.cryobiol.2026.105678_bib33","series-title":"Checklist of the Freshwater Fishes of South and Central America, EDIPUCRS","first-page":"432","article-title":"Pimelodidae (long-whiskered catfishes)","author":"Lundberg","year":"2003"},{"key":"10.1016\/j.cryobiol.2026.105678_bib34","first-page":"124","article-title":"Criopreserva\u00e7\u00e3o de s\u00eamen de peixes no Brasil: estado da arte e perspectivas futuras, Ci\u00eanc","volume":"22","author":"Maria","year":"2012","journal-title":"Animal"},{"key":"10.1016\/j.cryobiol.2026.105678_bib35","series-title":"Manejo E Sanidade De Peixes em Cultivo","first-page":"47","article-title":"Criopreserva\u00e7\u00e3o de s\u00eamen de peixes no contexto do agroneg\u00f3cio da piscicultura","author":"Maria","year":"2009"},{"key":"10.1016\/j.cryobiol.2026.105678_bib36","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.aquaculture.2006.06.011","article-title":"Extenders and cryoprotectants for cooling and freezing of piracanjuba (Brycon orbignyanus) sperm, an endangered Brazilian teleost fish","volume":"260","author":"Maria","year":"2006","journal-title":"Aquaculture"},{"key":"10.1016\/j.cryobiol.2026.105678_bib37","doi-asserted-by":"crossref","first-page":"3295","DOI":"10.21897\/rmvz.191","article-title":"Effect of freezing and thawing rates on sperm motility in bocachico Prochilodus magdalenae (Pisces, Characiformes)","volume":"18","author":"Mart\u00ednez","year":"2013","journal-title":"Rev. MVZ C\u00f3rdoba"},{"key":"10.1016\/j.cryobiol.2026.105678_bib38","first-page":"19","article-title":"Sperm cryopreservation of freshwater fish bocachico (Prochilodus magdalenae) in DMSO and glucose and its effects on fertilization and hatching efficiency","volume":"9","author":"Mart\u00ednez","year":"2012","journal-title":"Anim. Reprod."},{"key":"10.1016\/j.cryobiol.2026.105678_bib39","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1089\/zeb.2017.1521","article-title":"Changes to extender, cryoprotective medium, and in vitro fertilization improve zebrafish sperm cryopreservation","volume":"15","author":"Matthews","year":"2018","journal-title":"Zebrafish"},{"key":"10.1016\/j.cryobiol.2026.105678_bib40","doi-asserted-by":"crossref","first-page":"2070","DOI":"10.21897\/rmvz.317","article-title":"Evaluaci\u00f3n de tres protocolos hormonales para la inducci\u00f3n de la espermiaci\u00f3n en yaque Leiarius marmoratus","volume":"15","author":"Mira","year":"2010","journal-title":"Rev. MVZ C\u00f3rdoba"},{"key":"10.1016\/j.cryobiol.2026.105678_bib41","series-title":"XIII Congreso Venezolano De Producci\u00f3n E Industria Animal","article-title":"Reproducci\u00f3n inducida del bagre yaque, Leiarius marmoratus, (Gil, 1870) con aplicaci\u00f3n de LHRHa","author":"Mora","year":"2006"},{"key":"10.1016\/j.cryobiol.2026.105678_bib42","doi-asserted-by":"crossref","first-page":"956","DOI":"10.2144\/03355st03","article-title":"Zebrafish sperm cryopreservation with N,N-dimethylacetamide","volume":"35","author":"Morris","year":"2003","journal-title":"Biotechniques"},{"key":"10.1016\/j.cryobiol.2026.105678_bib43","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1590\/S1516-35982007000300002","article-title":"Criopreserva\u00e7\u00e3o do s\u00eamen de curimba (Prochilodus lineatus) mediante adi\u00e7\u00e3o de diferentes diluidores, ativadores e crioprotetores","volume":"36","author":"Murgas","year":"2007","journal-title":"Rev. Bras. Zootec."},{"key":"10.1016\/j.cryobiol.2026.105678_bib44","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.aquaculture.2016.04.021","article-title":"Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality","volume":"472","author":"Mylonas","year":"2017","journal-title":"Aquaculture"},{"key":"10.1016\/j.cryobiol.2026.105678_bib45","first-page":"103","article-title":"Extender composition, osmolality, cryoprotectant and equilibration time effects on fresh sperm motility of two Characiformes fish: piracanjuba (Brycon orbignyanus) and streaked prochilod (Prochilodus lineatus)","volume":"9","author":"Nascimento","year":"2012","journal-title":"Anim. Reprod."},{"key":"10.1016\/j.cryobiol.2026.105678_bib46","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1089\/bio.2022.0091","article-title":"Use of powdered milk in semen cryopreservation protocols for fish: a systematic review","volume":"22","author":"Neyr\u00e3o","year":"2024","journal-title":"Biopreserv. Biobank."},{"key":"10.1016\/j.cryobiol.2026.105678_bib47","series-title":"Associa\u00e7\u00e3o Brasileira Da Piscicultura, Anu\u00e1rio Peixe BR Da Piscicultura 2024: Brasil Produz 887.029 T De Peixes De Cultivo, S\u00e3o Paulo, Brazil: Associa\u00e7\u00e3o Brasileira Da Piscicultura","author":"Peixe-Br","year":"2024"},{"key":"10.1016\/j.cryobiol.2026.105678_bib48","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.anireprosci.2018.09.024","article-title":"Dimethylsulfoxide, methanol and methylglycol in the seminal cryopreservation of suruvi, Steindachneridion scriptum","volume":"200","author":"Pereira","year":"2019","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2026.105678_bib49","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1089\/zeb.2020.1942","article-title":"Oxidative stress and DNA damage of zebrafish sperm at different stages of the cryopreservation process","volume":"18","author":"Rodrigues","year":"2021","journal-title":"Zebrafish"},{"key":"10.1016\/j.cryobiol.2026.105678_bib50","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.cryobiol.2020.10.005","article-title":"Skim milk powder used as a non-permeable cryoprotectant reduces oxidative and DNA damage in cryopreserved zebrafish sperm","volume":"97","author":"Rodrigues","year":"2020","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2026.105678_bib51","doi-asserted-by":"crossref","DOI":"10.1016\/j.anireprosci.2021.106852","article-title":"Successive seminal collections from Leiarius marmoratus during the reproductive period","volume":"234","author":"Spica","year":"2021","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2026.105678_bib52","first-page":"399","article-title":"Germplasm banking and its role in the development of the fish genetic improvement programme in Brazil","volume":"36","author":"Streit","year":"2015","journal-title":"Cryoletters"},{"key":"10.1016\/j.cryobiol.2026.105678_bib53","series-title":"Cryobiology for South American Neotropical Fish Species","first-page":"256","article-title":"Potential use of germplasm banks of neotropical South American species for fish farming in Brazil","author":"Streit","year":"2024"},{"key":"10.1016\/j.cryobiol.2026.105678_bib54","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1111\/j.1439-0426.2010.01533.x","article-title":"Sperm features of captive Atlantic bluefin tuna (Thunnus thynnus)","volume":"26","author":"Suquet","year":"2010","journal-title":"J. Appl. Ichthyol."},{"key":"10.1016\/j.cryobiol.2026.105678_bib55","first-page":"129","article-title":"Use of amides as cryoprotectants in extenders for frozen sperm of tambaqui, Colossoma macropomum","volume":"34","author":"Varela Junior","year":"2012","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2026.105678_bib56","series-title":"Cryopreservation in Aquatic Species","first-page":"387","article-title":"Current status of sperm cryopreservation in siluriform catfishes","author":"Viveiros","year":"2011"},{"key":"10.1016\/j.cryobiol.2026.105678_bib57","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/s10695-008-9240-3","article-title":"Sperm quality and cryopreservation of Brazilian freshwater fish species: a review","volume":"35","author":"Viveiros","year":"2009","journal-title":"Fish Physiol. Biochem."},{"key":"10.1016\/j.cryobiol.2026.105678_bib58","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1111\/j.1365-2109.2010.02761.x","article-title":"Sperm cryopreservation of tiete tetra (Brycon insignis) (Characiformes): effects of cryoprotectants, extenders, thawing temperatures and activating agents on motility features","volume":"42","author":"Viveiros","year":"2010","journal-title":"Aquac. Res."},{"key":"10.1016\/j.cryobiol.2026.105678_bib59","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.theriogenology.2006.10.003","article-title":"Development of a novel CASA system based on open source software for characterization of zebrafish sperm motility parameters","volume":"67","author":"Wilson-Leedy","year":"2007","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2026.105678_bib60","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1111\/rda.13134","article-title":"Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation","volume":"53","author":"Xi","year":"2018","journal-title":"Reprod. Domest. Anim."},{"issue":"4","key":"10.1016\/j.cryobiol.2026.105678_bib61","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1089\/zeb.2019.1849","article-title":"Temporal and concentration effects of methanol on cryopreservation of zebrafish (Danio rerio) sperm","volume":"17","author":"Yang","year":"2020","journal-title":"Zebrafish"},{"key":"10.1016\/j.cryobiol.2026.105678_bib62","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1017\/S1751731114002511","article-title":"Improvement of gamete quality and its short-term storage: an approach for biotechnology in laboratory fish","volume":"9","author":"Yasui","year":"2015","journal-title":"Animal"},{"key":"10.1016\/j.cryobiol.2026.105678_bib63","first-page":"2254","article-title":"Effect of different thawing rates on motility and fertilizing capacity of cryopreserved grass carp (Ctenopharyngodon idella) sperm","volume":"25","author":"Yavas","year":"2011","journal-title":"Biotechnol."}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224026000970?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224026000970?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T12:44:04Z","timestamp":1788612244000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0011224026000970"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":63,"alternative-id":["S0011224026000970"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2026.105678","relation":{},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Enhancing aquaculture efficiency: Development of a reliable cryopreservation protocol for Leiarius marmoratus","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2026.105678","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc. on behalf of Society for Cryobiology.","name":"copyright","label":"Copyright"}],"article-number":"105678"}}