{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T20:56:28Z","timestamp":1790542588849,"version":"4.1.0"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006324","name":"Kiso Seibutsugaku Kenkyujo","doi-asserted-by":"publisher","award":["24NIBB706"],"award-info":[{"award-number":["24NIBB706"]}],"id":[{"id":"10.13039\/501100006324","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.cryobiol.2026.105713","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T22:09:16Z","timestamp":1790287756000},"page":"105713","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Natural mating with sterile triploid males after artificial insemination recapitulates manual sperm mixing to restore the fertility of cryopreserved semen in Bombyx mori"],"prefix":"10.1016","volume":"125","author":[{"given":"Kazunori","family":"Yamamoto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Nishikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kei","family":"Tamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Kimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6832-9146","authenticated-orcid":false,"given":"Toshiaki","family":"Fujimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7380-9770","authenticated-orcid":false,"given":"Masato","family":"Hino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8059-5168","authenticated-orcid":false,"given":"Tsuguru","family":"Fujii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cryobiol.2026.105713_bib1","series-title":"Graduate School of Agriculture","year":"2023"},{"key":"10.1016\/j.cryobiol.2026.105713_bib2","series-title":"Graduate School of Agriculture","year":"2025"},{"key":"10.1016\/j.cryobiol.2026.105713_bib3","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1007\/BF01705783","article-title":"The arrest of embryogenesis at gastrula stage in the diapausing silkworm Bombyx mori is related to the synthesis of protein P61","volume":"199","author":"Coulon-Bublex","year":"1991","journal-title":"Rouxs Arch. Dev. Biol."},{"key":"10.1016\/j.cryobiol.2026.105713_bib4","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1111\/j.1440-6055.1979.tb00868.x","article-title":"Developmental and reproductive biology of Spodoptera litura (F.) (Lepidoptera: Noctuidae)","volume":"18","author":"Etman","year":"1979","journal-title":"J. Aust. Entomol. Soc."},{"key":"10.1016\/j.cryobiol.2026.105713_bib5","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1016\/S0022-1910(97)00044-9","article-title":"Control of the eupyrene-apyrene sperm dimorphism in Lepidoptera","volume":"43","author":"Friedl\u00e4nder","year":"1997","journal-title":"J. Insect Physiol."},{"key":"10.1016\/j.cryobiol.2026.105713_bib6","doi-asserted-by":"crossref","first-page":"594","DOI":"10.2307\/2389920","article-title":"Sperm size and number? Effects of nutritional stress upon eupyrene and apyrene sperm production strategies in the moth Plodia interpunctella (Lepidoptera: Pyralidae)","volume":"8","author":"Gage","year":"1994","journal-title":"Funct. Ecol."},{"key":"10.1016\/j.cryobiol.2026.105713_bib7","first-page":"467","article-title":"Influence of low temperature during egg incubation on spermatogenesis of the silkworm, Bombyx mori. J. Seric","volume":"65","author":"He","year":"1996","journal-title":"Sci. Jpn."},{"key":"10.1016\/j.cryobiol.2026.105713_bib8","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.ibmb.2019.02.002","article-title":"High-quality genome assembly of the silkworm, Bombyx mori","volume":"107","author":"Kawamoto","year":"2019","journal-title":"Insect Biochem. Mol. Biol."},{"key":"10.1016\/j.cryobiol.2026.105713_bib9","first-page":"77","article-title":"Polyploidy and size of serosa nuclei and cells in eggs of the silkworm, Bombyx mori","volume":"48","author":"Kawamura","year":"1979","journal-title":"J. Seric. Sci. Jpn."},{"key":"10.1016\/j.cryobiol.2026.105713_bib10","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/BF00140224","article-title":"The egg size determining gene, Esd, is a unique morphological marker on the W chromosome of Bombyx mori","volume":"76","author":"Kawamura","year":"1988","journal-title":"Genetica"},{"key":"10.1016\/j.cryobiol.2026.105713_bib11","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1046\/j.1440-169X.2002.00641.x","article-title":"In vitro cultivation of spermatocysts to matured sperm in the silkworm Bombyx mori","volume":"44","author":"Kawamura","year":"2002","journal-title":"Dev. Growth Differ."},{"key":"10.1016\/j.cryobiol.2026.105713_bib12","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/dnares\/11.1.27","article-title":"The genome sequence of silkworm, Bombyx mori","volume":"11","author":"Mita","year":"2004","journal-title":"DNA Res."},{"key":"10.1016\/j.cryobiol.2026.105713_bib13","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/BF01330763","article-title":"X-ray-induced recombination during oogenesis in the silkworm (Bombyx mori L.)","volume":"13","author":"Murakami","year":"1976","journal-title":"Radiat. Environ. Biophys."},{"key":"10.1016\/j.cryobiol.2026.105713_bib14","first-page":"129","article-title":"Studies on the reproductive system of the male of Bombyx mori. \u2161. Post-testicular organs and post-testicular behavior of the spermatozoa","volume":"40","author":"Omura","year":"1938","journal-title":"J. Fac. Agr. Hokkaido Imp. Univ."},{"key":"10.1016\/j.cryobiol.2026.105713_bib15","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1266\/jjg.15.29","article-title":"Selective fertilization in Bombyx mori","volume":"15","author":"Omura","year":"1939","journal-title":"Jpn. J. Genet."},{"key":"10.1016\/j.cryobiol.2026.105713_bib16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/jmor.1051930102","article-title":"The spermatophore and its structural changes with time in the bursa copulatrix of the silkworm, Bombyx mori","volume":"193","author":"Osanai","year":"1987","journal-title":"J. Morphol."},{"key":"10.1016\/j.cryobiol.2026.105713_bib17","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1038\/hdy.2015.74","article-title":"Positional cloning of a Bombyx pink-eyed white egg locus reveals the major role of cardinal in ommochrome synthesis","volume":"116","author":"Osanai-Futahashi","year":"2016","journal-title":"Heredity"},{"key":"10.1016\/j.cryobiol.2026.105713_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.ibmb.2022.103728","article-title":"The red egg gene as a novel effective egg color marker for silkworm transgenesis","volume":"143","author":"Osanai-Futahashi","year":"2022","journal-title":"Insect Biochem. Mol. Biol."},{"key":"10.1016\/j.cryobiol.2026.105713_bib19","series-title":"A Language and Environment for Statistical Computing","year":"2023"},{"key":"10.1016\/j.cryobiol.2026.105713_bib20","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1017\/S0967199402002046","article-title":"Double copulation of a female with sterile diploid and polyploid males recovers fertility in Bombyx mori","volume":"10","author":"Sahara","year":"2002","journal-title":"Zygote"},{"key":"10.1016\/j.cryobiol.2026.105713_bib21","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1002\/jez.a.10250","article-title":"Application of artificial insemination technique to eupyrene and\/or apyrene sperm in Bombyx mori","volume":"297","author":"Sahara","year":"2003","journal-title":"J. Exp. Zool. Comp. Exp. Biol."},{"key":"10.1016\/j.cryobiol.2026.105713_bib22","first-page":"380","article-title":"Preferential pairing of sex chromosomes in homotype and heterotype 94 tetraploid silkworm females of sex-limited normal pattern strain","volume":"64","author":"Sahara","year":"1995","journal-title":"HOMARE. J. Seric. Sci. Jpn"},{"key":"10.1016\/j.cryobiol.2026.105713_bib23","first-page":"3","article-title":"A history of chromosome identification in Bombyx mori. Chromosom","volume":"19","author":"Sahara","year":"2016","journal-title":"Sci"},{"key":"10.1016\/j.cryobiol.2026.105713_bib24","first-page":"39","article-title":"Double-copulated introduction of ejaculate with dominant larval phenotype to maintain Bombyx mori mutant with dysfunctional apyrene sperm","volume":"89","author":"Sakai","year":"2020","journal-title":"J. Insect Biotechnol. Sericol."},{"key":"10.1016\/j.cryobiol.2026.105713_bib25","doi-asserted-by":"crossref","first-page":"10412","DOI":"10.1073\/pnas.1820101116","article-title":"Dimorphic sperm formation by Sex-lethal","volume":"116","author":"Sakai","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cryobiol.2026.105713_bib26","first-page":"176","article-title":"Male sterility in the triploid silkworm","volume":"11","author":"Sugai","year":"1976","journal-title":"Bombyx mori L. (Lepidoptera: Bombycidae). Appl. Entomol. Zool."},{"key":"10.1016\/j.cryobiol.2026.105713_bib27","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/0022-1910(95)00104-2","article-title":"Sperm precedence and sperm movement under different copulation intervals in the silkworm, Bombyx mori","volume":"42","author":"Suzuki","year":"1996","journal-title":"J. Insect Physiol."},{"key":"10.1016\/j.cryobiol.2026.105713_bib28","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/S0022-1910(98)00148-6","article-title":"Artificial insemination using trypsin-treated sperm in the silkworm, Bombyx mori","volume":"45","author":"Takemura","year":"1999","journal-title":"J. Insect Physiol."},{"key":"10.1016\/j.cryobiol.2026.105713_bib29","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/S0022-1910(99)00135-3","article-title":"Artificial insemination using cryopreserved sperm in the silkworm, Bombyx mori","volume":"46","author":"Takemura","year":"2000","journal-title":"J. Insect Physiol."},{"key":"10.1016\/j.cryobiol.2026.105713_bib30","first-page":"456","article-title":"Development of new method for artificial insemination of the silkworm, Bombyx mori","volume":"65","author":"Takemura","year":"1996","journal-title":"J. Seric. Sci. Jpn."},{"key":"10.1016\/j.cryobiol.2026.105713_bib31","first-page":"21","article-title":"A role in insemination of spermatophore of the silkworm male moth, their testes were removed at larval stage","volume":"59","author":"Takemura","year":"2011","journal-title":"Journal of Dainippon Silk Foundation"},{"key":"10.1016\/j.cryobiol.2026.105713_bib32","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1016\/j.jinsphys.2006.06.010","article-title":"Apyrene sperm from the triploid donors restore fecundity of cryopreserved semen in Bombyx mori","volume":"52","author":"Takemura","year":"2006","journal-title":"J. Insect Physiol."},{"key":"10.1016\/j.cryobiol.2026.105713_bib33","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1303\/aez.2009.413","article-title":"Fertility restoration of cryopreserved semen by hyper ratio of apyrene sperm in Bombyx mori","volume":"44","author":"Takemura","year":"2009","journal-title":"Appl. Entomol. Zool."},{"key":"10.1016\/j.cryobiol.2026.105713_bib34","first-page":"517","article-title":"Eupyrene and apyrene spermatozoa of the triploid male of the silkworm, Bombyx mori L","volume":"45","author":"Takizawa","year":"1976","journal-title":"J. Seric. Sci. Jpn."},{"key":"10.1016\/j.cryobiol.2026.105713_bib35","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1023\/A:1014638723413","article-title":"The effect of W chromosome origin on sex-chromosome pairing in ZZWW tetraploid females of the domesticated silkworm, Bombyx mori, and the congenic wild silkworm, Bombyx mandarina","volume":"114","author":"Tanaka","year":"2002","journal-title":"Genetica"},{"key":"10.1016\/j.cryobiol.2026.105713_bib36","first-page":"286","article-title":"Experimental induction of androgenesis, gynogenesis and polyploidy in Bombyx mori by treatment with CO2 gas","volume":"36","author":"Tazima","year":"1967","journal-title":"J. Seric. Sci. Jpn."},{"key":"10.1016\/j.cryobiol.2026.105713_bib37","doi-asserted-by":"crossref","first-page":"52","DOI":"10.2144\/00291bm09","article-title":"Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and tris (HotSHOT)","volume":"29","author":"Truett","year":"2000","journal-title":"Biotechniques"},{"key":"10.1016\/j.cryobiol.2026.105713_bib38","first-page":"370","article-title":"Embryonic death in the eggs oviposited by a triploid female mated with a normal moth in Bombyx mori","volume":"61","author":"Yokoyama","year":"1992","journal-title":"J. Seric. Sci. Jpn."},{"key":"10.1016\/j.cryobiol.2026.105713_bib39","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.jinsphys.2017.07.013","article-title":"Pigmentary analysis of eggs of the silkworm Bombyx mori","volume":"101","author":"Zhang","year":"2017","journal-title":"J. Insect Physiol."}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S001122402600132X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S001122402600132X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T20:01:52Z","timestamp":1790539312000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S001122402600132X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":39,"alternative-id":["S001122402600132X"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2026.105713","relation":{},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Natural mating with sterile triploid males after artificial insemination recapitulates manual sperm mixing to restore the fertility of cryopreserved semen in Bombyx mori","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2026.105713","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc. on behalf of Society for Cryobiology.","name":"copyright","label":"Copyright"}],"article-number":"105713"}}