{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:38:42Z","timestamp":1792615122057,"version":"4.5.0"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005771","name":"Dokuz Eyl\u00fcl University","doi-asserted-by":"publisher","award":["THD-2023-2793"],"award-info":[{"award-number":["THD-2023-2793"]}],"id":[{"id":"10.13039\/501100005771","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.cryobiol.2026.105720","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T19:20:37Z","timestamp":1792524037000},"page":"105720","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Modulation of apoptotic genes and microRNAs by Shilajit in cryopreserved rooster sperm"],"prefix":"10.1016","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7722-5772","authenticated-orcid":false,"given":"Zeynep","family":"Semen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0830-1644","authenticated-orcid":false,"given":"Mustafa Yi\u011fit","family":"Nizam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murat","family":"Sel\u00e7uk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.cryobiol.2026.105720_bib1","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1016\/j.fertnstert.2012.11.054","article-title":"Altered microRNA expression profiles of human spermatozoa in patients with different spermatogenic impairments","volume":"99","author":"Abu-Halima","year":"2013","journal-title":"Fertil. Steril."},{"issue":"5","key":"10.1016\/j.cryobiol.2026.105720_bib2","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1002\/ptr.2100","article-title":"Shilajit: a review","volume":"21","author":"Agarwal","year":"2007","journal-title":"Phytother Res. : PTR"},{"issue":"1\u20132","key":"10.1016\/j.cryobiol.2026.105720_bib3","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.mce.2005.12.026","article-title":"Oxidative stress, sperm survival and fertility control","volume":"250","author":"Aitken","year":"2006","journal-title":"Mol. Cell. Endocrinol."},{"issue":"2","key":"10.1016\/j.cryobiol.2026.105720_bib4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1071\/RD15325","article-title":"Causes and consequences of oxidative stress in spermatozoa","volume":"28","author":"Aitken","year":"2016","journal-title":"Reprod. Fertil. Dev."},{"issue":"3","key":"10.1016\/j.cryobiol.2026.105720_bib5","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.cryobiol.2015.03.001","article-title":"The effects of different levels of catalase and superoxide dismutase in modified Beltsville extender on rooster post-thawed sperm quality","volume":"70","author":"Amini","year":"2015","journal-title":"Cryobiology"},{"issue":"2","key":"10.1016\/j.cryobiol.2026.105720_bib6","doi-asserted-by":"crossref","first-page":"52","DOI":"10.3184\/175815511X13069413108523","article-title":"Freezing avian semen","volume":"4","author":"Blesbois","year":"2011","journal-title":"Avian Biol. Res."},{"issue":"4","key":"10.1016\/j.cryobiol.2026.105720_bib7","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1261\/rna.1963810","article-title":"Role of miR-34c microRNA in the late steps of spermatogenesis","volume":"16","author":"Bouhallier","year":"2010","journal-title":"RNA (New York, N.Y.)"},{"key":"10.1016\/j.cryobiol.2026.105720_bib8","article-title":"Shilajit: a natural phytocomplex with potential procognitive activity","volume":"2012","author":"Carrasco-Gallardo","year":"2012","journal-title":"Int. J. Alzheimers Dis."},{"issue":"4","key":"10.1016\/j.cryobiol.2026.105720_bib9","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/j.theriogenology.2006.02.022","article-title":"Effect of docosahexaenoic acid and \u03b1-tocopherol enrichment in chicken sperm on semen quality, sperm lipid enrichment in chicken sperm on composition and susceptibility to peroxidation","volume":"66","author":"Cerolini","year":"2006","journal-title":"Theriogenology,"},{"issue":"4","key":"10.1016\/j.cryobiol.2026.105720_bib10","doi-asserted-by":"crossref","first-page":"802","DOI":"10.3390\/ijms20040802","article-title":"Exploration of miRNA and mRNA profiles in fresh and frozen-thawed boar sperm by transcriptome and small RNA sequencing","volume":"20","author":"Dai","year":"2019","journal-title":"Int. J. Mol. Sci."},{"issue":"4","key":"10.1016\/j.cryobiol.2026.105720_bib11","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1071\/RD9950857","article-title":"Derivation and reliability of kinematic measures of sperm motion","volume":"7","author":"Davis","year":"1995","journal-title":"Reprod. Fertil. Dev."},{"issue":"2","key":"10.1016\/j.cryobiol.2026.105720_bib12","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.cmet.2006.01.005","article-title":"miR-122 regulation of lipid metabolism revealed by in vivo antisense targeting","volume":"3","author":"Esau","year":"2006","journal-title":"Cell Metab."},{"key":"10.1016\/j.cryobiol.2026.105720_bib13","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.cryobiol.2016.10.009","article-title":"L-Carnitine in rooster semen cryopreservation: flow cytometric, biochemical and motion findings for frozen-thawed sperm","volume":"74","author":"Fattah","year":"2017","journal-title":"Cryobiology"},{"issue":"7","key":"10.1016\/j.cryobiol.2026.105720_bib14","first-page":"1173","article-title":"Rapid sperm capture: high-throughput flagellar waveform analysis","volume":"34","author":"Gallagher","year":"2019","journal-title":"Hum. Reprod(Oxford, England)"},{"issue":"11","key":"10.1016\/j.cryobiol.2026.105720_bib15","doi-asserted-by":"crossref","first-page":"3846","DOI":"10.3390\/ijms21113846","article-title":"MiRNAs expression profiling of bovine (Bos taurus) testes and effect of bta-miR-146b on proliferation and apoptosis in bovine Male germline stem cells","volume":"21","author":"Gao","year":"2020","journal-title":"Int. J. Mol. Sci."},{"issue":"12","key":"10.1016\/j.cryobiol.2026.105720_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2023.103135","article-title":"Identification of key genes affecting sperm motility in chicken based on whole-transcriptome sequencing","volume":"102","author":"Guo","year":"2023","journal-title":"Poult. Sci."},{"issue":"3","key":"10.1016\/j.cryobiol.2026.105720_bib17","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1242\/jeb.29.3.445","article-title":"The morphology of bull spermatozoa","volume":"29","author":"Hancock","year":"1952","journal-title":"J. Exp. Biol."},{"issue":"Pt 20","key":"10.1016\/j.cryobiol.2026.105720_bib18","doi-asserted-by":"crossref","first-page":"4077","DOI":"10.1242\/jcs.00739","article-title":"Apoptosis - the p53 network","volume":"116","author":"Haupt","year":"2003","journal-title":"J. Cell Sci."},{"issue":"7148","key":"10.1016\/j.cryobiol.2026.105720_bib19","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1038\/nature05939","article-title":"A microRNA component of the p53 tumour suppressor network","volume":"447","author":"He","year":"2007","journal-title":"Nature"},{"issue":"11","key":"10.1016\/j.cryobiol.2026.105720_bib20","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1101\/gad.1777409","article-title":"The PTEN-regulating microRNA miR-26a is amplified in high-grade glioma and facilitates gliomagenesis in vivo","volume":"23","author":"Huse","year":"2009","journal-title":"Gene Dev."},{"issue":"6","key":"10.1016\/j.cryobiol.2026.105720_bib21","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1093\/humupd\/dmt031","article-title":"The presence, role and clinical use of spermatozoal RNAs","volume":"19","author":"Jodar","year":"2013","journal-title":"Hum. Reprod. Update"},{"issue":"10","key":"10.1016\/j.cryobiol.2026.105720_bib22","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1111\/and.12200","article-title":"p53 and mitogen\u2010activated protein kinase pathway protein profiles in fresh and frozen spermatozoa","volume":"46","author":"Karabulut","year":"2014","journal-title":"Andrologia"},{"issue":"1","key":"10.1016\/j.cryobiol.2026.105720_bib23","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/s12970-019-0270-2","article-title":"The effects of Shilajit supplementation on fatigue-induced decreases in muscular strength and serum hydroxyproline levels","volume":"16","author":"Keller","year":"2019","journal-title":"J. Int. Soc. Sports Nutr."},{"issue":"2","key":"10.1016\/j.cryobiol.2026.105720_bib24","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1093\/ps\/85.2.232","article-title":"Avian semen cryopreservation: what are the biological challenges?","volume":"85","author":"Long","year":"2006","journal-title":"Poult. Sci."},{"issue":"10","key":"10.1016\/j.cryobiol.2026.105720_bib25","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0241105","article-title":"Effect of crocin and naringenin supplementation in cryopreservation medium on post-thaw rooster sperm quality and expression of apoptosis associated genes","volume":"15","author":"Mehdipour","year":"2020","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.cryobiol.2026.105720_bib26","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.cryobiol.2015.11.005","article-title":"Comparative evaluation of Nabi and Beltsville extenders for cryopreservation of rooster semen","volume":"72","author":"Nabi","year":"2016","journal-title":"Cryobiology"},{"issue":"1","key":"10.1016\/j.cryobiol.2026.105720_bib27","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.psj.2020.10.007","article-title":"Does alpha-lipoic acid-loaded nanostructured lipid carriers improve post-thawed sperm quality and ameliorate apoptosis-related genes of rooster sperm?","volume":"100","author":"Najafi","year":"2021","journal-title":"Poult. Sci."},{"key":"10.1016\/j.cryobiol.2026.105720_bib28","doi-asserted-by":"crossref","first-page":"4527","DOI":"10.1038\/s41598-024-55057-x","article-title":"Apigenin supplementation substantially improves rooster sperm freezability and post-thaw function","volume":"14","author":"Najafi","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.cryobiol.2026.105720_bib29","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2025.1534638","article-title":"Which is more effective in thawing frozen rooster sperm: varying temperature or duration?","volume":"12","author":"Nizam","year":"2025","journal-title":"Front. Vet. Sci."},{"issue":"2","key":"10.1016\/j.cryobiol.2026.105720_bib30","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1038\/nri2708","article-title":"Physiological and pathological roles for microRNAs in the immune system","volume":"10","author":"O'Connell","year":"2010","journal-title":"Nat. Rev. Immunol."},{"issue":"3\u20134","key":"10.1016\/j.cryobiol.2026.105720_bib31","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.anireprosci.2012.07.007","article-title":"Lipid peroxidation and antioxidant enzymes activity in avian semen","volume":"134","author":"Partyka","year":"2012","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2026.105720_bib32","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.theriogenology.2021.07.022","article-title":"Spermatozoa and seminal plasma small extracellular vesicles miRNAs as biomarkers of boar semen cryotolerance","volume":"174","author":"Pedrosa","year":"2021","journal-title":"Theriogenology"},{"issue":"8","key":"10.1016\/j.cryobiol.2026.105720_bib33","doi-asserted-by":"crossref","first-page":"77","DOI":"10.14715\/cmb\/2017.63.8.17","article-title":"New insights into the expression profile of MicroRNA-34c and P53 in infertile men spermatozoa and testicular tissue","volume":"63","author":"Rahbar","year":"2017","journal-title":"Cell. Mol. Biol."},{"issue":"6","key":"10.1016\/j.cryobiol.2026.105720_bib34","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0067614","article-title":"Cryopreservation causes genetic and epigenetic changes in zebrafish genital ridges","volume":"8","author":"Riesco","year":"2013","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.cryobiol.2026.105720_bib35","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/s00441-023-03764-8","article-title":"Cryostress induces fragmentation and alters the abundance of sperm transcripts associated with fertilizing competence and reproductive processes in buffalo","volume":"393","author":"Selvaraju","year":"2023","journal-title":"Cell Tissue Res."},{"issue":"1","key":"10.1016\/j.cryobiol.2026.105720_bib36","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.jep.2012.06.002","article-title":"Shilajit attenuates behavioral symptoms of chronic fatigue syndrome by modulating the hypothalamic-pituitary-adrenal axis and mitochondrial bioenergetics in rats","volume":"143","author":"Surapaneni","year":"2012","journal-title":"J. Ethnopharmacol."},{"issue":"33","key":"10.1016\/j.cryobiol.2026.105720_bib37","doi-asserted-by":"crossref","first-page":"12481","DOI":"10.1073\/pnas.0605298103","article-title":"NF-kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses","volume":"103","author":"Taganov","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U. S. A"},{"issue":"1","key":"10.1016\/j.cryobiol.2026.105720_bib38","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1111\/imr.12057","article-title":"miR-155: an ancient regulator of the immune system","volume":"253","author":"Vigorito","year":"2013","journal-title":"Immunol. Rev."},{"issue":"3","key":"10.1016\/j.cryobiol.2026.105720_bib39","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/S0015-0282(03)00756-8","article-title":"Oxidative stress is associated with increased apoptosis leading to spermatozoa DNA damage in patients with male factor infertility","volume":"80","author":"Wang","year":"2003","journal-title":"Fertil. Steril."},{"issue":"2","key":"10.1016\/j.cryobiol.2026.105720_bib40","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1530\/jrf.0.0660457","article-title":"Maintenance of ATP concentrations in and of fertilizing ability of fowl and Turkey spermatozoa in vitro","volume":"66","author":"Wishart","year":"1982","journal-title":"J. Reprod. Fertil."},{"issue":"36","key":"10.1016\/j.cryobiol.2026.105720_bib41","doi-asserted-by":"crossref","first-page":"13421","DOI":"10.1073\/pnas.0801613105","article-title":"miR-34a repression of SIRT1 regulates apoptosis","volume":"105","author":"Yamakuchi","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U. S. A"},{"issue":"1","key":"10.1016\/j.cryobiol.2026.105720_bib42","doi-asserted-by":"crossref","first-page":"8865","DOI":"10.1038\/s41598-019-45087-1","article-title":"Demethylation and microRNA differential expression regulate plasma-induced improvement of chicken sperm quality","volume":"9","author":"Zhang","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.cryobiol.2026.105720_bib43","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.cryobiol.2017.04.013","article-title":"Cryopreservation of boar sperm induces differential microRNAs expression","volume":"76","author":"Zhang","year":"2017","journal-title":"Cryobiology"}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224026001392?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224026001392?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:43:10Z","timestamp":1792611790000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0011224026001392"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":43,"alternative-id":["S0011224026001392"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2026.105720","relation":{},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Modulation of apoptotic genes and microRNAs by Shilajit in cryopreserved rooster sperm","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2026.105720","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc. on behalf of Society for Cryobiology.","name":"copyright","label":"Copyright"}],"article-number":"105720"}}