{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T13:08:13Z","timestamp":1779023293366,"version":"3.51.4"},"reference-count":88,"publisher":"American Association for the Advancement of Science (AAAS)","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T00:00:00Z","timestamp":1605484800000},"content-version":"vor","delay-in-days":320,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GU1100\/16"],"award-info":[{"award-number":["GU1100\/16"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["csbj.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Comput Struct Biotechnol J"],"published-print":{"date-parts":[[2020,1]]},"DOI":"10.1016\/j.csbj.2020.11.024","type":"journal-article","created":{"date-parts":[[2020,11,21]],"date-time":"2020-11-21T02:18:43Z","timestamp":1605925123000},"page":"3861-3876","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"title":["The protozoan parasite\n                    <i>Toxoplasma gondii<\/i>\n                    encodes a gamut of phosphodiesterases during its lytic cycle in human cells"],"prefix":"10.34133","volume":"18","author":[{"given":"Kim Chi","family":"Vo","sequence":"first","affiliation":[{"name":"Department of Molecular Parasitology, Institute of Biology, Faculty of Life Sciences, Humboldt University, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d6zlem","family":"G\u00fcnay-Esiyok","sequence":"additional","affiliation":[{"name":"Department of Molecular Parasitology, Institute of Biology, Faculty of Life Sciences, Humboldt University, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"Liem","sequence":"additional","affiliation":[{"name":"Experimental Biophysics, Institute of Biology, Faculty of Life Sciences, Humboldt University, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nishith","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Molecular Parasitology, Institute of Biology, Faculty of Life Sciences, Humboldt University, Berlin, Germany"},{"name":"Department of Biological Sciences, Birla Institute of Technology and Science Pilani (BITS-P), Hyderabad, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","published-online":{"date-parts":[[2020,12,5]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm911"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.170213"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-020518-120235"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2020.01.009"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/15216540400013937"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000008"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004520"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000746"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000264"},{"key":"e_1_3_5_11_2","doi-asserted-by":"crossref","DOI":"10.1038\/srep34479","article-title":"Hierarchical phosphorylation of apical membrane antigen 1 is required for efficient red blood cell invasion by malaria parasites","volume":"6","author":"Prinz B.","year":"2016","unstructured":"Prinz B., Harvey K.L., Wilcke L., Ruch U., Engelberg K., Biller L., Lucet I., Erkelenz S., Heincke D., Spielmann T., Doerig C., Kunick C., Crabb B.S., Gilson P.R. and Gilberger T.W.: Hierarchical phosphorylation of apical membrane antigen 1 is required for efficient red blood cell invasion by malaria parasites. Sci Rep 2016; 6.","journal-title":"Sci Rep"},{"key":"e_1_3_5_12_2","first-page":"e01972","article-title":"Protein kinase A is essential for invasion of Plasmodium falciparum into human erythrocytes","volume":"10","author":"Wilde M.-L.","year":"2019","unstructured":"Wilde M.-L., Triglia T., Marapana D., Thompson J.K., Kouzmitchev A.A., Bullen H.E. et al: Protein kinase A is essential for invasion of Plasmodium falciparum into human erythrocytes. mBio 2019; 10: e01972.","journal-title":"mBio"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.10.015"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201796794"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.08.014"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.465583"},{"key":"e_1_3_5_17_2","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1128\/IAI.69.1.148-153.2001","article-title":"Cyclic nucleotide signaling in Toxoplasma gondii bradyzoite differentiation","volume":"69","author":"Petri W.A.","year":"2001","unstructured":"Petri W.A., Kirkman L.A., Weiss L.M. and Kim K.: Cyclic nucleotide signaling in Toxoplasma gondii bradyzoite differentiation. Infect Immun 2001; 69: 148.","journal-title":"Infect Immun"},{"key":"e_1_3_5_18_2","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00755-16","article-title":"Toxoplasma gondii cyclic AMP-dependent protein kinase subunit 3 Is involved in the switch from tachyzoite to bradyzoite development","volume":"7","author":"Sugi T.","year":"2016","unstructured":"Sugi T., Ma Y.F., Tomita T., Murakoshi F., Eaton M.S., Yakubu R., Han B., Tu V., Kato K., Kawazu S.-I., Gupta N., Suvorova E.S., White M.W., Kim K. and Weiss L.M.: Toxoplasma gondii cyclic AMP-dependent protein kinase subunit 3 Is involved in the switch from tachyzoite to bradyzoite development. mBio 2016; 7.","journal-title":"mBio"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2005642"},{"key":"e_1_3_5_20_2","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms10519","article-title":"Functional profiles of orphan membrane transporters in the life cycle of the malaria parasite","volume":"7","author":"Kenthirapalan S.","year":"2016","unstructured":"Kenthirapalan S., Waters A.P., Matuschewski K. and Kooij T.W.A.: Functional profiles of orphan membrane transporters in the life cycle of the malaria parasite. Nat Commun 2016; 7.","journal-title":"Nat Commun"},{"key":"e_1_3_5_21_2","article-title":"A cyclic GMP signalling module that regulates gliding motility in a malaria parasite","volume":"5","author":"Moon R.W.","year":"2009","unstructured":"Moon R.W., Taylor C.J., Bex C., Schepers R., Goulding D., Janse C.J., Water A.P., Baker D.A. and Billker O.: A cyclic GMP signalling module that regulates gliding motility in a malaria parasite. PLoS Pathogens 2009; 5. e1000599.","journal-title":"PLoS Pathogens"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06267.x"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2018.06.069"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004815"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-018-0339-8"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.201900402"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.005491"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02948.x"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.1.3.317-328.2002"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(01)00451-0"},{"key":"e_1_3_5_31_2","doi-asserted-by":"crossref","first-page":"15913","DOI":"10.1074\/jbc.M108393200","article-title":"Purification and molecular characterization of cGMP-dependent protein kinase from apicomplexan parasites: a novel chemotherapeutic target","volume":"277","author":"Gurnett A.M.","year":"2002","unstructured":"Gurnett A.M., Liberator P.A., Dulski P.M., Salowe S.P., Donald R.G.K., Anderson J.W., Wiltsie J., Diaz C.A., Harris G., Chang B., Darkin-Rattray S.J., Nare B., Crumley T., Blum P.S., Misura A.S., Tamas T., Sardana M.K., Yuan J., Biftu T. and Schmatz D.M.: Purification and molecular characterization of cGMP-dependent protein kinase from apicomplexan parasites: a novel chemotherapeutic target. J Biol Chem 2002; 277: 15913.","journal-title":"J Biol Chem"},{"key":"e_1_3_5_32_2","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-017-00572-x","article-title":"A potent series targeting the malarial cGMP-dependent protein kinase clears infection and blocks transmission","volume":"8","author":"Baker D.A.","year":"2017","unstructured":"Baker D.A., Stewart L.B., Large J.M., Bowyer P.W., Ansell K.H., Jim\u00e9nez-D\u00edaz M.B., El Bakkouri M., Birchall K., Dechering K.J., Bouloc N.S., Coombs P.J., Whalley D., Harding D.J., Smiljanic-Hurley E., Wheldon M.C., Walker E.M., Dessens J.T., Lafuente M.J., Sanz L.M., Gamo F.-J., Ferrer S.B., Hui R., Bousema T., Angulo-Bartur\u00e9n I., Merritt A.T., Croft S.L., Gutteridge W.E., Kettleborough C.A. and Osborne S.A.: A potent series targeting the malarial cGMP-dependent protein kinase clears infection and blocks transmission. Nat Commun 2017; 8.","journal-title":"Nat Commun"},{"key":"e_1_3_5_33_2","doi-asserted-by":"crossref","DOI":"10.26508\/lsa.201900626","article-title":"Simultaneous multiple allelic replacement in the malaria parasite enables dissection of PKG function","volume":"3","author":"Koussis K.","year":"2020","unstructured":"Koussis K., Withers-Martinez C., Baker D.A. and Blackman M.J.: Simultaneous multiple allelic replacement in the malaria parasite enables dissection of PKG function. Life Science Alliance 2020; 3.","journal-title":"Life Science Alliance"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00186-09"},{"key":"e_1_3_5_35_2","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00375-17","article-title":"Plasma membrane association by N-acylation governs PKG function in Toxoplasma gondii","volume":"8","author":"Brown K.M.","year":"2017","unstructured":"Brown K.M., Long S., Sibley L.D. and Weiss L.M.: Plasma membrane association by N-acylation governs PKG function in Toxoplasma gondii. mBio 2017; 8.","journal-title":"mBio"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000154"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2008.05.016"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20050425"},{"key":"e_1_3_5_39_2","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.02330-14","article-title":"Cyclic GMP balance is critical for malaria parasite transmission from the mosquito to the mammalian host","volume":"6","author":"Lakshmanan V.","year":"2015","unstructured":"Lakshmanan V., Fishbaugher M.E., Morrison B., Baldwin M., Macarulay M., Vaughan A.M. et al: Cyclic GMP balance is critical for malaria parasite transmission from the mosquito to the mammalian host. mBio 2015; 6.","journal-title":"mBio"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1021\/cb501004q"},{"key":"e_1_3_5_41_2","doi-asserted-by":"crossref","first-page":"D553","DOI":"10.1093\/nar\/gkm981","article-title":"ToxoDB: An integrated Toxoplasma gondii database resource","volume":"36","author":"Gajria B.","year":"2007","unstructured":"Gajria B., Bahl A., Brestelli J., Dommer J., Fischer S., Gao X. et al: ToxoDB: An integrated Toxoplasma gondii database resource. Nucleic acids research 2007; 36: D553.","journal-title":"Nucleic acids research"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.76.060305.150444"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-019-0033-4"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1124\/mol.65.2.267"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2015.09.006"},{"key":"e_1_3_5_46_2","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/978-3-642-17969-3_4","volume-title":"Phosphodiesterases as Drug Targets","author":"Ke H.","year":"2011","unstructured":"Ke H., Wang H. and Ye M.: Structural insight into the substrate specificity of phosphodiesterases. In: Phosphodiesterases as Drug Targets. : Springer 2011: 121."},{"key":"e_1_3_5_47_2","doi-asserted-by":"crossref","first-page":"5782","DOI":"10.1073\/pnas.0700279104","article-title":"Structural insight into substrate specificity of phosphodiesterase 10. Proceedings of the National Academy of SciencesStructural insight into substrate specificity of phosphodiesterase 10","volume":"104","author":"Wang H.","year":"2007","unstructured":"Wang H., Liu Y., Hou J., Zheng M., Robinson H. and Ke H.: Structural insight into substrate specificity of phosphodiesterase 10. Proceedings of the National Academy of SciencesStructural insight into substrate specificity of phosphodiesterase 10. Proceedings of the National Academy of Sciences 2007; 104: 5782.","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2005.07.003"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20050057"},{"key":"e_1_3_5_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2540"},{"key":"e_1_3_5_51_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.201310959"},{"key":"e_1_3_5_52_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0168361"},{"key":"e_1_3_5_53_2","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1111\/jeu.12626","article-title":"Tagging of weakly expressed Toxoplasma gondii calcium-related genes with high-affinity tags","volume":"65","author":"Hortua Triana M.A.","year":"2018","unstructured":"Hortua Triana M.A., M\u00e1rquez-Nogueras K.M., Chang L., Stasic A.J., Li C., Spiegel K.A. et al: Tagging of weakly expressed Toxoplasma gondii calcium-related genes with high-affinity tags. J Eukaryot Microbiol 2018; 65: 709.","journal-title":"J Eukaryot Microbiol"},{"key":"e_1_3_5_54_2","first-page":"1","article-title":"Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes","volume":"16","author":"Hehl A.B.","year":"2015","unstructured":"Hehl A.B., Basso W.U., Lippuner C., Ramakrishnan C., Okoniewski M., Walker R.A. et al: Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes. BMC Genomics 2015; 16: 1.","journal-title":"BMC Genomics"},{"key":"e_1_3_5_55_2","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1001094","article-title":"A novel family of Toxoplasma IMC proteins displays a hierarchical organization and functions in coordinating parasite division","volume":"6","author":"Beck J.R.","year":"2010","unstructured":"Beck J.R., Rodriguez-Fernandez I.A., De Leon J.C., Huynh M.-H., Carruthers V.B., Morrissette N.S. et al: A novel family of Toxoplasma IMC proteins displays a hierarchical organization and functions in coordinating parasite division. PLoS Pathogens 2010; 6: e1001094.","journal-title":"PLoS Pathogens"},{"key":"e_1_3_5_56_2","first-page":"3","article-title":"Cyclic nucleotide phosphodiesterase superfamily","author":"Beavo J.","year":"2007","unstructured":"Beavo J., Houslay M. and Francis S.: Cyclic nucleotide phosphodiesterase superfamily. Cyclic Nucleotide Phosphodiesterases in Health and Disease 2007; : 3.","journal-title":"Cyclic Nucleotide Phosphodiesterases in Health and Disease"},{"key":"e_1_3_5_57_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708850105"},{"key":"e_1_3_5_58_2","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.jmb.2007.05.060","article-title":"The molecular basis for different recognition of substrates by phosphodiesterase families 4 and 10","volume":"371","author":"Wang H.","year":"2007","unstructured":"Wang H., Robinson H. and Ke H.: The molecular basis for different recognition of substrates by phosphodiesterase families 4 and 10. J Mol Biol 2007; 371: 302.","journal-title":"J Mol Biol"},{"key":"e_1_3_5_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.07.005"},{"key":"e_1_3_5_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.019"},{"key":"e_1_3_5_61_2","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1084\/jem.20011876","article-title":"MAEBL is essential for malarial sporozoite infection of the mosquito salivary gland","volume":"195","author":"Kariu T.","year":"2002","unstructured":"Kariu T., Yuda M., Yano K. and Chinzei Y.: MAEBL is essential for malarial sporozoite infection of the mosquito salivary gland. J Exp Med 2002; 195: 1317.","journal-title":"J Exp Med"},{"key":"e_1_3_5_62_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12745"},{"key":"e_1_3_5_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(02)00067-1"},{"key":"e_1_3_5_64_2","doi-asserted-by":"crossref","DOI":"10.1016\/j.pharmthera.2020.107475","article-title":"Cyclic nucleotide phosphodiesterases: New targets in the metabolic syndrome?","volume":"208","author":"Lugnier C.","year":"2020","unstructured":"Lugnier C., Meyer A., Talha S. and Geny B.: Cyclic nucleotide phosphodiesterases: New targets in the metabolic syndrome?. Pharmacol Ther 2020; 208: 107475.","journal-title":"Pharmacol Ther"},{"key":"e_1_3_5_65_2","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1124\/mol.111.076125","article-title":"cAMP-specific phosphodiesterases 8A and 8B, essential regulators of Leydig cell steroidogenesis","volume":"81","author":"Shimizu-Albergine M.","year":"2012","unstructured":"Shimizu-Albergine M., Tsai L.-C.-L., Patrucco E. and Beavo J.A.: cAMP-specific phosphodiesterases 8A and 8B, essential regulators of Leydig cell steroidogenesis. Mol Pharmacol 2012; 81: 556.","journal-title":"Mol Pharmacol"},{"key":"e_1_3_5_66_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuz005"},{"key":"e_1_3_5_67_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00357-08"},{"key":"e_1_3_5_68_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00358-08"},{"key":"e_1_3_5_69_2","doi-asserted-by":"crossref","first-page":"14010","DOI":"10.1074\/jbc.271.24.14010","article-title":"Insertional tagging, cloning, and expression of the Toxoplasma gondii hypoxanthine-xanthine-guanine phosphoribosyltransferase gene Use as a selectable marker for stable transformation","volume":"271","author":"Donald R.G.","year":"1996","unstructured":"Donald R.G., Carter D., Ullman B. and Roos D.S.: Insertional tagging, cloning, and expression of the Toxoplasma gondii hypoxanthine-xanthine-guanine phosphoribosyltransferase gene Use as a selectable marker for stable transformation. J Biol Chem 1996; 271: 14010.","journal-title":"J Biol Chem"},{"key":"e_1_3_5_70_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3365"},{"key":"e_1_3_5_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2008.01.001"},{"key":"e_1_3_5_72_2","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/j.jmb.2005.05.031","article-title":"Toxoplasma gondii Hsp90 is a potential drug target whose expression and subcellular localization are developmentally regulated","volume":"350","author":"Echeverria P.C.","year":"2005","unstructured":"Echeverria P.C., Matrajt M., Harb O.S., Zappia M.P., Costas M.A., Roos D.S. et al: Toxoplasma gondii Hsp90 is a potential drug target whose expression and subcellular localization are developmentally regulated. J Mol Biol 2005; 350: 723.","journal-title":"J Mol Biol"},{"key":"e_1_3_5_73_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14411"},{"key":"e_1_3_5_74_2","doi-asserted-by":"crossref","first-page":"16345","DOI":"10.1074\/jbc.M501523200","article-title":"Selective disruption of phosphatidylcholine metabolism of the intracellular parasite Toxoplasma gondii arrests its growth","volume":"280","author":"Gupta N.","year":"2005","unstructured":"Gupta N., Zahn M.M., Coppens I., Joiner K.A. and Voelker D.R.: Selective disruption of phosphatidylcholine metabolism of the intracellular parasite Toxoplasma gondii arrests its growth. J Biol Chem 2005; 280: 16345.","journal-title":"J Biol Chem"},{"key":"e_1_3_5_75_2","doi-asserted-by":"crossref","first-page":"11703","DOI":"10.1073\/pnas.90.24.11703","article-title":"Stable molecular transformation of Toxoplasma gondii: a selectable dihydrofolate reductase-thymidylate synthase marker based on drug-resistance mutations in malaria","volume":"90","author":"Donald R.","year":"1993","unstructured":"Donald R. and Roos D.S.: Stable molecular transformation of Toxoplasma gondii: a selectable dihydrofolate reductase-thymidylate synthase marker based on drug-resistance mutations in malaria. Proc Natl Acad Sci 1993; 90: 11703.","journal-title":"Proc Natl Acad Sci"},{"key":"e_1_3_5_76_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1105"},{"key":"e_1_3_5_77_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv362"},{"key":"e_1_3_5_78_2","first-page":"175","article-title":"A hidden Markov model for predicting transmembrane helices in protein sequences","volume":"6","author":"Sonnhammer E.L.","year":"1998","unstructured":"Sonnhammer E.L., Von Heijne G. and Krogh A.: A hidden Markov model for predicting transmembrane helices in protein sequences. ISMB 1998; 6: 175.","journal-title":"ISMB"},{"key":"e_1_3_5_79_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.03.016"},{"key":"e_1_3_5_80_2","first-page":"166","article-title":"TMbase-A database of membrane spanning proteins segments","volume":"374","author":"Hofmann K.","year":"1993","unstructured":"Hofmann K.: TMbase-A database of membrane spanning proteins segments. Biol Chem Hoppe-Seyler 1993; 374: 166.","journal-title":"Biol Chem Hoppe-Seyler"},{"key":"e_1_3_5_81_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh454"},{"key":"e_1_3_5_82_2","doi-asserted-by":"crossref","first-page":"D257","DOI":"10.1093\/nar\/gku949","article-title":"SMART: recent updates, new developments and status in 2015","volume":"43","author":"Letunic I.","year":"2014","unstructured":"Letunic I., Doerks T. and Bork P.: SMART: recent updates, new developments and status in 2015. Nucleic Acids Res 2014; 43: D257.","journal-title":"Nucleic Acids Res"},{"key":"e_1_3_5_83_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky995"},{"key":"e_1_3_5_84_2","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1093\/bioinformatics\/16.11.1050","article-title":"TEXtopo: shaded membrane protein topology plots in LATEX2\u03b5","volume":"16","author":"Beitz E.","year":"2000","unstructured":"Beitz E.: TEXtopo: shaded membrane protein topology plots in LATEX2\u03b5. Bioinformatics 2000; 16: 1050.","journal-title":"Bioinformatics"},{"key":"e_1_3_5_85_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"e_1_3_5_86_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz239"},{"key":"e_1_3_5_87_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky427"},{"key":"e_1_3_5_88_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21334"},{"key":"e_1_3_5_89_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20084"}],"container-title":["Computational and Structural Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2001037020304918?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2001037020304918?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/spj.science.org\/doi\/pdf\/10.1016\/j.csbj.2020.11.024","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T12:38:07Z","timestamp":1779021487000},"score":1,"resource":{"primary":{"URL":"https:\/\/spj.science.org\/doi\/10.1016\/j.csbj.2020.11.024"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":88,"alternative-id":["10.1016\/j.csbj.2020.11.024"],"URL":"https:\/\/doi.org\/10.1016\/j.csbj.2020.11.024","relation":{},"ISSN":["2001-0370"],"issn-type":[{"value":"2001-0370","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The protozoan parasite Toxoplasma gondii encodes a gamut of phosphodiesterases during its lytic cycle in human cells","name":"articletitle","label":"Article Title"},{"value":"Computational and Structural Biotechnology Journal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.csbj.2020.11.024","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Author(s). Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology.","name":"copyright","label":"Copyright"}]}}