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However, there has been limited application of such technology to related parasitic nematodes which cause major diseases of humans and animals worldwide. With no vaccines against the vast majority of parasitic nematodes and treatment failures due to drug resistance or inefficacy, new intervention targets are urgently needed, preferably informed by a deep understanding of these nematodes\u2019 cellular and molecular biology \u2013 which is presently lacking for most worms. Here, we created the first single cell atlas for an early developmental stage of\n                    <jats:italic toggle=\"yes\">Haemonchus contortus \u2013<\/jats:italic>\n                    a highly pathogenic,\n                    <jats:italic toggle=\"yes\">C. elegans<\/jats:italic>\n                    -related parasitic nematode. We obtained and curated RNA sequence (snRNA-seq) data from single nuclei from embryonating eggs of\n                    <jats:italic toggle=\"yes\">H. contortus<\/jats:italic>\n                    (150,000 droplets), and selected high-quality transcriptomic data for &gt;\u00a014,000 single nuclei for analysis, and identified 19 distinct clusters of cells. Guided by comparative analyses with\n                    <jats:italic toggle=\"yes\">C. elegans<\/jats:italic>\n                    , we were able to reproducibly assign seven cell clusters to body wall muscle, hypodermis, neuronal, intestinal or seam cells, and identified eight genes that were transcribed in all cell clusters\/types, three of which were inferred to be essential in\n                    <jats:italic toggle=\"yes\">H. contortus<\/jats:italic>\n                    . Two of these genes (i.e.\n                    <jats:italic toggle=\"yes\">Hc-eef-1A<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Hc-eef1G<\/jats:italic>\n                    ), coding for eukaryotic elongation factors (called\n                    <jats:italic toggle=\"yes\">Hc-<\/jats:italic>\n                    eEF1A and\n                    <jats:italic toggle=\"yes\">Hc<\/jats:italic>\n                    -eEF1G), were also demonstrated to be transcribed and expressed in all key developmental stages of\n                    <jats:italic toggle=\"yes\">H. contortus.<\/jats:italic>\n                    Together with these findings, sequence- and structure-based comparative analyses indicated the potential of\n                    <jats:italic toggle=\"yes\">Hc<\/jats:italic>\n                    -eEF1A and\/or\n                    <jats:italic toggle=\"yes\">Hc<\/jats:italic>\n                    -eEF1G as intervention targets within the protein biosynthesis machinery of\n                    <jats:italic toggle=\"yes\">H. contortus<\/jats:italic>\n                    . Future work will focus on single cell studies of all key developmental stages and tissues of\n                    <jats:italic toggle=\"yes\">H. contortus<\/jats:italic>\n                    , and on evaluating the suitability of the two elongation factor proteins as drug targets in\n                    <jats:italic toggle=\"yes\">H. contortus<\/jats:italic>\n                    and related nematodes, with a view to finding new nematocidal drug candidates.\n                  <\/jats:p>","DOI":"10.1016\/j.csbj.2024.01.008","type":"journal-article","created":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T16:55:05Z","timestamp":1705510505000},"page":"1026-1035","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"title":["Analysis of\n                    <i>Haemonchus<\/i>\n                    embryos at single cell resolution identifies two eukaryotic elongation factors as intervention target candidates"],"prefix":"10.34133","volume":"23","author":[{"given":"Pasi K.","family":"Korhonen","sequence":"first","affiliation":[{"name":"Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil D.","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph J.","family":"Byrne","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tulio L.","family":"Campos","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bill C.H.","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aya C.","family":"Taki","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin B.","family":"Gasser","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","published-online":{"date-parts":[[2024,1,17]]},"reference":[{"key":"e_1_4_2_2_2","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1534\/genetics.109.104976","article-title":"In the beginning was the worm","volume":"182","author":"Brenner S.","year":"2009","unstructured":"BrennerS.In the beginning was the wormGenetics182200941341519506024","journal-title":"Genetics"},{"key":"e_1_4_2_3_2","volume-title":"The C. elegans Research Community","author":"Nigon V.M.","year":"2017","unstructured":"Nigon V.M. 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