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They employ diverse metabolic pathways, allowing them to adapt to various growth conditions and even extreme environments. Thus, they are ideal organisms for the Next Generation Industrial Biotechnology concept of reducing the risk of contamination by using naturally robust extremophiles. Unfortunately, the potential of PPB for use in biotechnology is hampered by missing knowledge on regulations of their metabolism. Although\n                    <jats:italic toggle=\"yes\">Rhodospirillum rubrum<\/jats:italic>\n                    represents a model purple bacterium studied for polyhydroxyalkanoate and hydrogen production, light\/chemical energy conversion, and nitrogen fixation, little is known regarding the regulation of its metabolism at the transcriptomic level. Using RNA sequencing, we compared gene expression during the cultivation utilizing fructose and acetate as substrates in case of the wild-type strain\n                    <jats:italic toggle=\"yes\">R. rubrum<\/jats:italic>\n                    DSM 467\n                    <jats:sup>T<\/jats:sup>\n                    and its knock-out mutant strain that is missing two polyhydroxyalkanoate synthases PhaC1 and PhaC2. During this first genome-wide expression study of\n                    <jats:italic toggle=\"yes\">R. rubrum<\/jats:italic>\n                    , we were able to characterize cultivation-driven transcriptomic changes and to annotate non-coding elements as small RNAs.\n                  <\/jats:p>","DOI":"10.1016\/j.csbj.2024.06.023","type":"journal-article","created":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T13:28:19Z","timestamp":1718890099000},"page":"2681-2694","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"title":["Cultivation driven transcriptomic changes in the wild-type and mutant strains of\n                    <i>Rhodospirillum rubrum<\/i>"],"prefix":"10.34133","volume":"23","author":[{"given":"Katerina","family":"Jureckova","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Brno, Czech 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