{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T03:47:15Z","timestamp":1791863235495,"version":"4.3.4"},"reference-count":42,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:00:00Z","timestamp":1790380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Stress and Chaperones"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.cstres.2026.100221","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T15:17:43Z","timestamp":1790781463000},"page":"100221","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["The barcoded binding assay: Multiplexed profiling of chaperone binding selectivity for hydrophobic substrates"],"prefix":"10.1016","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3025-1076","authenticated-orcid":false,"given":"Shawn P.","family":"Allen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9406-3410","authenticated-orcid":false,"given":"Matthew L.","family":"Wohlever","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cstres.2026.100221_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.sbi.2022.102381","article-title":"Computational design of transmembrane proteins","volume":"74","author":"Zhu","year":"2022","journal-title":"Curr Opin Struct Biol"},{"key":"10.1016\/j.cstres.2026.100221_bib2","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1091\/mbc.e07-07-0677","article-title":"Divergent regulation of protein synthesis in the cytosol and endoplasmic reticulum compartments of mammalian cells","volume":"19","author":"Stephens","year":"2008","journal-title":"Mol Biol Cell"},{"key":"10.1016\/j.cstres.2026.100221_bib3","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1038\/s41580-021-00413-2","article-title":"The mechanisms of integral membrane protein biogenesis","volume":"23","author":"Hegde","year":"2022","journal-title":"Nat Rev Mol Cell Bio"},{"key":"10.1016\/j.cstres.2026.100221_bib4","doi-asserted-by":"crossref","first-page":"S10","DOI":"10.1038\/nm1066","article-title":"Protein aggregation and neurodegenerative disease","volume":"10","author":"Ross","year":"2004","journal-title":"Nat Med"},{"key":"10.1016\/j.cstres.2026.100221_bib5","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.molcel.2017.06.019","article-title":"Msp1 Is a membrane protein dislocase for tail-anchored proteins","volume":"67","author":"Wohlever","year":"2017","journal-title":"Mol Cell"},{"key":"10.1016\/j.cstres.2026.100221_bib6","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.mito.2019.07.011","article-title":"Sorting out how Msp1 maintains mitochondrial membrane proteostasis","volume":"49","author":"Fresenius","year":"2019","journal-title":"Mitochondrion"},{"key":"10.1016\/j.cstres.2026.100221_bib7","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.15252\/embj.201487943","article-title":"Msp1\/ATAD1 maintains mitochondrial function by facilitating the degradation of mislocalized tail-anchored proteins","volume":"33","author":"Chen","year":"2014","journal-title":"EMBO J"},{"key":"10.1016\/j.cstres.2026.100221_bib8","doi-asserted-by":"crossref","DOI":"10.15252\/embj.2022112309","article-title":"MitoStores: chaperone-controlled protein granules store mitochondrial precursors in the cytosol","volume":"42","author":"Kr\u00e4mer","year":"2023","journal-title":"Embo J"},{"key":"10.1016\/j.cstres.2026.100221_bib9","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.82860","article-title":"Collateral deletion of the mitochondrial AAA+ ATPase ATAD1 sensitizes cancer cells to proteasome dysfunction","volume":"11","author":"Winter","year":"2022","journal-title":"Elife"},{"key":"10.1016\/j.cstres.2026.100221_bib10","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1083\/jcb.201005144","article-title":"Protein homeostasis and aging in neurodegeneration","volume":"190","author":"Douglas","year":"2010","journal-title":"J Cell Biol"},{"key":"10.1016\/j.cstres.2026.100221_bib11","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1146\/annurev-biochem-060614-033955","article-title":"The biology of proteostasis in aging and disease","volume":"84","author":"Labbadia","year":"2015","journal-title":"Annu Rev Biochem"},{"key":"10.1016\/j.cstres.2026.100221_bib12","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1146\/annurev-biochem-080125-020218","article-title":"Membrane protein insertion in mammalian cells","volume":"95","author":"Guna","year":"2026","journal-title":"Annu Rev Biochem"},{"key":"10.1016\/j.cstres.2026.100221_bib13","article-title":"Recognition and degradation of mislocalized proteins in health and disease","volume":"11","author":"Hegde","year":"2019","journal-title":"Csh Perspect Biol"},{"key":"10.1016\/j.cstres.2026.100221_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2025.110614","article-title":"Lipid bilayer properties govern substrate engagement and extraction by the AAA+ ATPase Msp1","volume":"301","author":"Fresenius","year":"2025","journal-title":"J Biol Chem"},{"key":"10.1016\/j.cstres.2026.100221_bib15","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1002\/1873-3468.70187","article-title":"Linked dimers of the AAA+ ATPase Msp1 reveal energetic demands and mechanistic plasticity for substrate extraction from lipid bilayers","volume":"600","author":"Gaur","year":"2025","journal-title":"FEBS Lett"},{"key":"10.1016\/j.cstres.2026.100221_bib16","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1038\/s41594-024-01296-5","article-title":"A unifying model for membrane protein biogenesis","volume":"31","author":"Hegde","year":"2024","journal-title":"Nat Struct Mol Biol"},{"key":"10.1016\/j.cstres.2026.100221_bib17","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.molcel.2019.07.006","article-title":"Msp1 clears mistargeted proteins by facilitating their transfer from mitochondria to the ER","volume":"76","author":"Matsumoto","year":"2019","journal-title":"Mol Cell"},{"key":"10.1016\/j.cstres.2026.100221_bib18","doi-asserted-by":"crossref","first-page":"9851","DOI":"10.1073\/pnas.1207382109","article-title":"Hydrophobic forces and the length limit of foldable protein domains","volume":"109","author":"Lin","year":"2012","journal-title":"Proc Natl Acad Sci"},{"key":"10.1016\/j.cstres.2026.100221_bib19","doi-asserted-by":"crossref","first-page":"7425","DOI":"10.1021\/acs.analchem.7b00922","article-title":"Effects of detergent micelles on lipid binding to proteins in electrospray ionization mass spectrometry","volume":"89","author":"Landreh","year":"2017","journal-title":"Anal Chem"},{"key":"10.1016\/j.cstres.2026.100221_bib20","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/0304-4157(75)90016-7","article-title":"Solubilization of membranes by detergents","volume":"415","author":"Helenius","year":"1975","journal-title":"Biochim Biophys Acta (BBA) Rev Biomembr"},{"key":"10.1016\/j.cstres.2026.100221_bib21","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1042\/BST20210181","article-title":"Methods for the solubilisation of membrane proteins: the micelle-aneous world of membrane protein solubilisation","volume":"49","author":"Ratkeviciute","year":"2021","journal-title":"Biochem Soc Trans"},{"key":"10.1016\/j.cstres.2026.100221_bib22","doi-asserted-by":"crossref","first-page":"vbac002","DOI":"10.1093\/bioadv\/vbac002","article-title":"How sticky are our proteins? Quantifying hydrophobicity of the human proteome","volume":"2","author":"van Gils","year":"2022","journal-title":"Bioinform Adv"},{"key":"10.1016\/j.cstres.2026.100221_bib23","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1124\/dmd.31.7.815","article-title":"The conduct of in vitro and in vivo drug-drug interaction studies: a pharmaceutical research and manufacturers of America (PhRMA) perspective","volume":"31","author":"Bjornsson","year":"2003","journal-title":"Drug Metab Dispos"},{"key":"10.1016\/j.cstres.2026.100221_bib24","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1128\/mr.59.1.94-123.1995","article-title":"Protein-protein interactions: methods for detection and analysis","volume":"59","author":"Phizicky","year":"1995","journal-title":"Microbiol Rev"},{"key":"10.1016\/j.cstres.2026.100221_bib25","doi-asserted-by":"crossref","first-page":"2694","DOI":"10.1038\/s44318-026-00745-9","article-title":"ALS mutations disrupt self-association between the ubiquilin STI1 hydrophobic groove and internal placeholder sequences","volume":"45","author":"Onwunma","year":"2026","journal-title":"EMBO J"},{"key":"10.1016\/j.cstres.2026.100221_bib26","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.molcel.2016.05.020","article-title":"Ubiquilins chaperone and triage mitochondrial membrane proteins for degradation","volume":"63","author":"Itakura","year":"2016","journal-title":"Mol Cell"},{"key":"10.1016\/j.cstres.2026.100221_bib27","doi-asserted-by":"crossref","first-page":"3471","DOI":"10.1042\/BCJ20190497","article-title":"Structure, dynamics and functions of UBQLNs: at the crossroads of protein quality control machinery","volume":"477","author":"Zheng","year":"2020","journal-title":"Biochem J"},{"key":"10.1016\/j.cstres.2026.100221_bib28","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1002\/pro.4049","article-title":"The STI1-domain is a flexible alpha-helical fold with a hydrophobic groove","volume":"30","author":"Fry","year":"2021","journal-title":"Protein Sci"},{"key":"10.1016\/j.cstres.2026.100221_bib29","doi-asserted-by":"crossref","first-page":"2712","DOI":"10.1038\/s44318-026-00696-1","article-title":"STI1 domain engages transient helices to mediate Dsk2 phase separation and proteasome condensation","volume":"45","author":"Acharya","year":"2026","journal-title":"EMBO J"},{"key":"10.1016\/j.cstres.2026.100221_bib30","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1038\/90802","article-title":"Cell-free translation reconstituted with purified components","volume":"19","author":"Shimizu","year":"2001","journal-title":"Nat Biotechnol"},{"key":"10.1016\/j.cstres.2026.100221_bib31","series-title":"Radioecological Techniques","first-page":"17","author":"Schultz","year":"1982"},{"key":"10.1016\/j.cstres.2026.100221_bib32","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.cstres.2026.100221_bib33","doi-asserted-by":"crossref","DOI":"10.1002\/pro.70106","article-title":"A suite of pre-assembled, pET28b-based Golden Gate vectors for efficient protein engineering and expression","volume":"34","author":"Gaur","year":"2025","journal-title":"Protein Sci"},{"key":"10.1016\/j.cstres.2026.100221_bib34","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1083\/jcb.141.4.887","article-title":"The \u03b2 Subunit of the Sec61 complex facilitates cotranslational protein transport and interacts with the signal peptidase during translocation","volume":"141","author":"Kalies","year":"1998","journal-title":"J Cell Biol"},{"key":"10.1016\/j.cstres.2026.100221_bib35","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s12551-012-0081-z","article-title":"SH3 domains: modules of protein\u2013protein interactions","volume":"5","author":"Kurochkina","year":"2013","journal-title":"Biophys Rev"},{"key":"10.1016\/j.cstres.2026.100221_bib36","first-page":"1","article-title":"Protein modules in signal transduction","volume":"228","author":"Mayer","year":"1998","journal-title":"Curr Top Microbiol Immunol"},{"key":"10.1016\/j.cstres.2026.100221_bib37","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1126\/science.add1856","article-title":"MTCH2 is a mitochondrial outer membrane protein insertase","volume":"378","author":"Guna","year":"2022","journal-title":"Science"},{"key":"10.1016\/j.cstres.2026.100221_bib38","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0022-2836(82)90515-0","article-title":"A simple method for displaying the hydropathic character of a protein","volume":"157","author":"Kyte","year":"1982","journal-title":"J Mol Biol"},{"key":"10.1016\/j.cstres.2026.100221_bib39","article-title":"Ubiquilin 2 modulates ALS\/FTD-linked FUS\u2013RNA complex dynamics and stress granule formation","volume":"5","author":"Alexander","year":"2018","journal-title":"Proc Natl Acad Sci"},{"key":"10.1016\/j.cstres.2026.100221_bib40","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat Methods"},{"key":"10.1016\/j.cstres.2026.100221_bib41","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/bs.mie.2014.11.010","article-title":"Chapter seven affinity pull-down of proteins using anti-FLAG M2 agarose beads","volume":"559","author":"Gerace","year":"2015","journal-title":"Methods Enzymol"},{"key":"10.1016\/j.cstres.2026.100221_bib42","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1038\/nbt1088-1204","article-title":"A short polypeptide marker sequence useful for recombinant protein identification and purification","volume":"6","author":"Hopp","year":"1988","journal-title":"BioTechnol"}],"container-title":["Cell Stress and Chaperones"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1355814526000799?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1355814526000799?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T03:21:26Z","timestamp":1791861686000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1355814526000799"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":42,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["S1355814526000799"],"URL":"https:\/\/doi.org\/10.1016\/j.cstres.2026.100221","relation":{},"ISSN":["1355-8145"],"issn-type":[{"value":"1355-8145","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The barcoded binding assay: Multiplexed profiling of chaperone binding selectivity for hydrophobic substrates","name":"articletitle","label":"Article Title"},{"value":"Cell Stress and Chaperones","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cstres.2026.100221","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc. on behalf of Cell Stress Society International.","name":"copyright","label":"Copyright"}],"article-number":"100221"}}