{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:24:53Z","timestamp":1791149093979,"version":"4.1.0"},"reference-count":36,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T00:00:00Z","timestamp":1359676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2014,2,4]],"date-time":"2014-02-04T00:00:00Z","timestamp":1391472000000},"content-version":"vor","delay-in-days":368,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Current Biology"],"published-print":{"date-parts":[[2013,2]]},"DOI":"10.1016\/j.cub.2012.12.024","type":"journal-article","created":{"date-parts":[[2013,1,4]],"date-time":"2013-01-04T00:29:07Z","timestamp":1357259347000},"page":"196-203","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":39,"title":["A Novel Actin-Binding Motif in Las17\/WASP Nucleates Actin Filaments Independently of Arp2\/3"],"prefix":"10.1016","volume":"23","author":[{"given":"Agnieszka\u00a0N.","family":"Urbanek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam\u00a0P.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen\u00a0G.","family":"Allwood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wesley\u00a0I.","family":"Booth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn\u00a0R.","family":"Ayscough","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cub.2012.12.024_bib1","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1242\/jcs.01670","article-title":"Actin-binding proteins","volume":"118","author":"Winder","year":"2005","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.cub.2012.12.024_bib2","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1038\/nrm2867","article-title":"A nucleator arms race: cellular control of actin assembly","volume":"11","author":"Campellone","year":"2010","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.cub.2012.12.024_bib3","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1083\/jcb.127.1.107","article-title":"Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agarose","volume":"127","author":"Machesky","year":"1994","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.cub.2012.12.024_bib4","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1038\/ncb834","article-title":"An actin nucleation mechanism mediated by Bni1 and profilin","volume":"4","author":"Sagot","year":"2002","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.cub.2012.12.024_bib5","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.ceb.2008.12.001","article-title":"Actin nucleation and elongation factors: mechanisms and interplay","volume":"21","author":"Chesarone","year":"2009","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.cub.2012.12.024_bib6","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1038\/nsmb870","article-title":"Conformational changes in the Arp2\/3 complex leading to actin nucleation","volume":"12","author":"Rodal","year":"2005","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.cub.2012.12.024_bib7","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.tcb.2009.03.004","article-title":"New players in actin polymerization\u2014WH2-domain-containing actin nucleators","volume":"19","author":"Qualmann","year":"2009","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.cub.2012.12.024_bib8","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.cell.2007.08.030","article-title":"Cordon-bleu is an actin nucleation factor and controls neuronal morphology","volume":"131","author":"Ahuja","year":"2007","journal-title":"Cell"},{"key":"10.1016\/j.cub.2012.12.024_bib9","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1126\/science.1155313","article-title":"Leiomodin is an actin filament nucleator in muscle cells","volume":"320","author":"Chereau","year":"2008","journal-title":"Science"},{"key":"10.1016\/j.cub.2012.12.024_bib10","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1038\/nature03241","article-title":"Drosophila Spire is an actin nucleation factor","volume":"433","author":"Quinlan","year":"2005","journal-title":"Nature"},{"key":"10.1016\/j.cub.2012.12.024_bib11","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1083\/jcb.136.3.649","article-title":"Bee1, a yeast protein with homology to Wiscott-Aldrich syndrome protein, is critical for the assembly of cortical actin cytoskeleton","volume":"136","author":"Li","year":"1997","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.cub.2012.12.024_bib12","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/S0960-9822(99)80218-8","article-title":"Activation of the yeast Arp2\/3 complex by Bee1p, a WASP-family protein","volume":"9","author":"Winter","year":"1999","journal-title":"Curr. Biol."},{"key":"10.1016\/j.cub.2012.12.024_bib13","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1371\/journal.pbio.0060001","article-title":"Distinct roles for Arp2\/3 regulators in actin assembly and endocytosis","volume":"6","author":"Galletta","year":"2008","journal-title":"PLoS Biol."},{"key":"10.1016\/j.cub.2012.12.024_bib14","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.devcel.2006.05.008","article-title":"Endocytic internalization in budding yeast requires coordinated actin nucleation and myosin motor activity","volume":"11","author":"Sun","year":"2006","journal-title":"Dev. Cell"},{"key":"10.1016\/j.cub.2012.12.024_bib15","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1016\/S0960-9822(03)00383-X","article-title":"Negative regulation of yeast WASp by two SH3 domain-containing proteins","volume":"13","author":"Rodal","year":"2003","journal-title":"Curr. Biol."},{"key":"10.1016\/j.cub.2012.12.024_bib16","doi-asserted-by":"crossref","first-page":"4045","DOI":"10.1091\/mbc.E02-05-0294","article-title":"Motility determinants in WASP family proteins","volume":"13","author":"Yarar","year":"2002","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.cub.2012.12.024_bib17","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1038\/nsb0195-28","article-title":"Defining protein interactions with yeast actin in\u00a0vivo","volume":"2","author":"Amberg","year":"1995","journal-title":"Nat. Struct. Biol."},{"key":"10.1016\/j.cub.2012.12.024_bib18","doi-asserted-by":"crossref","first-page":"e1000218","DOI":"10.1371\/journal.pbio.1000218","article-title":"Bayesian modeling of the yeast SH3 domain interactome predicts spatiotemporal dynamics of endocytosis proteins","volume":"7","author":"Tonikian","year":"2009","journal-title":"PLoS Biol."},{"key":"10.1016\/j.cub.2012.12.024_bib19","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/S0962-8924(00)01876-6","article-title":"Cell motility: proline-rich proteins promote protrusions","volume":"11","author":"Holt","year":"2001","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.cub.2012.12.024_bib20","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1111\/j.1432-1033.1985.tb09099.x","article-title":"Poly(L-proline)-binding proteins from chick embryos are a profilin and a profilactin","volume":"151","author":"Tanaka","year":"1985","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.cub.2012.12.024_bib21","doi-asserted-by":"crossref","first-page":"4103","DOI":"10.1111\/j.1742-4658.2007.05936.x","article-title":"Verprolin function in endocytosis and actin organization. Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain","volume":"274","author":"Thanabalu","year":"2007","journal-title":"FEBS J."},{"key":"10.1016\/j.cub.2012.12.024_bib22","doi-asserted-by":"crossref","first-page":"15212","DOI":"10.1021\/bi991843+","article-title":"Influence of the C terminus of Wiskott-Aldrich syndrome protein (WASp) and the Arp2\/3 complex on actin polymerization","volume":"38","author":"Higgs","year":"1999","journal-title":"Biochemistry"},{"key":"10.1016\/j.cub.2012.12.024_bib23","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1083\/jcb.200811116","article-title":"Regulators of yeast endocytosis identified by systematic quantitative analysis","volume":"185","author":"Burston","year":"2009","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.cub.2012.12.024_bib24","doi-asserted-by":"crossref","first-page":"2433","DOI":"10.1016\/j.febslet.2005.03.043","article-title":"The yeast eIF4E-associated protein Eap1p attenuates GCN4 translation upon TOR-inactivation","volume":"579","author":"Matsuo","year":"2005","journal-title":"FEBS Lett."},{"key":"10.1016\/j.cub.2012.12.024_bib25","doi-asserted-by":"crossref","first-page":"16644","DOI":"10.1073\/pnas.0507021102","article-title":"Actin-bound structures of Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 and the implications for filament assembly","volume":"102","author":"Chereau","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cub.2012.12.024_bib26","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1038\/365810a0","article-title":"The structure of crystalline profilin-beta-actin","volume":"365","author":"Schutt","year":"1993","journal-title":"Nature"},{"key":"10.1016\/j.cub.2012.12.024_bib27","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.str.2005.12.011","article-title":"The structural basis of actin interaction with multiple WH2\/beta-thymosin motif-containing proteins","volume":"14","author":"Aguda","year":"2006","journal-title":"Structure"},{"key":"10.1016\/j.cub.2012.12.024_bib28","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.cell.2005.09.024","article-title":"A modular design for\u00a0the clathrin- and actin-mediated endocytosis machinery","volume":"123","author":"Kaksonen","year":"2005","journal-title":"Cell"},{"key":"10.1016\/j.cub.2012.12.024_bib29","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.1007\/s00018-009-0001-y","article-title":"Functions of actin in endocytosis","volume":"66","author":"Robertson","year":"2009","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.cub.2012.12.024_bib30","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1038\/ncb1918","article-title":"Differential requirements for actin during yeast and mammalian endocytosis","volume":"11","author":"Aghamohammadzadeh","year":"2009","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.cub.2012.12.024_bib31","doi-asserted-by":"crossref","first-page":"4256","DOI":"10.1091\/mbc.E11-12-1022","article-title":"SLAC, a complex between Sla1 and Las17, regulates actin polymerization during clathrin-mediated endocytosis","volume":"23","author":"Feliciano","year":"2012","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.cub.2012.12.024_bib32","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1038\/nature02046","article-title":"Global analysis of protein expression in yeast","volume":"425","author":"Ghaemmaghami","year":"2003","journal-title":"Nature"},{"key":"10.1016\/j.cub.2012.12.024_bib33","series-title":"Methods in Yeast Genetics: A Laboratory Course Manual","author":"Kaiser","year":"1994"},{"key":"10.1016\/j.cub.2012.12.024_bib34","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1242\/jcs.108.1.63","article-title":"Utrophin actin binding domain: analysis of actin binding and cellular targeting","volume":"108","author":"Winder","year":"1995","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.cub.2012.12.024_bib35","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1016\/0076-6879(91)94051-D","article-title":"Yeast endocytosis assays","volume":"194","author":"Dulic","year":"1991","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.cub.2012.12.024_bib36","series-title":"Protein Localization by Fluorescence Microscopy: A Practical Approach","first-page":"179","article-title":"Fluorescence Microscopy in\u00a0Yeast","author":"Hagan","year":"2000"}],"container-title":["Current Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960982212014972?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960982212014972?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,2,5]],"date-time":"2022-02-05T05:17:31Z","timestamp":1644038251000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960982212014972"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,2]]},"references-count":36,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["S0960982212014972"],"URL":"https:\/\/doi.org\/10.1016\/j.cub.2012.12.024","relation":{},"ISSN":["0960-9822"],"issn-type":[{"value":"0960-9822","type":"print"}],"subject":[],"published":{"date-parts":[[2013,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A Novel Actin-Binding Motif in Las17\/WASP Nucleates Actin Filaments Independently of Arp2\/3","name":"articletitle","label":"Article Title"},{"value":"Current Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cub.2012.12.024","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}