{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T18:26:37Z","timestamp":1793039197107,"version":"4.5.0"},"reference-count":79,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,3,17]],"date-time":"2021-03-17T00:00:00Z","timestamp":1615939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000272","name":"NIHR","doi-asserted-by":"publisher","award":["NIHR2000907"],"award-info":[{"award-number":["NIHR2000907"]}],"id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI124452"],"award-info":[{"award-number":["R21AI124452"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI129507"],"award-info":[{"award-number":["R21AI129507"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["101285"],"award-info":[{"award-number":["101285"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000769","name":"University of Oxford","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"BBSRC","doi-asserted-by":"publisher","award":["BB\/T001240\/1"],"award-info":[{"award-number":["BB\/T001240\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"BBSRC","doi-asserted-by":"publisher","award":["V011278\/1"],"award-info":[{"award-number":["V011278\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014013","name":"UKRI","doi-asserted-by":"publisher","award":["20197"],"award-info":[{"award-number":["20197"]}],"id":[{"id":"10.13039\/100014013","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002141","name":"Public Health England","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007878","name":"Diabetes Research Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007878","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014976","name":"Liverpool School of Tropical Medicine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014976","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000276","name":"Department of Health and Social Care","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000276","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"publisher","award":["RSWF\\\\R1\\\\180013"],"award-info":[{"award-number":["RSWF\\\\R1\\\\180013"]}],"id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"publisher","award":["R21AI124452"],"award-info":[{"award-number":["R21AI124452"]}],"id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"publisher","award":["R21AI129507"],"award-info":[{"award-number":["R21AI129507"]}],"id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Current Biology"],"published-print":{"date-parts":[[2021,6]]},"DOI":"10.1016\/j.cub.2021.03.056","type":"journal-article","created":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T04:31:14Z","timestamp":1618461074000},"page":"2310-2320.e5","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":87,"title":["Stable high-density and maternally inherited Wolbachia infections in Anopheles moucheti and Anopheles demeilloni mosquitoes"],"prefix":"10.1016","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3545-012X","authenticated-orcid":false,"given":"Thomas","family":"Walker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon","family":"Quek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0298-2808","authenticated-orcid":false,"given":"Claire L.","family":"Jeffries","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janvier","family":"Bandibabone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7733-1356","authenticated-orcid":false,"given":"Vishaal","family":"Dhokiya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Bamou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0363-8558","authenticated-orcid":false,"given":"Mojca","family":"Kristan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louisa A.","family":"Messenger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0219-0456","authenticated-orcid":false,"given":"Alexandra","family":"Gidley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7747-0583","authenticated-orcid":false,"given":"Emily A.","family":"Hornett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8764-6706","authenticated-orcid":false,"given":"Enyia R.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cintia","family":"Cansado-Utrilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivanand","family":"Hegde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chimanuka","family":"Bantuzeko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer C.","family":"Stevenson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil F.","family":"Lobo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0577-5537","authenticated-orcid":false,"given":"Simon C.","family":"Wagstaff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe Antonio","family":"Nkondjio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8709-8247","authenticated-orcid":false,"given":"Seth R.","family":"Irish","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4413-3756","authenticated-orcid":false,"given":"Eva","family":"Heinz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7567-7185","authenticated-orcid":false,"given":"Grant L.","family":"Hughes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cub.2021.03.056_bib1","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1038\/s41586-019-1407-9","article-title":"Incompatible and sterile insect techniques combined eliminate mosquitoes","volume":"572","author":"Zheng","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.cub.2021.03.056_bib2","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1038\/s41587-020-0471-x","article-title":"Efficient production of male Wolbachia-infected Aedes aegypti mosquitoes enables large-scale suppression of wild populations","volume":"38","author":"Crawford","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.cub.2021.03.056_bib3","doi-asserted-by":"crossref","first-page":"50","DOI":"10.12688\/gatesopenres.13122.1","article-title":"Reduced dengue incidence following deployments of Wolbachia-infected Aedes aegypti in Yogyakarta, Indonesia: a quasi-experimental trial using controlled interrupted time series analysis","volume":"4","author":"Indriani","year":"2020","journal-title":"Gates Open Res."},{"key":"10.1016\/j.cub.2021.03.056_bib4","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1038\/nature10356","article-title":"Successful establishment of Wolbachia in Aedes populations to suppress dengue transmission","volume":"476","author":"Hoffmann","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.cub.2021.03.056_bib5","doi-asserted-by":"crossref","first-page":"4241","DOI":"10.1016\/j.cub.2019.11.007","article-title":"Establishment of Wolbachia strain wAlbB in Malaysian populations of Aedes aegypti for dengue control","volume":"29","author":"Nazni","year":"2019","journal-title":"Curr. Biol."},{"key":"10.1016\/j.cub.2021.03.056_bib6","doi-asserted-by":"crossref","first-page":"22042","DOI":"10.1073\/pnas.2006781117","article-title":"Evaluating insecticide resistance across African districts to aid malaria control decisions","volume":"117","author":"Moyes","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cub.2021.03.056_bib7","doi-asserted-by":"crossref","first-page":"e1002043","DOI":"10.1371\/journal.ppat.1002043","article-title":"Wolbachia infections are virulent and inhibit the human malaria parasite Plasmodium falciparum in Anopheles gambiae","volume":"7","author":"Hughes","year":"2011","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.cub.2021.03.056_bib8","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1126\/science.1236192","article-title":"Wolbachia invades Anopheles stephensi populations and induces refractoriness to Plasmodium infection","volume":"340","author":"Bian","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.cub.2021.03.056_bib9","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1111\/imb.12066","article-title":"Transinfection: a method to investigate Wolbachia-host interactions and control arthropod-borne disease","volume":"23","author":"Hughes","year":"2014","journal-title":"Insect Mol. Biol."},{"issue":"Suppl 1","key":"10.1016\/j.cub.2021.03.056_bib10","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1590\/S0074-02762011000900026","article-title":"Can Wolbachia be used to control malaria?","volume":"106","author":"Walker","year":"2011","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.cub.2021.03.056_bib11","doi-asserted-by":"crossref","first-page":"3985","DOI":"10.1038\/ncomms4985","article-title":"Evidence of natural Wolbachia infections in field populations of Anopheles gambiae","volume":"5","author":"Baldini","year":"2014","journal-title":"Nat. Commun."},{"key":"10.1016\/j.cub.2021.03.056_bib12","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1186\/s13071-018-3249-y","article-title":"First report of natural Wolbachia infection in the malaria mosquito Anopheles arabiensis in Tanzania","volume":"11","author":"Baldini","year":"2018","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.cub.2021.03.056_bib13","doi-asserted-by":"crossref","first-page":"11772","DOI":"10.1038\/ncomms11772","article-title":"Wolbachia infections in natural Anopheles populations affect egg laying and negatively correlate with Plasmodium development","volume":"7","author":"Shaw","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.cub.2021.03.056_bib14","doi-asserted-by":"crossref","first-page":"12566","DOI":"10.1073\/pnas.1716181114","article-title":"Effect of naturally occurring Wolbachia in Anopheles gambiae s.l. mosquitoes from Mali on Plasmodium falciparum malaria transmission","volume":"114","author":"Gomes","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cub.2021.03.056_bib15","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1186\/s12936-018-2559-z","article-title":"First report of natural Wolbachia infection in wild Anopheles funestus population in Senegal","volume":"17","author":"Niang","year":"2018","journal-title":"Malar. J."},{"key":"10.1016\/j.cub.2021.03.056_bib16","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1186\/s13071-020-04277-x","article-title":"Natural Wolbachia infection in field-collected Anopheles and other mosquito species from Malaysia","volume":"13","author":"Wong","year":"2020","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.cub.2021.03.056_bib17","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1111\/eva.12804","article-title":"Natural Wolbachia infections are common in the major malaria vectors in Central Africa","volume":"12","author":"Ayala","year":"2019","journal-title":"Evol. Appl."},{"key":"10.1016\/j.cub.2021.03.056_bib18","doi-asserted-by":"crossref","first-page":"113","DOI":"10.12688\/wellcomeopenres.14765.2","article-title":"Novel Wolbachia strains in Anopheles malaria vectors from Sub-Saharan Africa","volume":"3","author":"Jeffries","year":"2018","journal-title":"Wellcome Open Res."},{"key":"10.1016\/j.cub.2021.03.056_bib19","doi-asserted-by":"crossref","first-page":"16242","DOI":"10.1038\/nmicrobiol.2016.242","article-title":"Relic DNA is abundant in soil and obscures estimates of soil microbial diversity","volume":"2","author":"Carini","year":"2016","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.cub.2021.03.056_bib20","doi-asserted-by":"crossref","first-page":"e00784-19","DOI":"10.1128\/mBio.00784-19","article-title":"Is Anopheles gambiae a natural host of Wolbachia?","volume":"10","author":"Chrostek","year":"2019","journal-title":"MBio"},{"key":"10.1016\/j.cub.2021.03.056_bib21","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1002\/ece3.6012","article-title":"An elusive endosymbiont: does Wolbachia occur naturally in Aedes aegypti?","volume":"10","author":"Ross","year":"2020","journal-title":"Ecol. Evol."},{"key":"10.1016\/j.cub.2021.03.056_bib22","doi-asserted-by":"crossref","first-page":"202032","DOI":"10.1098\/rsos.202032","article-title":"Evidence for natural hybridization and novel Wolbachia strain superinfections in the Anopheles gambiae complex from Guinea","volume":"8","author":"Jeffries","year":"2021","journal-title":"R. Soc. Open Sci."},{"key":"10.1016\/j.cub.2021.03.056_bib23","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1186\/1475-2875-7-120","article-title":"Population structure of the malaria vector Anopheles moucheti in the equatorial forest region of Africa","volume":"7","author":"Antonio-Nkondjio","year":"2008","journal-title":"Malar. J."},{"key":"10.1016\/j.cub.2021.03.056_bib24","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.cub.2021.03.056_bib25","doi-asserted-by":"crossref","first-page":"e5486","DOI":"10.7717\/peerj.5486","article-title":"A bioinformatics approach to identifying Wolbachia infections in arthropods","volume":"6","author":"Pascar","year":"2018","journal-title":"PeerJ"},{"key":"10.1016\/j.cub.2021.03.056_bib26","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1038\/nature24995","article-title":"Genetic diversity of the African malaria vector Anopheles gambiae","volume":"552","year":"2017","journal-title":"Nature"},{"key":"10.1016\/j.cub.2021.03.056_bib27","doi-asserted-by":"crossref","first-page":"17007","DOI":"10.1038\/nmicrobiol.2017.7","article-title":"A Wolbachia deubiquitylating enzyme induces cytoplasmic incompatibility","volume":"2","author":"Beckmann","year":"2017","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.cub.2021.03.056_bib28","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1038\/nature21391","article-title":"Prophage WO genes recapitulate and enhance Wolbachia-induced cytoplasmic incompatibility","volume":"543","author":"LePage","year":"2017","journal-title":"Nature"},{"key":"10.1016\/j.cub.2021.03.056_bib29","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1038\/s41467-017-02749-w","article-title":"Culex pipiens crossing type diversity is governed by an amplified and polymorphic operon of Wolbachia","volume":"9","author":"Bonneau","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.cub.2021.03.056_bib30","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1093\/gbe\/evy012","article-title":"Evolutionary genetics of cytoplasmic incompatibility genes cifA and cifB in prophage WO of Wolbachia","volume":"10","author":"Lindsey","year":"2018","journal-title":"Genome Biol. Evol."},{"key":"10.1016\/j.cub.2021.03.056_bib31","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1038\/nature10355","article-title":"The wMel Wolbachia strain blocks dengue and invades caged Aedes aegypti populations","volume":"476","author":"Walker","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.cub.2021.03.056_bib32","doi-asserted-by":"crossref","first-page":"e1005434","DOI":"10.1371\/journal.ppat.1005434","article-title":"Establishment of a Wolbachia superinfection in Aedes aegypti mosquitoes as a potential approach for future resistance management","volume":"12","author":"Joubert","year":"2016","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.cub.2021.03.056_bib33","doi-asserted-by":"crossref","first-page":"12498","DOI":"10.1073\/pnas.1408888111","article-title":"Native microbiome impedes vertical transmission of Wolbachia in Anopheles mosquitoes","volume":"111","author":"Hughes","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cub.2021.03.056_bib34","doi-asserted-by":"crossref","first-page":"e36277","DOI":"10.1371\/journal.pone.0036277","article-title":"Invasion of Wolbachia into Anopheles and other insect germlines in an ex\u00a0vivo organ culture system","volume":"7","author":"Hughes","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1016\/j.cub.2021.03.056_bib35","doi-asserted-by":"crossref","first-page":"13847","DOI":"10.1038\/s41598-020-70745-0","article-title":"The Anopheles coluzzii microbiome and its interaction with the intracellular parasite Wolbachia","volume":"10","author":"Straub","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.cub.2021.03.056_bib36","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1098\/rspb.1998.0324","article-title":"Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences","volume":"265","author":"Zhou","year":"1998","journal-title":"Proc. Biol. Sci."},{"key":"10.1016\/j.cub.2021.03.056_bib37","doi-asserted-by":"crossref","first-page":"13155","DOI":"10.1038\/ncomms13155","article-title":"Eukaryotic association module in phage WO genomes from Wolbachia","volume":"7","author":"Bordenstein","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.cub.2021.03.056_bib38","doi-asserted-by":"crossref","first-page":"4987","DOI":"10.1073\/pnas.1800650115","article-title":"One prophage WO gene rescues cytoplasmic incompatibility in Drosophila melanogaster","volume":"115","author":"Shropshire","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cub.2021.03.056_bib39","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1093\/molbev\/msaa209","article-title":"Life and death of selfish genes: comparative genomics reveals the dynamic evolution of cytoplasmic incompatibility","volume":"38","author":"Martinez","year":"2020","journal-title":"Mol. Biol. Evol."},{"issue":"1779","key":"10.1016\/j.cub.2021.03.056_bib40","first-page":"20132837","article-title":"Wolbachia increases susceptibility to Plasmodium infection in a natural system","volume":"281","author":"Z\u00e9l\u00e9","year":"2014","journal-title":"Proc Biol Sci."},{"key":"10.1016\/j.cub.2021.03.056_bib41","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1128\/AEM.06751-11","article-title":"Wolbachia strain wAlbB enhances infection by the rodent malaria parasite Plasmodium berghei in Anopheles gambiae mosquitoes","volume":"78","author":"Hughes","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.cub.2021.03.056_bib42","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1126\/science.1165326","article-title":"Stable introduction of a life-shortening Wolbachia infection into the mosquito Aedes aegypti","volume":"323","author":"McMeniman","year":"2009","journal-title":"Science"},{"key":"10.1016\/j.cub.2021.03.056_bib43","doi-asserted-by":"crossref","first-page":"6963","DOI":"10.1128\/AEM.01038-08","article-title":"Host adaptation of a Wolbachia strain after long-term serial passage in mosquito cell lines","volume":"74","author":"McMeniman","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.cub.2021.03.056_bib44","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1186\/1756-3305-7-336","article-title":"Wolbachia strain wAlbB confers both fitness costs and benefit on Anopheles stephensi","volume":"7","author":"Joshi","year":"2014","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.cub.2021.03.056_bib45","doi-asserted-by":"crossref","first-page":"6814","DOI":"10.1073\/pnas.96.12.6814","article-title":"Four intracellular genomes direct weevil biology: nuclear, mitochondrial, principal endosymbiont, and Wolbachia","volume":"96","author":"Heddi","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cub.2021.03.056_bib46","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.cub.2021.03.056_bib47","doi-asserted-by":"crossref","first-page":"7098","DOI":"10.1128\/AEM.00731-06","article-title":"Multilocus sequence typing system for the endosymbiont Wolbachia pipientis","volume":"72","author":"Baldo","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.cub.2021.03.056_bib48","doi-asserted-by":"crossref","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt removes adapter sequences from high-throughput sequencing reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet.journal"},{"key":"10.1016\/j.cub.2021.03.056_bib49","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: high-resolution sample inference from Illumina amplicon data","volume":"13","author":"Callahan","year":"2016","journal-title":"Nat. Methods"},{"key":"10.1016\/j.cub.2021.03.056_bib50","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1186\/s40168-018-0470-z","article-title":"Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2\u2019s q2-feature-classifier plugin","volume":"6","author":"Bokulich","year":"2018","journal-title":"Microbiome"},{"key":"10.1016\/j.cub.2021.03.056_bib51","doi-asserted-by":"crossref","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA ribosomal RNA gene database project: improved data processing and web-based tools","volume":"41","author":"Quast","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.cub.2021.03.056_bib52","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.cub.2021.03.056_bib53","doi-asserted-by":"crossref","first-page":"D707","DOI":"10.1093\/nar\/gku1117","article-title":"VectorBase: an updated bioinformatics resource for invertebrate vectors and other organisms related with human diseases","volume":"43","author":"Giraldo-Calder\u00f3n","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.cub.2021.03.056_bib54","doi-asserted-by":"crossref","first-page":"1754","DOI":"10.1093\/bioinformatics\/btp324","article-title":"Fast and accurate short read alignment with Burrows-Wheeler transform","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"10.1016\/j.cub.2021.03.056_bib55","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1093\/bioinformatics\/btv033","article-title":"MEGAHIT: an ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph","volume":"31","author":"Li","year":"2015","journal-title":"Bioinformatics"},{"key":"10.1016\/j.cub.2021.03.056_bib56","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1093\/bioinformatics\/btv697","article-title":"MetaQUAST: evaluation of metagenome assemblies","volume":"32","author":"Mikheenko","year":"2016","journal-title":"Bioinformatics"},{"key":"10.1016\/j.cub.2021.03.056_bib57","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1101\/gr.2289704","article-title":"Mauve: multiple alignment of conserved genomic sequence with rearrangements","volume":"14","author":"Darling","year":"2004","journal-title":"Genome Res."},{"key":"10.1016\/j.cub.2021.03.056_bib58","doi-asserted-by":"crossref","first-page":"2071","DOI":"10.1093\/bioinformatics\/btp356","article-title":"Reordering contigs of draft genomes using the Mauve aligner","volume":"25","author":"Rissman","year":"2009","journal-title":"Bioinformatics"},{"key":"10.1016\/j.cub.2021.03.056_bib59","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"The Sequence Alignment\/Map format and SAMtools","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"10.1016\/j.cub.2021.03.056_bib60","doi-asserted-by":"crossref","first-page":"e112963","DOI":"10.1371\/journal.pone.0112963","article-title":"Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement","volume":"9","author":"Walker","year":"2014","journal-title":"PLoS ONE"},{"key":"10.1016\/j.cub.2021.03.056_bib61","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: rapid prokaryotic genome annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.cub.2021.03.056_bib62","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1101\/gr.186072.114","article-title":"CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes","volume":"25","author":"Parks","year":"2015","journal-title":"Genome Res."},{"key":"10.1016\/j.cub.2021.03.056_bib63","doi-asserted-by":"crossref","first-page":"5114","DOI":"10.1038\/s41467-018-07641-9","article-title":"High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries","volume":"9","author":"Jain","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.cub.2021.03.056_bib64","series-title":"Gplots: various R programming tools for plotting data","author":"Warnes","year":"2020"},{"key":"10.1016\/j.cub.2021.03.056_bib65","doi-asserted-by":"crossref","first-page":"2237","DOI":"10.1016\/j.jmb.2017.12.007","article-title":"A completely reimplemented MPI bioinformatics toolkit with a new HHpred server at its core","volume":"430","author":"Zimmermann","year":"2018","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.cub.2021.03.056_bib67","series-title":"ggplot2: Elegant Graphics for Data Analysis","author":"Wickham","year":"2016"},{"key":"10.1016\/j.cub.2021.03.056_bib79","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1186\/1471-2164-12-402","article-title":"BLAST Ring Image Generator (BRIG): simple prokaryote genome comparisons","volume":"12","author":"Alikhan","year":"2011","journal-title":"BMC Genomics"},{"key":"10.1016\/j.cub.2021.03.056_bib66","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1093\/bioinformatics\/btq033","article-title":"BEDTools: a flexible suite of utilities for comparing genomic features","volume":"26","author":"Quinlan","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1016\/j.cub.2021.03.056_bib68","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1186\/s13071-018-3049-4","article-title":"Changes in malaria vector bionomics and transmission patterns in the equatorial forest region of Cameroon between 2000 and 2017","volume":"11","author":"Bamou","year":"2018","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.cub.2021.03.056_bib69","series-title":"The Anophelinae of Africa South of the Sahara","author":"Gillies","year":"1968"},{"key":"10.1016\/j.cub.2021.03.056_bib70","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1093\/trstmh\/try069","article-title":"Identification of Anopheles species in Sud Kivu, Democratic Republic of Congo, using molecular tools","volume":"112","author":"Bandibabone","year":"2018","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.cub.2021.03.056_bib71","doi-asserted-by":"crossref","first-page":"327","DOI":"10.4269\/ajtmh.15-0562","article-title":"Molecular characterization reveals diverse and unknown malaria vectors in the Western Kenyan Highlands","volume":"94","author":"St Laurent","year":"2016","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.cub.2021.03.056_bib72","doi-asserted-by":"crossref","first-page":"478","DOI":"10.4269\/ajtmh.1995.53.478","article-title":"Discrimination of all members of the Anopheles punctulatus complex by polymerase chain reaction--restriction fragment length polymorphism analysis","volume":"53","author":"Beebe","year":"1995","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.cub.2021.03.056_bib73","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1186\/1475-2875-9-161","article-title":"Population genetic structure of the malaria vector Anopheles nili in sub-Saharan Africa","volume":"9","author":"Ndo","year":"2010","journal-title":"Malar. J."},{"key":"10.1016\/j.cub.2021.03.056_bib74","first-page":"512","article-title":"Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees","volume":"10","author":"Tamura","year":"1993","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.cub.2021.03.056_bib75","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1093\/nar\/gks808","article-title":"Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies","volume":"41","author":"Klindworth","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.cub.2021.03.056_bib76","first-page":"2825","article-title":"Scikit-learn: machine learning in Python","volume":"12","author":"Pedregosa","year":"2011","journal-title":"J.\u00a0Mach. Learn. Res."},{"key":"10.1016\/j.cub.2021.03.056_bib77","doi-asserted-by":"crossref","first-page":"e2965","DOI":"10.1371\/journal.pntd.0002965","article-title":"Wolbachia enhances West Nile virus (WNV) infection in the mosquito Culex tarsalis","volume":"8","author":"Dodson","year":"2014","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.cub.2021.03.056_bib78","doi-asserted-by":"crossref","first-page":"e1003381","DOI":"10.1371\/journal.pgen.1003381","article-title":"Comparative genomics of Wolbachia and the bacterial species concept","volume":"9","author":"Ellegaard","year":"2013","journal-title":"PLoS Genet."}],"container-title":["Current Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960982221004292?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960982221004292?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T20:17:08Z","timestamp":1778789828000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960982221004292"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6]]},"references-count":79,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["S0960982221004292"],"URL":"https:\/\/doi.org\/10.1016\/j.cub.2021.03.056","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.10.29.357400","asserted-by":"object"}]},"ISSN":["0960-9822"],"issn-type":[{"value":"0960-9822","type":"print"}],"subject":[],"published":{"date-parts":[[2021,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Stable high-density and maternally inherited Wolbachia infections in Anopheles moucheti and Anopheles demeilloni mosquitoes","name":"articletitle","label":"Article Title"},{"value":"Current Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cub.2021.03.056","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}