{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T08:05:35Z","timestamp":1787731535709,"version":"build-2784847793"},"reference-count":85,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T00:00:00Z","timestamp":1772409600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020082","name":"National Cancer Institute Center for Biomedical Informatics and Information Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020082","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Current Biology"],"published-print":{"date-parts":[[2026,4]]},"DOI":"10.1016\/j.cub.2026.02.065","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T14:55:08Z","timestamp":1774277708000},"page":"1882-1891.e4","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["Big1 is a cell-cycle regulator linking cell size to basal body number in Tetrahymena thermophila"],"prefix":"10.1016","volume":"36","author":[{"given":"Alexander J.","family":"Stemm-Wolf","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam W.J.","family":"Soh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa E.","family":"Mitchell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huangqingbo","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Collet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gayatri L.","family":"Dholakia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vikram","family":"Raju","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jay R.","family":"Hesselberth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Matthew","family":"Taliaferro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert F.","family":"Murphy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia R.","family":"Heasley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1915-6593","authenticated-orcid":false,"given":"Chad G.","family":"Pearson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cub.2026.02.065_bib1","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a019083","article-title":"Cell-Size Control","volume":"8","author":"Amodeo","year":"2016","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib2","doi-asserted-by":"crossref","first-page":"R1414","DOI":"10.1016\/j.cub.2021.09.017","article-title":"Cell-size control","volume":"31","author":"Rhind","year":"2021","journal-title":"Curr. Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib3","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1006548","article-title":"Dilution and titration of cell-cycle regulators may control cell size in budding yeast","volume":"14","author":"Heldt","year":"2018","journal-title":"PLOS Comput. Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib4","doi-asserted-by":"crossref","first-page":"3312","DOI":"10.1016\/j.cub.2023.06.054","article-title":"The fission yeast cell size control system integrates pathways measuring cell surface area, volume, and time","volume":"33","author":"Miller","year":"2023","journal-title":"Curr. Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib5","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.02040","article-title":"Cortical regulation of cell size by a sizer cdr2p","volume":"3","author":"Pan","year":"2014","journal-title":"eLife"},{"key":"10.1016\/j.cub.2026.02.065_bib6","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1083\/jcb.201709171","article-title":"Cell size\u2013dependent regulation of Wee1 localization by Cdr2 cortical nodes","volume":"217","author":"Allard","year":"2018","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib7","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.2003268","article-title":"A map of protein dynamics during cell-cycle progression and cell-cycle exit","volume":"15","author":"Gookin","year":"2017","journal-title":"PLOS Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib8","doi-asserted-by":"crossref","DOI":"10.1186\/gb4184","article-title":"Cyclin-dependent kinases","volume":"15","author":"Malumbres","year":"2014","journal-title":"Genome Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib9","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1091\/mbc.02-02-0030","article-title":"Identification of Genes Periodically Expressed in the Human Cell Cycle and Their Expression in Tumors","volume":"13","author":"Whitfield","year":"2002","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.cub.2026.02.065_bib10","series-title":"Cell Cycle: Regulation by Cyclins. In eLS","author":"Truman","year":"2012"},{"key":"10.1016\/j.cub.2026.02.065_bib11","series-title":"Advances in Protein Chemistry and Structural Biology Control of Cell Cycle & Cell Proliferation","first-page":"125","article-title":"CDK regulators\u2014Cell cycle progression or apoptosis\u2014Scenarios in normal cells and cancerous cells","author":"Nilmani","year":"2023"},{"key":"10.1016\/j.cub.2026.02.065_bib12","first-page":"317","article-title":"Regulation of Cell Cycle Entry and Exit: A Single Cell Perspective","volume":"10","author":"Coller","year":"2019"},{"key":"10.1016\/j.cub.2026.02.065_bib13","doi-asserted-by":"crossref","DOI":"10.3390\/ijms22168544","article-title":"The SCF Complex Is Essential to Maintain Genome and Chromosome Stability","volume":"22","author":"Thompson","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.cub.2026.02.065_bib14","doi-asserted-by":"crossref","DOI":"10.12688\/f1000research.18582.1","article-title":"APC\/C: current understanding and future perspectives","volume":"8","author":"Yamano","year":"2019","journal-title":"F1000Res"},{"key":"10.1016\/j.cub.2026.02.065_bib15","article-title":"The crosstalk between non-coding RNAs and cell-cycle events: A new frontier in cancer therapy","volume":"32","author":"Pathania","year":"2024","journal-title":"Mol. Ther. Oncol."},{"key":"10.1016\/j.cub.2026.02.065_bib16","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1126\/science.aaf7463","article-title":"Cyclin A2 is an RNA binding protein that controls Mre11 mRNA translation","volume":"353","author":"Kanakkanthara","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.cub.2026.02.065_bib17","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1010503","article-title":"Subscaling of a cytosolic RNA binding protein governs cell size homeostasis in the multiple fission alga Chlamydomonas","volume":"20","author":"Liu","year":"2024","journal-title":"PLOS Genet."},{"key":"10.1016\/j.cub.2026.02.065_bib18","doi-asserted-by":"crossref","DOI":"10.1186\/s13008-022-00077-0","article-title":"An updated view on the centrosome as a cell cycle regulator","volume":"17","author":"Lin","year":"2022","journal-title":"Cell Div."},{"key":"10.1016\/j.cub.2026.02.065_bib19","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1038\/ncb918","article-title":"Active cyclin B1\u2013Cdk1 first appears on centrosomes in prophase","volume":"5","author":"Jackman","year":"2003","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib20","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/j.cub.2019.12.053","article-title":"The Hydrophobic Patch Directs Cyclin B to Centrosomes to Promote Global CDK Phosphorylation at Mitosis","volume":"30","author":"Basu","year":"2020","journal-title":"Curr. Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1083\/jcb.115.1.1","article-title":"Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport","volume":"115","author":"Pines","year":"1991","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib22","doi-asserted-by":"crossref","first-page":"3985","DOI":"10.1002\/j.1460-2075.1989.tb08581.x","article-title":"p34cdc2 is located in both nucleus and cytoplasm; part is centrosomally associated at G2\/M and enters vesicles at anaphase","volume":"8","author":"Bailly","year":"1989","journal-title":"EMBO J."},{"key":"10.1016\/j.cub.2026.02.065_bib23","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1126\/science.1103544","article-title":"A Centrosomal Localization Signal in Cyclin E Required for Cdk2-Independent S Phase Entry","volume":"306","author":"Matsumoto","year":"2004","journal-title":"Science"},{"key":"10.1016\/j.cub.2026.02.065_bib24","doi-asserted-by":"crossref","first-page":"8468","DOI":"10.1074\/jbc.M211202200","article-title":"Polo-like Kinase 1 and Chk2 Interact and Co-localize to Centrosomes and the Midbody","volume":"278","author":"Tsvetkov","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.cub.2026.02.065_bib25","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1038\/ncb2633","article-title":"Centrosomal MPF triggers the mitotic and morphogenetic switches of fission yeast","volume":"15","author":"Grallert","year":"2013","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib26","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/S0092-8674(03)00642-1","article-title":"Aurora-A and an Interacting Activator, the LIM Protein Ajuba, Are Required for Mitotic Commitment in Human Cells","volume":"114","author":"Hirota","year":"2003","journal-title":"Cell"},{"key":"10.1016\/j.cub.2026.02.065_bib27","doi-asserted-by":"crossref","first-page":"401","DOI":"10.4161\/cc.7.3.5295","article-title":"Asymmetric localization of the CDC25B phosphatase to the mother centrosome during interphase","volume":"7","author":"Boutros","year":"2008","journal-title":"Cell Cycle"},{"key":"10.1016\/j.cub.2026.02.065_bib28","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1038\/ncb3576","article-title":"Budding yeast Wee1 distinguishes spindle pole bodies to guide their pattern of age-dependent segregation","volume":"19","author":"Lengefeld","year":"2017","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib29","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1091\/mbc.e10-07-0644","article-title":"Spatiotemporal regulations of Wee1 at the G2\/M transition","volume":"22","author":"Masuda","year":"2011","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.cub.2026.02.065_bib30","doi-asserted-by":"crossref","first-page":"2425","DOI":"10.1242\/jcs.108.6.2425","article-title":"Subcellular localisation of human wee1 kinase is regulated during the cell cycle","volume":"108","author":"Baldin","year":"1995","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.cub.2026.02.065_bib31","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/S0167-4889(02)00258-6","article-title":"A cyclin-dependent protein kinase homologue associated with the basal body domains in the ciliate Tetrahymena thermophila","volume":"1591","author":"Zhang","year":"2002","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.cub.2026.02.065_bib32","doi-asserted-by":"crossref","DOI":"10.1091\/mbc.E22-11-0508","article-title":"Basal body organization and cell geometry during the cell cycle in Tetrahymena thermophila","volume":"34","author":"Sun","year":"2023","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.cub.2026.02.065_bib33","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1242\/bio.014951","article-title":"Automated image analysis reveals the dynamic 3-dimensional organization of multi-ciliary arrays","volume":"5","author":"Galati","year":"2015","journal-title":"Biol. Open"},{"key":"10.1016\/j.cub.2026.02.065_bib34","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1002\/jez.1401780204","article-title":"The constancy of cortical units in tetrahymena with varying numbers of ciliary rows","volume":"178","author":"Nanney","year":"1971","journal-title":"J. Exp. Zool."},{"key":"10.1016\/j.cub.2026.02.065_bib35","first-page":"41","article-title":"Mutational analysis of patterning of oral structures in Tetrahymena. I. Effects of increased size on organization","volume":"82","author":"Frankel","year":"1984","journal-title":"J. Embryol. Exp. Morphol."},{"key":"10.1016\/j.cub.2026.02.065_bib36","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1083\/jcb.200908019","article-title":"Basal body stability and ciliogenesis requires the conserved component Poc1","volume":"187","author":"Pearson","year":"2009","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib37","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1083\/jcb.40.3.716","article-title":"The morphogenesis of basal bodies and accessory structures of the cortex of the ciliated protozoan Tetrahymena pyriformis","volume":"40","author":"Allen","year":"1969","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib38","doi-asserted-by":"crossref","DOI":"10.1126\/science.158.3800.527","article-title":"How ciliary Basal bodies develop","volume":"158","author":"Dippell","year":"1967","journal-title":"Science"},{"key":"10.1016\/j.cub.2026.02.065_bib39","doi-asserted-by":"crossref","first-page":"1712","DOI":"10.1128\/EC.00393-07","article-title":"Abnormal Micronuclear Telomeres Lead to an Unusual Cell Cycle Checkpoint and Defects in Tetrahymena Oral Morphogenesis","volume":"7","author":"Kirk","year":"2008","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.cub.2026.02.065_bib40","article-title":"Global and local functions of the Fused kinase ortholog CdaH in intracellular patterning in Tetrahymena","volume":"137","author":"Lee","year":"2023","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.cub.2026.02.065_bib41","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1242\/jcs.43.1.59","article-title":"Temperature-sensitive periods of mutations affecting cell division in Tetrahymena thermophila","volume":"43","author":"Frankel","year":"1980","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.cub.2026.02.065_bib42","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/B978-0-12-385967-9.00007-4","article-title":"Developmental progression of Tetrahymena through the cell cycle and conjugation","volume":"109","author":"Cole","year":"2012","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib43","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/0012-1606(71)90128-X","article-title":"The pattern of replication of cortical units in Tetrahymena","volume":"26","author":"Nanney","year":"1971","journal-title":"Dev. Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib44","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/S0092-8674(04)00247-8","article-title":"Negative Regulation of dE2F1 by Cyclin-Dependent Kinases Controls Cell Cycle Timing","volume":"117","author":"Reis","year":"2004","journal-title":"Cell"},{"key":"10.1016\/j.cub.2026.02.065_bib45","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.1126\/science.8384376","article-title":"Cyclin-dependent regulation of G1 in mammalian fibroblasts","volume":"259","author":"Ohtsubo","year":"1993","journal-title":"Science"},{"key":"10.1016\/j.cub.2026.02.065_bib46","first-page":"1669","article-title":"Acceleration of the G1\/S phase transition by expression of cyclins D1 and E with an inducible system","volume":"14","author":"Resnitzky","year":"1994","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib47","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/0092-8674(86)90546-5","article-title":"cdc25+ functions as an inducer in the mitotic control of fission yeast","volume":"45","author":"Russell","year":"1986","journal-title":"Cell"},{"key":"10.1016\/j.cub.2026.02.065_bib48","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1242\/jcs.34.1.303","article-title":"Nuclear divisions with reduced numbers of microtubules in Tetrahymena","volume":"34","author":"Jaeckel-Williams","year":"1978","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.cub.2026.02.065_bib49","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0091-679X(08)61528-9","article-title":"Cell biology of Tetrahymena thermophila","volume":"62","author":"Frankel","year":"2000","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib50","first-page":"1","article-title":"Mechanism of action of hydroxyurea","volume":"19","author":"Yarbro","year":"1992","journal-title":"Semin. Oncol."},{"key":"10.1016\/j.cub.2026.02.065_bib51","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1005405","article-title":"Checkpoint Activation of an Unconventional DNA Replication Program in Tetrahymena","volume":"11","author":"Sandoval","year":"2015","journal-title":"PLOS Genet."},{"key":"10.1016\/j.cub.2026.02.065_bib52","doi-asserted-by":"crossref","first-page":"2394","DOI":"10.1091\/mbc.e16-03-0165","article-title":"Tetrahymena Poc1 ensures proper intertriplet microtubule linkages to maintain basal body integrity","volume":"27","author":"Meehl","year":"2016","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.cub.2026.02.065_bib53","doi-asserted-by":"crossref","DOI":"10.1083\/jcb.202311104","article-title":"Poc1 bridges basal body inner junctions to promote triplet microtubule integrity and connections","volume":"223","author":"Ruehle","year":"2024","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib54","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1126\/science.213.4507.549","article-title":"Nullisomic tetrahymena: eliminating germinal chromosomes","volume":"213","author":"Bruns","year":"1981","journal-title":"Science"},{"key":"10.1016\/j.cub.2026.02.065_bib55","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/0092-8674(94)90426-X","article-title":"S. pombe mei2+ encodes an RNA-binding protein essential for premeiotic DNA synthesis and meiosis I, which cooperates with a novel RNA species meiRNA","volume":"78","author":"Watanabe","year":"1994","journal-title":"Cell"},{"key":"10.1016\/j.cub.2026.02.065_bib56","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1002\/j.1460-2075.1988.tb02873.x","article-title":"The S.pombe mei2 gene encoding a crucial molecule for commitment to meiosis is under the regulation of cAMP","volume":"7","author":"Watanabe","year":"1988","journal-title":"EMBO J."},{"key":"10.1016\/j.cub.2026.02.065_bib57","doi-asserted-by":"crossref","first-page":"1905","DOI":"10.1105\/tpc.19.00468","article-title":"Control of Grain Size and Weight by the GSK2-LARGE1\/OML4 Pathway in Rice","volume":"32","author":"Lyu","year":"2020","journal-title":"Plant Cell"},{"key":"10.1016\/j.cub.2026.02.065_bib58","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2229-5-2","article-title":"The Arabidopsis Mei2 homologue AML1 binds AtRaptor1B, the plant homologue of a major regulator of eukaryotic cell growth","volume":"5","author":"Anderson","year":"2005","journal-title":"BMC Plant Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib59","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/B978-0-12-385967-9.00013-X","article-title":"Cytological analysis of Tetrahymena thermophila","volume":"109","author":"Winey","year":"2012","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib60","doi-asserted-by":"crossref","first-page":"3734","DOI":"10.1073\/pnas.052016199","article-title":"A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila","volume":"99","author":"Shang","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cub.2026.02.065_bib61","doi-asserted-by":"crossref","DOI":"10.3390\/ijms242115745","article-title":"TOR Complex 1: Orchestrating Nutrient Signaling and Cell Cycle Progression","volume":"24","author":"Foltman","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.cub.2026.02.065_bib62","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1111\/j.1550-7408.1970.tb04708.x","article-title":"Synchronization of the cell cycle of Tetrahymena by stravation and refeeding","volume":"17","author":"Cameron","year":"1970","journal-title":"J. Protozool."},{"key":"10.1016\/j.cub.2026.02.065_bib63","doi-asserted-by":"crossref","DOI":"10.1091\/mbc.E22-08-0326","article-title":"Transcriptome analysis of the binucleate ciliate Tetrahymena thermophila with asynchronous nuclear cell cycles","volume":"34","author":"Zhang","year":"2023","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.cub.2026.02.065_bib64","doi-asserted-by":"crossref","first-page":"3606","DOI":"10.1091\/mbc.e04-10-0919","article-title":"Basal body duplication and maintenance require one member of the Tetrahymena thermophila centrin gene family","volume":"16","author":"Stemm-Wolf","year":"2005","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.cub.2026.02.065_bib65","doi-asserted-by":"crossref","DOI":"10.1083\/jcb.202002077","article-title":"Mutual antagonism between Hippo signaling and cyclin E drives intracellular pattern formation","volume":"219","author":"Jiang","year":"2020","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib66","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1126\/science.283.5403.851","article-title":"Requirement of Cdk2-Cyclin E Activity for Repeated Centrosome Reproduction in Xenopus Egg Extracts","volume":"283","author":"Hinchcliffe","year":"1999","journal-title":"Science"},{"key":"10.1016\/j.cub.2026.02.065_bib67","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1016\/j.cub.2010.03.028","article-title":"Centrosomal Localization of Cyclin E-Cdk2 Is Required for Initiation of DNA Synthesis","volume":"20","author":"Ferguson","year":"2010","journal-title":"Curr. Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib68","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1038\/386187a0","article-title":"Phosphorylation of RNA-binding protein controls cell cycle switch from mitotic to meiotic in fission yeast","volume":"386","author":"Watanabe","year":"1997","journal-title":"Nature"},{"key":"10.1016\/j.cub.2026.02.065_bib69","doi-asserted-by":"crossref","first-page":"2461","DOI":"10.1091\/mbc.e02-11-0738","article-title":"The fission yeast meiotic regulator Mei2p forms a dot structure in the horse-tail nucleus in association with the sme2 locus on chromosome II","volume":"14","author":"Shimada","year":"2003","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.cub.2026.02.065_bib70","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/nature04881","article-title":"Selective elimination of messenger RNA prevents an incidence of untimely meiosis","volume":"442","author":"Harigaya","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.cub.2026.02.065_bib71","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/j.devcel.2007.02.015","article-title":"Centrosomes Promote Timely Mitotic Entry in C. elegans Embryos","volume":"12","author":"Hachet","year":"2007","journal-title":"Dev. Cell"},{"key":"10.1016\/j.cub.2026.02.065_bib72","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1083\/jcb.200503066","article-title":"Cdc25B cooperates with Cdc25A to induce mitosis but has a unique role in activating cyclin B1\u2013Cdk1 at the centrosome","volume":"171","author":"Lindqvist","year":"2005","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib73","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/347680a0","article-title":"Distinct nuclear and spindle pole body populations of cyclin\u2013cdc2 in fission yeast","volume":"347","author":"Alfa","year":"1990","journal-title":"Nature"},{"key":"10.1016\/j.cub.2026.02.065_bib74","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1016\/j.cub.2025.12.051","article-title":"Tetrahymena JanusA gene encodes a polo kinase whose loss triggers a dorsal\/ventral intracellular homeosis","volume":"36","author":"Cole","year":"2026","journal-title":"Curr. Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib82","doi-asserted-by":"crossref","first-page":"1933","DOI":"10.1074\/mcp.M500227-MCP200","article-title":"Fluorescent Proteins as Proteomic Probes","volume":"4","author":"Cristea","year":"2005","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.cub.2026.02.065_bib75","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.gene.2008.08.007","article-title":"High efficiency transformation of Tetrahymena using a codon-optimized neomycin resistance gene","volume":"425","author":"Mochizuki","year":"2008","journal-title":"Gene"},{"key":"10.1016\/j.cub.2026.02.065_bib76","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/S0091-679X(08)61554-X","article-title":"Knockout heterokaryons enable facile mutagenic analysis of essential genes in Tetrahymena","volume":"62","author":"Hai","year":"2000","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib77","first-page":"1659","article-title":"Sfr13, a member of a large family of asymmetrically localized Sfi1-repeat proteins, is important for basal body separation and stability in Tetrahymena thermophila","volume":"126","author":"Stemm-Wolf","year":"2013","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.cub.2026.02.065_bib78","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S0091-679X(08)61530-7","article-title":"Tetrahymena as a laboratory organism: useful strains, cell culture, and cell line maintenance","volume":"62","author":"Orias","year":"2000","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib79","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/S0091-679X(08)61553-8","article-title":"Biolistic transformation of macro- and micronuclei","volume":"62","author":"Bruns","year":"2000","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib80","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/S0091-679X(08)61552-6","article-title":"Transient and stable DNA transformation of Tetrahymena thermophila by electroporation","volume":"62","author":"Gaertig","year":"2000","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib81","doi-asserted-by":"crossref","first-page":"5391","DOI":"10.1093\/nar\/22.24.5391","article-title":"High frequency vector-mediated transformation and gene replacement in Tetrahymena","volume":"22","author":"Gaertig","year":"1994","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.cub.2026.02.065_bib83","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/S0960-9822(00)00490-5","article-title":"The role of pre- and post-anaphase microtubules in the cytokinesis phase of the cell cycle","volume":"10","author":"Canman","year":"2000","journal-title":"Curr. Biol."},{"key":"10.1016\/j.cub.2026.02.065_bib84","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2025.113380","article-title":"OpenEMMU: a versatile, open-source EdU multiplexing methodology for studying DNA replication and cell cycle dynamics","volume":"28","author":"Contreras","year":"2025","journal-title":"iScience"},{"key":"10.1016\/j.cub.2026.02.065_bib85","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"}],"container-title":["Current Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960982226002526?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0960982226002526?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T21:00:13Z","timestamp":1785877213000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0960982226002526"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":85,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["S0960982226002526"],"URL":"https:\/\/doi.org\/10.1016\/j.cub.2026.02.065","relation":{},"ISSN":["0960-9822"],"issn-type":[{"value":"0960-9822","type":"print"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Big1 is a cell-cycle regulator linking cell size to basal body number in Tetrahymena thermophila","name":"articletitle","label":"Article Title"},{"value":"Current Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cub.2026.02.065","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}