{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T04:37:41Z","timestamp":1792643861825,"version":"4.5.0"},"reference-count":34,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cytokine"],"published-print":{"date-parts":[[2021,7]]},"DOI":"10.1016\/j.cyto.2021.155512","type":"journal-article","created":{"date-parts":[[2021,4,3]],"date-time":"2021-04-03T19:50:41Z","timestamp":1617479441000},"page":"155512","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["Puerarin facilitates osteogenesis in steroid-induced necrosis of rabbit femoral head and osteogenesis of steroid-induced osteocytes via miR-34a upregulation"],"prefix":"10.1016","volume":"143","author":[{"given":"Xin","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuguo","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenlong","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wencai","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.cyto.2021.155512_b0005","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1302\/2058-5241.4.180036","article-title":"Osteonecrosis of the femoral head: pathophysiology and current concepts of treatment","volume":"4","author":"Petek","year":"2019","journal-title":"EFORT Open Rev."},{"key":"10.1016\/j.cyto.2021.155512_b0010","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.jot.2018.01.003","article-title":"Steroid-associated osteonecrosis animal model in rats","volume":"13","author":"Zheng","year":"2018","journal-title":"J. Orthopaedic Transl."},{"issue":"3\u20135","key":"10.1016\/j.cyto.2021.155512_b0015","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.jsbmb.2009.02.007","article-title":"Glucocorticoids in osteonecrosis of the femoral head: a new understanding of the mechanisms of action","volume":"114","author":"Kerachian","year":"2009","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"10.1016\/j.cyto.2021.155512_b0020","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.gene.2018.05.091","article-title":"The pathogenesis of steroid-induced osteonecrosis of the femoral head: a systematic review of the literature","volume":"671","author":"Wang","year":"2018","journal-title":"Gene"},{"issue":"2","key":"10.1016\/j.cyto.2021.155512_b0025","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/s12020-011-9580-0","article-title":"Glucocorticoid-induced osteonecrosis","volume":"41","author":"Weinstein","year":"2012","journal-title":"Endocrine"},{"issue":"Suppl 3","key":"10.1016\/j.cyto.2021.155512_b0030","doi-asserted-by":"crossref","first-page":"S131","DOI":"10.2215\/CJN.04151206","article-title":"Normal bone anatomy and physiology","volume":"3","author":"Clarke","year":"2008","journal-title":"Clin. J. Am. Soc. Nephrol: CJASN"},{"issue":"2","key":"10.1016\/j.cyto.2021.155512_b0035","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1080\/03008207.2017.1290085","article-title":"Osteoblast-osteoclast interactions","volume":"59","author":"Chen","year":"2018","journal-title":"Connect. Tissue Res."},{"issue":"19","key":"10.1016\/j.cyto.2021.155512_b0040","doi-asserted-by":"crossref","DOI":"10.3390\/ijms20194925","article-title":"From Osteoclast differentiation to osteonecrosis of the jaw: molecular and clinical insights","volume":"20","author":"Anesi","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.cyto.2021.155512_b0045","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.ejphar.2015.03.028","article-title":"Animal models for osteoporosis","volume":"759","author":"Komori","year":"2015","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.cyto.2021.155512_b0050","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.cbi.2019.03.008","article-title":"Naringin regulates bone metabolism in glucocorticoid-induced osteonecrosis of the femoral head via the Akt\/Bad signal cascades","volume":"304","author":"Kuang","year":"2019","journal-title":"Chem. Biol. Interact."},{"issue":"7","key":"10.1016\/j.cyto.2021.155512_b0055","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1002\/ptr.5083","article-title":"Puerarin: a review of pharmacological effects","volume":"28","author":"Zhou","year":"2014","journal-title":"Phytother. Res.: PTR"},{"issue":"7","key":"10.1016\/j.cyto.2021.155512_b0060","doi-asserted-by":"crossref","first-page":"488","DOI":"10.2174\/1566524018666171219101142","article-title":"Puerarin stimulates osteogenic differentiation and bone formation through the ERK1\/2 and p38-MAPK signaling pathways","volume":"17","author":"Yang","year":"2018","journal-title":"Curr. Mol. Med."},{"issue":"11","key":"10.1016\/j.cyto.2021.155512_b0065","doi-asserted-by":"crossref","first-page":"2055","DOI":"10.1002\/art.1780401119","article-title":"Effects of pulse methylprednisolone on bone and marrow tissues: corticosteroid-induced osteonecrosis in rabbits","volume":"40","author":"Yamamoto","year":"1997","journal-title":"Arthritis Rheum."},{"issue":"4","key":"10.1016\/j.cyto.2021.155512_b0070","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1016\/j.bone.2006.04.018","article-title":"Multiple bioimaging modalities in evaluation of an experimental osteonecrosis induced by a combination of lipopolysaccharide and methylprednisolone","volume":"39","author":"Qin","year":"2006","journal-title":"Bone"},{"issue":"4","key":"10.1016\/j.cyto.2021.155512_b0075","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods (San Diego, Calif.)"},{"issue":"7","key":"10.1016\/j.cyto.2021.155512_b0080","doi-asserted-by":"crossref","DOI":"10.3390\/ijms20071694","article-title":"Regulation of proliferation, differentiation and functions of osteoblasts by Runx 2","volume":"20","author":"Komori","year":"2019","journal-title":"Int. J. Mol. Sci."},{"issue":"Suppl 3","key":"10.1016\/j.cyto.2021.155512_b0085","first-page":"148","article-title":"Alcohol-induced adipogenesis in a cloned bone-marrow stem cell","volume":"88","author":"Cui","year":"2006","journal-title":"J. Bone Joint Surg. Am."},{"issue":"1","key":"10.1016\/j.cyto.2021.155512_b0090","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1002\/jor.23613","article-title":"Regulatory effect of microRNA-34a on osteogenesis and angiogenesis in glucocorticoid-induced osteonecrosis of the femoral head","volume":"36","author":"Zha","year":"2018","journal-title":"J. Orthopaedic Res.: Off. Publ. Orthopaedic Res. Soc."},{"issue":"1","key":"10.1016\/j.cyto.2021.155512_b0095","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1186\/s13287-019-1498-0","article-title":"The shift in the balance between osteoblastogenesis and adipogenesis of mesenchymal stem cells mediated by glucocorticoid receptor","volume":"10","author":"Han","year":"2019","journal-title":"Stem Cell Res. Ther."},{"issue":"4","key":"10.1016\/j.cyto.2021.155512_b0100","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1007\/s13239-019-00435-0","article-title":"Cilostazol promotes angiogenesis and increases cell proliferation after myocardial ischemia-reperfusion injury through a cAMP-dependent mechanism","volume":"10","author":"Li","year":"2019","journal-title":"Cardiovasc. Eng. Technol."},{"issue":"2","key":"10.1016\/j.cyto.2021.155512_b0105","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1152\/physrev.00011.2015","article-title":"Molecular actions of glucocorticoids in cartilage and bone during health disease, and steroid therapy","volume":"96","author":"Hartmann","year":"2016","journal-title":"Physiol. Rev."},{"issue":"10","key":"10.1016\/j.cyto.2021.155512_b0110","doi-asserted-by":"crossref","DOI":"10.1038\/cddis.2013.348","article-title":"Dexamethasone shifts bone marrow stromal cells from osteoblasts to adipocytes by C\/EBPalpha promoter methylation","volume":"4","author":"Li","year":"2013","journal-title":"Cell Death Dis."},{"issue":"6","key":"10.1016\/j.cyto.2021.155512_b0115","doi-asserted-by":"crossref","first-page":"2139","DOI":"10.1083\/jcb.106.6.2139","article-title":"Differentiation of muscle, fat, cartilage, and bone from progenitor cells present in a bone-derived clonal cell population: effect of dexamethasone","volume":"106","author":"Grigoriadis","year":"1988","journal-title":"J. Cell Biol."},{"issue":"2","key":"10.1016\/j.cyto.2021.155512_b0120","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1159\/000490023","article-title":"Icariin protects against glucocorticoid-induced osteonecrosis of the femoral head in rats","volume":"47","author":"Huang","year":"2018","journal-title":"Cell. Physiol. Biochem.: Int. J. Exp. Cell. Physiol., Biochem., Pharmacol."},{"issue":"4","key":"10.1016\/j.cyto.2021.155512_b0125","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1002\/1097-0177(2000)9999:9999<::AID-DVDY1074>3.0.CO;2-C","article-title":"Cbfa1: a molecular switch in osteoblast biology","volume":"219","author":"Ducy","year":"2000","journal-title":"Dev. Dyn.: Off. Publ. Am. Assoc. Anat."},{"key":"10.1016\/j.cyto.2021.155512_b0130","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.lfs.2018.06.006","article-title":"Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway","volume":"207","author":"Fan","year":"2018","journal-title":"Life Sci."},{"issue":"6","key":"10.1016\/j.cyto.2021.155512_b0135","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1007\/s00198-003-1572-3","article-title":"Evidence supporting a role of glucocorticoids in short-term bone loss in burned children","volume":"15","author":"Klein","year":"2004","journal-title":"Osteoporosis Int.: J. Established Result Cooperation European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA"},{"key":"10.1016\/j.cyto.2021.155512_b0140","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.lfs.2016.09.024","article-title":"Coadministration of puerarin (low dose) and zinc attenuates bone loss and suppresses bone marrow adiposity in ovariectomized rats","volume":"166","author":"Liu","year":"2016","journal-title":"Life Sci."},{"issue":"5","key":"10.1016\/j.cyto.2021.155512_b0145","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1007\/s11999-008-0178-7","article-title":"Preventive effects of puerarin on alcohol-induced osteonecrosis","volume":"466","author":"Wang","year":"2008","journal-title":"Clin. Orthop. Relat. Res."},{"issue":"12","key":"10.1016\/j.cyto.2021.155512_b0150","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.1016\/j.phymed.2014.06.019","article-title":"Pueraria mirifica extract and puerarin enhance proliferation and expression of alkaline phosphatase and type I collagen in primary baboon osteoblasts","volume":"21","author":"Tiyasatkulkovit","year":"2014","journal-title":"Phytomed.: Int. J. Phytother. Phytopharmacol."},{"issue":"5","key":"10.1016\/j.cyto.2021.155512_b0155","doi-asserted-by":"crossref","first-page":"6262","DOI":"10.3892\/mmr.2017.7386","article-title":"Puerarin promotes the viability and differentiation of MC3T3-E1 cells by miR-204-regulated Runx2 upregulation","volume":"16","author":"Zhan","year":"2017","journal-title":"Mol. Med. Rep."},{"issue":"6","key":"10.1016\/j.cyto.2021.155512_b0160","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1002\/ptr.6034","article-title":"Puerarin inhibits TRPM3\/miR-204 to promote MC3T3-E1 cells proliferation, differentiation and mineralization","volume":"32","author":"Zeng","year":"2018","journal-title":"Phytother. Res.: PTR"},{"issue":"10","key":"10.1016\/j.cyto.2021.155512_b0165","doi-asserted-by":"crossref","DOI":"10.3390\/ijms18102089","article-title":"MicroRNA-34a: a versatile regulator of myriads of targets in different cancers","volume":"18","author":"Farooqi","year":"2017","journal-title":"Int. J. Mol. Sci."},{"issue":"12","key":"10.1016\/j.cyto.2021.155512_b0170","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1177\/1535370217703975","article-title":"MicroRNA-34a alleviates steroid-induced avascular necrosis of femoral head by targeting Tgif2 through OPG\/RANK\/RANKL signaling pathway","volume":"242","author":"Peng","year":"2017","journal-title":"Exp. Biol. Med. (Maywood N.J.)"}],"container-title":["Cytokine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1043466621000922?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1043466621000922?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T21:01:05Z","timestamp":1760907665000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1043466621000922"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7]]},"references-count":34,"alternative-id":["S1043466621000922"],"URL":"https:\/\/doi.org\/10.1016\/j.cyto.2021.155512","relation":{},"ISSN":["1043-4666"],"issn-type":[{"value":"1043-4666","type":"print"}],"subject":[],"published":{"date-parts":[[2021,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Puerarin facilitates osteogenesis in steroid-induced necrosis of rabbit femoral head and osteogenesis of steroid-induced osteocytes via miR-34a upregulation","name":"articletitle","label":"Article Title"},{"value":"Cytokine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cyto.2021.155512","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"155512"}}