{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T18:02:15Z","timestamp":1791828135405,"version":"4.3.4"},"reference-count":57,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2009,5,1]],"date-time":"2009-05-01T00:00:00Z","timestamp":1241136000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental &amp; Comparative Immunology"],"published-print":{"date-parts":[[2009,5]]},"DOI":"10.1016\/j.dci.2008.11.013","type":"journal-article","created":{"date-parts":[[2008,12,26]],"date-time":"2008-12-26T04:17:26Z","timestamp":1230265046000},"page":"681-689","source":"Crossref","is-referenced-by-count":152,"title":["A serpin from the parasitoid wasp Leptopilina boulardi targets the Drosophila phenoloxidase cascade"],"prefix":"10.1016","volume":"33","author":[{"given":"Dominique","family":"Colinet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurore","family":"Dubuffet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Cazes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Moreau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Michel","family":"Drezen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryl\u00e8ne","family":"Poiri\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.dci.2008.11.013_bib1","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1002\/bies.1049","article-title":"Innate immunity and its evasion and suppression by hymenopteran endoparasitoids","volume":"23","author":"Schmidt","year":"2001","journal-title":"Bioessays"},{"key":"10.1016\/j.dci.2008.11.013_bib2","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/j.1744-7917.2008.00188.x","article-title":"Insect immune resistance to parasitoids","volume":"15","author":"Carton","year":"2008","journal-title":"Insect Science"},{"key":"10.1016\/j.dci.2008.11.013_bib3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1744-7917.2008.00183.x","article-title":"The insect cellular immune response","volume":"15","author":"Strand","year":"2008","journal-title":"Insect Sci"},{"issue":"8","key":"10.1016\/j.dci.2008.11.013_bib4","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1111\/j.1365-2443.2005.00883.x","article-title":"Drosophila melanogaster Rac2 is necessary for a proper cellular immune response","volume":"10","author":"Williams","year":"2005","journal-title":"Genes Cells"},{"key":"10.1016\/j.dci.2008.11.013_bib5","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1111\/j.0105-2896.2004.00116.x","article-title":"The prophenoloxidase-activating system in invertebrates","volume":"198","author":"Cerenius","year":"2004","journal-title":"Immunol Rev"},{"issue":"5","key":"10.1016\/j.dci.2008.11.013_bib6","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.ibmb.2005.01.014","article-title":"Melanogenesis and associated cytotoxic reactions: applications to insect innate immunity","volume":"35","author":"Nappi","year":"2005","journal-title":"Insect Biochem Mol Biol"},{"issue":"6","key":"10.1016\/j.dci.2008.11.013_bib7","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.it.2008.02.009","article-title":"The proPO-system: pros and cons for its role in invertebrate immunity","volume":"29","author":"Cerenius","year":"2008","journal-title":"Trends Immun"},{"key":"10.1016\/j.dci.2008.11.013_bib8","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1146\/annurev.ento.51.110104.151029","article-title":"Evolution of developmental strategies in parasitic hymenoptera","volume":"51","author":"Pennacchio","year":"2006","journal-title":"Annu Rev Entomol"},{"issue":"3","key":"10.1016\/j.dci.2008.11.013_bib9","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0020-1790(90)90046-W","article-title":"Host hemolymph monophenoloxidase activity in parasited Manduca sexta larvae and evidence for inhibition by wasp polydnavirus","volume":"20","author":"Beckage","year":"1990","journal-title":"Insect Biochem"},{"issue":"9","key":"10.1016\/j.dci.2008.11.013_bib10","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1016\/S0022-1910(00)00048-2","article-title":"Evidence for serine protease inhibitor activity in the ovarian calyx fluid of the endoparasitoid Venturia canescens","volume":"46","author":"Beck","year":"2000","journal-title":"J Insect Physiol"},{"issue":"2","key":"10.1016\/j.dci.2008.11.013_bib11","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1006\/jipa.2000.4956","article-title":"Intraspecific variation in the effects of parasitism by Asobara tabida on phenoloxidase activity of Drosophila melanogaster larvae","volume":"76","author":"Moreau","year":"2000","journal-title":"J Invertebr Pathol"},{"issue":"3","key":"10.1016\/j.dci.2008.11.013_bib12","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/S0305-0491(00)00255-8","article-title":"Parasitism of Lacanobia oleracea (Lepidoptera) by the ectoparasitoid, Eulophus pennicornis, is associated with a reduction in host haemolymph phenoloxidase activity","volume":"127","author":"Richards","year":"2000","journal-title":"Comp Biochem Physiol B Biochem Mol Biol"},{"issue":"3","key":"10.1016\/j.dci.2008.11.013_bib13","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1006\/jipa.2000.4925","article-title":"Parasitism-linked block of host plasma melanization","volume":"75","author":"Shelby","year":"2000","journal-title":"J Invertebr Pathol"},{"issue":"10","key":"10.1016\/j.dci.2008.11.013_bib14","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1016\/S0965-1748(03)00116-4","article-title":"A serine proteinase homolog venom protein from an endoparasitoid wasp inhibits melanization of the host hemolymph","volume":"33","author":"Asgari","year":"2003","journal-title":"Insect Biochem Mol Biol"},{"issue":"4","key":"10.1016\/j.dci.2008.11.013_bib15","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S0145-305X(02)00101-5","article-title":"Comparative study of the strategies evolved by two parasitoids of the genus Asobara to avoid the immune response of the host Drosophila melanogaster","volume":"27","author":"Moreau","year":"2003","journal-title":"Dev Comp Immunol"},{"issue":"49","key":"10.1016\/j.dci.2008.11.013_bib16","doi-asserted-by":"crossref","first-page":"19267","DOI":"10.1073\/pnas.0708056104","article-title":"A novel polydnavirus protein inhibits the insect prophenoloxidase activation pathway","volume":"104","author":"Beck","year":"2007","journal-title":"Proc Natl Acad Sci USA"},{"issue":"5","key":"10.1016\/j.dci.2008.11.013_bib17","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.ibmb.2004.02.009","article-title":"Negative regulation of prophenoloxidase (proPO) activation by a clip-domain serine proteinase homolog (SPH) from endoparasitoid venom","volume":"34","author":"Zhang","year":"2004","journal-title":"Insect Biochem Mol Biol"},{"key":"10.1016\/j.dci.2008.11.013_bib18","series-title":"The genetics and biology of Drosophila","first-page":"347","article-title":"The Drosophila parasitic wasps","author":"Carton","year":"1986"},{"issue":"2","key":"10.1016\/j.dci.2008.11.013_bib19","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.ibmb.2004.10.004","article-title":"A RhoGAP protein as a main immune suppressive factor in the Leptopilina boulardi (Hymenoptera Figitidae)-Drosophila melanogaster interaction","volume":"35","author":"Labrosse","year":"2005","journal-title":"Insect Biochem Mol Biol"},{"issue":"2","key":"10.1016\/j.dci.2008.11.013_bib20","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.jinsphys.2004.10.004","article-title":"Haemocyte changes in D. melanogaster in response to long gland components of the parasitoid wasp Leptopilina boulardi: a Rho-GAP protein as an important factor","volume":"51","author":"Labrosse","year":"2005","journal-title":"J Insect Physiol"},{"issue":"12","key":"10.1016\/j.dci.2008.11.013_bib21","doi-asserted-by":"crossref","first-page":"e203","DOI":"10.1371\/journal.ppat.0030203","article-title":"Convergent use of RhoGAP toxins by eukaryotic parasites and bacterial pathogens","volume":"3","author":"Colinet","year":"2007","journal-title":"PLoS Pathog"},{"issue":"1","key":"10.1016\/j.dci.2008.11.013_bib22","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/sj.hdy.6800893","article-title":"Genetic interactions between the parasitoid wasp Leptopilina boulardi and its Drosophila hosts","volume":"98","author":"Dubuffet","year":"2007","journal-title":"Heredity"},{"key":"10.1016\/j.dci.2008.11.013_bib23","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/j.dci.2007.10.009","article-title":"Variation of success of Leptopilina boulardi in Drosophila yakuba: the mechanisms explored","volume":"32","author":"Dubuffet","year":"2008","journal-title":"Dev Comp Immunol"},{"issue":"8","key":"10.1016\/j.dci.2008.11.013_bib24","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1002\/1615-9861(200108)1:8<946::AID-PROT946>3.0.CO;2-P","article-title":"Analysis of protein sequences and protein complexes by matrix-assisted laser desorption\/ionization mass spectrometry","volume":"1","author":"Belghazi","year":"2001","journal-title":"Proteomics"},{"issue":"5","key":"10.1016\/j.dci.2008.11.013_bib25","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"MUSCLE: multiple sequence alignment with high accuracy and high throughput","volume":"32","author":"Edgar","year":"2004","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"10.1016\/j.dci.2008.11.013_bib26","doi-asserted-by":"crossref","first-page":"W465","DOI":"10.1093\/nar\/gkn180","article-title":"Phylogeny.fr: robust phylogenetic analysis for the non-specialist","volume":"36","author":"Dereeper","year":"2008","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"10.1016\/j.dci.2008.11.013_bib27","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1093\/bioinformatics\/bti770","article-title":"The SWISS-MODEL Workspace: a web-based environment for protein structure homology modelling","volume":"22","author":"Arnold","year":"2006","journal-title":"Bioinformatics"},{"issue":"18","key":"10.1016\/j.dci.2008.11.013_bib28","doi-asserted-by":"crossref","first-page":"13759","DOI":"10.1074\/jbc.M701074200","article-title":"Structure of native protein C inhibitor provides insight into its multiple functions","volume":"282","author":"Li","year":"2007","journal-title":"J Biol Chem"},{"issue":"6580","key":"10.1016\/j.dci.2008.11.013_bib29","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1038\/381272a0","article-title":"Errors in protein structures","volume":"381","author":"Hooft","year":"1996","journal-title":"Nature"},{"key":"10.1016\/j.dci.2008.11.013_bib30","doi-asserted-by":"crossref","first-page":"D266","DOI":"10.1093\/nar\/gki001","article-title":"PDBsum more: new summaries and analyses of the known 3D structures of proteins and nucleic acids","volume":"33","author":"Laskowski","year":"2005","journal-title":"Nucleic Acids Res"},{"issue":"12","key":"10.1016\/j.dci.2008.11.013_bib31","doi-asserted-by":"crossref","first-page":"2577","DOI":"10.1002\/bip.360221211","article-title":"Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features","volume":"22","author":"Kabsch","year":"1983","journal-title":"Biopolymers"},{"key":"10.1016\/j.dci.2008.11.013_bib32","doi-asserted-by":"crossref","first-page":"4751","DOI":"10.1021\/cr010170+","article-title":"Serpin structure, mechanism, and function","volume":"102","author":"Gettins","year":"2002","journal-title":"Chem Rev"},{"issue":"2","key":"10.1016\/j.dci.2008.11.013_bib33","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.1074\/jbc.272.2.1082","article-title":"Characterization and functional analysis of 12 naturally occurring reactive site variants of serpin-1 from Manduca sexta","volume":"272","author":"Jiang","year":"1997","journal-title":"J Biol Chem"},{"issue":"7","key":"10.1016\/j.dci.2008.11.013_bib34","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/0022-1910(90)90104-N","article-title":"Encapsulation of parasitoid eggs in phenoloxidase-deficient mutants of Drosophila melanogaster","volume":"36","author":"Rizki","year":"1990","journal-title":"J Insect Physiol"},{"issue":"2","key":"10.1016\/j.dci.2008.11.013_bib35","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1038\/sj.embor.7400592","article-title":"Prophenoloxidase activation is not required for survival to microbial infections in Drosophila","volume":"7","author":"Leclerc","year":"2006","journal-title":"EMBO Rep"},{"issue":"11","key":"10.1016\/j.dci.2008.11.013_bib36","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1016\/0022-1910(91)90080-J","article-title":"Alterations in the haemocytes of Pseudoplusia includens after parasitism by Microplitis demolitor","volume":"37","author":"Strand","year":"1991","journal-title":"J Insect Physiol"},{"issue":"5","key":"10.1016\/j.dci.2008.11.013_bib37","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/S0022-1910(03)00054-4","article-title":"Active suppression of D. melanogaster immune response by long gland products of the parasitic wasp Leptopilina boulardi","volume":"49","author":"Labrosse","year":"2003","journal-title":"J Insect Physiol"},{"issue":"2","key":"10.1016\/j.dci.2008.11.013_bib38","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1002\/arch.20199","article-title":"Parasite suppression of the oxidations of eumelanin precursors in Drosophila melanogaster","volume":"66","author":"Kohler","year":"2007","journal-title":"Arch Insect Biochem Physiol"},{"key":"10.1016\/j.dci.2008.11.013_bib39","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1111\/j.0105-2896.2004.0121.x","article-title":"Innate immune responses of a lepidopteran insect, Manduca sexta","volume":"198","author":"Kanost","year":"2004","journal-title":"Immunol Rev"},{"issue":"2","key":"10.1016\/j.dci.2008.11.013_bib40","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1007\/s10059-000-0186-2","article-title":"Immunological detection of serpin in the fall webworm. Hyphantria cunea and its inhibitory activity on the prophenoloxidase system","volume":"10","author":"Park","year":"2000","journal-title":"Mol Cells"},{"issue":"4","key":"10.1016\/j.dci.2008.11.013_bib41","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/S1534-5807(02)00267-8","article-title":"An immune-responsive serpin regulates the melanization cascade in Drosophila","volume":"3","author":"De Gregorio","year":"2002","journal-title":"Dev Cell"},{"issue":"10","key":"10.1016\/j.dci.2008.11.013_bib42","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1016\/S0965-1748(03)00123-1","article-title":"Prophenoloxidase-activating proteinase-3 (pap-3) from Manduca sexta hemolymph: a clip-domain serine proteinase regulated by serpin-1J and serine proteinase homologs","volume":"33","author":"Jiang","year":"2003","journal-title":"Insect Biochem Mol Biol"},{"issue":"47","key":"10.1016\/j.dci.2008.11.013_bib43","doi-asserted-by":"crossref","first-page":"46556","DOI":"10.1074\/jbc.M309682200","article-title":"Manduca sexta serpin-3 regulates prophenoloxidase activation in response to infection by inhibiting prophenoloxidase-activating proteinases","volume":"278","author":"Zhu","year":"2003","journal-title":"J Biol Chem"},{"issue":"15","key":"10.1016\/j.dci.2008.11.013_bib44","doi-asserted-by":"crossref","first-page":"14923","DOI":"10.1074\/jbc.M500531200","article-title":"Manduca sexta serpin-4 and serpin-5 inhibit the prophenol oxidase activation pathway: cDNA cloning, protein expression, and characterization","volume":"280","author":"Tong","year":"2005","journal-title":"J Biol Chem"},{"issue":"14","key":"10.1016\/j.dci.2008.11.013_bib45","doi-asserted-by":"crossref","first-page":"14341","DOI":"10.1074\/jbc.M500570200","article-title":"Manduca sexta serpin-6 regulates immune serine proteinases PAP-3 and HP8. cdna cloning, protein expression, inhibition kinetics, and function elucidation","volume":"280","author":"Zou","year":"2005","journal-title":"J Biol Chem"},{"issue":"45","key":"10.1016\/j.dci.2008.11.013_bib46","doi-asserted-by":"crossref","first-page":"16858","DOI":"10.1073\/pnas.0608033103","article-title":"Increased melanizing activity in Anopheles gambiae does not affect development of Plasmodium falciparum","volume":"103","author":"Michel","year":"2006","journal-title":"Proc Natl Acad Sci USA"},{"issue":"2","key":"10.1016\/j.dci.2008.11.013_bib47","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.ydbio.2008.08.030","article-title":"Drosophila Serpin-28D regulates hemolymph phenoloxidase activity and adult pigmentation","volume":"323","author":"Scherfer","year":"2008","journal-title":"Dev Biol"},{"issue":"23","key":"10.1016\/j.dci.2008.11.013_bib48","doi-asserted-by":"crossref","first-page":"6330","DOI":"10.1093\/emboj\/cdf661","article-title":"A serpin mutant links toll activation to melanization in the host defence of Drosophila","volume":"21","author":"Ligoxygakis","year":"2002","journal-title":"EMBO J"},{"issue":"2","key":"10.1016\/j.dci.2008.11.013_bib49","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.jinsphys.2004.10.013","article-title":"Drosophila serpin 27A is a likely target for immune suppression of the blood cell-mediated melanotic encapsulation response","volume":"51","author":"Nappi","year":"2005","journal-title":"J Insect Physiol"},{"issue":"11","key":"10.1016\/j.dci.2008.11.013_bib50","doi-asserted-by":"crossref","first-page":"R94","DOI":"10.1186\/gb-2005-6-11-r94","article-title":"Genome-wide gene expression in response to parasitoid attack in Drosophila","volume":"6","author":"Wertheim","year":"2005","journal-title":"Genome Biol"},{"issue":"10","key":"10.1016\/j.dci.2008.11.013_bib51","doi-asserted-by":"crossref","first-page":"1486","DOI":"10.1371\/journal.ppat.0030158","article-title":"Contrasting infection strategies in generalist and specialist wasp parasitoids of Drosophila melanogaster","volume":"3","author":"Schlenke","year":"2007","journal-title":"PLoS Pathog"},{"issue":"12","key":"10.1016\/j.dci.2008.11.013_bib52","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1016\/j.ibmb.2005.08.007","article-title":"Prophenoloxidase binds to the surface of hemocytes and is involved in hemocyte melanization in Manduca sexta","volume":"35","author":"Ling","year":"2005","journal-title":"Insect Biochem Mol Biol"},{"issue":"3","key":"10.1016\/j.dci.2008.11.013_bib53","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0968-0004(00)01761-8","article-title":"Serine proteinase inhibitors from nematodes and the arms race between host and pathogen","volume":"26","author":"Zang","year":"2001","journal-title":"Trends Biochem Sci"},{"issue":"2","key":"10.1016\/j.dci.2008.11.013_bib54","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1111\/j.1365-3024.2006.00913.x","article-title":"Proteinase inhibitors and helminth parasite infection","volume":"29","author":"Knox","year":"2007","journal-title":"Parasite Immunol"},{"issue":"1","key":"10.1016\/j.dci.2008.11.013_bib55","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1017\/S0031182000065173","article-title":"Insect immunity: early events in the encapsulation process of parasitoid (Leptopilina boulardi) eggs in resistant and susceptible strains of Drosophila","volume":"112","author":"Russo","year":"1996","journal-title":"Parasitology"},{"issue":"4","key":"10.1016\/j.dci.2008.11.013_bib56","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.ibmb.2003.12.005","article-title":"Purification and characterization of Manduca sexta serpin-6: a serine proteinase inhibitor that selectively inhibits prophenoloxidase-activating proteinase-3","volume":"34","author":"Wang","year":"2004","journal-title":"Insect Biochem Mol Biol"},{"issue":"7","key":"10.1016\/j.dci.2008.11.013_bib57","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1110\/ps.9.7.1274","article-title":"Topography of a 2.0 A structure of alpha1-antitrypsin reveals targets for rational drug design to prevent conformational disease","volume":"9","author":"Elliott","year":"2000","journal-title":"Protein Sci"}],"container-title":["Developmental &amp; Comparative Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0145305X08002759?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0145305X08002759?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,12,23]],"date-time":"2018-12-23T05:26:01Z","timestamp":1545542761000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0145305X08002759"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,5]]},"references-count":57,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2009,5]]}},"alternative-id":["S0145305X08002759"],"URL":"https:\/\/doi.org\/10.1016\/j.dci.2008.11.013","relation":{},"ISSN":["0145-305X"],"issn-type":[{"value":"0145-305X","type":"print"}],"subject":[],"published":{"date-parts":[[2009,5]]}}}