{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:27:20Z","timestamp":1791592040367,"version":"4.3.4"},"reference-count":51,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental & Comparative Immunology"],"published-print":{"date-parts":[[2010,11]]},"DOI":"10.1016\/j.dci.2010.06.009","type":"journal-article","created":{"date-parts":[[2010,7,2]],"date-time":"2010-07-02T08:23:20Z","timestamp":1278059000000},"page":"1175-1182","source":"Crossref","is-referenced-by-count":87,"title":["Enterotoxigenic Escherichia coli (K88) induce proinflammatory responses in porcine intestinal epithelial cells"],"prefix":"10.1016","volume":"34","author":[{"given":"Bert","family":"Devriendt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edith","family":"Stuyven","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Verdonck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno M.","family":"Goddeeris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Cox","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.dci.2010.06.009_bib1","article-title":"Innate immune activation of swine intestinal epithelial cell lines (IPEC-J2 and IPI-2I) in response to LPS from Salmonella typhimurium","author":"Arce","year":"2008","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"issue":"2","key":"10.1016\/j.dci.2010.06.009_bib2","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1111\/j.1365-2958.1992.tb02006.x","article-title":"Characterization of the antigenic and adhesive properties of FaeG, the major subunit of K88 fimbriae","volume":"6","author":"Bakker","year":"1992","journal-title":"Mol. Microbiol."},{"issue":"41","key":"10.1016\/j.dci.2010.06.009_bib3","doi-asserted-by":"crossref","first-page":"42984","DOI":"10.1074\/jbc.M405410200","article-title":"In vitro and ex vivo activation of the TLR5 signaling pathway in intestinal epithelial cells by a commensal Escherichia coli strain","volume":"279","author":"Bambou","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.dci.2010.06.009_bib4","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/j.micpath.2008.01.004","article-title":"IL-1\u03b2 expression in Int407 is induced by flagellin of Vibrio cholerae through TLR5 mediated pathway","volume":"44","author":"Bandyopadhaya","year":"2008","journal-title":"Microb. Pathogenesis"},{"key":"10.1016\/j.dci.2010.06.009_bib5","first-page":"A41","article-title":"Development of normal cultured small intestinal epithelial cell line which transports Na and Cl","volume":"96","author":"Berschneider","year":"1989","journal-title":"Gastroenterology"},{"issue":"3\u20134","key":"10.1016\/j.dci.2010.06.009_bib6","first-page":"309","article-title":"Expression of Toll-like receptors, interleukin 8, macrophage migration inhibitory factor, and osteopontin in tissues from pigs challenged with Salmonella enterica serovar Typhimurium or serovar Choleraesuis","volume":"115","author":"Burkey","year":"2006","journal-title":"Vet. Immunol. Immunopathol."},{"issue":"12","key":"10.1016\/j.dci.2010.06.009_bib7","doi-asserted-by":"crossref","first-page":"7010","DOI":"10.1128\/IAI.68.12.7010-7017.2000","article-title":"Differential alteration in intestinal epithelial cell expression of Toll-like receptor 3 (TLR3) and TLR4 in inflammatory bowel disease","volume":"68","author":"Cario","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.dci.2010.06.009_bib8","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.vetmic.2004.06.014","article-title":"Prevalence of serogroups and virulence factors of Escherichia coli strains isolated from pigs with postweaning diarrhoea in eastern China","volume":"103","author":"Chen","year":"2004","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.dci.2010.06.009_bib9","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0732-8893(01)00303-0","article-title":"Diarrhoeagenic Escherichia coli\u2014an emerging problem?","volume":"41","author":"Clarke","year":"2001","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.dci.2010.06.009_bib10","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/S0165-2427(02)00054-5","article-title":"Oral immunization of pigs with fimbrial antigens from enterotoxigenic E. coli: an interesting model to study mucosal immune mechanisms","volume":"87","author":"Cox","year":"2002","journal-title":"Vet. Immunol. Immunpathol."},{"key":"10.1016\/j.dci.2010.06.009_bib11","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1051\/vetres\/2009023","article-title":"The food contaminant fumonisin B1 reduces the maturation of porcine CD11R1+ intestinal antigen presenting cells and antigen-specific immune responses, leading to a prolonged intestinal ETEC infection","volume":"40","author":"Devriendt","year":"2009","journal-title":"Vet. Res."},{"issue":"1","key":"10.1016\/j.dci.2010.06.009_bib12","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1079\/AHR2005105","article-title":"Escherichia coli in postweaning disease in pigs: an update on bacterial types, pathogenesis, and prevention strategies","volume":"6","author":"Fairbrother","year":"2005","journal-title":"Anim. Health Res. Rev."},{"key":"10.1016\/j.dci.2010.06.009_bib13","first-page":"251","article-title":"Hemorrhagic gastroenteritis caused by Escherichia coli in piglets: clinical, pathological and microbiological findings","volume":"33","author":"Faubert","year":"1992","journal-title":"Can. Vet. J."},{"key":"10.1016\/j.dci.2010.06.009_bib14","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0378-1135(01)00504-1","article-title":"Prevalence of serogroups and virulence genes in Escherichia coli associated with postweaning diarrhoea and edema disease in pigs and a comparison of diagnostic approaches","volume":"85","author":"Frydendahl","year":"2002","journal-title":"Vet. Microbiol."},{"issue":"3","key":"10.1016\/j.dci.2010.06.009_bib15","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1002\/jlb.59.3.407","article-title":"IL-8 induces T cell chemotaxis, suppresses IL-4, and up-regulates IL-8 production by CD4 (+) T cells","volume":"59","author":"Gesser","year":"1996","journal-title":"J. Leukoc. Biol."},{"key":"10.1016\/j.dci.2010.06.009_bib16","series-title":"Escherichia coli in Domestic Animals and Humans","first-page":"337","article-title":"Escherichia coli enterotoxins","author":"Gyles","year":"1994"},{"key":"10.1016\/j.dci.2010.06.009_bib17","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1038\/35074106","article-title":"The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5","volume":"410","author":"Hayashi","year":"2001","journal-title":"Nature"},{"issue":"2","key":"10.1016\/j.dci.2010.06.009_bib18","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/S0171-2985(00)80061-3","article-title":"Interleukin-6 signal transduction and lymphocyte function","volume":"202","author":"Horn","year":"2000","journal-title":"Immunobiology"},{"issue":"7","key":"10.1016\/j.dci.2010.06.009_bib19","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1007\/s10529-009-9966-8","article-title":"Secretory expression of K88 (F4) fimbrial adhesin FaeG by recombinant Lactococcus lactis for oral vaccination and its protective immune response in mice","volume":"31","author":"Hu","year":"2009","journal-title":"Biotechnol. Lett."},{"issue":"2","key":"10.1016\/j.dci.2010.06.009_bib20","doi-asserted-by":"crossref","first-page":"e4487","DOI":"10.1371\/journal.pone.0004487","article-title":"Sulfatide recognition by colonization factor antigen CS6 from enterotoxigenic Escherichia coli","volume":"4","author":"Jansson","year":"2009","journal-title":"PlosOne"},{"issue":"13","key":"10.1016\/j.dci.2010.06.009_bib21","doi-asserted-by":"crossref","first-page":"2387","DOI":"10.1016\/j.vaccine.2005.11.056","article-title":"F4 (K88) fimbrial adhesin FaeG expressed in alfalfa reduces F4+ enterotoxigenic Escherichia coli excretion in weaned piglets","volume":"24","author":"Joensuu","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.dci.2010.06.009_bib22","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1128\/JB.00822-08","article-title":"Heat-labile enterotoxin promotes Escherichia coli adherence to intestinal epithelial cells","volume":"191","author":"Johnson","year":"2009","journal-title":"J. Bacteriol."},{"issue":"12","key":"10.1016\/j.dci.2010.06.009_bib23","first-page":"S51","article-title":"Epithelial cells as sensors for microbial infection","volume":"100","author":"Kagnoff","year":"1997","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.dci.2010.06.009_bib24","doi-asserted-by":"crossref","first-page":"d340","DOI":"10.2741\/A136","article-title":"Molecular and cellular biology of interleukine-6 and its receptor","volume":"1","author":"Keller","year":"1996","journal-title":"Front. Biosci."},{"issue":"1\u20132","key":"10.1016\/j.dci.2010.06.009_bib25","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.vetmic.2007.12.018","article-title":"Porcine intestinal epithelial cell lines as a new in vitro model for studying adherence and pathogenesis of enterotoxigenic Escherichia coli","volume":"130","author":"Koh","year":"2008","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.dci.2010.06.009_bib26","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.vetimm.2009.04.006","article-title":"Gene expression study of two widely used pig intestinal epithelial cell lines: IPEC-J2 and IPI-2I","volume":"131","author":"Mariani","year":"2009","journal-title":"Vet. Immunol. Immunopathol."},{"issue":"5","key":"10.1016\/j.dci.2010.06.009_bib27","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1016\/j.molimm.2008.09.036","article-title":"Broad early immune response of porcine epithelial jejunal IPI-2I cells to Entamoeba histolytica","volume":"46","author":"Meurens","year":"2009","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.dci.2010.06.009_bib28","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.bbagen.2007.11.006","article-title":"Toll-like receptor 4 and cytokine expression involved in functional immune response in an originally established porcine intestinal epitheliocyte cell line","volume":"1780","author":"Moue","year":"2008","journal-title":"Biochim. Biophys. Acta"},{"issue":"2","key":"10.1016\/j.dci.2010.06.009_bib29","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1038\/nri1777","article-title":"Mucosal vaccines: the promise and the challenge","volume":"6","author":"Neutra","year":"2006","journal-title":"Nat. Rev. Immunol."},{"issue":"1\u20132","key":"10.1016\/j.dci.2010.06.009_bib30","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.vetmic.2008.04.024","article-title":"Cytokine mRNA expression in porcine cell lines stimulated by enterotoxigenic Escherichia coli","volume":"132","author":"Pavlova","year":"2008","journal-title":"Vet. Microb."},{"issue":"3","key":"10.1016\/j.dci.2010.06.009_bib31","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1128\/CMR.18.3.465-483.2005","article-title":"Enterotoxigenic Escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment, and prevention","volume":"18","author":"Qadri","year":"2005","journal-title":"Clin. Microbiol. Rev."},{"issue":"11","key":"10.1016\/j.dci.2010.06.009_bib32","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.tim.2004.09.002","article-title":"Bacterial flagellins: mediators of pathogenicity and host immune responses in mucosa","volume":"12","author":"Ramos","year":"2004","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.dci.2010.06.009_bib33","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1099\/jmm.0.05413-0","article-title":"Prevalence of childhood diarhea-associated Escherichia coli in Thailand","volume":"53","author":"Ratchtrachenchai","year":"2004","journal-title":"J. Med. Microbiol."},{"issue":"4","key":"10.1016\/j.dci.2010.06.009_bib34","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1038\/86373","article-title":"Dendritic cells express tight junctions proteins and penetrate gut epithelial monolayers to sample bacteria","volume":"2","author":"Rescigno","year":"2001","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.dci.2010.06.009_bib35","doi-asserted-by":"crossref","first-page":"3684","DOI":"10.4049\/jimmunol.171.7.3684","article-title":"CD11b+ Peyer's Patch dendritic cells secrete IL-6 and induce IgA secretion from na\u00efve B cells","volume":"171","author":"Sato","year":"2003","journal-title":"J. Immunol."},{"key":"10.1016\/j.dci.2010.06.009_bib36","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1038\/nri1499","article-title":"War and peace at mucosal surfaces","volume":"4","author":"Sansonetti","year":"2004","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.dci.2010.06.009_bib37","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1007\/s00418-005-0067-z","article-title":"Characterization of a porcine intestinal epithelial cell line for in vitro studies of microbial pathogenesis in swine","volume":"125","author":"Schierack","year":"2006","journal-title":"Histochem. Cell Biol."},{"issue":"24","key":"10.1016\/j.dci.2010.06.009_bib38","doi-asserted-by":"crossref","first-page":"13722","DOI":"10.1073\/pnas.241308598","article-title":"Flagellin stimulation of intestinal epithelial cells triggers CCL20-mediated migration of dendritic cells","volume":"98","author":"Sierro","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.dci.2010.06.009_bib39","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1172\/JCI11783","article-title":"Neutrophil-epithelial crosstalk at the intestinal lumenal surface mediated by reciprocal secretion of adenosine and IL-6","volume":"107","author":"Sitaraman","year":"2001","journal-title":"J. Clin. Invest."},{"issue":"3\u20134","key":"10.1016\/j.dci.2010.06.009_bib40","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/j.vetimm.2006.10.012","article-title":"Effects of Salmonella enterica serovar Typhimurium, or serovar Choleraesuis, Lactobacillus reuteri and Bacillus licheniformis on chemokine and cytokine expression in the swine jejunal epithelial cell line, IPEC-J2","volume":"115","author":"Skjolaas","year":"2007","journal-title":"Vet. Immunol. Immunopathol."},{"issue":"1","key":"10.1016\/j.dci.2010.06.009_bib41","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.vetmic.2003.10.020","article-title":"Influence of porcine intestinal pH and gastric digestion on antigenicity of F4 fimbriae for oral immunisation","volume":"98","author":"Snoeck","year":"2004","journal-title":"Vet. Microbiol."},{"issue":"18","key":"10.1016\/j.dci.2010.06.009_bib42","doi-asserted-by":"crossref","first-page":"3812","DOI":"10.1016\/j.vaccine.2005.07.025","article-title":"The jejunal Peyer's patches are the major inductive sites of the F4-specific immune response following intestinal immunisation of pigs with F4 (K88) fimbriae","volume":"24","author":"Snoeck","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.dci.2010.06.009_bib43","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.vetimm.2006.10.014","article-title":"Transcytosis of F4 fimbriae by villous and dome epithelia in F4-receptor positive pigs supports the importance of receptor-dependent endocytosis in oral immunization strategies","volume":"124","author":"Snoeck","year":"2008","journal-title":"Vet. Immunol. Immunpathol."},{"issue":"2","key":"10.1016\/j.dci.2010.06.009_bib44","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1128\/IAI.67.2.520-526.1999","article-title":"Receptor-dependent immune responses in pigs after oral immunization with F4 fimbriae","volume":"67","author":"Van den Broeck","year":"1999","journal-title":"Infect. Immun."},{"issue":"3\u20134","key":"10.1016\/j.dci.2010.06.009_bib45","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0378-1135(99)00076-0","article-title":"Receptor-specific binding of purified F4 fimbriae to isolated villi","volume":"68","author":"Van den Broeck","year":"1999","journal-title":"Vet. Microbiol."},{"issue":"3","key":"10.1016\/j.dci.2010.06.009_bib46","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S0378-1135(99)00108-X","article-title":"Seroprevalence of F4+ enterotoxigenic Escherichia coli in regions with different pig farm densities","volume":"69","author":"Van den Broeck","year":"1999","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.dci.2010.06.009_bib47","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1016\/j.jmb.2007.02.051","article-title":"Chloroplast assemble the major subunit FaeG of Escherichia coli F4 (K88) fimbriae to strand-swapped dimers","volume":"368","author":"Van Molle","year":"2007","journal-title":"J. Mol. Biol."},{"issue":"32\u201332","key":"10.1016\/j.dci.2010.06.009_bib48","doi-asserted-by":"crossref","first-page":"4291","DOI":"10.1016\/j.vaccine.2004.04.016","article-title":"Oral immunization of piglets with recombinant F4 fimbrial adhesin FaeG monomers induces a mucosal and systemic F4-specific immune response","volume":"22","author":"Verdonck","year":"2004","journal-title":"Vaccine"},{"key":"10.1016\/j.dci.2010.06.009_bib49","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.vetmic.2004.06.002","article-title":"Conserved regions in the sequence of the F4 (K88) fimbrial adhesin FaeG suggest a donor strand mechanism in F4 assembly","volume":"102","author":"Verdonck","year":"2004","journal-title":"Vet. Microbiol."},{"issue":"45","key":"10.1016\/j.dci.2010.06.009_bib50","doi-asserted-by":"crossref","first-page":"5728","DOI":"10.1016\/j.vaccine.2008.08.017","article-title":"The polymeric stability of the Escherichia coli F4 (K88) fimbriae enhances its mucosal immunogenicity following oral immunization","volume":"26","author":"Verdonck","year":"2008","journal-title":"Vaccine"},{"issue":"1","key":"10.1016\/j.dci.2010.06.009_bib51","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.ijfoodmicro.2008.12.037","article-title":"Differential crosstalk between epithelial cells, dendritic cells and bacteria in a co-culture model","volume":"131","author":"Zoumpopoulou","year":"2009","journal-title":"Int. J. Food Microbiol."}],"container-title":["Developmental & Comparative Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0145305X10001357?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0145305X10001357?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,6,6]],"date-time":"2020-06-06T11:23:28Z","timestamp":1591442608000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0145305X10001357"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11]]},"references-count":51,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2010,11]]}},"alternative-id":["S0145305X10001357"],"URL":"https:\/\/doi.org\/10.1016\/j.dci.2010.06.009","relation":{},"ISSN":["0145-305X"],"issn-type":[{"value":"0145-305X","type":"print"}],"subject":[],"published":{"date-parts":[[2010,11]]}}}