{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T02:03:12Z","timestamp":1784080992585,"version":"3.55.0"},"reference-count":38,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100012336","name":"Major State Basic Research Development Program of China","doi-asserted-by":"crossref","award":["2012CB114403"],"award-info":[{"award-number":["2012CB114403"]}],"id":[{"id":"10.13039\/501100012336","id-type":"DOI","asserted-by":"crossref"}]},{"name":"General Program of National Natural Science Foundation of China","award":["31272683"],"award-info":[{"award-number":["31272683"]}]},{"name":"Agro-scientific Research in the Public Interest","award":["201103034"],"award-info":[{"award-number":["201103034"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Developmental & Comparative Immunology"],"published-print":{"date-parts":[[2015,11]]},"DOI":"10.1016\/j.dci.2015.06.015","type":"journal-article","created":{"date-parts":[[2015,6,27]],"date-time":"2015-06-27T19:25:16Z","timestamp":1435433116000},"page":"47-54","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":44,"title":["Recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (FcALF5) from Chinese shrimp Fenneropenaeus chinensis"],"prefix":"10.1016","volume":"53","author":[{"given":"Hui","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shihao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuhua","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinjia","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhai","family":"Xiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.dci.2015.06.015_bib1","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1016\/S0044-8486(03)00521-0","article-title":"Anti-infectious immune effectors in marine invertebrates: potential tools for disease control in larviculture","volume":"227","author":"Bach\u00e8re","year":"2003","journal-title":"Aquaculture"},{"key":"10.1016\/j.dci.2015.06.015_bib2","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1016\/S0145-305X(99)00015-4","article-title":"Antimicrobial peptides in insects; structure and function","volume":"23","author":"Bulet","year":"1999","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.dci.2015.06.015_bib3","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1590\/S0074-02762007005000028","article-title":"In\u00a0vitro antiviral activity of antimicrobial peptides against herpes simplex virus 1, adenovirus, and rotavirus","volume":"102","author":"Carriel-Gomes","year":"2007","journal-title":"Mem. Inst. Oswaldo. Cruz"},{"issue":"5","key":"10.1016\/j.dci.2015.06.015_bib4","doi-asserted-by":"publisher","first-page":"e10718","DOI":"10.1371\/journal.pone.0010718","article-title":"A\u00a03D model of the membrane protein complex formed by the white spot syndrome virus structural proteins","volume":"5","author":"Chang","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.dci.2015.06.015_bib5","doi-asserted-by":"publisher","first-page":"434","DOI":"10.1016\/j.fsi.2009.11.020","article-title":"Antimicrobial peptides (AMP) with antiviral activity against fish nodavirus","volume":"28","author":"Chia","year":"2010","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.dci.2015.06.015_bib6","doi-asserted-by":"publisher","first-page":"1916","DOI":"10.1016\/j.molimm.2007.10.039","article-title":"Anti-lipopolysaccharide factor in Litopenaeus vannamei (LvALF): a broad spectrum antimicrobial peptide essential for shrimp immunity against bacterial and fungal infection","volume":"45","author":"de la Vega","year":"2008","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.dci.2015.06.015_bib7","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1016\/j.dci.2014.03.003","article-title":"Modification of a synthetic LPS-binding domain of anti-lipopolysaccharide factor from shrimp reveals strong structure-activity relationship in their antimicrobial characteristics","volume":"45","author":"Guo","year":"2014","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.dci.2015.06.015_bib8","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1016\/S0966-842X(00)01823-0","article-title":"The role of cationic antimicrobial peptides in innate host defences","volume":"8","author":"Hancock","year":"2000","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.dci.2015.06.015_bib9","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/S0167-7799(97)01155-4","article-title":"Strategies for optimizing heterologous protein expression in Escherichia coli","volume":"16","author":"Hannig","year":"1998","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.dci.2015.06.015_bib10","doi-asserted-by":"crossref","first-page":"3351","DOI":"10.1002\/j.1460-2075.1993.tb06008.x","article-title":"Crystal-Structure of an endotoxin-neutralizing protein from the horseshoe-crab, limulus anti-Lps factor, at 1.5 angstrom resolution","volume":"12","author":"Hoess","year":"1993","journal-title":"Embo. J."},{"key":"10.1016\/j.dci.2015.06.015_bib11","first-page":"741","article-title":"Anti-lipopolysaccharide factor evokes indirect killing of virulent bacteria in kuruma prawn","volume":"25","author":"Kadowaki","year":"2011","journal-title":"Vivo"},{"key":"10.1016\/j.dci.2015.06.015_bib12","doi-asserted-by":"publisher","first-page":"1654","DOI":"10.1093\/infdis\/jis273","article-title":"A\u00a0new class of synthetic peptide inhibitors blocks attachment and entry of human pathogenic viruses","volume":"205","author":"Krepstakies","year":"2012","journal-title":"J.\u00a0Infect. Dis."},{"key":"10.1016\/j.dci.2015.06.015_bib13","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1111\/j.1472-765X.1996.tb01121.x","article-title":"Isolation and characterization of Vibrio alginolyticus isolated from diseased kuruma prawn, Penaeus japonicus","volume":"22","author":"Lee","year":"1996","journal-title":"Lett. Appl. Microbiol."},{"key":"10.1016\/j.dci.2015.06.015_bib14","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/j.dci.2014.05.013","article-title":"Characterization and function analysis of an anti-lipopolysaccharide factor (ALF) from the Chinese shrimpFenneropenaeus chinensis","volume":"46","author":"Li","year":"2014","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.dci.2015.06.015_bib15","doi-asserted-by":"publisher","first-page":"2602","DOI":"10.3390\/md13052602","article-title":"Functional diversity of anti-lipopolysaccharide factor isoforms in shrimp and their characters related to antiviral activity","volume":"13","author":"Li","year":"2015","journal-title":"Mar. Drugs"},{"key":"10.1016\/j.dci.2015.06.015_bib16","doi-asserted-by":"publisher","first-page":"e58627","DOI":"10.1371\/journal.pone.0058627","article-title":"Transcriptome analysis on Chinese shrimp Fenneropenaeus chinensis during WSSV acute infection","volume":"8","author":"Li","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.dci.2015.06.015_bib17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jip.2013.12.001","article-title":"Function of an anti-lipopolysaccharide factor (ALF) isoform isolated from the hemocytes of the giant freshwater prawn Macrobrachium rosenbergii in protecting against bacterial infection","volume":"116","author":"Liu","year":"2014","journal-title":"J.\u00a0Invertebr. Pathol."},{"key":"10.1016\/j.dci.2015.06.015_bib18","doi-asserted-by":"publisher","first-page":"600","DOI":"10.1007\/s10126-005-5006-4","article-title":"Molecular cloning and expression profile of putative antilipopolysaccharide factor in Chinese shrimp (Fenneropenaeus chinensis)","volume":"7","author":"Liu","year":"2005","journal-title":"Mar. Biotechnol."},{"key":"10.1016\/j.dci.2015.06.015_bib19","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.fsi.2012.04.004","article-title":"Molecular cloning, expression pattern and antimicrobial activity of a new isoform of anti-lipopolysaccharide factor from the swimming crab Portunus trituberculatus","volume":"33","author":"Liu","year":"2012","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.dci.2015.06.015_bib20","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1016\/j.fsi.2010.12.005","article-title":"Three isoforms of anti-lipopolysaccharide factor identified from eyestalk cDNA library of swimming crab Portunus trituberculatus","volume":"30","author":"Liu","year":"2011","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.dci.2015.06.015_bib21","doi-asserted-by":"publisher","first-page":"53","DOI":"10.3354\/dao030053","article-title":"Detection and tissue tropism of white spot syndrome baculovirus (WSBV) in captured brooders of Penaeus monodon with a special emphasis on reproductive organs","volume":"30","author":"Lo","year":"1997","journal-title":"Dis. Aquat. Organ"},{"key":"10.1016\/j.dci.2015.06.015_bib22","doi-asserted-by":"publisher","first-page":"1611","DOI":"10.1093\/oxfordjournals.jbchem.a135218","article-title":"Isolation and biological-activities of limulus anticoagulant (Anti-Lps factor) which interacts with lipopolysaccharide (Lps)","volume":"97","author":"Morita","year":"1985","journal-title":"J.\u00a0Biochem."},{"key":"10.1016\/j.dci.2015.06.015_bib23","doi-asserted-by":"publisher","first-page":"321","DOI":"10.3389\/fmicb.2013.00321","article-title":"Current scenario of peptide-based drugs: the key roles of cationic antitumor and antiviral peptides","volume":"4","author":"Mulder","year":"2013","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.dci.2015.06.015_bib24","doi-asserted-by":"publisher","first-page":"2061","DOI":"10.1016\/j.molimm.2005.12.009","article-title":"Cloning and characterization of a LPS-regulatory gene having an LPS binding domain in kuruma prawn Marsupenaeus japonicus","volume":"43","author":"Nagoshi","year":"2006","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.dci.2015.06.015_bib25","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/j.aquaculture.2009.01.026","article-title":"Recombinant anti-lipopolysaccharide factor isoform 3 and the prevention of vibriosis in the black tiger shrimp, Penaeus monodon","volume":"289","author":"Ponprateep","year":"2009","journal-title":"Aquaculture"},{"key":"10.1016\/j.dci.2015.06.015_bib26","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/j.fsi.2011.10.010","article-title":"Gene silencing reveals a crucial role for anti-lipopolysaccharide factors from Penaeus monodon in the protection against microbial infections","volume":"32","author":"Ponprateep","year":"2012","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.dci.2015.06.015_bib27","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1016\/j.aquaculture.2012.11.014","article-title":"Analysis on the dynamic changes of the amount of WSSV in Chinese shrimp Fenneropenaeus chinensis during infection","volume":"376","author":"Sun","year":"2013","journal-title":"Aquaculture"},{"key":"10.1016\/j.dci.2015.06.015_bib28","doi-asserted-by":"publisher","first-page":"123","DOI":"10.3354\/dao061123","article-title":"Antimicrobial peptides discovered in the black tiger shrimp Penaeus monodon using the EST approach","volume":"61","author":"Supungul","year":"2004","journal-title":"Dis. Aquat. Organ"},{"key":"10.1016\/j.dci.2015.06.015_bib29","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1016\/0006-291X(82)91493-0","article-title":"Limulus anti-Lps factor - an anticoagulant which inhibits the endotoxin-mediated activation of limulus coagulation system","volume":"105","author":"Tanaka","year":"1982","journal-title":"Biochem. Bioph. Res. Co."},{"key":"10.1016\/j.dci.2015.06.015_bib30","doi-asserted-by":"publisher","first-page":"1491","DOI":"10.1099\/vir.0.009621-0","article-title":"Role of anti-lipopolysaccharide factor from the black tiger shrimp, Penaeus monodon, in protection from white spot syndrome virus infection","volume":"90","author":"Tharntada","year":"2009","journal-title":"J.\u00a0Gen. Virol."},{"key":"10.1016\/j.dci.2015.06.015_bib31","doi-asserted-by":"publisher","first-page":"12598","DOI":"10.1128\/JVI.01732-08","article-title":"VP26 of White spot syndrome virus functions as a linker protein between the envelope and nucleocapsid of virions by binding with VP51","volume":"82","author":"Wan","year":"2008","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.dci.2015.06.015_bib32","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/j.dci.2012.10.012","article-title":"Molecular characterization, tissue distribution and functional analysis of macrophage migration inhibitory factor protein (MIF) in Chinese giant salamanders Andrias davidianus","volume":"39","author":"Wang","year":"2013","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.dci.2015.06.015_bib33","doi-asserted-by":"publisher","first-page":"1903","DOI":"10.1099\/vir.0.81570-0","article-title":"White spot syndrome virus VP24 interacts with VP28 and is involved in virus infection","volume":"87","author":"Xie","year":"2006","journal-title":"J.\u00a0Gen. Virol."},{"key":"10.1016\/j.dci.2015.06.015_bib34","doi-asserted-by":"publisher","first-page":"11811","DOI":"10.1128\/JVI.75.23.11811-11820.2001","article-title":"Complete genome sequence of the shrimp white spot bacilliform virus","volume":"75","author":"Yang","year":"2001","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.dci.2015.06.015_bib35","first-page":"726","article-title":"VP28 of white spot syndrome virus is involved in the attachment and penetration into shrimp cells","volume":"37","author":"Yi","year":"2004","journal-title":"J.\u00a0Biochem. Mol. Biol."},{"key":"10.1016\/j.dci.2015.06.015_bib36","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1002\/bip.20543","article-title":"Advances in antimicrobial peptide immunobiology","volume":"84","author":"Yount","year":"2006","journal-title":"Biopolymers"},{"key":"10.1016\/j.dci.2015.06.015_bib37","first-page":"162","article-title":"The antimicrobial peptides of the immune response of shrimp","volume":"5","author":"Zhao","year":"2008","journal-title":"Inv. Survl. J."},{"key":"10.1016\/j.dci.2015.06.015_bib38","doi-asserted-by":"publisher","first-page":"4709","DOI":"10.1128\/JVI.02360-08","article-title":"Four major envelope proteins of white spot syndrome virus bind to form a complex","volume":"83","author":"Zhou","year":"2009","journal-title":"J.\u00a0Virol."}],"container-title":["Developmental & Comparative Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0145305X15300033?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0145305X15300033?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T11:37:03Z","timestamp":1589542623000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0145305X15300033"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["S0145305X15300033"],"URL":"https:\/\/doi.org\/10.1016\/j.dci.2015.06.015","relation":{},"ISSN":["0145-305X"],"issn-type":[{"value":"0145-305X","type":"print"}],"subject":[],"published":{"date-parts":[[2015,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (FcALF5) from Chinese shrimp Fenneropenaeus chinensis","name":"articletitle","label":"Article Title"},{"value":"Developmental & Comparative Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.dci.2015.06.015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}