{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T14:06:24Z","timestamp":1785247584516,"version":"3.55.0"},"reference-count":62,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"name":"Modern Agriculture Industry Technology System Construction Projects of China Staple Freshwater Fishes Industry Technology System","award":["CARS\u201346\u201305"],"award-info":[{"award-number":["CARS\u201346\u201305"]}]},{"name":"Fundament Research Funds for the Central Universities","award":["2662015PY019"],"award-info":[{"award-number":["2662015PY019"]}]},{"name":"Scientific Research and Development program of Hubei Province","award":["2013BHE006"],"award-info":[{"award-number":["2013BHE006"]}]},{"name":"The International Scientific and Technology Cooperation Program of Wuhan City","award":["2015030809020365"],"award-info":[{"award-number":["2015030809020365"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Developmental & Comparative Immunology"],"published-print":{"date-parts":[[2016,6]]},"DOI":"10.1016\/j.dci.2016.01.009","type":"journal-article","created":{"date-parts":[[2016,1,21]],"date-time":"2016-01-21T11:30:12Z","timestamp":1453375812000},"page":"63-76","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":27,"special_numbering":"C","title":["Molecular cloning and expression of toll-like receptor 4 (tlr4) in the blunt snout bream (Megalobrama amblycephala)"],"prefix":"10.1016","volume":"59","author":[{"given":"Ruifang","family":"Lai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2461-3712","authenticated-orcid":false,"given":"Ivan","family":"Jakovli\u0107","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanbin","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinhong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weimin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.dci.2016.01.009_bib1","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S1074-7613(00)80596-8","article-title":"Targeted disruption of the MyD88 gene results in loss of IL-1-and IL-18-mediated function","volume":"9","author":"Adachi","year":"1998","journal-title":"Immunity"},{"key":"10.1016\/j.dci.2016.01.009_bib2","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1038\/nri1391","article-title":"Toll-like receptor signaling","volume":"4","author":"Akira","year":"2004","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib3","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.cell.2006.02.015","article-title":"Pathogen recognition and innate immunity","volume":"124","author":"Akira","year":"2006","journal-title":"Cell"},{"key":"10.1016\/j.dci.2016.01.009_bib4","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1016\/j.fsi.2007.06.002","article-title":"Divergent toll-like receptors in catfish (Ictalurus punctatus): TLR5S, TLR20, TLR21","volume":"23","author":"Baoprasertkul","year":"2007","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib5","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.fsi.2011.10.031","article-title":"Inductive expression of toll-like receptor 5 (TLR5) and associated downstream signaling molecules following ligand exposure and bacterial infection in the Indian major carp, mrigal (Cirrhinus mrigala)","volume":"32","author":"Basu","year":"2012","journal-title":"Fish. Shellfish Immun."},{"key":"10.1016\/j.dci.2016.01.009_bib6","doi-asserted-by":"crossref","first-page":"6015","DOI":"10.1007\/s11033-011-1415-9","article-title":"Induction of toll-like receptor (TLR) 2, and MyD88-dependent TLR-signaling in response to ligand stimulation and bacterial infections in the Indian major carp, mrigal (Cirrhinus mrigala)","volume":"39","author":"Basu","year":"2012","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.dci.2016.01.009_bib7","series-title":"China Fisheries Yearbook","author":"Bureau, C.F","year":"2013"},{"key":"10.1016\/j.dci.2016.01.009_bib8","doi-asserted-by":"crossref","first-page":"e42637","DOI":"10.1371\/journal.pone.0042637","article-title":"Transcriptome analysis and SSR\/SNP markers information of the blunt snout bream (Megalobrama amblycephala)","volume":"7","author":"Gao","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.dci.2016.01.009_bib9","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1007\/s00239-004-2613-z","article-title":"Phylogenetic timing of the fish-specific genome duplication correlates with the diversification of teleost fish","volume":"59","author":"Hoegg","year":"2004","journal-title":"J.\u00a0Mol. Evol."},{"key":"10.1016\/j.dci.2016.01.009_bib10","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.dci.2012.06.002","article-title":"Isolation and analysis of a novel grass carp toll-like receptor 4 (tlr4) gene cluster involved in the response to grass carp reovirus","volume":"38","author":"Huang","year":"2012","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib11","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.vetimm.2004.11.003","article-title":"Expression patterns of chicken Toll-like receptor mRNA in tissues, immune cell subsets and cell lines","volume":"104","author":"Iqbal","year":"2005","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.dci.2016.01.009_bib12","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1146\/annurev.immunol.20.083001.084359","article-title":"Innate immune recognition","volume":"20","author":"Janeway","year":"2002","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib13","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/j.molimm.2003.10.001","article-title":"Toll-like receptor gene family and TIR-domain adapters in Danio rerio","volume":"40","author":"Jault","year":"2004","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib14","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S1074-7613(00)80086-2","article-title":"Unresponsiveness of MyD88-deficient mice to endotoxin","volume":"11","author":"Kawai","year":"1999","journal-title":"Immunity"},{"key":"10.1016\/j.dci.2016.01.009_bib15","doi-asserted-by":"crossref","first-page":"5887","DOI":"10.4049\/jimmunol.167.10.5887","article-title":"Lipopolysaccharide stimulates the MyD88-independent pathway and results in activation of IFN-regulatory factor 3 and the expression of a subset of lipopolysaccharide-inducible genes","volume":"167","author":"Kawai","year":"2001","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib16","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2014.00461","article-title":"Toll-like receptor signaling pathways","volume":"5","author":"Kawasaki","year":"2014","journal-title":"Front. Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib17","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1002\/aja.1002030302","article-title":"Stages of embryonic development of the zebrafish","volume":"203","author":"Kimmel","year":"1995","journal-title":"Dev. Dyn."},{"key":"10.1016\/j.dci.2016.01.009_bib18","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1038\/374183a0","article-title":"A\u00a0structural basis of the interactions between leucine-rich repeats and protein ligands","author":"Kobe","year":"1995","journal-title":"Nature"},{"key":"10.1016\/j.dci.2016.01.009_bib19","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.fsi.2010.04.013","article-title":"SNP discovery and development of genetic markers for mapping innate immune response genes in common carp (Cyprinus carpio)","volume":"29","author":"Kongchum","year":"2010","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib20","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1111\/jam.12663","article-title":"Comparative study on gastrointestinal microbiota of eight fish species with different feeding habits","volume":"117","author":"Li","year":"2014","journal-title":"J.\u00a0Appl. Microbiol."},{"key":"10.1016\/j.dci.2016.01.009_bib21","series-title":"China Fisheries Yearbook","author":"Liu","year":"2009"},{"key":"10.1016\/j.dci.2016.01.009_bib22","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.diabres.2014.04.020","article-title":"Expression and cellular distribution of TLR4, MyD88, and NF-\u03baB in diabetic renal tubulointerstitial fibrosis, in\u00a0vitro and in\u00a0vivo","volume":"105","author":"Liu","year":"2014","journal-title":"Diabetes Res. Clin. P. R."},{"key":"10.1016\/j.dci.2016.01.009_bib23","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394Ct method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.dci.2016.01.009_bib24","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.cyto.2008.01.006","article-title":"LPS\/TLR4 signal transduction pathway","volume":"42","author":"Lu","year":"2008","journal-title":"Cytokine"},{"key":"10.1016\/j.dci.2016.01.009_bib25","first-page":"20","article-title":"Toll-like receptor (TLR) 4 in mrigal (Cirrhinus mrigala): response to lipopolysaccharide treatment and Aeromonas hydrophila infection","volume":"2","author":"Madhubanti","year":"2013","journal-title":"Int. J. Biol. Sci."},{"key":"10.1016\/j.dci.2016.01.009_bib26","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.fsi.2004.09.006","article-title":"Innate immunity of fish (overview)","volume":"20","author":"Magnadottir","year":"2006","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib27","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1038\/35100529","article-title":"Toll-like receptors and innate immunity","volume":"1","author":"Medzhitov","year":"2001","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib28","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1038\/41131","article-title":"A\u00a0human homologue of the Drosophila toll protein signals activation of adaptive immunity","volume":"388","author":"Medzhitov","year":"1997","journal-title":"Nature"},{"key":"10.1016\/j.dci.2016.01.009_bib29","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/S1097-2765(00)80136-7","article-title":"MyD88 is an adaptor protein in the hToll\/IL-1 receptor family signaling pathways","volume":"2","author":"Medzhitov","year":"1998","journal-title":"Mol. Cell"},{"key":"10.1016\/j.dci.2016.01.009_bib30","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1016\/j.molimm.2003.10.003","article-title":"Expression analysis of the Toll-like receptor and TIR domain adaptor families of zebrafish","volume":"40","author":"Meijer","year":"2004","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/protein\/10.1.1","article-title":"Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites","volume":"10","author":"Nielsen","year":"1997","journal-title":"Protein Eng."},{"key":"10.1016\/j.dci.2016.01.009_bib32","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3354\/dao046023","article-title":"Is Aeromonas hydrophila the dominant motile Aeromonas species that causes disease outbreaks in aquaculture production in the Zhejiang Province of China?","volume":"46","author":"Nielsen","year":"2001","journal-title":"Dis. Aquat. Organ"},{"key":"10.1016\/j.dci.2016.01.009_bib33","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1038\/nri2079","article-title":"The family of five: TIR-domain-containing adaptors in Toll-like receptor signaling","volume":"7","author":"O'Neill","year":"2007","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib34","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.tins.2011.02.005","article-title":"Toll-like receptor signaling in neural plasticity and disease","volume":"34","author":"Okun","year":"2011","journal-title":"Trends Neurosci."},{"key":"10.1016\/j.dci.2016.01.009_bib35","doi-asserted-by":"crossref","first-page":"e47522","DOI":"10.1371\/journal.pone.0047522","article-title":"Evidence for a developmental role for TLR4 in learning and memory","volume":"7","author":"Okun","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.dci.2016.01.009_bib36","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1007\/s00251-002-0519-8","article-title":"Prediction of the prototype of the human Toll-like receptor gene family from the pufferfish, Fugu rubripes, genome","volume":"54","author":"Oshiumi","year":"2003","journal-title":"Immunogenetics"},{"key":"10.1016\/j.dci.2016.01.009_bib37","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.1111\/jfb.12617","article-title":"Characterizations of four toll-like receptor 4s in grass carp Ctenopharyngodon idellus and their response to grass carp reovirus infection and lipopolysaccharide stimulation","volume":"86","author":"Pei","year":"2015","journal-title":"J.\u00a0Fish. Biol."},{"key":"10.1016\/j.dci.2016.01.009_bib38","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1126\/science.282.5396.2085","article-title":"Defective LPS signaling in C3H\/HeJ and C57BL\/10ScCr mice: mutations in Tlr4 gene","volume":"282","author":"Poltorak","year":"1998","journal-title":"Science"},{"key":"10.1016\/j.dci.2016.01.009_bib39","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1007\/s00251-013-0694-9","article-title":"Comprehensive survey and genomic characterization of Toll-like receptors (TLRs) in channel catfish, Ictalurus punctatus: identification of novel fish TLRs","volume":"65","author":"Quiniou","year":"2013","journal-title":"Immunogenetics"},{"key":"10.1016\/j.dci.2016.01.009_bib40","doi-asserted-by":"crossref","first-page":"4697","DOI":"10.4049\/jimmunol.169.9.4697","article-title":"Cutting edge: impaired Toll-like receptor expression and function in aging","volume":"169","author":"Renshaw","year":"2002","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib41","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1095\/biolreprod.108.069930","article-title":"Activation of Toll-like receptor 4 (TLR4) by in\u00a0vivo and in\u00a0vitro exposure of rat epididymis to lipopolysaccharide from Escherichia coli","volume":"79","author":"Rodrigues","year":"2008","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.dci.2016.01.009_bib42","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1038\/ncb1629","article-title":"Toll-like receptors modulate adult hippocampal neurogenesis","volume":"9","author":"Rolls","year":"2007","journal-title":"Nat. Cell Boil"},{"key":"10.1016\/j.dci.2016.01.009_bib43","first-page":"406","article-title":"The neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.dci.2016.01.009_bib44","doi-asserted-by":"crossref","first-page":"8104","DOI":"10.1186\/1749-8104-8-3","article-title":"Neurogenesis in zebrafish-from embryo to adult","volume":"8","author":"Schmidt","year":"2013","journal-title":"Neural Dev."},{"key":"10.1016\/j.dci.2016.01.009_bib45","doi-asserted-by":"crossref","first-page":"5857","DOI":"10.1073\/pnas.95.11.5857","article-title":"SMART, a simple modular architecture research tool: identification of signaling domains","volume":"95","author":"Schultz","year":"1998","journal-title":"P.\u00a0Natl. Acad. Sci."},{"key":"10.1016\/j.dci.2016.01.009_bib46","first-page":"3","article-title":"The innate and adaptive immune system of fish","volume":"231","author":"Secombes","year":"2012","journal-title":"Infect. Dis. Aquac. Prev. Control"},{"key":"10.1016\/j.dci.2016.01.009_bib47","doi-asserted-by":"crossref","first-page":"1836","DOI":"10.4049\/jimmunol.0801755","article-title":"Evolution of lipopolysaccharide (LPS) recognition, signaling: fish TLR4 does not recognize LPS, negatively regulates NFkappaB activation","volume":"182","author":"Sepulcre","year":"2009","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib48","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/0092-8674(86)90341-7","article-title":"A\u00a0conserved AU sequence from the 3\u02b9 untranslated region of GM-CSF mRNA mediates selective mRNA degradation","volume":"46","author":"Shaw","year":"1986","journal-title":"Cell"},{"key":"10.1016\/j.dci.2016.01.009_bib49","doi-asserted-by":"crossref","first-page":"4670","DOI":"10.1074\/jbc.275.7.4670","article-title":"Identification of two major sites in the type I interleukin-1 receptor cytoplasmic region responsible for coupling to pro-inflammatory signaling pathways","volume":"275","author":"Slack","year":"2000","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.dci.2016.01.009_bib50","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.fsi.2009.02.016","article-title":"Toll-like receptor 4 signaling pathway can be triggered by grass carp reovirus, Aeromonas hydrophila infection in rare minnow Gobiocypris rarus","volume":"27","author":"Su","year":"2009","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib51","doi-asserted-by":"crossref","first-page":"5896","DOI":"10.4049\/jimmunol.0803285","article-title":"The gene history of zebrafish tlr4a and tlr4b is predictive of their divergent functions","volume":"183","author":"Sullivan","year":"2009","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib52","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1046\/j.1462-5822.2003.00264.x","article-title":"Toll receptors and pathogen resistance","volume":"5","author":"Takeda","year":"2003","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.dci.2016.01.009_bib53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/intimm\/dxh186","article-title":"Toll-like receptors in innate immunity","volume":"17","author":"Takeda","year":"2005","journal-title":"Int. Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib54","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1093\/molbev\/mst197","article-title":"MEGA6: molecular evolutionary genetics analysis version 6.0","volume":"30","author":"Tamura","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.dci.2016.01.009_bib55","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.fsi.2015.06.023","article-title":"Transcriptome-wide identification, molecular evolution and expression analysis of Toll-like receptor family in a Tibet fish, Gymnocypris przewalskii","volume":"46","author":"Tong","year":"2015","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib56","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.fsi.2015.01.034","article-title":"Transcriptome analysis and microsatellite discovery in the blunt snout bream (Megalobrama amblycephala) after challenge with Aeromonas hydrophila","volume":"45","author":"Tran","year":"2015","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib57","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1124\/mi.3.8.466","article-title":"TLRs: differential adapter utilization by Toll-like receptors mediates TLR-specific patterns off gene expression","volume":"3","author":"Vogel","year":"2003","journal-title":"Mol. Interv."},{"key":"10.1016\/j.dci.2016.01.009_bib58","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.vetimm.2011.02.016","article-title":"Cloning and expression of Toll-like receptors 1 and 2 from a teleost fish, the orange-spotted grouper Epinephelus coioides","volume":"141","author":"Wei","year":"2011","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.dci.2016.01.009_bib59","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.fsi.2008.10.014","article-title":"Molecular cloning and expression of MyD88 in large yellow croaker, Pseudosciaena crocea","volume":"26","author":"Yao","year":"2009","journal-title":"Fish. Shellfish Immun."},{"key":"10.1016\/j.dci.2016.01.009_bib60","first-page":"103","article-title":"Observations on the embryonic development of Megalobrama amblycephala in the fishing areas of Poyang lake","volume":"5","author":"Yu","year":"1998","journal-title":"J.\u00a0Fish. Sci. China"},{"key":"10.1016\/j.dci.2016.01.009_bib61","doi-asserted-by":"crossref","first-page":"554","DOI":"10.4049\/jimmunol.168.2.554","article-title":"Tissue expression of human Toll-like receptors and differential regulation of Toll-like receptor mRNAs in leukocytes in response to microbes, their products, and cytokines","volume":"168","author":"Zarember","year":"2002","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.dci.2016.01.009_bib62","doi-asserted-by":"crossref","first-page":"18615","DOI":"10.3390\/ijms140918615","article-title":"Molecular cloning and functional analysis of the duck TLR4 gene","volume":"14","author":"Zhao","year":"2013","journal-title":"Int. Mol. Sci."}],"container-title":["Developmental & Comparative Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0145305X1630009X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0145305X1630009X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T01:34:48Z","timestamp":1758936888000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0145305X1630009X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6]]},"references-count":62,"alternative-id":["S0145305X1630009X"],"URL":"https:\/\/doi.org\/10.1016\/j.dci.2016.01.009","relation":{},"ISSN":["0145-305X"],"issn-type":[{"value":"0145-305X","type":"print"}],"subject":[],"published":{"date-parts":[[2016,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular cloning and expression of toll-like receptor 4 (tlr4) in the blunt snout bream (Megalobrama amblycephala)","name":"articletitle","label":"Article Title"},{"value":"Developmental & Comparative Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.dci.2016.01.009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2016 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}