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Biol."},{"issue":"3","key":"10.1016\/j.dci.2025.105458_bib64","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1007\/s10695-022-01065-5","article-title":"An established kidney cell line from humpback grouper (Cromileptes altivelis) and its susceptibility to bacteria and heavy metals","volume":"48","author":"Wei","year":"2022","journal-title":"Fish Physiol. Biochem."},{"key":"10.1016\/j.dci.2025.105458_bib65","article-title":"Identification, genomic analysis, and pathogenicity of a novel highly virulent strain of infectious spleen and kidney necrosis virus isolated from the humpback grouper","volume":"599","author":"Wei","year":"2025","journal-title":"Cromileptes altivelis. 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