{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T05:01:32Z","timestamp":1784869292309,"version":"3.55.0"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","award":["RS-2019-NR040075"],"award-info":[{"award-number":["RS-2019-NR040075"]}],"id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","award":["RS-2022-NR075052"],"award-info":[{"award-number":["RS-2022-NR075052"]}],"id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015499","name":"Jeonbuk National University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100015499","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Developmental &amp; Comparative Immunology"],"published-print":{"date-parts":[[2026,7]]},"DOI":"10.1016\/j.dci.2026.105633","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T05:44:03Z","timestamp":1779428643000},"page":"105633","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Mutations of the lon protease and tryptophan operon gene trpA in Edwardsiella tarda regulate colonization and pathogenesis in olive flounder fish"],"prefix":"10.1016","volume":"180","author":[{"given":"Jun","family":"Kwon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal","family":"Senevirathne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Woo","family":"Jun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2028-2207","authenticated-orcid":false,"given":"John Hwa","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.dci.2026.105633_bib1","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/s13567-022-01096-z","article-title":"Safety assessment of compliant, highly invasive, lipid A-altered, O-antigen-defected Salmonella strains as prospective vaccine delivery systems","volume":"53","author":"Aganja","year":"2022","journal-title":"Vet. Res."},{"key":"10.1016\/j.dci.2026.105633_bib2","doi-asserted-by":"crossref","first-page":"32","DOI":"10.3389\/fbioe.2018.00032","article-title":"Metabolic engineering of the shikimate pathway for production of aromatics and derived compounds\u2014present and future strain construction strategies","volume":"6","author":"Averesch","year":"2018","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.dci.2026.105633_bib4","series-title":"Global Regulation of the Lon Protease of Pseudomonas aeruginosa and Its Influence on Ciprofloxacin Resistance and Virulence","author":"Breidenstein","year":"2012"},{"key":"10.1016\/j.dci.2026.105633_bib3","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0049123","article-title":"The Lon protease is essential for full virulence in Pseudomonas aeruginosa","volume":"7","author":"Breidenstein","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.dci.2026.105633_bib5","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.fsi.2011.03.006","article-title":"Generation of two auxotrophic genes knock-out Edwardsiella tarda and assessment of its potential as a combined vaccine in olive flounder (Paralichthys olivaceus)","volume":"31","author":"Choi","year":"2011","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.dci.2026.105633_bib6","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1099\/jmm.0.47778-0","article-title":"The Lon protease regulates swarming motility and virulence gene expression in Proteus mirabilis","volume":"57","author":"Clemmer","year":"2008","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.dci.2026.105633_bib7","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1128\/aem.62.3.848-852.1996","article-title":"Development of a defined minimal medium for the growth of Edwardsiella ictaluri","volume":"62","author":"Collins","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.dci.2026.105633_bib8","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00740-19","article-title":"Twitching and swimming motility play a role in Ralstonia solanacearum pathogenicity","volume":"5","author":"Corral","year":"2020","journal-title":"mSphere"},{"key":"10.1016\/j.dci.2026.105633_bib9","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1111\/jam.12198","article-title":"Invasin of Edwardsiella tarda is essential for its haemolytic activity, biofilm formation and virulence towards fish","volume":"115","author":"Dong","year":"2013","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.dci.2026.105633_bib10","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a000299","article-title":"Bacterial nanomachines: the flagellum and type III injectisome","volume":"2","author":"Erhardt","year":"2010","journal-title":"Cold Spring Harbor Perspect. Biol."},{"key":"10.1016\/j.dci.2026.105633_bib11","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1038\/nm0396-334","article-title":"Auxotrophic vaccines for tuberculosis","volume":"2","author":"Guleria","year":"1996","journal-title":"Nat. Med."},{"key":"10.1016\/j.dci.2026.105633_bib12","series-title":"FliC, a Flagellin Protein, is Essential for the Growth and Virulence of Fish Pathogen Edwardsiella tarda","author":"He","year":"2012"},{"key":"10.1016\/j.dci.2026.105633_bib13","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1007\/s12088-011-0142-1","article-title":"Indole affects biofilm formation in bacteria","volume":"50","author":"Hu","year":"2010","journal-title":"Indian J. Microbiol."},{"key":"10.1016\/j.dci.2026.105633_bib14","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.3390\/microorganisms12051031","article-title":"Expanding the spectrum of diseases and disease associations caused by Edwardsiella tarda and related species","volume":"12","author":"Janda","year":"2024","journal-title":"Microorganisms"},{"key":"10.1016\/j.dci.2026.105633_bib15","doi-asserted-by":"crossref","first-page":"6284","DOI":"10.1038\/s41598-019-42681-1","article-title":"Isolation and characterisation of pVa-21, a giant bacteriophage with anti-biofilm potential against Vibrio alginolyticus","volume":"9","author":"Kim","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.dci.2026.105633_bib16","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1080\/19490976.2020.1777923","article-title":"Deletion of the lon gene augments expression of Salmonella Pathogenicity Island (SPI)-1 and metal ion uptake genes leading to the accumulation of bactericidal hydroxyl radicals and host pro-inflammatory cytokine-mediated rapid intracellular clearance","volume":"11","author":"Kirthika","year":"2020","journal-title":"Gut Microbes"},{"key":"10.1016\/j.dci.2026.105633_bib17","doi-asserted-by":"crossref","first-page":"9056","DOI":"10.3390\/ijms25169056","article-title":"Intracellular survival and pathogenicity modulation of Salmonella Lon, CpxR, and RfaL mutants used as live bacterial vectors under abiotic stress, unveiling the link between stress response and virulence in epithelial cells","volume":"25","author":"Kirthika","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.dci.2026.105633_bib18","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1038\/nbt.1635","article-title":"Rationalizing the development of live attenuated virus vaccines","volume":"28","author":"Lauring","year":"2010","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.dci.2026.105633_bib19","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.tim.2015.08.001","article-title":"Roles of indole as an interspecies and interkingdom signaling molecule","volume":"23","author":"Lee","year":"2015","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.dci.2026.105633_bib20","doi-asserted-by":"crossref","first-page":"940","DOI":"10.3390\/vaccines3040940","article-title":"Live-Attenuated bacterial vectors: tools for vaccine and therapeutic agent delivery","volume":"3","author":"Lin","year":"2015","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.dci.2026.105633_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.micinf.2022.104955","article-title":"AroC, a chorismate synthase, is required for the formation of Edwardsiella tarda biofilms","volume":"24","author":"Liu","year":"2022","journal-title":"Microb. Infect."},{"key":"10.1016\/j.dci.2026.105633_bib22","doi-asserted-by":"crossref","first-page":"464","DOI":"10.3390\/pathogens11040464","article-title":"The involvement of thiamine uptake in the virulence of Edwardsiella piscicida","volume":"11","author":"Liu","year":"2022","journal-title":"Pathogens"},{"key":"10.1016\/j.dci.2026.105633_bib23","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.mib.2006.02.001","article-title":"Regulation of flagella","volume":"9","author":"McCarter","year":"2006","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.dci.2026.105633_bib24","series-title":"Adaptor-Mediated Lon Proteolysis Restricts Bacillus subtilis Hyperflagellation","first-page":"250","volume":"112","author":"Mukherjee","year":"2015"},{"key":"10.1016\/j.dci.2026.105633_bib25","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1186\/s12866-025-04232-9","article-title":"Presence of virulent Edwardsiella tarda in farmed nile tilapia and striped catfish","volume":"25","author":"Okasha","year":"2025","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.dci.2026.105633_bib26","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1038\/nrmicro1493","article-title":"From the origin of species to the origin of bacterial flagella","volume":"4","author":"Pallen","year":"2006","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.dci.2026.105633_bib27","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1016\/j.dci.2011.03.007","article-title":"Advances in fish vaccine delivery","volume":"35","author":"Plant","year":"2011","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.dci.2026.105633_bib28","doi-asserted-by":"crossref","DOI":"10.5772\/53504","article-title":"Fish cytokines and immune response","volume":"1","author":"Reyes-Cerpa","year":"2012","journal-title":"New Adv. Contribut. Fish Biol."},{"key":"10.1016\/j.dci.2026.105633_bib29","doi-asserted-by":"crossref","first-page":"3155","DOI":"10.1128\/JB.00053-08","article-title":"Aro mutations in Salmonella enterica cause defects in cell wall and outer membrane integrity","volume":"190","author":"Sebkova","year":"2008","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.dci.2026.105633_bib30","series-title":"Motility Test Medium Protocol","first-page":"215","volume":"214","author":"Shields","year":"2011"},{"key":"10.1016\/j.dci.2026.105633_bib31","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1577\/1548-8667(1995)007<0292:TOEPAI>2.3.CO;2","article-title":"Toxicity of extracellular products and intracellular components of Edwardsiella tarda in the Japanese eel and flounder","volume":"7","author":"Suprapto","year":"1995","journal-title":"J. Aquat. Anim. Health"},{"key":"10.1016\/j.dci.2026.105633_bib32","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1128\/JB.184.1.224-232.2002","article-title":"The ATP-dependent lon protease of Salmonella enterica serovar Typhimurium regulates invasion and expression of genes carried on Salmonella pathogenicity island 1","volume":"184","author":"Takaya","year":"2002","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.dci.2026.105633_bib33","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1111\/j.1365-2761.2005.00646.x","article-title":"Potential use of a Yersinia ruckeri O1 auxotrophic aroA mutant as a live attenuated vaccine","volume":"28","author":"Temprano","year":"2005","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.dci.2026.105633_bib34","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1128\/jb.31.6.575-580.1936","article-title":"The use of semi-solid agar for the detection of bacterial motility","volume":"31","author":"Tittsler","year":"1936","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.dci.2026.105633_bib35","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/S1050-4648(03)00078-0","article-title":"The auxotrophic aroA mutant of Aeromonas hydrophila as a live attenuated vaccine against A. salmonicida infections in rainbow trout (Oncorhynchus mykiss)","volume":"16","author":"Vivas","year":"2004","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.dci.2026.105633_bib36","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.vetmic.2012.12.027","article-title":"Edwardsiella tarda invasion of fish cell lines and the activation of divergent cell death pathways","volume":"163","author":"Wang","year":"2013","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.dci.2026.105633_bib37","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.aquaculture.2013.12.001","article-title":"Edwardsiella tarda: an intriguing problem in aquaculture","volume":"431","author":"Xu","year":"2014","journal-title":"Aquaculture"},{"key":"10.1016\/j.dci.2026.105633_bib38","doi-asserted-by":"crossref","first-page":"1324","DOI":"10.1128\/JB.184.5.1324-1334.2002","article-title":"YplA is exported by the Ysc, Ysa, and flagellar type III secretion systems of Yersinia enterocolitica","volume":"184","author":"Young","year":"2002","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.dci.2026.105633_bib39","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3390\/biology5020023","article-title":"The function of fish cytokines","volume":"5","author":"Zou","year":"2016","journal-title":"Biology"}],"container-title":["Developmental &amp; Comparative Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0145305X26000893?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0145305X26000893?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T04:18:16Z","timestamp":1784866696000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0145305X26000893"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":39,"alternative-id":["S0145305X26000893"],"URL":"https:\/\/doi.org\/10.1016\/j.dci.2026.105633","relation":{},"ISSN":["0145-305X"],"issn-type":[{"value":"0145-305X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Mutations of the lon protease and tryptophan operon gene trpA in Edwardsiella tarda regulate colonization and pathogenesis in olive flounder fish","name":"articletitle","label":"Article Title"},{"value":"Developmental & Comparative Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.dci.2026.105633","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"105633"}}