{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T04:24:43Z","timestamp":1787199883761,"version":"build-2736575974"},"reference-count":53,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2018-05942"],"award-info":[{"award-number":["RGPIN-2018-05942"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010784","name":"Ocean Frontier Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010784","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010785","name":"Canada First Research Excellence Fund","doi-asserted-by":"publisher","award":["sub-module J3"],"award-info":[{"award-number":["sub-module J3"]}],"id":[{"id":"10.13039\/501100010785","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Developmental & Comparative Immunology"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.dci.2026.105700","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T15:41:22Z","timestamp":1785944482000},"page":"105700","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Innate immune response of lumpfish (Cyclopterus lumpus) to oil-based and water-soluble adjuvants"],"prefix":"10.1016","volume":"182","author":[{"given":"Chukwu-Osazuwa","family":"Joy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Valderrama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trung","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Vasquez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajarooba","family":"Gnanagobal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danny","family":"Boyce","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer R.","family":"Hall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew L.","family":"Rise","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7701-3147","authenticated-orcid":false,"given":"Javier","family":"Santander","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.dci.2026.105700_bib2","first-page":"401","article-title":"Efficacy of intranasal vaccination with Moraxella spp antigens plus growth medium against bovine pinkeye","author":"Angelos","year":"2020","journal-title":"Annual Conference of Research Workers in Animal Diseases (CRWAD)"},{"issue":"1","key":"10.1016\/j.dci.2026.105700_bib3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13567-018-0620-0","article-title":"CD4: a vital player in the teleost fish immune system","volume":"50","author":"Ashfaq","year":"2019","journal-title":"Vet. Res."},{"key":"10.1016\/j.dci.2026.105700_bib4","doi-asserted-by":"crossref","DOI":"10.1155\/2018\/5432497","article-title":"Maintenance of fish health in aquaculture: review of epidemiological approaches for prevention and control of infectious disease of fish","volume":"2018","author":"Assefa","year":"2018","journal-title":"Vet. Med. Int."},{"issue":"1","key":"10.1016\/j.dci.2026.105700_bib5","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1186\/s12864-021-07464-x","article-title":"The transcriptomic responses of Atlantic salmon (Salmo salar) to high temperature stress alone, and in combination with moderate hypoxia","volume":"22","author":"Beemelmanns","year":"2021","journal-title":"BMC Genom."},{"issue":"8","key":"10.1016\/j.dci.2026.105700_bib6","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1093\/bioinformatics\/btp101","article-title":"ClueGO: a Cytoscape plug-in to decipher functionally grouped gene ontology and pathway annotation networks","volume":"25","author":"Bindea","year":"2009","journal-title":"Bioinformatics"},{"issue":"4","key":"10.1016\/j.dci.2026.105700_bib8","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clin. Chem."},{"issue":"5","key":"10.1016\/j.dci.2026.105700_bib9","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1097\/COH.0b013e32833d2cdb","article-title":"Role of adjuvants in modeling the immune response","volume":"5","author":"Carter","year":"2010","journal-title":"Curr. Opin. HIV AIDS"},{"issue":"11","key":"10.1016\/j.dci.2026.105700_bib10","doi-asserted-by":"crossref","first-page":"2113","DOI":"10.3390\/microorganisms10112113","article-title":"Multi-Organ transcriptome response of lumpfish (Cyclopterus lumpus) to Aeromonas salmonicida subspecies salmonicida systemic infection","volume":"10","author":"Chakraborty","year":"2022","journal-title":"Microorganisms"},{"issue":"4","key":"10.1016\/j.dci.2026.105700_bib11","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1016\/j.immuni.2010.10.002","article-title":"Vaccine adjuvants: putting innate immunity to work","volume":"33","author":"Coffman","year":"2010","journal-title":"Immunity"},{"issue":"4","key":"10.1016\/j.dci.2026.105700_bib12","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1037\/h0026256","article-title":"Weighted kappa: nominal scale agreement provision for scaled disagreement or partial credit","volume":"70","author":"Cohen","year":"1968","journal-title":"Psychol. Bull."},{"issue":"11","key":"10.1016\/j.dci.2026.105700_bib13","doi-asserted-by":"crossref","first-page":"3270","DOI":"10.4161\/21645515.2014.979640","article-title":"Mechanisms and pathways of innate immune activation and regulation in health and cancer","volume":"10","author":"Cui","year":"2014","journal-title":"Hum. Vaccines Immunother."},{"issue":"8","key":"10.1016\/j.dci.2026.105700_bib14","doi-asserted-by":"crossref","first-page":"819","DOI":"10.3390\/vaccines9080819","article-title":"Oral immunization of larvae and juvenile of lumpfish (Cyclopterus lumpus) against Vibrio anguillarum does not influence systemic immunity","volume":"9","author":"Dang","year":"2021","journal-title":"Vaccines"},{"key":"10.1016\/j.dci.2026.105700_bib15","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2022.1040336","article-title":"Current status and development prospects of aquatic vaccines","volume":"13","author":"Du","year":"2022","journal-title":"Front. Immunol."},{"key":"10.1016\/j.dci.2026.105700_bib16","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2024.1439465","article-title":"Transcriptomic response of lumpfish (Cyclopterus lumpus) head kidney to viral mimic, with a focus on the interferon regulatory factor family","volume":"15","author":"Emam","year":"2024","journal-title":"Front. Immunol."},{"key":"10.1016\/j.dci.2026.105700_bib17","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2020.567838","article-title":"Transcriptomic profiling of the adaptive and innate immune responses of Atlantic salmon to Renibacterium salmoninarum infection","volume":"11","author":"Eslamloo","year":"2020","journal-title":"Front. Immunol."},{"issue":"19","key":"10.1016\/j.dci.2026.105700_bib18","doi-asserted-by":"crossref","first-page":"3047","DOI":"10.1093\/bioinformatics\/btw354","article-title":"MultiQC: summarize analysis results for multiple tools and samples in a single report","volume":"32","author":"Ewels","year":"2016","journal-title":"Bioinformatics"},{"issue":"5","key":"10.1016\/j.dci.2026.105700_bib19","article-title":"An overview of vaccine adjuvants: current evidence and future perspectives","volume":"10","author":"Facciol\u00e0","year":"2022","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.dci.2026.105700_bib21","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.fsi.2018.02.047","article-title":"Aluminum adjuvant potentiates gilthead seabream immune responses but induces toxicity in splenic melanomacrophage centers","volume":"85","author":"Galindo-Villegas","year":"2019","journal-title":"Fish Shellfish Immunol."},{"issue":"4","key":"10.1016\/j.dci.2026.105700_bib20","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1016\/j.fsi.2013.07.046","article-title":"Recombinant TNF\u03b1 as oral vaccine adjuvant protects European sea bass against vibriosis: insights into the role of the CCL25\/CCR9 axis","volume":"35","author":"Galindo-Villegas","year":"2013","journal-title":"Fish Shellfish Immunol."},{"issue":"19","key":"10.1016\/j.dci.2026.105700_bib22","doi-asserted-by":"crossref","first-page":"2188","DOI":"10.1016\/j.vaccine.2016.03.025","article-title":"Sterile inflammation induced by Carbopol elicits robust adaptive immune responses in the absence of pathogen-associated molecular patterns","volume":"34","author":"Gartlan","year":"2016","journal-title":"Vaccine"},{"issue":"8","key":"10.1016\/j.dci.2026.105700_bib23","doi-asserted-by":"crossref","first-page":"2628","DOI":"10.1093\/bioinformatics\/btz931","article-title":"ShinyGO: a graphical gene-set enrichment tool for animals and plants","volume":"36","author":"Ge","year":"2020","journal-title":"Bioinformatics"},{"key":"10.1016\/j.dci.2026.105700_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2024.105165","article-title":"Transcriptome profiling of lumpfish (Cyclopterus lumpus) head kidney to Renibacterium salmoninarum at early and chronic infection stages","volume":"156","author":"Gnanagobal","year":"2024","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.dci.2026.105700_bib25","series-title":"Carbopol and its Pharmaceutical Significance: a Review. Pharmaceutical Reviews","author":"Hosmani","year":"2006"},{"issue":"2","key":"10.1016\/j.dci.2026.105700_bib26","doi-asserted-by":"crossref","DOI":"10.1002\/eji.202350512","article-title":"Rational design of polymer-based particulate vaccine adjuvants","volume":"54","author":"Huete-Carrasco","year":"2024","journal-title":"Eur. J. Immunol."},{"issue":"7","key":"10.1016\/j.dci.2026.105700_bib27","doi-asserted-by":"crossref","first-page":"1832","DOI":"10.1016\/j.vaccine.2009.11.083","article-title":"Comparative study of the effects of aluminum adjuvants and Freund's incomplete adjuvant on the immune response to an Edwardsiella tarda major antigen","volume":"28","author":"Jiao","year":"2010","journal-title":"Vaccine"},{"issue":"11","key":"10.1016\/j.dci.2026.105700_bib29","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.1093\/bioinformatics\/bty036","article-title":"Primer3_masker: integrating masking of template sequence with primer design software","volume":"34","author":"K\u00f5ressaar","year":"2018","journal-title":"Bioinformatics"},{"issue":"10","key":"10.1016\/j.dci.2026.105700_bib28","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1093\/bioinformatics\/btm091","article-title":"Enhancements and modifications of primer design program Primer3","volume":"23","author":"Koressaar","year":"2007","journal-title":"Bioinformatics"},{"issue":"4","key":"10.1016\/j.dci.2026.105700_bib30","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0035083","article-title":"Mixed adjuvant formulations reveal a new combination that elicit antibody response comparable to Freund's adjuvants","volume":"7","author":"Lai","year":"2012","journal-title":"PLoS One"},{"issue":"2","key":"10.1016\/j.dci.2026.105700_bib31","article-title":"Molecular actors of inflammation and their signaling pathways: mechanistic insights from zebrafish","volume":"12","author":"Leiba","year":"2023","journal-title":"Biology (Basel)."},{"issue":"4","key":"10.1016\/j.dci.2026.105700_bib32","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/bioinformatics\/btp692","article-title":"RNA-seq gene expression estimation with read mapping uncertainty","volume":"26","author":"Li","year":"2010","journal-title":"Bioinformatics"},{"issue":"11","key":"10.1016\/j.dci.2026.105700_bib33","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms7110569","article-title":"A review of fish vaccine development strategies: conventional methods and modern biotechnological approaches","volume":"7","author":"Ma","year":"2019","journal-title":"Microorganisms"},{"issue":"4","key":"10.1016\/j.dci.2026.105700_bib34","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/s10126-010-9279-x","article-title":"Immunological control of fish diseases","volume":"12","author":"Magnadottir","year":"2010","journal-title":"Mar. Biotechnol."},{"issue":"11","key":"10.1016\/j.dci.2026.105700_bib35","article-title":"Advancements in vaccine adjuvants: the journey from Alum to Nano formulations","volume":"11","author":"Moni","year":"2023","journal-title":"Vaccines (Basel)."},{"issue":"1","key":"10.1016\/j.dci.2026.105700_bib36","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1186\/s12864-018-4637-6","article-title":"Comparison of normalization methods for the analysis of metagenomic gene abundance data","volume":"19","author":"Pereira","year":"2018","journal-title":"BMC Genom."},{"issue":"5","key":"10.1016\/j.dci.2026.105700_bib37","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1111\/j.0818-9641.2004.01272.x","article-title":"Vaccine adjuvants: current state and future trends","volume":"82","author":"Petrovsky","year":"2004","journal-title":"Immunol. Cell Biol."},{"issue":"9","key":"10.1016\/j.dci.2026.105700_bib38","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res."},{"issue":"6","key":"10.1016\/j.dci.2026.105700_bib39","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1038\/s41573-021-00163-y","article-title":"Emerging concepts in the science of vaccine adjuvants","volume":"20","author":"Pulendran","year":"2021","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.dci.2026.105700_bib40","series-title":"Fish Immune System and Vaccines","first-page":"231","article-title":"Adjuvants for fish vaccines","author":"Raman","year":"2022"},{"issue":"12","key":"10.1016\/j.dci.2026.105700_bib41","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1038\/nm.3409","article-title":"Key roles of adjuvants in modern vaccines","volume":"19","author":"Reed","year":"2013","journal-title":"Nat. Med."},{"key":"10.1016\/j.dci.2026.105700_bib42","series-title":"Vaccine Adjuvants, Methods in Molecular Biology Davies G","article-title":"Immunology of vaccine adjuvants","author":"Ribeiro","year":"2010"},{"issue":"1","key":"10.1016\/j.dci.2026.105700_bib43","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: a bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1016\/j.dci.2026.105700_bib44","series-title":"Molecular Cloning: a Laboratory Manual","author":"Sambrook","year":"2001"},{"issue":"1\u20133","key":"10.1016\/j.dci.2026.105700_bib45","first-page":"75","article-title":"Induction and direction of immune responses by vaccine adjuvants","volume":"21","author":"Schijns","year":"2001","journal-title":"Crit. Rev. Immunol."},{"issue":"11","key":"10.1016\/j.dci.2026.105700_bib46","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: a software environment for integrated models of biomolecular interaction networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Res."},{"key":"10.1016\/j.dci.2026.105700_bib47","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1093\/ilar.46.3.280","article-title":"Adjuvants and antibody production: dispelling the myths associated with freund's complete and other adjuvants","volume":"46","author":"Stils","year":"2005","journal-title":"ILAR J."},{"issue":"6","key":"10.1016\/j.dci.2026.105700_bib48","doi-asserted-by":"crossref","first-page":"1740","DOI":"10.1016\/j.fsi.2013.02.029","article-title":"Adjuvants and immunostimulants in fish vaccines: current knowledge and future perspectives","volume":"35","author":"Tafalla","year":"2013","journal-title":"Fish Shellfish Immunol."},{"issue":"1","key":"10.1016\/j.dci.2026.105700_bib49","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1186\/s13059-016-1060-7","article-title":"Erratum to: a benchmark for RNA-seq quantification pipelines","volume":"17","author":"Teng","year":"2016","journal-title":"Genome Biol."},{"issue":"15","key":"10.1016\/j.dci.2026.105700_bib50","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gks596","article-title":"Primer3--new capabilities and interfaces","volume":"40","author":"Untergasser","year":"2012","journal-title":"Nucleic Acids Res."},{"issue":"7","key":"10.1016\/j.dci.2026.105700_bib51","doi-asserted-by":"crossref","DOI":"10.1186\/gb-2002-3-7-research0034","article-title":"Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes","volume":"3","author":"Vandesompele","year":"2002","journal-title":"Genome Biol."},{"issue":"4","key":"10.1016\/j.dci.2026.105700_bib52","doi-asserted-by":"crossref","DOI":"10.1096\/fj.202002393RR","article-title":"Fish NF-\u03baB couples TCR and IL-17 signals to regulate ancestral T-cell immune response against bacterial infection","volume":"35","author":"Wei","year":"2021","journal-title":"FASEB J."},{"key":"10.1016\/j.dci.2026.105700_bib53","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.789465","article-title":"Transcriptome profiling of Atlantic salmon (Salmo salar) parr with higher and lower pathogen loads following Piscirickettsia salmonis infection","volume":"12","author":"Xue","year":"2021","journal-title":"Front. Immunol."},{"issue":"1","key":"10.1016\/j.dci.2026.105700_bib54","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1038\/s41392-024-01868-3","article-title":"Targeting cytokine and chemokine signaling pathways for cancer therapy","volume":"9","author":"Yi","year":"2024","journal-title":"Signal Transduct. Targeted Ther."},{"issue":"2","key":"10.1016\/j.dci.2026.105700_bib55","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1111\/cei.12841","article-title":"The genetic background influences the cellular and humoral immune responses to vaccines","volume":"186","author":"Zeng","year":"2016","journal-title":"Clin. Exp. Immunol."}],"container-title":["Developmental & Comparative Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0145305X26001564?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0145305X26001564?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T03:57:06Z","timestamp":1787198226000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0145305X26001564"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":53,"alternative-id":["S0145305X26001564"],"URL":"https:\/\/doi.org\/10.1016\/j.dci.2026.105700","relation":{},"ISSN":["0145-305X"],"issn-type":[{"value":"0145-305X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Innate immune response of lumpfish (Cyclopterus lumpus) to oil-based and water-soluble adjuvants","name":"articletitle","label":"Article Title"},{"value":"Developmental & Comparative Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.dci.2026.105700","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Crown Copyright \u00a9 2026 Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"105700"}}