{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T09:05:56Z","timestamp":1789635956626,"version":"4.0.0"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100013805","name":"Shanxi Agricultural University","doi-asserted-by":"publisher","award":["YZGC133"],"award-info":[{"award-number":["YZGC133"]}],"id":[{"id":"10.13039\/501100013805","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009997","name":"Earmarked Fund for Modern Agro-industry Technology Research System","doi-asserted-by":"publisher","award":["2024QT155"],"award-info":[{"award-number":["2024QT155"]}],"id":[{"id":"10.13039\/501100009997","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012326","name":"International Science and Technology Cooperation Programme","doi-asserted-by":"publisher","award":["SQ2023YFE0102235"],"award-info":[{"award-number":["SQ2023YFE0102235"]}],"id":[{"id":"10.13039\/501100012326","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Developmental & Comparative Immunology"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.dci.2026.105714","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T23:02:10Z","timestamp":1787266930000},"page":"105714","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Pathogen recognition and anti-infective immune responses mediated by TLR22 in largemouth bass Micropterus salmoides"],"prefix":"10.1016","volume":"183","author":[{"given":"Ke","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling yun","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2925-6563","authenticated-orcid":false,"given":"Yu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaozhen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianzong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.dci.2026.105714_bib1","doi-asserted-by":"crossref","first-page":"7671","DOI":"10.1007\/s10499-024-01535-y","article-title":"Aquaculture and Aeromonas hydrophila: a complex interplay of environmental factors and virulence","volume":"32","author":"Abdella","year":"2024","journal-title":"Aquac. Int."},{"issue":"7","key":"10.1016\/j.dci.2026.105714_bib2","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1038\/nri1391","article-title":"Toll-like receptor signalling","volume":"4","author":"Akira","year":"2004","journal-title":"Nat. Rev. Immunol."},{"issue":"W1","key":"10.1016\/j.dci.2026.105714_bib3","doi-asserted-by":"crossref","first-page":"W39","DOI":"10.1093\/nar\/gkv416","article-title":"The MEME suite","volume":"43","author":"Bailey","year":"2015","journal-title":"Nucleic Acids Res."},{"issue":"8\u20139","key":"10.1016\/j.dci.2026.105714_bib4","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/S0145-305X(01)00033-7","article-title":"The acute phase response and innate immunity of fish","volume":"25","author":"Bayne","year":"2001","journal-title":"Dev. Comp. Immunol."},{"issue":"1","key":"10.1016\/j.dci.2026.105714_bib5","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s00251-020-01196-0","article-title":"Anatomy of teleost fish immune structures and organs","volume":"73","author":"Bj\u00f8rgen","year":"2021","journal-title":"Immunogenetics"},{"issue":"4","key":"10.1016\/j.dci.2026.105714_bib6","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.str.2011.02.004","article-title":"The structural biology of toll-like receptors","volume":"19","author":"Botos","year":"2011","journal-title":"Structure"},{"issue":"15\u201316","key":"10.1016\/j.dci.2026.105714_bib7","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1016\/j.molimm.2011.05.027","article-title":"Bioactivity studies of rainbow trout (Oncorhynchus mykiss) interleukin-6: effects on macrophage growth and antimicrobial peptide gene expression","volume":"48","author":"Costa","year":"2011","journal-title":"Mol. Immunol."},{"issue":"3","key":"10.1016\/j.dci.2026.105714_bib8","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/j.fsi.2012.05.034","article-title":"Orange-spotted grouper (Epinephelus coioides) toll-like receptor 22: molecular characterization, expression pattern and pertinent signaling pathways","volume":"33","author":"Ding","year":"2012","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.dci.2026.105714_bib9","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1016\/j.fsi.2019.08.042","article-title":"Multiple subtypes of TLR22 molecule from Schizothorax prenanti present the functional diversity in ligand recognition and signal activation","volume":"93","author":"Du","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.dci.2026.105714_bib10","series-title":"The Proteomics Protocols Handbook","first-page":"571","article-title":"Protein identification and analysis tools on the ExPASy server","author":"Gasteiger","year":"2005"},{"issue":"6","key":"10.1016\/j.dci.2026.105714_bib11","doi-asserted-by":"crossref","first-page":"1729","DOI":"10.1016\/j.fsi.2013.09.032","article-title":"The mucosal immune system of fish: the evolution of tolerating commensals while fighting pathogens","volume":"35","author":"Gomez","year":"2013","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.dci.2026.105714_bib13","article-title":"Characterization of TLR1 and expression profiling of TLR signaling pathway related genes in response to Aeromonas hydrophila challenge in hybrid yellow catfish (Pelteobagrus fulvidraco \u2640 \u00d7 P. vachelli \u2642)","volume":"14","author":"Guo","year":"2023","journal-title":"Front. Immunol."},{"issue":"2","key":"10.1016\/j.dci.2026.105714_bib12","doi-asserted-by":"crossref","first-page":"288","DOI":"10.3390\/ani13020288","article-title":"Toll-like receptor 3 in the hybrid yellow catfish (Pelteobagrus fulvidraco \u2640 \u00d7 P. vachelli \u2642): protein structure, evolution and immune response to exogenous Aeromonas hydrophila and poly(I:C) stimuli","volume":"13","author":"Guo","year":"2023","journal-title":"Animals"},{"issue":"6","key":"10.1016\/j.dci.2026.105714_bib14","doi-asserted-by":"crossref","first-page":"3329","DOI":"10.1128\/IAI.71.6.3329-3336.2003","article-title":"Study of capsular polysaccharide from Vibrio parahaemolyticus","volume":"71","author":"Hsieh","year":"2003","journal-title":"Infect. Immun."},{"issue":"Pt 1","key":"10.1016\/j.dci.2026.105714_bib15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1101\/SQB.1989.054.01.003","article-title":"Approaching the asymptote? Evolution and revolution in immunology","volume":"54","author":"Janeway","year":"1989","journal-title":"Cold Spring Harbor Symp. Quant. Biol."},{"key":"10.1016\/j.dci.2026.105714_bib16","doi-asserted-by":"crossref","first-page":"3003","DOI":"10.3389\/fimmu.2019.03003","article-title":"Thoroughly remold the localization and signaling pathway of TLR22","volume":"10","author":"Ji","year":"2020","journal-title":"Front. Immunol."},{"key":"10.1016\/j.dci.2026.105714_bib17","doi-asserted-by":"crossref","first-page":"461","DOI":"10.3389\/fimmu.2014.00461","article-title":"Toll-like receptor signaling pathways","volume":"5","author":"Kawasaki","year":"2014","journal-title":"Front. Immunol."},{"key":"10.1016\/j.dci.2026.105714_bib19","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.molimm.2021.06.025","article-title":"TLR22-mediated activation of TNF-\u03b1-caspase-1\/IL-1\u03b2 inflammatory axis leads to apoptosis of Aeromonas hydrophila-infected macrophages","volume":"137","author":"Kumar","year":"2021","journal-title":"Mol. Immunol."},{"issue":"6","key":"10.1016\/j.dci.2026.105714_bib18","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"issue":"D1","key":"10.1016\/j.dci.2026.105714_bib20","doi-asserted-by":"crossref","first-page":"D493","DOI":"10.1093\/nar\/gkx922","article-title":"20 years of the SMART protein domain annotation resource","volume":"46","author":"Letunic","year":"2018","journal-title":"Nucleic Acids Res."},{"issue":"4","key":"10.1016\/j.dci.2026.105714_bib21","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2^-\u0394\u0394Ct method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"issue":"2","key":"10.1016\/j.dci.2026.105714_bib22","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.fsi.2004.09.006","article-title":"Innate immunity of fish (overview)","volume":"20","author":"Magnad\u00f3ttir","year":"2006","journal-title":"Fish Shellfish Immunol."},{"issue":"5","key":"10.1016\/j.dci.2026.105714_bib23","doi-asserted-by":"crossref","first-page":"3474","DOI":"10.4049\/jimmunol.181.5.3474","article-title":"Teleost TLR22 recognizes RNA duplex to induce IFN and protect cells from birnaviruses","volume":"181","author":"Matsuo","year":"2008","journal-title":"J. Immunol."},{"issue":"7164","key":"10.1016\/j.dci.2026.105714_bib24","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1038\/nature06246","article-title":"Recognition of microorganisms and activation of the immune response","volume":"449","author":"Medzhitov","year":"2007","journal-title":"Nature"},{"key":"10.1016\/j.dci.2026.105714_bib25","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.dci.2013.12.003","article-title":"Toll-like receptor 22 of gilthead seabream, Sparus aurata: molecular cloning, expression profiles and post-transcriptional regulation","volume":"44","author":"Mu\u00f1oz","year":"2014","journal-title":"Dev. Comp. Immunol."},{"issue":"12","key":"10.1016\/j.dci.2026.105714_bib26","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1016\/j.dci.2011.03.006","article-title":"Toll-like receptors in bony fish: from genomics to function","volume":"35","author":"Palti","year":"2011","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.dci.2026.105714_bib27","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.dci.2017.11.021","article-title":"Toll-like receptor (TLR) 22, a non-mammalian TLR in Asian seabass, Lates calcarifer: characterisation, ontogeny and inductive expression upon exposure with bacteria and ligands","volume":"81","author":"Paria","year":"2018","journal-title":"Dev. Comp. Immunol."},{"issue":"2","key":"10.1016\/j.dci.2026.105714_bib28","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.dci.2013.08.010","article-title":"Ligand specificities of toll-like receptors in fish: indications from infection studies","volume":"43","author":"Pietretti","year":"2014","journal-title":"Dev. Comp. Immunol."},{"issue":"1","key":"10.1016\/j.dci.2026.105714_bib29","first-page":"77","article-title":"Conservation of toll-like receptor signaling pathways in teleost fish","volume":"1","author":"Purcell","year":"2006","journal-title":"Comp. Biochem. Physiol., Part D: Genomics Proteomics"},{"key":"10.1016\/j.dci.2026.105714_bib30","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/j.fsi.2019.09.067","article-title":"Duplication of toll-like receptor 22 in teleost fishes","volume":"94","author":"Qi","year":"2019","journal-title":"Fish Shellfish Immunol."},{"issue":"3\u20134","key":"10.1016\/j.dci.2026.105714_bib31","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.vetimm.2009.09.021","article-title":"Toll-like receptor signaling in bony fish","volume":"134","author":"Rebl","year":"2010","journal-title":"Vet. Immunol. Immunopathol."},{"issue":"2","key":"10.1016\/j.dci.2026.105714_bib32","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.fsi.2015.06.020","article-title":"Molecular cloning and comparative responses of toll-like receptor 22 following ligands stimulation and parasitic infection in yellowtail (Seriola lalandi)","volume":"46","author":"Reyes-Becerril","year":"2015","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.dci.2026.105714_bib33","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.1016\/j.ijbiomac.2020.07.293","article-title":"Structure of fish toll-like receptors (TLR) and NOD-like receptors (NLR)","volume":"161","author":"Sahoo","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"issue":"3","key":"10.1016\/j.dci.2026.105714_bib34","doi-asserted-by":"crossref","first-page":"525","DOI":"10.3390\/biology4030525","article-title":"The mucosal immune system of teleost fish","volume":"4","author":"Salinas","year":"2015","journal-title":"Biology"},{"issue":"1","key":"10.1016\/j.dci.2026.105714_bib35","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1007\/s12010-014-1058-0","article-title":"Toll-like receptor 22 in Labeo rohita: molecular cloning, characterization, 3D modeling, and expression analysis following ligands stimulation and bacterial infection","volume":"174","author":"Samanta","year":"2014","journal-title":"Appl. Biochem. Biotechnol."},{"issue":"4","key":"10.1016\/j.dci.2026.105714_bib36","doi-asserted-by":"crossref","first-page":"1836","DOI":"10.4049\/jimmunol.0801755","article-title":"Evolution of lipopolysaccharide (LPS) recognition and signaling: fish TLR4 does not recognize LPS and negatively regulates NF-\u03baB activation","volume":"182","author":"Sepulcre","year":"2009","journal-title":"J. Immunol."},{"key":"10.1016\/j.dci.2026.105714_bib37","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1186\/1471-2148-12-256","article-title":"Diversification of the expanded teleost-specific toll-like receptor family in Atlantic cod, Gadus morhua","volume":"12","author":"Sundaram","year":"2012","journal-title":"BMC Evol. Biol."},{"key":"10.1016\/j.dci.2026.105714_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2023.108579","article-title":"Molecular characterization of a teleost-specific toll-like receptor 22 (tlr22) gene from yellow catfish (Pelteobagrus fulvidraco) and its transcriptional change in response to poly I:C and Aeromonas hydrophila stimuli","volume":"134","author":"Wei","year":"2023","journal-title":"Fish Shellfish Immunol."},{"issue":"12","key":"10.1016\/j.dci.2026.105714_bib39","doi-asserted-by":"crossref","first-page":"1724","DOI":"10.3390\/biology14121724","article-title":"Genome-wide identification, characterization, and expression profiles of TLR genes in darkbarbel catfish (Pelteobagrus vachelli) following Aeromonas hydrophila infection","volume":"14","author":"Wen","year":"2025","journal-title":"Biology"},{"issue":"1","key":"10.1016\/j.dci.2026.105714_bib40","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.fsi.2010.10.014","article-title":"Molecular characterization of a toll-like receptor 22 homologue in large yellow croaker (Pseudosciaena crocea) and promoter activity analysis of its 5\u2032-flanking sequence","volume":"30","author":"Xiao","year":"2011","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.dci.2026.105714_bib41","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.fsi.2017.05.025","article-title":"Characterization of toll-like receptor 22 in turbot (Scophthalmus maximus)","volume":"66","author":"Xing","year":"2017","journal-title":"Fish Shellfish Immunol."},{"issue":"2","key":"10.1016\/j.dci.2026.105714_bib42","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.fsi.2004.09.005","article-title":"Ontogeny of the immune system of fish","volume":"20","author":"Zapata","year":"2006","journal-title":"Fish Shellfish Immunol."},{"issue":"24","key":"10.1016\/j.dci.2026.105714_bib43","doi-asserted-by":"crossref","DOI":"10.3390\/ijms242417215","article-title":"IL-6 mutation attenuates liver injury caused by Aeromonas hydrophila infection by reducing oxidative stress in zebrafish","volume":"24","author":"Zhai","year":"2023","journal-title":"Int. J. Mol. Sci."},{"issue":"Pt 1","key":"10.1016\/j.dci.2026.105714_bib44","article-title":"Hepcidin contributes to largemouth bass (Micropterus salmoides) against bacterial infections","volume":"266","author":"Zhang","year":"2024","journal-title":"Int. J. Biol. Macromol."}],"container-title":["Developmental & Comparative Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0145305X26001709?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0145305X26001709?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T08:48:55Z","timestamp":1789634935000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0145305X26001709"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":44,"alternative-id":["S0145305X26001709"],"URL":"https:\/\/doi.org\/10.1016\/j.dci.2026.105714","relation":{},"ISSN":["0145-305X"],"issn-type":[{"value":"0145-305X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Pathogen recognition and anti-infective immune responses mediated by TLR22 in largemouth bass Micropterus salmoides","name":"articletitle","label":"Article Title"},{"value":"Developmental & Comparative Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.dci.2026.105714","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"105714"}}