{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T21:31:53Z","timestamp":1793309513452,"version":"4.5.0"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Developmental & Comparative Immunology"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.dci.2026.105753","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T06:18:33Z","timestamp":1792563513000},"page":"105753","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["In vitro inhibitory activity of recombinant olive flounder interferon-\u03b3 produced in Chlorella vulgaris against viral hemorrhagic septicemia virus infection"],"prefix":"10.1016","volume":"184","author":[{"given":"Min-Jeong","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su-Hyun","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4923-5121","authenticated-orcid":false,"given":"Tae-Jin","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.dci.2026.105753_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2025.110539","article-title":"Plant- and microalgae-made fish vaccines","volume":"165","author":"Angulo","year":"2025","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.dci.2026.105753_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2022.108456","article-title":"In vitro and in vivo evaluation of the antiviral activity of arctigenin, ribavirin, and ivermectin against viral hemorrhagic septicemia virus infection","volume":"132","author":"Baek","year":"2023","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.dci.2026.105753_bib3","doi-asserted-by":"crossref","first-page":"223","DOI":"10.3390\/synbio2020013","article-title":"Recombinant protein expression and its biotechnological applications in Chlorella spp","volume":"2","author":"Chen","year":"2024","journal-title":"SynBio"},{"key":"10.1016\/j.dci.2026.105753_bib4","doi-asserted-by":"crossref","first-page":"2694","DOI":"10.1016\/j.tibtech.2025.04.001","article-title":"Microalgae-based vaccines for aquaculture","volume":"43","author":"Chen","year":"2025","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.dci.2026.105753_bib5","doi-asserted-by":"crossref","first-page":"465","DOI":"10.4049\/jimmunol.1801376","article-title":"Viral resistance and IFN signaling in STAT2 knockout fish cells","volume":"203","author":"Dehler","year":"2019","journal-title":"J. Immunol."},{"key":"10.1016\/j.dci.2026.105753_bib6","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1111\/raq.12349","article-title":"Fish type I and type II interferons: composition, receptor usage, production and function","volume":"12","author":"Gan","year":"2020","journal-title":"Rev. Aquacult."},{"key":"10.1016\/j.dci.2026.105753_bib7","doi-asserted-by":"crossref","first-page":"23537","DOI":"10.1074\/jbc.M109.096925","article-title":"Comparison of macrophage antimicrobial responses induced by type II interferons of the goldfish (Carassius auratus L.)","volume":"285","author":"Grayfer","year":"2010","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.dci.2026.105753_bib8","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2020.571650","article-title":"Selective stimulation of duplicated Atlantic salmon MHC pathway genes by interferon-gamma","volume":"11","author":"Grimholt","year":"2020","journal-title":"Front. Immunol."},{"key":"10.1016\/j.dci.2026.105753_bib9","doi-asserted-by":"crossref","DOI":"10.1002\/edn3.70083","article-title":"Environmental RNA-based surveillance of viral hemorrhagic septicemia virus (VHSV) in olive flounder (Paralichthys olivaceus) aquaculture: detection dynamics and risk assessment","volume":"7","author":"Kang","year":"2025","journal-title":"Environ. DNA"},{"key":"10.1016\/j.dci.2026.105753_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.algal.2023.103146","article-title":"Enhancement of Chlorella transformation efficacy by insert fragmentation","volume":"72","author":"Kim","year":"2023","journal-title":"Algal Res."},{"key":"10.1016\/j.dci.2026.105753_bib14","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.3390\/vaccines11071205","article-title":"High-level expression of recombinant VHSV glycoprotein using transformed C. vulgaris and verification of vaccine efficacy","volume":"11","author":"Kim","year":"2023","journal-title":"Vaccines"},{"key":"10.1016\/j.dci.2026.105753_bib12","doi-asserted-by":"crossref","first-page":"25","DOI":"10.3354\/dao02394","article-title":"Generation and characterization of NV gene-knockout recombinant viral hemorrhagic septicemia virus (VHSV) genotype IVa","volume":"97","author":"Kim","year":"2011","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.dci.2026.105753_bib10","first-page":"1","article-title":"Genetic relationship of the VHSV (viral hemorrhagic septicemia virus) isolated from cultured olive flounder, Paralichthys olivaceus in Korea","volume":"16","author":"Kim","year":"2003","journal-title":"J. Fish Pathol."},{"key":"10.1016\/j.dci.2026.105753_bib11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.7847\/jfp.2011.24.1.001","article-title":"Genetic positioning of Korean viral hemorrhagic septicemia virus (VHSV) from cultured and wild marine fishes","volume":"24","author":"Kim","year":"2011","journal-title":"J. Fish Pathol."},{"key":"10.1016\/j.dci.2026.105753_bib15","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.aquaculture.2018.12.085","article-title":"Differential expression profile of innate immune genes in the liver of olive flounder (Paralichthys olivaceus) against viral haemorrhagic septicaemia virus (VHSV) at host susceptible and non-susceptible temperatures","volume":"503","author":"Kole","year":"2019","journal-title":"Aquaculture"},{"key":"10.1016\/j.dci.2026.105753_bib16","doi-asserted-by":"crossref","first-page":"379","DOI":"10.4490\/algae.2013.28.4.379","article-title":"Expression of bovine lactoferrin N-lobe by the green alga, Chlorella vulgaris","volume":"28","author":"Koo","year":"2013","journal-title":"ALGAE"},{"key":"10.1016\/j.dci.2026.105753_bib17","doi-asserted-by":"crossref","first-page":"10025","DOI":"10.1128\/JVI.01294-12","article-title":"Zebrafish ISG15 exerts a strong antiviral activity against RNA and DNA viruses and regulates the interferon response","volume":"87","author":"Langevin","year":"2013","journal-title":"J. Virol."},{"key":"10.1016\/j.dci.2026.105753_bib18","article-title":"Microalgal delivery of recombinant fish interferon modulates gut microbiota and enhances antiviral immunity in fish","volume":"215","author":"Liu","year":"2026","journal-title":"J. Immunol."},{"key":"10.1016\/j.dci.2026.105753_bib19","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2021.650820","article-title":"Efficient transient expression of recombinant proteins using DNA viral vectors in freshwater microalgal species","volume":"12","author":"Malla","year":"2021","journal-title":"Front. Plant Sci."},{"key":"10.1016\/j.dci.2026.105753_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2024.109767","article-title":"Antiviral effects of umbelliferone against viral hemorrhagic septicemia virus in olive flounder (Paralichthys olivaceus)","volume":"152","author":"Mendis","year":"2024","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.dci.2026.105753_bib21","doi-asserted-by":"crossref","first-page":"3427","DOI":"10.4238\/2011.October.21.1","article-title":"A new inducible expression system in a transformed green alga, Chlorella vulgaris","volume":"10","author":"Niu","year":"2011","journal-title":"Genet. Mol. Res."},{"key":"10.1016\/j.dci.2026.105753_bib22","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.fsi.2019.04.002","article-title":"Insights into teleost interferon-gamma biology: an update","volume":"90","author":"Pereiro","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.dci.2026.105753_bib23","article-title":"In vivo antiviral activitiy of Sanguisorba officinalis roots against viral hemorrhagic septicemia virus in olive flounder Paralichthys olivaceus","volume":"81","author":"Seo","year":"2015","journal-title":"Planta Med."},{"key":"10.1016\/j.dci.2026.105753_bib24","doi-asserted-by":"crossref","first-page":"1522","DOI":"10.3390\/v17111522","article-title":"Development of a mass antiviral screening system using viral hemorrhagic septicemia virus as an RNA surrogate and activity confirmation with a fish rhabdovirus","volume":"17","author":"Shin","year":"2025","journal-title":"Viruses"},{"key":"10.1016\/j.dci.2026.105753_bib25","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1016\/j.fob.2014.09.007","article-title":"Structural and functional characterization of salmon STAT1, STAT2 and IRF9 homologs sheds light on interferon signaling in teleosts","volume":"4","author":"Sobhkhez","year":"2014","journal-title":"FEBS Open Bio"},{"key":"10.1016\/j.dci.2026.105753_bib26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1079\/PAVSNNR20116002","article-title":"Viral haemorrhagic septicaemia (VHS): detection, distribution and combat","volume":"6","author":"Wahli","year":"2011","journal-title":"CABI Rev"},{"key":"10.1016\/j.dci.2026.105753_bib28","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1002\/biot.201500617","article-title":"Chlorella species as hosts for genetic engineering and expression of heterologous proteins: progress, challenge and perspective","volume":"11","author":"Yang","year":"2016","journal-title":"Biotechnol. J."},{"key":"10.1016\/j.dci.2026.105753_bib27","doi-asserted-by":"crossref","first-page":"296","DOI":"10.3390\/v18030296","article-title":"Identification of fish interferon-stimulated genes and their antiviral mechanisms","volume":"18","author":"Yang","year":"2026","journal-title":"Viruses"},{"key":"10.1016\/j.dci.2026.105753_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2023.108936","article-title":"Identification, expression pattern and functional characterization of IFN-\u03b3 involved in activating JAK-STAT pathway in Sebastes schlegeli","volume":"140","author":"Zhang","year":"2023","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.dci.2026.105753_bib30","doi-asserted-by":"crossref","first-page":"2484","DOI":"10.4049\/jimmunol.175.4.2484","article-title":"Identification and bioactivities of IFN-\u03b3 in rainbow trout Oncorhynchus mykiss: the first Th1-type cytokine characterized functionally in fish","volume":"175","author":"Zou","year":"2005","journal-title":"J. Immunol."}],"container-title":["Developmental & Comparative Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0145305X26002090?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0145305X26002090?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T20:55:08Z","timestamp":1793307308000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0145305X26002090"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":30,"alternative-id":["S0145305X26002090"],"URL":"https:\/\/doi.org\/10.1016\/j.dci.2026.105753","relation":{},"ISSN":["0145-305X"],"issn-type":[{"value":"0145-305X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"In vitro inhibitory activity of recombinant olive flounder interferon-\u03b3 produced in Chlorella vulgaris against viral hemorrhagic septicemia virus infection","name":"articletitle","label":"Article Title"},{"value":"Developmental & Comparative Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.dci.2026.105753","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"105753"}}