{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T15:51:06Z","timestamp":1761580266257},"reference-count":27,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2005,2,1]],"date-time":"2005-02-01T00:00:00Z","timestamp":1107216000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2005,2]]},"DOI":"10.1016\/j.diagmicrobio.2004.10.004","type":"journal-article","created":{"date-parts":[[2004,12,24]],"date-time":"2004-12-24T15:47:41Z","timestamp":1103903261000},"page":"85-90","source":"Crossref","is-referenced-by-count":24,"title":["Rapid detection of Neisseria meningitidis in cerebrospinal fluid by one-step polymerase chain reaction of the nspA gene"],"prefix":"10.1016","volume":"51","author":[{"given":"Ivano","family":"de Filippis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos R.S.","family":"do Nascimento","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maysa B.M.","family":"Clementino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea B.","family":"Sereno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Rebelo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadjla N.F.","family":"Souza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee W.","family":"Riley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.diagmicrobio.2004.10.004_bib1","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1002\/1098-2825(2000)14:4<193::AID-JCLA9>3.0.CO;2-2","article-title":"One-step heminested PCR for amplification of Neisseria meningitidis DNA in cerebrospinal fluid","volume":"14","author":"Atobe","year":"2000","journal-title":"J. Clin. Lab. Anal."},{"key":"10.1016\/j.diagmicrobio.2004.10.004_bib2","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1006\/mcpr.1998.0218","article-title":"Evaluation of an extended diagnostic PCR assay for detection and verification of the common causes of bacterial meningitis in CSF and other biological samples","volume":"13","author":"Backman","year":"1998","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.diagmicrobio.2004.10.004_bib3","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1111\/j.1574-6968.1998.tb13001.x","article-title":"False positive diagnosis of meningococcal infection by the IS1106 PCR ELISA","volume":"162","author":"Borrow","year":"1998","journal-title":"FEMS Microbiol. Lett."},{"issue":"9","key":"10.1016\/j.diagmicrobio.2004.10.004_bib4","doi-asserted-by":"crossref","first-page":"4955","DOI":"10.1128\/IAI.67.9.4955-4959.1999","article-title":"Bactericidal and cross-protective activities of a monoclonal antibody directed against Neisseria meningitidis NspA outer membrane protein","volume":"67","author":"Cadieux","year":"1999","journal-title":"Infect. Immun."},{"key":"10.1016\/j.diagmicrobio.2004.10.004_bib5","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1590\/S0074-02761995000100006","article-title":"Development of a collection of bacteria causing meningitis in Rio de Janeiro from 1990 to 1991","volume":"90","author":"de Filippis","year":"1995","journal-title":"Mem. Inst. Oswaldo Cruz"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2004.10.004_bib6","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1080\/09674845.2002.11783649","article-title":"Semi-automation of the polymerase chain reaction for laboratory confirmation of meningococcal disease","volume":"59","author":"Diggle","year":"2002","journal-title":"Br. J. Biomed. Sci."},{"key":"10.1016\/j.diagmicrobio.2004.10.004_bib7","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1099\/jmm.0.05032-0","article-title":"Detection and genotyping of meningococci using a nested PCR approach","volume":"52","author":"Diggle","year":"2003","journal-title":"J. Med. Microbiol."},{"issue":"12","key":"10.1016\/j.diagmicrobio.2004.10.004_bib8","doi-asserted-by":"crossref","first-page":"4518","DOI":"10.1128\/JCM.39.12.4518-4519.2001","article-title":"Automation of fluorescence-based PCR for confirmation of meningococcal disease","volume":"39","author":"Diggle","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.diagmicrobio.2004.10.004_bib9","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1086\/598618","article-title":"Application of polymerase chain reaction to the diagnosis of infectious diseases","volume":"29","author":"Fredricks","year":"1999","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.diagmicrobio.2004.10.004_bib10","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1128\/IAI.60.3.798-803.1992","article-title":"Conserved outer membrane protein of Neisseria meningitidis involved in capsule expression","volume":"60","author":"Frosch","year":"1992","journal-title":"Infect. Immun."},{"key":"10.1016\/j.diagmicrobio.2004.10.004_bib11","unstructured":"Funda\u00e7\u00e3o Nacional de Sa\u00fade\u2013Minist\u00e9rio da Sa\u00fade, Brazil (1999) Boletim Epidemiol\u00f3gico, Ano III [online]. Available at: http:\/\/www.funasa.gov.br."},{"key":"10.1016\/j.diagmicrobio.2004.10.004_bib12","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1111\/j.1574-695X.2000.tb01473.x","article-title":"Evaluation of the Applied Biosystems automated Taqman polymerase chain reaction system for the detection of meningococcal DNA","volume":"28","author":"Guiver","year":"2000","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"10.1016\/j.diagmicrobio.2004.10.004_bib13","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1007\/BF01590946","article-title":"An approach to the identification of the pathogens of bacterial meningitis by the polymerase chain reaction","volume":"14","author":"Hall","year":"1995","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"issue":"4","key":"10.1016\/j.diagmicrobio.2004.10.004_bib14","doi-asserted-by":"crossref","first-page":"2082","DOI":"10.1128\/IAI.68.4.2082-2095.2000","article-title":"Molecular and biological analysis of eight genetic islands that distinguish Neisseria meningitidis from the closely related pathogen Neisseria gonorrhoeae","volume":"68","author":"Klee","year":"2000","journal-title":"Infect. Immun."},{"issue":"8","key":"10.1016\/j.diagmicrobio.2004.10.004_bib15","doi-asserted-by":"crossref","first-page":"2205","DOI":"10.1128\/JCM.36.8.2205-2209.1998","article-title":"Diagnosis of meningococcal meningitis by broad-range bacterial PCR with cerebrospinal fluid","volume":"36","author":"Kotilainen","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.diagmicrobio.2004.10.004_bib16","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.1016\/0140-6736(91)93262-8","article-title":"Rapid diagnosis of meningococcal meningitis by polymerase chain reaction","volume":"337","author":"Kristiansen","year":"1991","journal-title":"Lancet"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2004.10.004_bib17","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1007\/s100960000290","article-title":"Novel genus-specific PCR-based assays for rapid identification of Neisseria species and Neisseria meningitidis","volume":"19","author":"Lansac","year":"2000","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"issue":"6","key":"10.1016\/j.diagmicrobio.2004.10.004_bib18","doi-asserted-by":"crossref","first-page":"2076","DOI":"10.1128\/JCM.38.6.2076-2080.2000","article-title":"Use of PCR with universal primers and restriction endonuclease digestions for detection and identification of common bacterial pathogens in cerebrospinal fluid","volume":"38","author":"Lu","year":"2000","journal-title":"J. Clin. Microbiol."},{"issue":"7","key":"10.1016\/j.diagmicrobio.2004.10.004_bib19","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1084\/jem.185.7.1173","article-title":"Highly conserved Neisseria meningitidis surface protein confers protection against experimental infection","volume":"185","author":"Martin","year":"1997","journal-title":"J. Exp. Med."},{"issue":"11","key":"10.1016\/j.diagmicrobio.2004.10.004_bib20","doi-asserted-by":"crossref","first-page":"5664","DOI":"10.1128\/IAI.67.11.5664-5675.1999","article-title":"Differences in surface expression of NspA among Neisseria meningitidis group B strains","volume":"67","author":"Moe","year":"1999","journal-title":"Infect. Immun."},{"issue":"7","key":"10.1016\/j.diagmicrobio.2004.10.004_bib21","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1128\/JCM.34.7.1637-1640.1996","article-title":"PCR of peripheral blood for diagnosis of meningococcal disease","volume":"34","author":"Newcombe","year":"1996","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.diagmicrobio.2004.10.004_bib22","doi-asserted-by":"crossref","first-page":"1432","DOI":"10.1016\/0140-6736(92)92622-M","article-title":"Polymerase chain reaction for diagnosis of meningococcal meningitis","volume":"340","author":"Ni","year":"1992","journal-title":"Lancet"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2004.10.004_bib23","doi-asserted-by":"crossref","first-page":"2855","DOI":"10.1128\/IAI.67.6.2855-2861.1999","article-title":"Antigenic and molecular conservation of the gonococcal NspA protein","volume":"67","author":"Plante","year":"1999","journal-title":"Infect. Immun."},{"key":"10.1016\/j.diagmicrobio.2004.10.004_bib24","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1080\/003130200104565","article-title":"Detection and serogroup determination of Neisseria meningitidis in CSF by polymerase chain reaction (PCR)","volume":"32","author":"Porrit","year":"2000","journal-title":"Pathology"},{"key":"10.1016\/j.diagmicrobio.2004.10.004_bib25","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/S1286-4579(00)00356-7","article-title":"Epidemiology and pathogenesis of Neisseria meningitidis","volume":"2","author":"Tzeng","year":"2000","journal-title":"Microbes Infect."},{"issue":"27","key":"10.1016\/j.diagmicrobio.2004.10.004_bib26","doi-asserted-by":"crossref","first-page":"24825","DOI":"10.1074\/jbc.M302803200","article-title":"Crystal structure of neisserial surface protein A (NspA), a conserved outer membrane protein with vaccine potential","volume":"278","author":"Vandeputte-Rutten","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.diagmicrobio.2004.10.004_bib27","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1046\/j.1365-2672.2003.01839.x","article-title":"Employment of broad-range 16S rRNA PCR to detect aetiological agents of infection from clinical specimens in patients with acute meningitis\u2014rapid separation of 16S rRNA PCR amplicons without the need for cloning","volume":"94","author":"Xu","year":"2003","journal-title":"J. Appl. Microbiol."}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889304002147?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889304002147?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,4,4]],"date-time":"2020-04-04T23:09:57Z","timestamp":1586041797000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889304002147"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,2]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2005,2]]}},"alternative-id":["S0732889304002147"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2004.10.004","relation":{},"ISSN":["0732-8893"],"issn-type":[{"type":"print","value":"0732-8893"}],"subject":[],"published":{"date-parts":[[2005,2]]}}}