{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T10:48:50Z","timestamp":1776854930232,"version":"3.51.2"},"reference-count":31,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2013,1]]},"DOI":"10.1016\/j.diagmicrobio.2012.09.014","type":"journal-article","created":{"date-parts":[[2012,10,25]],"date-time":"2012-10-25T00:30:18Z","timestamp":1351125018000},"page":"37-41","source":"Crossref","is-referenced-by-count":15,"title":["Direct mecA polymerase chain reaction testing of blood culture bottles growing Gram-positive cocci and the clinical potential in optimizing antibiotic therapy for staphylococcal bacteremia"],"prefix":"10.1016","volume":"75","author":[{"given":"Bing","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Jessamine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Desjardins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baldwin","family":"Toye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karam","family":"Ramotar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0005","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1128\/JCM.37.5.1415-1418.1999","article-title":"Clinical and financial benefits of rapid bacterial identification and antimicrobial susceptibility testing","volume":"37","author":"Barenfanger","year":"1999","journal-title":"J Clin Microbiol"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0010","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/BF03256265","article-title":"Rapid diagnostics for methicillin-resistant Staphylococcus aureus: current status","volume":"12","author":"Carroll","year":"2008","journal-title":"Mol Diagn Ther"},{"key":"10.1016\/j.diagmicrobio.2012.09.014_bb0020","unstructured":"Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial disk susceptibility test (M02-A10). Wayne, PA: CLSI; 2008."},{"key":"10.1016\/j.diagmicrobio.2012.09.014_bb0015","unstructured":"Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing; 19th informational supplement (M100-S19). Wayne, PA: CLSI; 2006."},{"issue":"Suppl 5","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0155","doi-asserted-by":"crossref","first-page":"S386","DOI":"10.1086\/533595","article-title":"Management of methicillin-resistant Staphylococcus bacteremia","volume":"46","author":"Cosgrove","year":"2008","journal-title":"Clin Infect Dis"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0025","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1086\/345476","article-title":"Comparison of mortality associated with methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteremia: a meta-analysis","volume":"36","author":"Cosgrove","year":"2003","journal-title":"Clin Infect Dis"},{"issue":"12","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0030","doi-asserted-by":"crossref","first-page":"1543","DOI":"10.1086\/518452","article-title":"Counterpoint: vancomycin and Staphylococcus aureus\u2014an antibiotic enters obsolescence","volume":"44","author":"Deresinski","year":"2007","journal-title":"Clin Infect Dis"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0035","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1128\/JCM.06169-11","article-title":"Clinical impact of a real-time PCR assay for rapid identification of staphylococcal bacteremia","volume":"50","author":"Frye","year":"2011","journal-title":"J Clin Microbiol"},{"issue":"8","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0040","doi-asserted-by":"crossref","first-page":"2961","DOI":"10.1128\/JCM.39.8.2961-2963.2001","article-title":"Correlation between genotype and phenotypic categorization of staphylococci based on methicillin susceptibility and resistance","volume":"39","author":"Gradelski","year":"2001","journal-title":"J Clin Microbiol"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0045","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1007\/s10096-007-0368-3","article-title":"Rapid detection of methicillin-resistant staphylococci by real-time PCR directly from positive blood culture bottles","volume":"26","author":"Grobner","year":"2007","journal-title":"Eur J Clin Microbiol Infect Dis"},{"issue":"8","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0050","doi-asserted-by":"crossref","first-page":"3942","DOI":"10.1128\/JCM.41.8.3942-3944.2003","article-title":"Clinical impact of a PCR assay for identification of Staphylococcus aureus and determination of methicillin resistance directly from blood cultures","volume":"41","author":"Hallin","year":"2003","journal-title":"J Clin Microbiol"},{"issue":"11","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0055","doi-asserted-by":"crossref","first-page":"5041","DOI":"10.1128\/JCM.42.11.5041-5046.2004","article-title":"Evaluation of the BD PHOENIX automated microbiology system for detection of methicillin resistance in coagulase-negative staphylococci","volume":"42","author":"Horstkotte","year":"2004","journal-title":"J Clin Microbiol"},{"issue":"9","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0060","doi-asserted-by":"crossref","first-page":"3291","DOI":"10.1128\/JCM.40.9.3291-3295.2002","article-title":"Rapid detection of methicillin resistance in coagulase-negative staphylococci with the VITEK 2 system","volume":"40","author":"Horstkotte","year":"2002","journal-title":"J Clin Microbiol"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0065","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1128\/JCM.38.2.752-754.2000","article-title":"Correlation of oxacillin MIC with mecA gene carriage in coagulase-negative staphylococci","volume":"38","author":"Hussain","year":"2000","journal-title":"J Clin Microbiol"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0070","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1378\/chest.118.1.146","article-title":"The influence of inadequate antimicrobial treatment of bloodstream infections on patient outcomes in the ICU setting","volume":"118","author":"Ibrahim","year":"2000","journal-title":"Chest"},{"key":"10.1016\/j.diagmicrobio.2012.09.014_bb0075","first-page":"1.20.11","volume":"Vol. 1","year":"1992"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0145","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1086\/600295","article-title":"Salvage treatment for persistent methicillin-resistant Staphylococcus aureus bacteremia: efficacy of linezolid with or without carbapenem","volume":"49","author":"Jang","year":"2009","journal-title":"Clin Infec Dis"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0080","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/jac\/dkn527","article-title":"Comparison of three phenotypic techniques for detection of methicillin resistance in Staphylococcus spp. reveals a species-dependent performance","volume":"63","author":"John","year":"2009","journal-title":"J Antimicrob Chemother"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0085","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1128\/AAC.00700-07","article-title":"Outcome of vancomycin treatment in patients with methicillin-susceptible Staphylococcus aureus bacteremia","volume":"52","author":"Kim","year":"2008","journal-title":"Antimicrob Agents Chemother"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0090","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1378\/chest.115.2.462","article-title":"Inadequate antimicrobial treatment of infections: a risk factor for hospital mortality among critically ill patients","volume":"115","author":"Kollef","year":"1999","journal-title":"Chest"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0095","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1007\/BF02184683","article-title":"Detection of borderline oxacillin-resistant Staphylococcus aureus and differentiation from methicillin-resistant strains","volume":"9","author":"Liu","year":"1990","journal-title":"Eur J Clin Microbiol Infect Dis"},{"issue":"Suppl 3","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0100","doi-asserted-by":"crossref","first-page":"S184","DOI":"10.1086\/519471","article-title":"Alternatives to vancomycin for the treatment of methicillin-resistant Staphylococcus aureus infections","volume":"45","author":"Micek","year":"2007","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2012.09.014_bb0105","first-page":"390","volume":"Vol. 1","author":"Murray","year":"2007"},{"issue":"20","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0150","doi-asserted-by":"crossref","first-page":"1598","DOI":"10.1001\/jama.1994.03510440058033","article-title":"Nosocomial bloodstream infection in critically ill patients. Excess length of stay, extra costs, and attributable mortality","volume":"271","author":"Pittet","year":"1994","journal-title":"JAMA"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0110","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.ajic.2009.12.004","article-title":"International Nosocomial Infection Control Consortium (INICC) report, data summary for 2003\u20132008, issued June 2009","volume":"38","author":"Rosenthal","year":"2010","journal-title":"Am J Infect Control"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0115","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1128\/JCM.02250-07","article-title":"Accuracy and potential usefulness of triplex real-time PCR for improving antibiotic treatment of patients with blood cultures showing clustered Gram-positive cocci on direct smears","volume":"46","author":"Ruimy","year":"2008","journal-title":"J Clin Microbiol"},{"issue":"8","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0120","doi-asserted-by":"crossref","first-page":"2069","DOI":"10.1097\/01.CCM.0000227655.41566.3E","article-title":"Methicillin-resistant Staphylococcus aureus sterile-site infection: the importance of appropriate initial antimicrobial treatment","volume":"34","author":"Schramm","year":"2006","journal-title":"Crit Care Med"},{"issue":"7","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0125","doi-asserted-by":"crossref","first-page":"2659","DOI":"10.1128\/JCM.40.7.2659-2661.2002","article-title":"Rapid identification of Staphylococcus aureus and the mecA gene from BacT\/ALERT blood culture bottles by using the LightCycler system","volume":"40","author":"Shrestha","year":"2002","journal-title":"J Clin Microbiol"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0130","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1086\/651313","article-title":"Methicillin-resistant Staphylococcus aureus colonization or infection in Canada: National Surveillance and Changing Epidemiology, 1995\u20132007","volume":"31","author":"Simor","year":"2010","journal-title":"Infect Control Hosp Epidemiol"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0135","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1086\/510386","article-title":"Use of vancomycin or first-generation cephalosporins for the treatment of hemodialysis-dependent patients with methicillin-susceptible Staphylococcus aureus bacteremia","volume":"44","author":"Stryjewski","year":"2007","journal-title":"Clin Infect Dis"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2012.09.014_bb0140","doi-asserted-by":"crossref","first-page":"3785","DOI":"10.1128\/JCM.39.10.3785-3788.2001","article-title":"Performance of eight methods, including two new rapid methods, for detection of oxacillin resistance in a challenge set of Staphylococcus aureus organisms","volume":"39","author":"Swenson","year":"2001","journal-title":"J Clin Microbiol"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889312003926?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889312003926?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,7,15]],"date-time":"2020-07-15T03:28:32Z","timestamp":1594783712000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889312003926"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["S0732889312003926"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2012.09.014","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2013,1]]}}}