{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T04:13:01Z","timestamp":1772165581301,"version":"3.50.1"},"reference-count":12,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2013,9]]},"DOI":"10.1016\/j.diagmicrobio.2013.05.010","type":"journal-article","created":{"date-parts":[[2013,6,19]],"date-time":"2013-06-19T21:47:47Z","timestamp":1371678467000},"page":"87-89","source":"Crossref","is-referenced-by-count":6,"title":["Cloning, expression, and purification of the hemolysin\/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development"],"prefix":"10.1016","volume":"77","author":[{"given":"Eugene Boon Beng","family":"Ong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy Amilda","family":"Anthony","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aziah","family":"Ismail","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asma","family":"Ismail","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theam Soon","family":"Lim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.diagmicrobio.2013.05.010_bb0005","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0001-706X(98)00095-3","article-title":"Rapid and reliable serological diagnosis of enteric fever: comparative sensitivity and specificity of Typhidot and Typhidot-M tests in febrile Malaysian children","volume":"72","author":"Choo","year":"1999","journal-title":"Acta Trop"},{"key":"10.1016\/j.diagmicrobio.2013.05.010_bb0010","doi-asserted-by":"crossref","DOI":"10.1177\/1010539512458521","article-title":"Identification of carriers among individuals recruited in the typhoid Registry in Malaysia using stool culture, polymerase chain reaction, and dot enzyme immunoassay as detection tools","author":"Chua","year":"2012","journal-title":"Asia Pac J Public Health"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2013.05.010_bb0015","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.mib.2006.02.003","article-title":"Regulation of virulence by members of the MarR\/SlyA family","volume":"9","author":"Ellison","year":"2006","journal-title":"Curr Opin Microbiol"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2013.05.010_bb0020","doi-asserted-by":"crossref","first-page":"169","DOI":"10.3855\/jidc.1339","article-title":"Evaluation of a newly developed ELISA against Widal, TUBEX-TF and Typhidot for typhoid fever surveillance","volume":"5","author":"Fadeel","year":"2011","journal-title":"J Infect Dev Ctries"},{"key":"10.1016\/j.diagmicrobio.2013.05.010_bb0025","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1038\/srep01043","article-title":"Immune profiling with a Salmonella Typhi antigen microarray identifies new diagnostic biomarkers of human typhoid","volume":"3","author":"Liang","year":"2013","journal-title":"Sci Rep"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2013.05.010_bb0030","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.diagmicrobio.2011.03.020","article-title":"Validation of the Dri-Dot Latex agglutination and IgM lateral flow assays for the diagnosis of typhoid fever in an Egyptian population","volume":"70","author":"Nakhla","year":"2011","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2013.05.010_bb0035","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1099\/00222615-29-3-195","article-title":"A simple adherence test for detection of IgM antibodies in typhoid","volume":"29","author":"Ong","year":"1989","journal-title":"J Med Microbiol"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2013.05.010_bb0040","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1046\/j.1365-2958.1999.01435.x","article-title":"Molecular analysis of the cytolytic protein ClyA (SheA) from Escherichia coli","volume":"32","author":"Oscarsson","year":"1999","journal-title":"Mol Microbiol"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2013.05.010_bb0045","doi-asserted-by":"crossref","first-page":"5759","DOI":"10.1128\/IAI.70.10.5759-5769.2002","article-title":"Characterization of a pore-forming cytotoxin expressed by Salmonella enterica serovars typhi and paratyphi A","volume":"70","author":"Oscarsson","year":"2002","journal-title":"Infect Immun"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2013.05.010_bb0050","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.diagmicrobio.2007.12.014","article-title":"Simple, rapid, and affordable point-of-care test for the serodiagnosis of typhoid fever","volume":"61","author":"Pastoor","year":"2008","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2013.05.010_bb0055","series-title":"The condensed protocols from molecular cloning: a laboratory manual","author":"Sambrook","year":"2006"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2013.05.010_bb0060","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.ijmm.2008.06.004","article-title":"ClyA cytolysin from Salmonella: distribution within the genus, regulation of expression by SlyA, and pore-forming characteristics","volume":"299","author":"von Rhein","year":"2009","journal-title":"Int J Med Microbiol"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889313002782?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889313002782?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T01:21:48Z","timestamp":1539566508000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889313002782"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["S0732889313002782"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2013.05.010","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2013,9]]}}}