{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T21:35:28Z","timestamp":1780608928478,"version":"3.54.1"},"reference-count":29,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2014,1]]},"DOI":"10.1016\/j.diagmicrobio.2013.09.010","type":"journal-article","created":{"date-parts":[[2013,9,28]],"date-time":"2013-09-28T17:46:30Z","timestamp":1380390390000},"page":"16-23","source":"Crossref","is-referenced-by-count":19,"title":["Coupling electrochemical response of a DNA biosensor with PCR for Neisseria gonorrhoeae detection"],"prefix":"10.1016","volume":"78","author":[{"given":"Rachna","family":"Verma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seema","family":"Sood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renu","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gajjala","family":"Sumana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manju","family":"Bala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinod K.","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyotish C.","family":"Samantaray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravindra M.","family":"Pandey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bansi D.","family":"Malhotra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0060","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1111\/j.1651-2227.2006.00178.x","article-title":"Understanding diagnostic tests 3: receiver operating characteristic curves","volume":"96","author":"Akobeng","year":"2007","journal-title":"Acta Paediatr"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0090","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1017\/S0950268809990148","article-title":"Experience with an external quality assurance scheme for antimicrobial susceptibility testing of Neisseria gonorrhoeae in India, 2001\u20132007","volume":"138","author":"Bala","year":"2010","journal-title":"Epidemiol Infect"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0015","doi-asserted-by":"crossref","first-page":"50","DOI":"10.3390\/jpm2020050","article-title":"Infectious disease management through point-of-care personalized medicine molecular diagnostic technologies","volume":"2","author":"Bissonnette","year":"2012","journal-title":"J Pers Med"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0080","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1177\/104063871102300102","article-title":"Selection of a cutoff value for real-time polymerase chain reaction results to fit a diagnostic purpose: analytical and epidemiologic approaches","volume":"23","author":"Caraguel","year":"2011","journal-title":"J Vet Diagn Invest"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0020","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1038\/nbt873","article-title":"Electrochemical DNA sensors","volume":"21","author":"Drummond","year":"2003","journal-title":"Nat Biotechnol"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0110","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1002\/1521-4109(200103)13:3<219::AID-ELAN219>3.0.CO;2-7","article-title":"Methylene blue as a novel electrochemical hybridization indicator","volume":"13","author":"Erdem","year":"2001","journal-title":"Electroanalysis"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0040","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/1475-2859-3-2","article-title":"Identification of pathogenic microbial cells and spores by electrochemical detection on a biochip","volume":"3","author":"Gabig-Ciminska","year":"2004","journal-title":"Microb Cell Fact"},{"issue":"11","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0095","doi-asserted-by":"crossref","first-page":"5653","DOI":"10.1128\/JCM.43.11.5653-5659.2005","article-title":"Specific and sensitive detection of Neisseria gonorrhoeae in clinical specimens by real-time PCR","volume":"43","author":"Geraats-Peters","year":"2005","journal-title":"J Clin Microbiol"},{"issue":"13","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0125","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1002\/1521-4109(200207)14:13<949::AID-ELAN949>3.0.CO;2-O","article-title":"DNA sensor for recognition of native yeast DNA sequence with methylene blue as an electrochemical hybridization indicator","volume":"14","author":"Gu","year":"2002","journal-title":"Electroanalysis"},{"issue":"11","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0010","doi-asserted-by":"crossref","first-page":"e14144","DOI":"10.1371\/journal.pone.0014144","article-title":"Perceptions of an ideal point-of-care test for sexually transmitted infections \u2013 a qualitative study of focus group discussions with medical providers","volume":"5","author":"Hsieh","year":"2010","journal-title":"PLoS One"},{"issue":"5","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0140","first-page":"523","article-title":"Electrochemical detection of specific DNA sequences from PCR amplicons on carbon and mercury electrodes using Meldola's blue as an intercalator","volume":"28","author":"Kerman","year":"2004","journal-title":"Turk J Chem"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0145","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.elecom.2004.01.012","article-title":"Electrochemical DNA quantification based on aggregation induced by Hoechst 33258","volume":"6","author":"Kobayashi","year":"2004","journal-title":"Electrochem Commun"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0045","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1128\/JCM.44.2.561-570.2006","article-title":"Use of electrochemical DNA biosensors for rapid molecular identification of uropathogens in clinical urine specimens","volume":"44","author":"Liao","year":"2006","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2013.09.010_bb0075","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1002\/elan.200804227","article-title":"Designs of Enterobacteriaceae Lac Z gene DNA gold screen printed biosensors","volume":"20","author":"Loaiza","year":"2008","journal-title":"Electroanalysis"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0030","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1039\/B815307H","article-title":"Ultrasensitive detection of coliforms by means of direct asymmetric PCR combined with disposable magnetic amperometric genosensors","volume":"134","author":"Loaiza","year":"2009","journal-title":"Analyst"},{"issue":"5","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0050","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/S0039-9140(01)00650-6","article-title":"Electrochemical DNA biosensor for the detection of TT and Hepatitis B virus from PCR amplified real samples by using methylene blue","volume":"56","author":"Meric","year":"2002","journal-title":"Talanta"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0085","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1007\/s10654-011-9561-x","article-title":"Sensitivity \u00d7 PPV is a recognized test called the clinical utility index (CUI+)","volume":"26","author":"Mitchell","year":"2011","journal-title":"Eur J Epidemiol"},{"key":"10.1016\/j.diagmicrobio.2013.09.010_bb0025","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1039\/c0ay00755b","article-title":"Electrochemical genosensors based on PCR strategies for microorganisms detection and quantification","volume":"3","author":"Pedrero","year":"2011","journal-title":"Anal Methods"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0055","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.jbiotec.2010.09.935","article-title":"DNA biosensor for detection of Neisseria gonorrhoeae causing sexually transmitted disease","volume":"150","author":"Singh","year":"2010","journal-title":"J Biotechnol"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0105","first-page":"358","article-title":"Guidelines for the use and interpretation of nucleic acid detection tests for Neisseria gonorrhoeae in Australia: a position paper on behalf of the Public Health Laboratory Network","volume":"29","author":"Smith","year":"2005","journal-title":"Commun Dis Intell Q Rep"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0115","doi-asserted-by":"crossref","first-page":"2118","DOI":"10.3390\/s8042118","article-title":"Application of DNA hybridization biosensor as a screening method for the detection of genetically modified food components","volume":"8","author":"Tichoniuk","year":"2008","journal-title":"Sensors"},{"key":"10.1016\/j.diagmicrobio.2013.09.010_bb0005","unstructured":"UNAIDS. The public health approach to STD control: UNAIDS technical update. Geneva: UNAIDS 1998."},{"issue":"11","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0065","doi-asserted-by":"crossref","first-page":"2110","DOI":"10.1017\/S0950268811002883","article-title":"Evaluation of an opa gene-based nucleic acid amplification test for detection of Neisseria gonorrhoeae in urogenital samples in North India","volume":"140","author":"Verma","year":"2012","journal-title":"Epidemiol Infect"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0130","doi-asserted-by":"crossref","first-page":"14","DOI":"10.2214\/ajr.184.1.01840014","article-title":"Clinical evaluation of diagnostic tests","volume":"184","author":"Weinstein","year":"2005","journal-title":"AJR Am J Roentgenol"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0100","doi-asserted-by":"crossref","first-page":"3","DOI":"10.2353\/jmoldx.2006.050045","article-title":"Nucleic acid amplification testing for Neisseria gonorrhoeae: an ongoing challenge","volume":"8","author":"Whiley","year":"2006","journal-title":"J Mol Diagn"},{"key":"10.1016\/j.diagmicrobio.2013.09.010_bb0070","series-title":"Laboratory Diagnosis of Gonorrhoea. WHO Regional Publication, SouthEast Asia series no. 33","author":"World Health Organization (WHO)","year":"1999"},{"issue":"18","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0120","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1002\/1521-4109(200210)14:18<1299::AID-ELAN1299>3.0.CO;2-Y","article-title":"Evidence for the direct interaction between methylene blue and guanine bases using DNA-modified carbon paste electrodes","volume":"14","author":"Yang","year":"2002","journal-title":"Electroanalysis"},{"issue":"9","key":"10.1016\/j.diagmicrobio.2013.09.010_bb0035","doi-asserted-by":"crossref","first-page":"5649","DOI":"10.3390\/s8095649","article-title":"Electrochemical behaviors of methylene blue on DNA modified electrode and its application to the detection of PCR product from NOS sequence","volume":"8","author":"Zhu","year":"2008","journal-title":"Sensors"},{"key":"10.1016\/j.diagmicrobio.2013.09.010_bb0135","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1161\/CIRCULATIONAHA.105.594929","article-title":"Receiver-operating characteristic analysis for evaluating diagnostic tests and predictive models","volume":"115","author":"Zou","year":"2007","journal-title":"Circulation"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889313005312?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889313005312?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,10,12]],"date-time":"2018-10-12T10:59:49Z","timestamp":1539341989000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889313005312"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["S0732889313005312"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2013.09.010","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2014,1]]}}}