{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:54:00Z","timestamp":1790970840065,"version":"4.1.0"},"reference-count":28,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2014,4]]},"DOI":"10.1016\/j.diagmicrobio.2013.10.015","type":"journal-article","created":{"date-parts":[[2013,10,31]],"date-time":"2013-10-31T18:10:14Z","timestamp":1383243014000},"page":"418-421","source":"Crossref","is-referenced-by-count":25,"title":["Detection of Leishmania infantum in 4 different dog samples by real-time PCR and ITS-1 nested PCR"],"prefix":"10.1016","volume":"78","author":[{"given":"Aline Leandra","family":"Carvalho Ferreira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virg\u00ednia Mendes","family":"Carregal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sidney","family":"de Almeida Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Souza","family":"Leite","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antero Silva Ribeiro","family":"de Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.diagmicrobio.2013.10.015_bb0145","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.diagmicrobio.2013.03.017","article-title":"Canine visceral leishmaniasis: diagnostic approaches based on polymerase chain reaction employing different biological samples","volume":"76","author":"Almeida","year":"2013","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0010","doi-asserted-by":"crossref","first-page":"251","DOI":"10.4269\/ajtmh.1995.53.251","article-title":"Comparison of the polymerase chain reaction and serology for the detection of canine visceral leishmaniasis","volume":"53","author":"Ashford","year":"1995","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0015","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1099\/00221287-120-1-21","article-title":"The Ultrastructure of Kinetoplast DNA with particular reference to the interpretation of dark field electron microscopy images of isolated, purified networks","volume":"11","author":"Barker","year":"1980","journal-title":"Micron"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0020","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1128\/JCM.02258-07","article-title":"Quantitative real-time PCR is not more sensitive than \u201cconventional\u201d PCR","volume":"46","author":"Bastien","year":"2008","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0025","doi-asserted-by":"crossref","first-page":"e1291","DOI":"10.1371\/journal.pntd.0001291","article-title":"Prevalence and factors associated with Leishmania infantum infection of dogs from an urban area of Brazil as identified by molecular methods","volume":"5","author":"Coura-Vital","year":"2011","journal-title":"PLoS Negl Trop Dis"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0085","series-title":"Nota t\u00e9cnica conjunta no 01\/2001 \u2013 CGDT\/DEVIT\/SUS\/MS. Esclarecimento sobre a substitui\u00e7\u00e3o do protocolo diagn\u00f3stico da leishmaniose visceral canina (LVC)","author":"da Sa\u00fade","year":"2011"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0030","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.tvjl.2008.05.018","article-title":"The development of a real-time PCR assay for the quantification of Leishmania infantum DNA in canine blood","volume":"182","author":"De Paiva Cavalcanti","year":"2009","journal-title":"Vet J"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0035","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.vetpar.2007.12.022","article-title":"Evaluation of the conjunctival swab for canine visceral leishmaniasis diagnosis by PCR-hybridization in Minas Gerais State, Brazil","volume":"152","author":"Ferreira","year":"2008","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0040","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.vetpar.2006.01.011","article-title":"Advantages of real-time PCR assay for diagnosis and monitoring of canine leishmaniosis","volume":"137","author":"Francino","year":"2006","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0045","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1016\/j.ijpara.2005.07.001","article-title":"The current status of zoonotic leishmaniasis and approaches to disease control","volume":"35","author":"Gramiccia","year":"2005","journal-title":"Int J Parasitol"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0050","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1128\/JCM.40.1.210-215.2002","article-title":"Comparison of six PCR methods using peripheral blood for detection of canine visceral leishmaniasis","volume":"40","author":"Lachaud","year":"2002","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0055","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.vetpar.2010.02.020","article-title":"PCR diagnosis of visceral leishmaniasis in asymptomatic dogs using conjunctival swab samples","volume":"170","author":"Leite","year":"2010","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0060","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1590\/S1984-29612011000100008","article-title":"The use of conjunctival swab samples for PCR screening for visceral leishmaniasis in vaccinated dogs","volume":"20","author":"Leite","year":"2011","journal-title":"Rev Bras Parasitol Vet"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0065","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.vetpar.2008.07.028","article-title":"Methods for diagnosis of canine leishmaniasis and immune response to infection","volume":"158","author":"Maia","year":"2008","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0070","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.tvjl.2007.08.009","article-title":"Diagnosis of canine leishmaniasis: conventional and molecular techniques using different tissues","volume":"179","author":"Maia","year":"2009","journal-title":"Vet J"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0075","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/1756-3305-4-57","article-title":"Canine leishmaniasis: the key points for qPCR result interpretation","volume":"4","author":"Mart\u00ednez","year":"2011","journal-title":"Parasitol Vector"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0080","doi-asserted-by":"crossref","first-page":"5249","DOI":"10.1128\/JCM.42.11.5249-5255.2004","article-title":"Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity","volume":"42","author":"Mary","year":"2004","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0090","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.vetpar.2012.10.013","article-title":"Canine visceral leishmaniasis: a comparative study of real-time PCR, conventional PCR and direct agglutination on sera for the detection of Leishmania infantum infection","volume":"192","author":"Mohammadiha","year":"2013","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0095","first-page":"5","article-title":"Polymerase chain reaction evaluation for canine visceral leishmaniasis diagnosis in dog blood samples","volume":"16","author":"Nunes","year":"2007","journal-title":"Rev Bras Parasitol"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0100","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.rvsc.2009.02.005","article-title":"Comparison of PCR methods for diagnosis of canine visceral leishmaniasis in conjunctival swab samples","volume":"87","author":"Pilatti","year":"2009","journal-title":"Res Vet Sci"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0150","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1128\/CVI.00420-06","article-title":"Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs","volume":"14","author":"Porrozzi","year":"2007","journal-title":"Clin Vaccine Immunol"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0110","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/S0732-8893(03)00093-2","article-title":"PCR diagnosis and characterization of Leishmania in local and imported clinical samples","volume":"47","author":"Sch\u00f5nian","year":"2003","journal-title":"Diagn Microbiol Infec Dis"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0115","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1128\/JCM.41.4.1529-1535.2003","article-title":"Detection, differentiation and quantitation of pathogenic Leishmania organisms by fluorescence resonance energy transfer-based real-time PCR assay","volume":"41","author":"Schulz","year":"2003","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0120","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetpar.2009.05.022","article-title":"Directions for the diagnosis, clinical staging, treatment and prevention of canine leishmaniosis","volume":"165","author":"Solano-Gallego","year":"2009","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0155","doi-asserted-by":"crossref","first-page":"1729","DOI":"10.1086\/383281","article-title":"Polymerase chain reaction using noninvasively obtained samples, for the detection of Leishmania infantum DNA in dogs","volume":"189","author":"Strauss-Ayali","year":"2004","journal-title":"J Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0160","doi-asserted-by":"crossref","first-page":"287","DOI":"10.4269\/ajtmh.1995.52.287","article-title":"Control of zoonotic visceral leishmaniasis: is it time to change strategies?","volume":"52","author":"Tesh","year":"1995","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0135","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.vetpar.2009.06.027","article-title":"Leishmania spp. and\/or Trypanosoma cruzi diagnosis in dogs from endemic and nonendemic areas for canine visceral leishmaniasis","volume":"164","author":"Troncarelli","year":"2009","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.diagmicrobio.2013.10.015_bb0140","doi-asserted-by":"crossref","first-page":"5560","DOI":"10.1128\/JCM.43.11.5560-5566.2005","article-title":"Quantitative nucleic acid sequence-based assay as a new molecular tool for detection and quantification of Leishmania parasites in skin biopsy samples","volume":"43","author":"Van der Meide","year":"2005","journal-title":"J Clin Microbiol"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S073288931300552X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S073288931300552X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,7,31]],"date-time":"2019-07-31T17:17:21Z","timestamp":1564593441000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S073288931300552X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["S073288931300552X"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2013.10.015","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2014,4]]}}}