{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T01:34:24Z","timestamp":1784252064313,"version":"3.55.0"},"reference-count":16,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2014,4]]},"DOI":"10.1016\/j.diagmicrobio.2013.12.009","type":"journal-article","created":{"date-parts":[[2013,12,21]],"date-time":"2013-12-21T11:45:48Z","timestamp":1387626348000},"page":"343-346","source":"Crossref","is-referenced-by-count":15,"title":["Evaluation of nested polymerase chain reaction for the early detection of Leptospira spp. DNA in serum samples from patients with leptospirosis"],"prefix":"10.1016","volume":"78","author":[{"given":"Roberta Morozetti","family":"Blanco","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliete Cal\u00f3","family":"Romero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"9","key":"10.1016\/j.diagmicrobio.2013.12.009_bb0005","doi-asserted-by":"crossref","first-page":"e7093","DOI":"10.1371\/journal.pone.0007093","article-title":"Development and validation of a real-time PCR for detection of pathogenic Leptospira species in clinical materials","volume":"4","author":"Ahmed","year":"2009","journal-title":"PLoS One"},{"key":"10.1016\/j.diagmicrobio.2013.12.009_bb0010","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1128\/JCM.41.2.803-809.2003","article-title":"Evaluation of four commercially available rapid serologic tests for diagnosis of leptospirosis","volume":"41","author":"Bajani","year":"2003","journal-title":"J Clin Microbiol"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2013.12.009_bb0015","doi-asserted-by":"crossref","first-page":"167","DOI":"10.33073\/pjm-2010-026","article-title":"Simultaneous detection and differentiation of pathogenic and nonpathogenic Leptospira spp. by multiplex real-time PCR (TaqMan) assay","volume":"59","author":"Bedir","year":"2010","journal-title":"Pol J Microbiol"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2013.12.009_bb0020","doi-asserted-by":"crossref","first-page":"2154","DOI":"10.1128\/JCM.02452-10","article-title":"Comparison of real-time PCR assays for detection of pathogenic Leptospira spp. in blood and identification of variations in target sequences","volume":"49","author":"Bourhy","year":"2011","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2013.12.009_bb0025","series-title":"Leptospira and leptospirosis","author":"Faine","year":"1999"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2013.12.009_bb0030","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.rvsc.2007.09.005","article-title":"The development of a real-time PCR to detect pathogenic Leptospira species in kidney tissue","volume":"85","author":"Fearnley","year":"2008","journal-title":"Res Vet Sci"},{"issue":"9181","key":"10.1016\/j.diagmicrobio.2013.12.009_bb0035","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1016\/S0140-6736(99)80012-9","article-title":"Urban epidemic of severe leptospirosis in Brazil. Salvador Leptospirosis Study Group","volume":"354","author":"Ko","year":"1999","journal-title":"Lancet"},{"key":"10.1016\/j.diagmicrobio.2013.12.009_bb0040","doi-asserted-by":"crossref","first-page":"745","DOI":"10.4269\/ajtmh.2002.66.745","article-title":"Evaluation of two enzyme-linked immunosorbent assay methods for detection of immunoglobulin M antibodies in acute leptospirosis","volume":"66","author":"Levett","year":"2002","journal-title":"Am J Trop Med Hyg"},{"issue":"9","key":"10.1016\/j.diagmicrobio.2013.12.009_bb0045","doi-asserted-by":"crossref","first-page":"2219","DOI":"10.1128\/JCM.30.9.2219-2224.1992","article-title":"Polymerase chain reaction for detection of Leptospira spp. in clinical samples","volume":"30","author":"M\u00e9rien","year":"1992","journal-title":"J Clin Microbiol"},{"issue":"5","key":"10.1016\/j.diagmicrobio.2013.12.009_bb0050","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1590\/S0036-46652003000500002","article-title":"Human leptospirosis: a twenty-nine-year serological study in S\u00e3o Paulo, Brazil","volume":"45","author":"Romero","year":"2003","journal-title":"Rev Inst Med Trop Sao Paulo"},{"issue":"5","key":"10.1016\/j.diagmicrobio.2013.12.009_bb0055","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1128\/JCM.36.5.1453-1455.1998","article-title":"Detection of Leptospira DNA in patients with aseptic meningitis by PCR","volume":"36","author":"Romero","year":"1998","journal-title":"J Clin Microbiol"},{"issue":"11","key":"10.1016\/j.diagmicrobio.2013.12.009_bb0060","doi-asserted-by":"crossref","first-page":"3940","DOI":"10.1128\/JCM.01119-11","article-title":"Analysis of multilocus sequence typing for identification of Leptospira isolates in Brazil","volume":"49","author":"Romero","year":"2011","journal-title":"J Clin Microbiol"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2013.12.009_bb0065","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1590\/S0036-46651998000300009","article-title":"The persistence of leptospiral agglutinins titers in human sera diagnosed by the microscopic agglutination test","volume":"40","author":"Romero","year":"1998","journal-title":"Rev Inst Med Trop Sao Paulo"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2013.12.009_bb0070","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1590\/S0074-02762006000400005","article-title":"Molecular characterization of Leptospira sp. strains isolated from human subjects in S\u00e3o Paulo, Brazil using a polymerase chain reaction-based assay: a public health tool","volume":"101","author":"Romero","year":"2006","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.diagmicrobio.2013.12.009_bb0075","series-title":"Molecular cloning: a laboratory manual","author":"Sambrook","year":"2001"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2013.12.009_bb0080","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.diagmicrobio.2009.03.014","article-title":"Detection of pathogenic Leptospira spp. through TaqMan polymerase chain reaction targeting the LipL32 gene","volume":"64","author":"Stoddard","year":"2009","journal-title":"Diagn Microbiol Infect Dis"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889313006846?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889313006846?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,8,12]],"date-time":"2020-08-12T19:07:42Z","timestamp":1597259262000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889313006846"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["S0732889313006846"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2013.12.009","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2014,4]]}}}