{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T17:58:15Z","timestamp":1777571895752,"version":"3.51.4"},"reference-count":30,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2015,2]]},"DOI":"10.1016\/j.diagmicrobio.2014.10.013","type":"journal-article","created":{"date-parts":[[2014,11,5]],"date-time":"2014-11-05T08:31:56Z","timestamp":1415176316000},"page":"85-88","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"title":["Detection of the tetM resistance determinant among phenotypically sensitive Ureaplasma species by a novel real-time PCR method"],"prefix":"10.1016","volume":"81","author":[{"given":"Kotrotsiou","family":"Tzimoula","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Exindari","family":"Maria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diza","family":"Eudoxia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gioula","family":"Georgia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melidou","family":"Angeliki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malisiovas","family":"Nikolaos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.diagmicrobio.2014.10.013_bb0005","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1128\/jcm.35.5.1199-1202.1997","article-title":"Association of Ureaplasma urealyticum biovars with clinical outcome for neonates, obstetric patients, and gynecological patients with pelvic inflammatory disease","volume":"35","author":"Abele-Horn","year":"1997","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2014.10.013_bb0155","first-page":"233","article-title":"Increased quinolone-resistant Ureaplasma urealyticum and Mycoplasma hominis in reproductive-age women with vulvovaginitis","volume":"18(s3)","author":"Baka","year":"2012"},{"issue":"5","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0015","doi-asserted-by":"crossref","first-page":"2020","DOI":"10.1128\/AAC.01349-08","article-title":"Concurrent titration and determination of antibiotic resistance in Ureaplasma species with identification of novel point mutations in genes associated with resistance","volume":"53","author":"Beeton","year":"2009","journal-title":"Antimicrob Agents Chemother"},{"issue":"2\u20133","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0020","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1111\/j.1574-6968.1992.tb05379.x","article-title":"Rapid detection of tetM in Mycoplasma hominis and Ureaplasma urealyticum by PCR: tetM confers resistance to tetracycline but not necessarily to doxycycline","volume":"74","author":"Blanchard","year":"1992","journal-title":"FEMS Microbiol Lett"},{"issue":"11","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0025","doi-asserted-by":"crossref","first-page":"1852","DOI":"10.1128\/AAC.31.11.1852","article-title":"Cloning and characterization of tetM gene from a Ureaplasma urealyticum strain","volume":"31","author":"Brown","year":"1987","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0030","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1128\/CMR.6.1.69","article-title":"Ureaplasma urealyticum intrauterine infection: role in prematurity and disease in newborns","volume":"6","author":"Cassell","year":"1993","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.diagmicrobio.2014.10.013_bb0160","series-title":"CLSI document M43-A","article-title":"Methods for antimicrobial susceptibility testing for human mycoplasmas; approved guideline","author":"Clinical and Laboratory Standards Institute (CLSI)","year":"2011"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0040","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1093\/jac\/37.2.223","article-title":"A Tn1545-like transposon carries the tet(M) gene in tetracycline resistant strains of Bacteroides ureolyticus as well as Ureaplasma urealyticum but not Neisseria gonorrhoeae","volume":"37","author":"De Barbeyrac","year":"1996","journal-title":"J Antimicrob Chemother"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0045","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1128\/AAC.00960-07","article-title":"Tetracycline resistance in Ureaplasma spp. and Mycoplasma hominis: prevalence in Bordeaux, France, from 1999 to 2002 and description of two tet(M)-positive isolates of M. hominis susceptible to tetracyclines","volume":"52","author":"D\u00e9grange","year":"2008","journal-title":"Antimicrob Agents Chemother"},{"issue":"11","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0050","doi-asserted-by":"crossref","first-page":"2979","DOI":"10.1128\/AAC.44.11.2979-2984.2000","article-title":"Genetic diversity of the tet(M) gene in tetracycline-resistant clonal lineages of Streptococcus pneumoniae","volume":"44","author":"Doherty","year":"2000","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0055","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1093\/clinids\/17.Supplement_1.S100","article-title":"Ureaplasma urealyticum and premature birth","volume":"17","author":"Eschenbach","year":"1993","journal-title":"Clin Infect Dis"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0060","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1007\/s00248-013-0243-y","article-title":"Antimicrobial resistance, virulence genes, and genetic lineages of Staphylococcus pseudintermedius in healthy dogs in Tunisia","volume":"66","author":"Gharsa","year":"2013","journal-title":"Microb Ecol"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0065","first-page":"19","article-title":"Tetracycline resistance genes of ureaplasmas","volume":"27","author":"Govender","year":"2012","journal-title":"S Afr J Epidemiol Infect"},{"key":"10.1016\/j.diagmicrobio.2014.10.013_bb0165","article-title":"Prevalence and mechanism of antimicrobial resistance in tetracycline-resistant Ureaplasma urealyticum","volume":"3","author":"Guan","year":"2011","journal-title":"Chin J Infect Chemother"},{"issue":"3\u20134","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0075","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.vetmic.2012.06.010","article-title":"Diversity of antimicrobial resistance genes and class-1-integrons in phylogenetically related porcine and human Escherichia coli","volume":"160","author":"H\u00f6lzel","year":"2012","journal-title":"Vet Microbiol"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0080","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1093\/jac\/23.3.341","article-title":"Factors influencing the in-vitro sensitivity of Ureaplasma urealyticum to tetracyclines","volume":"23","author":"Jalil","year":"1989","journal-title":"J Antimicrob Chemother"},{"key":"10.1016\/j.diagmicrobio.2014.10.013_bb0085","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1093\/jac\/dkn158","article-title":"Incidence and antimicrobial susceptibilities of genital mycoplasmas in outpatient women with clinical vaginitis in Athens, Greece","volume":"62","author":"Kechagia","year":"2008","journal-title":"J Antimicrob Chemother"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0090","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1128\/JCM.38.3.1175-1179.2000","article-title":"Species Identification and subtyping of Ureaplasma parvum and Ureaplasma urealyticum using PCR-based assays","volume":"38","author":"Kong","year":"2000","journal-title":"J Clin Microbiol"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0095","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1080\/00365590500199822","article-title":"Mycoplasmas in semen of chronic prostatitis patients","volume":"39","author":"Mandar","year":"2005","journal-title":"Scand J Urol Nephrol"},{"issue":"9","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0100","doi-asserted-by":"crossref","first-page":"1254","DOI":"10.1099\/jmm.0.044016-0","article-title":"Evidence for the predominance of a single tet(M) gene sequence type in tetracycline-resistant Ureaplasma parvum and Mycoplasma hominis isolates from Tunisian patients","volume":"61","author":"Mardassi","year":"2012","journal-title":"J Med Microbiol"},{"key":"10.1016\/j.diagmicrobio.2014.10.013_bb0170","series-title":"Sexually Transmitted Diseases","first-page":"408","article-title":"The genital Mycoplasma","author":"McCormack","year":"1984"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0110","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1097\/INF.0b013e3182791ae0","article-title":"Role of biofilm formation in Ureaplasma antibiotic susceptibility and development of bronchopulmonary dysplasia in preterm neonates","volume":"32","author":"Pandelidis","year":"2013","journal-title":"Pediatr Infect Dis J"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0115","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1128\/AAC.29.2.350","article-title":"Dissemination of the tetM tetracycline resistance determinant to Ureaplasma urealyticum","volume":"29","author":"Roberts","year":"1986","journal-title":"Antimicrob Agents Chemother"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0120","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1093\/jac\/21.3.319","article-title":"Characterization of tetracycline-resistant strains of Ureaplasma urealyticum","volume":"21","author":"Robertson","year":"1988","journal-title":"J Antimicrob Chemother"},{"key":"10.1016\/j.diagmicrobio.2014.10.013_bb0125","first-page":"3","article-title":"Detection of tetracycline- and erythromycin-resistant urogenital Mycoplasma strains using PCR","volume":"6","author":"Solov'eva","year":"1998","journal-title":"Zh Mikrobiol Epidemiol Immunobiol"},{"issue":"11","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0130","first-page":"1463","article-title":"Allelic polymorphism of the tem(M) determinant in Mycoplasma hominis and Ureaplasma urealyticum clinical isolates resistant to tetracyclines","volume":"38","author":"Soroka","year":"2002","journal-title":"Genetika"},{"key":"10.1016\/j.diagmicrobio.2014.10.013_bb0135","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/1471-2334-9-16","article-title":"Human papillomaviruses and genital co-infections in gynaecological outpatients","volume":"9","author":"Verteramo","year":"2009","journal-title":"BMC Infect Dis"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0140","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.clp.2009.12.003","article-title":"Ureaplasma species: role in diseases of prematurity","volume":"37","author":"Viscardi","year":"2010","journal-title":"Clin Perinatol"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0145","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1128\/CMR.18.4.757-789.2005","article-title":"Mycoplasmas and ureaplasmas as neonatal pathogens","volume":"18","author":"Waites","year":"2005","journal-title":"Clin Microbiol Rev"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2014.10.013_bb0150","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.ijantimicag.2010.12.012","article-title":"Mutations in ribosomal proteins and ribosomal RNA confer macrolide resistance in human Ureaplasma spp","volume":"37","author":"Xiao","year":"2011","journal-title":"Int J Antimicrob Agents"}],"updated-by":[{"DOI":"10.1016\/j.diagmicrobio.2015.02.012","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T00:00:00Z","timestamp":1433116800000}}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889314004490?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889314004490?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T09:51:29Z","timestamp":1760003489000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889314004490"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["S0732889314004490"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2014.10.013","relation":{"erratum":[{"id-type":"doi","id":"10.1016\/j.diagmicrobio.2015.02.012","asserted-by":"object"}]},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2015,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Detection of the tetM resistance determinant among phenotypically sensitive Ureaplasma species by a novel real-time PCR method","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2014.10.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}