{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T22:40:47Z","timestamp":1791240047361,"version":"4.1.0"},"reference-count":26,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T00:00:00Z","timestamp":1433116800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["dmidjournal.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2015,6]]},"DOI":"10.1016\/j.diagmicrobio.2015.03.006","type":"journal-article","created":{"date-parts":[[2015,3,12]],"date-time":"2015-03-12T08:22:44Z","timestamp":1426148564000},"page":"143-147","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":37,"title":["Comparison of 2 real-time PCR assays for diagnosis of Pneumocystis jirovecii pneumonia in human immunodeficiency virus (HIV) and non-HIV immunocompromised patients"],"prefix":"10.1016","volume":"82","author":[{"given":"Isabel","family":"Montesinos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Brancart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kinda","family":"Schepers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederique","family":"Jacobs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Denis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Luce","family":"Delforge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0050","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1111\/j.1469-0691.2010.03400.x","article-title":"Real-time PCR assay-based strategy for differentiation between active Pneumocystis jirovecii pneumonia and colonization in immunocompromised patients","volume":"17","author":"Alanio","year":"2011","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0065","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1128\/JCM.06036-11","article-title":"Clinical significance of quantifying Pneumocystis jirovecii DNA by using real-time PCR in bronchoalveolar lavage specimens from immunocompromised patients","volume":"50","author":"Botterel","year":"2012","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0070","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.mimet.2005.01.001","article-title":"Quantitative TaqMan PCR for detection of Pneumocystis jirovecii","volume":"61","author":"Brancart","year":"2005","journal-title":"J Microbiol Methods"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0130","doi-asserted-by":"crossref","first-page":"3380","DOI":"10.1128\/JCM.01554-13","article-title":"Combined quantification of pulmonary Pneumocystis jirovecii DNA and serum (1->3)-beta D-glucan for differential diagnosis of Pneumocystis pneumonia and Pneumocystis colonization","volume":"51","author":"Damiani","year":"2013","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0105","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.femsim.2005.06.006","article-title":"Molecular diagnosis of Pneumocystis pneumonia","volume":"45","author":"Durand-Joly","year":"2005","journal-title":"FEMS Immunol Med Microbiol"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0125","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s10096-012-1730-7","article-title":"Human herpes virus co-infection is associated with mortality in HIV-negative patients with Pneumocystis jirovecii pneumonia","volume":"32","author":"Fillatre","year":"2013","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0045","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1099\/jmm.0.45528-0","article-title":"Comparison between real-time PCR, conventional PCR and different staining techniques for diagnosing Pneumocystis jirovecii pneumonia from bronchoalveolar lavage specimens","volume":"53","author":"Flori","year":"2004","journal-title":"J Med Microbiol"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0040","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1051\/parasite\/2011182121","article-title":"Pneumocystis jirovecii colonization in chronic pulmonary disease","volume":"18","author":"Gutierrez","year":"2011","journal-title":"Parasite"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0110","doi-asserted-by":"crossref","first-page":"1872","DOI":"10.1128\/JCM.02390-10","article-title":"Multicenter, prospective clinical evaluation of respiratory samples from subjects at risk of Pneumocystis jirovecii infection by use of a commercial real-time PCR assay","volume":"49","author":"Hauser","year":"2011","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0090","doi-asserted-by":"crossref","first-page":"S5","DOI":"10.1086\/313843","article-title":"Epidemiology of human immunodeficiency virus\u2013associated opportunistic infections in the United States in the era of highly active antiretroviral therapy","volume":"30","author":"Kaplan","year":"2000","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0085","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.jmii.2012.08.024","article-title":"Pneumocystis jirovecii pneumonia in immunocompromised patients: delayed diagnosis and poor outcomes in non-HIV-infected individuals","volume":"47","author":"Li","year":"2014","journal-title":"J Microbiol Immunol Infect"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0075","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s10096-013-1960-3","article-title":"Pneumocystis jirovecii (PJ) quantitative PCR to differentiate PJ pneumonia from PJ colonization in immunocompromised patients","volume":"33","author":"Maillet","year":"2014","journal-title":"Eur J Clin Microbiol Dis"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0095","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1111\/ajt.12119","article-title":"Pneumocystis pneumonia in solid organ transplantation","volume":"13","author":"Martin","year":"2013","journal-title":"Am J Transplant"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0055","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1111\/j.1469-0691.2011.03605.x","article-title":"Quantitative real-time PCR and the (1-3)-\u03b2-D-glucan assay for differentiation between active Pneumocystis jirovecii pneumonia and colonization","volume":"18","author":"Matsumura","year":"2012","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0120","doi-asserted-by":"crossref","first-page":"1856","DOI":"10.1128\/JCM.05880-11","article-title":"Validation of the MycAssay Pneumocystis Kit for detection of Pneumocystis jirovecii in bronchoalveolar lavage specimens in comparison to a laboratory standard of direct immunofluorescence microscopy, real-time PCR, or conventional PCR","volume":"50","author":"McTaggart","year":"2012","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0035","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1086\/523814","article-title":"Epidemiology and clinical significance of Pneumocystis colonization","volume":"197","author":"Morris","year":"2008","journal-title":"J Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0020","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1086\/313534","article-title":"Pneumocystis carinii pneumonia in human immunodeficiency virus (HIV)-positive and HIV-negative immunocompromised patients","volume":"29","author":"N\u00fcesch","year":"1999","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0115","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.diagmicrobio.2012.03.001","article-title":"Performance of 2 commercial real-time PCR assays for the detection of Aspergillus and Pneumocystis DNA in bronchoalveolar lavage fluid samples from critical care patients","volume":"73","author":"Orsi","year":"2012","journal-title":"Diagnostic Microbiol Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0100","doi-asserted-by":"crossref","first-page":"3333","DOI":"10.1128\/JCM.42.7.3333-3335.2004","article-title":"Detection of Pneumocystis jirovecii in respiratory specimens by four staining methods","volume":"42","author":"Procop","year":"2004","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0030","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1128\/JCM.35.4.830-835.1997","article-title":"PCR detection of Pneumocystis carinii in bronchoalveolar lavage specimens: analysis of sensitivity and specificity","volume":"35","author":"Ribes","year":"1997","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0080","doi-asserted-by":"crossref","first-page":"3370","DOI":"10.1128\/JCM.01480-14","article-title":"Diagnosis of Pneumocystis carinii pneumonia in immunocompromised patients by real-time PCR: a 4 year prospective study","volume":"52","author":"Robert-Gangneux","year":"2014","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0015","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1007\/s10096-002-0758-5","article-title":"Analysis of underlying diseases and prognosis factors associated with Pneumocystis carinii pneumonia in immunocompromised HIV-negative patients","volume":"21","author":"Roblot","year":"2002","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0025","doi-asserted-by":"crossref","first-page":"3409","DOI":"10.1128\/JCM.37.10.3409-3410.1999","article-title":"Pneumocystis carinii carriage in immunocompetent patients with primary pulmonary disorders as detected by single or nested PCR","volume":"32","author":"Sing","year":"1999","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0010","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1007\/s10156-012-0453-0","article-title":"Pneumocystis jirovecii pneumonia in non-HIV-infected patients in the era of novel immunosuppressive therapies","volume":"18","author":"Tasaka","year":"2012","journal-title":"J Infect Chemother"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0060","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1111\/j.1469-0691.2011.03656.x","article-title":"A highly sensitive novel PCR assay for detection of Pneumocystis jirovecii DNA in bronchoalveolar lavage specimens from immunocompromised patients","volume":"18","author":"Tia","year":"2012","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.diagmicrobio.2015.03.006_bb0005","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1086\/526778","article-title":"Early predictors of mortality from Pneumocystis jirovecii pneumonia in HIV-infected patients: 1985\u20132006","volume":"46","author":"Walzer","year":"2008","journal-title":"Clin Infect Dis"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889315000723?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889315000723?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,8,30]],"date-time":"2020-08-30T19:35:00Z","timestamp":1598816100000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889315000723"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,6]]}},"alternative-id":["S0732889315000723"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2015.03.006","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2015,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Comparison of 2 real-time PCR assays for diagnosis of Pneumocystis jirovecii pneumonia in human immunodeficiency virus (HIV) and non-HIV immunocompromised patients","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2015.03.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}