{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:13:31Z","timestamp":1790669611079,"version":"4.1.0"},"reference-count":39,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,3,31]],"date-time":"2016-03-31T00:00:00Z","timestamp":1459382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","clinicalkey.fr","dmidjournal.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2016,7]]},"DOI":"10.1016\/j.diagmicrobio.2016.03.022","type":"journal-article","created":{"date-parts":[[2016,4,3]],"date-time":"2016-04-03T18:28:37Z","timestamp":1459708117000},"page":"295-301","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":31,"title":["Simultaneous detection of Legionella species and L. anisa , L. bozemanii , L. longbeachae and L. micdadei using conserved primers and multiple probes in a multiplex real-time PCR assay"],"prefix":"10.1016","volume":"85","author":[{"given":"Kristen E.","family":"Cross","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey W.","family":"Mercante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvaro J.","family":"Benitez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen W.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maureen H.","family":"Diaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas M.","family":"Winchell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"9","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0045","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1086\/342903","article-title":"Trends in legionnaires disease, 1980\u20131998: declining mortality and new patterns of diagnosis","volume":"35","author":"Benin","year":"2002","journal-title":"Clin Infect Dis"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0125","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1128\/JCM.02510-12","article-title":"Clinical application of a multiplex real-time PCR assay for simultaneous detection of Legionella species, Legionella pneumophila, and Legionella pneumophila serogroup 1","volume":"51","author":"Benitez","year":"2013","journal-title":"J Clin Microbiol"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0130","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.diagmicrobio.2016.01.007","article-title":"Rapid detection and typing of pathogenic non-pneumophila Legionella spp. isolates using a multiplex real-time PCR assay","volume":"84","author":"Benitez","year":"2016","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"34","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0195","first-page":"777","article-title":"Legionnaires' Disease associated with potting soil--California, Oregon, and Washington, May-June 2000","volume":"49","author":"Centers for Disease Control and Prevention (CDC)","year":"2000","journal-title":"MMWR Morb Mortal Wkly Rep"},{"issue":"5","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0105","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1128\/JCM.38.5.1709-1712.2000","article-title":"Detection of Legionella Species in Respiratory Specimens Using PCR with Sequencing Confirmation","volume":"38","author":"Cloud","year":"2000","journal-title":"J Clin Microbiol"},{"issue":"12","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0085","first-page":"871","article-title":"Diagnosis of Legionella infection in Legionnaires' disease","volume":"23","author":"Den Boer","year":"2004","journal-title":"Eur J Clin Microbiol Infect Dis"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0120","doi-asserted-by":"crossref","first-page":"e66183","DOI":"10.1371\/journal.pone.0066183","article-title":"Optimization of Multiple Pathogen Detection Using the TaqMan Array Card: Application for a Population-Based Study of Neonatal Infection","volume":"8","author":"Diaz","year":"2013","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0070","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jinf.2007.09.010","article-title":"Legionella spp. and Legionnaires' disease","volume":"56","author":"Diederen","year":"2008","journal-title":"J Infect"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0145","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/0195-6701(89)90010-8","article-title":"Nosocomial Legionella micdadei pneumonia: 10years experience and a case\u2013control study","volume":"13","author":"Doebbeling","year":"1989","journal-title":"J Hosp Infect"},{"issue":"9","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0090","doi-asserted-by":"crossref","first-page":"2718","DOI":"10.1128\/JCM.36.9.2718-2722.1998","article-title":"Comparison of the Binax Legionella urinary antigen enzyme immunoassay (EIA) with the Biotest Legionella Urin antigen EIA for detection of Legionella antigen in both concentrated and nonconcentrated urine samples","volume":"36","author":"Dominguez","year":"1998","journal-title":"J Clin Microbiol"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0140","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1097\/00005792-198903000-00005","article-title":"Disease due to the Legionellaceae (other than Legionella pneumophila). Historical, microbiological, clinical, and epidemiological review","volume":"68","author":"Fang","year":"1989","journal-title":"Medicine"},{"issue":"7","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0005","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/0966-842X(96)10041-X","article-title":"The molecular ecology of legionellae","volume":"4","author":"Fields","year":"1996","journal-title":"Trends Microbiol"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0010","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1128\/CMR.15.3.506-526.2002","article-title":"Legionella and Legionnaires' Disease: 25 Years of Investigation","volume":"15","author":"Fields","year":"2002","journal-title":"Clin Microbiol Rev"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0030","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/S0891-5520(05)70199-9","article-title":"The role of atypical pathogens: Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella pneumophila in respiratory infection","volume":"12","author":"File","year":"1998","journal-title":"Infect Dis Clin North Am"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0115","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.micinf.2005.05.022","article-title":"Analysis of the genetic diversity of Legionella by sequencing the 23S-5S ribosomal intergenic spacer region: from phylogeny to direct identification of isolates at the species level from clinical specimens","volume":"8","author":"Grattard","year":"2006","journal-title":"Microbes Infect"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0170","first-page":"E313","article-title":"Australia's notifiable disease status, 2011: annual report of the National Notifiable Diseases Surveillance System","volume":"37","author":"Group NARW","year":"2013","journal-title":"Commun Dis Intell Q Rep"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0150","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/0163-4453(92)93561-4","article-title":"Pneumonia due to Legionella bozemanii and Chlamydia psittaci\/TWAR following renal transplantation","volume":"25","author":"Humphreys","year":"1992","journal-title":"J Infect"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0155","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/S0002-9343(99)00459-3","article-title":"An outbreak of Legionella micdadei pneumonia in transplant patients: evaluation, molecular epidemiology, and control","volume":"108","author":"Knirsch","year":"2000","journal-title":"Am J Med"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0175","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1007\/s101560170017","article-title":"Distribution of Legionella longbeachae and other legionellae in Japanese potting soils","volume":"7","author":"Koide","year":"2001","journal-title":"J Infect Chemother"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0065","doi-asserted-by":"crossref","first-page":"2764","DOI":"10.1128\/JCM.31.10.2764-2768.1993","article-title":"Growth of 28 Legionella species on selective culture media: a comparative study","volume":"31","author":"Lee","year":"1993","journal-title":"J Clin Microbiol"},{"issue":"12","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0095","doi-asserted-by":"crossref","first-page":"905","DOI":"10.2307\/30142015","article-title":"A recurrent outbreak of nosocomial legionnaires' disease detected by urinary antigen testing: evidence for long-term colonization of a hospital plumbing system","volume":"19","author":"Lepine","year":"1998","journal-title":"Infect Control Hosp Epidemiol"},{"issue":"21","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0135","doi-asserted-by":"crossref","first-page":"2417","DOI":"10.1001\/archinte.1994.00420210049006","article-title":"Surveillance for Legionnaires' disease. Risk factors for morbidity and mortality","volume":"154","author":"Marston","year":"1994","journal-title":"Arch Intern Med"},{"key":"10.1016\/j.diagmicrobio.2016.03.022_bb0025","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1001\/archinte.1997.00440360129015","article-title":"Incidence of community-acquired pneumonia requiring hospitalization. Results of a population-based active surveillance Study in Ohio. The Community-Based Pneumonia Incidence Study Group","volume":"157","author":"Marston","year":"1997","journal-title":"Arch Intern Med"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0055","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0732-8893(00)00181-4","article-title":"Potential importance of Legionella species as etiologies in community acquired pneumonia (CAP)","volume":"38","author":"McNally","year":"2000","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0015","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1128\/CMR.00029-14","article-title":"Current and emerging Legionella diagnostics for laboratory and outbreak investigations","volume":"28","author":"Mercante","year":"2015","journal-title":"Clin Microbiol Rev"},{"issue":"8","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0020","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1086\/342884","article-title":"Infection due to Legionella species other than L. pneumophila","volume":"35","author":"Muder","year":"2002","journal-title":"Clin Infect Dis"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0075","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1086\/345529","article-title":"Diagnosis of Legionella Infection","volume":"36","author":"Murdoch","year":"2003","journal-title":"Clin Infect Dis"},{"issue":"9","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0080","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1093\/cid\/cit504","article-title":"Impact of routine systematic polymerase chain reaction testing on case finding for Legionnaires' disease: a pre-post comparison study","volume":"57","author":"Murdoch","year":"2013","journal-title":"Clin Infect Dis"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0160","doi-asserted-by":"crossref","first-page":"205","DOI":"10.7326\/0003-4819-103-2-205","article-title":"Waterborne Legionella bozemanii and nosocomial pneumonia in immunosuppressed patients","volume":"103","author":"Parry","year":"1985","journal-title":"Ann Intern Med"},{"issue":"7","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0180","doi-asserted-by":"crossref","first-page":"1368","DOI":"10.1016\/j.meegid.2012.03.025","article-title":"Genetic speciation of environmental Legionella isolates in Thailand","volume":"12","author":"Paveenkittiporn","year":"2012","journal-title":"Infect Genet Evol"},{"issue":"12","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0165","doi-asserted-by":"crossref","first-page":"e147","DOI":"10.1086\/523003","article-title":"Epidemiology of severe pneumonia caused by Legionella longbeachae, Mycoplasma pneumoniae, and Chlamydia pneumoniae: 1-year, population-based surveillance for severe pneumonia in Thailand","volume":"45","author":"Phares","year":"2007","journal-title":"Clin Infect Dis"},{"issue":"8","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0185","doi-asserted-by":"crossref","DOI":"10.2807\/ese.15.08.19496-en","article-title":"A cluster of Legionnaires' disease caused by Legionella longbeachae linked to potting compost in Scotland, 2008\u20132009","volume":"15","author":"Pravinkumar","year":"2010","journal-title":"Euro Surveill"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0100","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1128\/JCM.36.6.1560-1567.1998","article-title":"Sequence-Based Classification Scheme for the Genus Legionella Targeting the mip Gene","volume":"36","author":"Ratcliff","year":"1998","journal-title":"J Clin Microbiol"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0035","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1056\/NEJM199709043371006","article-title":"Legionellosis","volume":"337","author":"Stout","year":"1997","journal-title":"N Engl J Med"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0110","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1111\/j.1469-0691.2011.03698.x","article-title":"The occurrence of Legionella species other than Legionella pneumophila in clinical and environmental samples in Denmark identified by mip gene sequencing and matrix-assisted laser desorption ionization time-of-flight mass spectrometry","volume":"18","author":"Svarrer","year":"2012","journal-title":"Clin Microbiol Infect"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0060","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1128\/JCM.44.1.56-59.2006","article-title":"Legionella anisa, a possible indicator of water contamination by Legionella pneumophila","volume":"44","author":"van der Mee-Marquet","year":"2006","journal-title":"J Clin Microbiol"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0040","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0002-9343(00)00624-0","article-title":"Legionella and community-acquired pneumonia: a review of current diagnostic tests from a clinician's viewpoint","volume":"110","author":"Waterer","year":"2001","journal-title":"Am J Med"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0190","doi-asserted-by":"crossref","first-page":"579","DOI":"10.3201\/eid1704.100446","article-title":"Legionella longbeachae and legionellosis","volume":"17","author":"Whiley","year":"2011","journal-title":"Emerg Infect Dis"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2016.03.022_bb0050","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1086\/341087","article-title":"Distribution of Legionella Species and Serogroups Isolated by Culture in Patients with Sporadic Community\u2212Acquired Legionellosis: An International Collaborative Survey","volume":"186","author":"Yu","year":"2002","journal-title":"J Infect Dis"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889316300761?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889316300761?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T03:37:49Z","timestamp":1600400269000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889316300761"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,7]]}},"alternative-id":["S0732889316300761"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2016.03.022","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2016,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Simultaneous detection of Legionella species and L. anisa, L. bozemanii, L. longbeachae and L. micdadei using conserved primers and multiple probes in a multiplex real-time PCR assay","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2016.03.022","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}