{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T15:07:46Z","timestamp":1791990466706,"version":"4.4.0"},"reference-count":21,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2020,6]]},"DOI":"10.1016\/j.diagmicrobio.2020.115037","type":"journal-article","created":{"date-parts":[[2020,3,12]],"date-time":"2020-03-12T04:11:36Z","timestamp":1583986296000},"page":"115037","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":47,"title":["Expanded sequential quadriplex real-time polymerase chain reaction (PCR) for identifying pneumococcal serotypes, penicillin susceptibility, and resistance markers"],"prefix":"10.1016","volume":"97","author":[{"given":"Srinivasan","family":"Velusamy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa","family":"Tran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thitipong","family":"Mongkolrattanothai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hollis","family":"Walker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lesley","family":"McGee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Beall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.diagmicrobio.2020.115037_bb0005","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0009282","article-title":"Realtime PCR is more sensitive than multiplex PCR for diagnosis and serotyping in children with culture negative pneumococcal invasive disease","volume":"5","author":"Azzari","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0010","doi-asserted-by":"crossref","unstructured":"Beall B, Chochua S, Gertz Jr RE, Li Y, Li Z, McGee L, et al. A population-based description atlas of invasive pneumococcal strains recovered within the U.S. during 2015\u20132016. Front Microbiol 2018; Nov 19;9:2670. doi: https:\/\/doi.org\/10.3389\/fmicb.2018.02670. [eCollection 2018].","DOI":"10.3389\/fmicb.2018.02670"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2020.115037_bb0015","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1097\/INF.0b013e3182002d14","article-title":"Molecular analysis improves pathogen identification and epidemiologic study of pediatric parapneumonic empyema","volume":"30","author":"Blaschke","year":"2011","journal-title":"Pediatr Infect Dis J"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0020","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1128\/JCM.02498-06","article-title":"Evaluation and improvement of real-time PCR assays targeting lytA, ply, and psaA genes for detection of pneumococcal DNA","volume":"45","author":"Carvalho","year":"2007","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0025","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1128\/JCM.02243-09","article-title":"Revisiting pneumococcal carriage using broth-enrichment and PCR techniques for enhanced detection of carriage and serotypes","volume":"48","author":"Carvalho","year":"2010","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0030","doi-asserted-by":"crossref","first-page":"2775","DOI":"10.1128\/JCM.00822-17","article-title":"WciG O-Acetyltransferase functionality differentiates pneumococcal serotypes 35C and 42","volume":"55","author":"Geno","year":"2017","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0035","doi-asserted-by":"crossref","first-page":"2470","DOI":"10.1128\/JCM.00484-09","article-title":"Simple, accurate, serotype-specific PCR assay to discriminate Streptococcus pneumoniae serotypes, 6A, 6B and 6C","volume":"47","author":"Jin","year":"2009","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0040","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/978-0-387-79838-7_11","article-title":"The significance of serotype replacement for pneumococcal disease and antibiotic resistance","volume":"634","author":"Klugman","year":"2009","journal-title":"Adv Exp Med Biol"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0045","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1128\/JCM.05987-11","article-title":"Engineered combined-positive-control template for real-time reverse transcription-PCR in multiple-pathogen-detection assays","volume":"50","author":"Kodani","year":"2011","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0050","doi-asserted-by":"crossref","first-page":"1887","DOI":"10.1128\/JCM.44.5.1887-1891.2006","article-title":"Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes","volume":"44","author":"Kong","year":"2006","journal-title":"J Clin Microbiol"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2020.115037_bb0055","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1089\/mdr.2011.0031","article-title":"Detection of Streptococcus pneumoniae and identification of pneumococcal serotypes by real-time polymerase chain reaction using blood samples from Italian children \u2264 5 years of age with community-acquired pneumonia","volume":"17","author":"Marchese","year":"2011","journal-title":"Microb Drug Resist"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0060","doi-asserted-by":"crossref","first-page":"451","DOI":"10.4269\/ajtmh.2010.10-0225","article-title":"Enhanced determination of Streptococcus pneumoniae serotypes associated with invasive disease in Laos by using a real-time polymerase chain reaction serotyping assay with cerebrospinal fluid","volume":"83","author":"Moore","year":"2010","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0065","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1128\/JCM.44.1.124-131.2006","article-title":"Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates","volume":"44","author":"Pai","year":"2006","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0070","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1128\/JCM.02927-12","article-title":"Sequential triplex real-time PCR assay for detecting 21 pneumococcal capsular serotypes that account for a high global disease burden","volume":"51","author":"Pimenta","year":"2013","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0075","doi-asserted-by":"crossref","unstructured":"Sakai F, Chochua S, Satzke C, Dunne EM, Mulholland K, Klugman KP et al. Single-plex quantitative assays for the detection and quantification of most pneumococcal serotypes. PLoS One 2015; Mar 23;10(3):e0121064. doi: https:\/\/doi.org\/10.1371\/journal.pone.0121064.","DOI":"10.1371\/journal.pone.0121064"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0080","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.plasmid.2018.09.005","article-title":"Multi-target plasmid controls for conventional and real-time PCR-based serotyping of Streptococcus pneumoniae","volume":"98","author":"Schembri","year":"2018","journal-title":"Plasmid"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0085","doi-asserted-by":"crossref","unstructured":"Shak JR, Vidal JE, Klugman KP. Influence of bacterial interactions on pneumococcal colonization of the nasopharynx. Trends in microbiology. 2013; 21(3):129\u201335. Epub 2013\/01\/01. doi: https:\/\/doi.org\/10.1016\/j.tim. 2012.11.005 PMID: 23273566","DOI":"10.1016\/j.tim.2012.11.005"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0090","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.diagmicrobio.2011.08.017","article-title":"Quadriplex real-time polymerase chain reaction (lytA, mef, erm, pbp2b(wt)) for pneumococcal detection and assessment of antibiotic susceptibility","volume":"71","author":"Srinivasan","year":"2011","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0095","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1111\/j.1469-0691.2008.02028.x","article-title":"Identification of pneumococcal serotypes from culture-negative clinical specimens by novel real-time PCR","volume":"14","author":"Tarrag\u00f3","year":"2008","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0100","doi-asserted-by":"crossref","unstructured":"Varghese J, Chochua S, Tran T, Walker H, Li Z, Snippes Vagnone PM, et al. Multistate population and whole genome sequence-based strain surveillance of invasive pneumococci recovered in the USA during 2017. Clin Microbiol Infect 2019; Sep 16. pii: S1198-743X(19)30493\u20138. doi: https:\/\/doi.org\/10.1016\/j.cmi.2019.09.008.","DOI":"10.1016\/j.cmi.2019.09.008"},{"key":"10.1016\/j.diagmicrobio.2020.115037_bb0105","doi-asserted-by":"crossref","first-page":"3411","DOI":"10.1128\/JCM.01076-07","article-title":"Identification of lesscommon Streptococcus pneumoniae serotypes by a multiplex PCR-based reverse line blot hybridization assay","volume":"45","author":"Zhou","year":"2007","journal-title":"J Clin Microbiol"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889320300171?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889320300171?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T09:53:41Z","timestamp":1760003621000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889320300171"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6]]},"references-count":21,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,6]]}},"alternative-id":["S0732889320300171"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2020.115037","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2020,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Expanded sequential quadriplex real-time polymerase chain reaction (PCR) for identifying pneumococcal serotypes, penicillin susceptibility, and resistance markers","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2020.115037","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"115037"}}