{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T23:02:03Z","timestamp":1776466923188,"version":"3.51.2"},"reference-count":11,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2021,1]]},"DOI":"10.1016\/j.diagmicrobio.2020.115206","type":"journal-article","created":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T23:53:45Z","timestamp":1599868425000},"page":"115206","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"title":["Validation of SARS CoV-2 detection by real-time PCR in matched pooled and deconvoluted clinical samples before and after nucleic acid extraction: a study in tertiary care hospital of North India"],"prefix":"10.1016","volume":"99","author":[{"given":"Komal","family":"Chhikara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Poonam","family":"Kanta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnab","family":"Ghosh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rishi Chetanya","family":"Prakash","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kapil","family":"Goyal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mini P","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.diagmicrobio.2020.115206_bb0005","article-title":"Saliva is a reliable tool to detect SARS-CoV-2","volume":"14","author":"Azzi","year":"2020","journal-title":"J Infect"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2020.115206_bb0010","doi-asserted-by":"crossref","first-page":"35","DOI":"10.4103\/0973-6247.126688","article-title":"Half a decade of mini-pool nucleic acid testing: cost-effective way for improving blood safety in India","volume":"8","author":"Chandrashekar","year":"2014","journal-title":"Asian journal of transfusion science"},{"key":"10.1016\/j.diagmicrobio.2020.115206_bb0015","article-title":"The presence of SARS-CoV-2 RNA in the feces of COVID-19 patients","volume":"3","author":"Chen","year":"2020","journal-title":"J Med Virol"},{"issue":"10239","key":"10.1016\/j.diagmicrobio.2020.115206_bb0020","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1016\/S0140-6736(20)31181-8","article-title":"Detection of SARS-CoV-2 in human breast milk","volume":"395","author":"Gro\u00df","year":"2020","journal-title":"The Lancet"},{"key":"10.1016\/j.diagmicrobio.2020.115206_bb0025","article-title":"Sample pooling as a strategy to detect community transmission of SARS-CoV-2","volume":"6","author":"Hogan","year":"2020","journal-title":"JAMA"},{"issue":"8","key":"10.1016\/j.diagmicrobio.2020.115206_bb0030","doi-asserted-by":"crossref","first-page":"1558","DOI":"10.1111\/j.1537-2995.2008.01718.x","article-title":"Experience of German Red Cross blood donor services with nucleic acid testing: results of screening more than 30 million blood donations for human immunodeficiency virus-1, hepatitis C virus, and hepatitis B virus","volume":"48","author":"Hourfar","year":"2008","journal-title":"Transfusion"},{"issue":"11","key":"10.1016\/j.diagmicrobio.2020.115206_bb0035","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1111\/jfd.13083","article-title":"To pool or not to pool? Guidelines for pooling samples for use in surveillance testing of infectious diseases in aquatic animals","volume":"42","author":"Laurin","year":"2019","journal-title":"J Fish Dis"},{"key":"10.1016\/j.diagmicrobio.2020.115206_bb0040","doi-asserted-by":"crossref","first-page":"110450","DOI":"10.1016\/j.jtbi.2020.110450","article-title":"Rapid, large-scale, and effective detection of COVID-19 via non-adaptive testing","volume":"506","author":"T\u00e4ufer","year":"2020","journal-title":"J Theor Biol"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2020.115206_bb0045","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1128\/JCM.05631-11","article-title":"Pooling nasopharyngeal\/throat swab specimens to increase testing capacity for influenza viruses by PCR","volume":"50","author":"Van","year":"2012","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2020.115206_bb0050","first-page":"ciaa531","article-title":"Evaluation of COVID-19 RT-qPCR test in multi-sample pools","author":"Yelin","year":"2020","journal-title":"Clin Infect Dis"},{"issue":"7","key":"10.1016\/j.diagmicrobio.2020.115206_bb0055","doi-asserted-by":"crossref","first-page":"2211","DOI":"10.1111\/trf.15296","article-title":"Predictive model for Zika virus RNA minipool nucleic acid testing in outbreak scenarios","volume":"59","author":"Yogurtcu","year":"2019","journal-title":"Transfusion"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889320305836?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889320305836?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T09:55:12Z","timestamp":1760003712000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889320305836"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["S0732889320305836"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2020.115206","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Validation of SARS CoV-2 detection by real-time PCR in matched pooled and deconvoluted clinical samples before and after nucleic acid extraction: a study in tertiary care hospital of North India","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2020.115206","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"115206"}}