{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T10:10:25Z","timestamp":1760004625470,"version":"build-2065373602"},"reference-count":12,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2021,7]]},"DOI":"10.1016\/j.diagmicrobio.2021.115366","type":"journal-article","created":{"date-parts":[[2021,3,5]],"date-time":"2021-03-05T19:24:20Z","timestamp":1614972260000},"page":"115366","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":15,"title":["Caution in interpretation of SARS-CoV-2 quantification based on RT-PCR cycle threshold value"],"prefix":"10.1016","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0931-8720","authenticated-orcid":false,"given":"Aur\u00e9lie","family":"Schnuriger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marine","family":"Perrier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Val\u00e9rie","family":"Marinho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanne","family":"Michel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenda","family":"Saloum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narjis","family":"Boukli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sidonie","family":"Lambert-Niclot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corinne","family":"Amiel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Djeneba Bocar","family":"Fofana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4073-1866","authenticated-orcid":false,"given":"Jo\u00ebl","family":"Gozlan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurence","family":"Morand-Joubert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.diagmicrobio.2021.115366_bib0001","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045","article-title":"Detection of 2019 novel coronavirus (2019-NCoV) by real-time RT-PCR","volume":"25","author":"Corman","year":"2020","journal-title":"Eurosurveillance"},{"key":"10.1016\/j.diagmicrobio.2021.115366_bib0002","doi-asserted-by":"crossref","DOI":"10.1016\/j.jviromet.2020.113970","article-title":"Comparison of commercial assays and laboratory developed tests for detection of SARS-CoV-2","volume":"285","author":"Dust","year":"2020","journal-title":"J Virol Methods"},{"key":"10.1016\/j.diagmicrobio.2021.115366_bib0003","article-title":"RT-PCR for SARS-CoV-2: quantitative versus qualitative","author":"Han","year":"2020","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2021.115366_bib0004","article-title":"Comparison of seven commercial RT-PCR diagnostic kits for COVID-19","volume":"128","author":"Kasteren","year":"2020","journal-title":"J Clin Virol"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2021.115366_bib0005","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1093\/clinchem\/hvaa091","article-title":"Potential false-negative nucleic acid testing results for severe acute respiratory syndrome coronavirus 2 from thermal inactivation of samples with low viral loads","volume":"66","author":"Pan","year":"2020","journal-title":"Clin Chem"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2021.115366_bib0006","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1177\/0194599820931793","article-title":"Inappropriate nasopharyngeal sampling for SARS-CoV-2 detection is a relevant cause of false-negative reports","volume":"163","author":"Piras","year":"2020","journal-title":"Otolaryngol Head Neck Surg"},{"key":"10.1016\/j.diagmicrobio.2021.115366_bib0007","article-title":"A comparison of five SARS-CoV-2 molecular assays with clinical correlations","author":"Procop","year":"2020","journal-title":"Am J Clin Pathol"},{"issue":"22","key":"10.1016\/j.diagmicrobio.2021.115366_bib0008","doi-asserted-by":"crossref","first-page":"2249","DOI":"10.1001\/jama.2020.8259","article-title":"Interpreting diagnostic tests for SARS-CoV-2","volume":"323","author":"Sethuraman","year":"2020","journal-title":"JAMA"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2021.115366_bib0009","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1038\/s41564-020-0761-6","article-title":"Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT\u2013QPCR Primer\u2013Probe Sets","volume":"5","author":"Vogels","year":"2020","journal-title":"Nat Microbiol"},{"key":"10.1016\/j.diagmicrobio.2021.115366_bib0010","doi-asserted-by":"crossref","DOI":"10.1016\/j.jinf.2020.10.009","article-title":"The duration of infectiousness of individuals infected with SARS-CoV-2","author":"Walsh","year":"2020","journal-title":"J Infect"},{"key":"10.1016\/j.diagmicrobio.2021.115366_bib0011","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jcv.2017.02.010","article-title":"Pitfalls in interpretation of CT-values of RT-PCR in children with acute respiratory tract infections","volume":"90","author":"Wishaupt","year":"2017","journal-title":"J Clin Virol"},{"issue":"8","key":"10.1016\/j.diagmicrobio.2021.115366_bib0012","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00743-20","article-title":"Comparison of four molecular in vitro diagnostic assays for the detection of SARS-CoV-2 in nasopharyngeal specimens","volume":"58","author":"Zhen","year":"2020","journal-title":"J Clin Microbiol"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889321000596?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889321000596?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T09:55:33Z","timestamp":1760003733000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889321000596"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7]]},"references-count":12,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["S0732889321000596"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2021.115366","relation":{},"ISSN":["0732-8893"],"issn-type":[{"type":"print","value":"0732-8893"}],"subject":[],"published":{"date-parts":[[2021,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Caution in interpretation of SARS-CoV-2 quantification based on RT-PCR cycle threshold value","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2021.115366","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"115366"}}