{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T23:29:03Z","timestamp":1787614143393,"version":"build-2736575974"},"reference-count":29,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2021,8]]},"DOI":"10.1016\/j.diagmicrobio.2021.115390","type":"journal-article","created":{"date-parts":[[2021,5,12]],"date-time":"2021-05-12T13:51:02Z","timestamp":1620827462000},"page":"115390","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"title":["Microbiological investigation of samples collected from healthy middle ears during cochlear implant surgery"],"prefix":"10.1016","volume":"100","author":[{"given":"Mahmut Tayyar","family":"Kalcioglu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u0131za","family":"Durmaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oguz","family":"Ari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serdal","family":"Celik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sema","family":"Karabudak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.diagmicrobio.2021.115390_bib0001","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1186\/s12866-017-1101-8","article-title":"A comparison of sequencing platforms and bioinformatics pipelines for compositional analysis of the gut microbiome","volume":"17","author":"Allali","year":"2017","journal-title":"BMC Microbiol"},{"key":"10.1016\/j.diagmicrobio.2021.115390_bib0002","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/2049-2618-2-22","article-title":"Characterization of the nasopharyngeal microbiota in health and during rhinovirus challenge","volume":"2","author":"Allen","year":"2014","journal-title":"Microbiome"},{"key":"10.1016\/j.diagmicrobio.2021.115390_bib0003","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijporl.2019.109624","article-title":"The bacteriome of otitis media with effusion: does it originate from the adenoid?","volume":"126","author":"Ari","year":"2019","journal-title":"Int J Pediatr Otorhinolaryngol"},{"key":"10.1016\/j.diagmicrobio.2021.115390_bib0004","doi-asserted-by":"crossref","first-page":"555","DOI":"10.3389\/fgene.2019.00555","article-title":"Age-dependent dissimilarity of the nasopharyngeal and middle ear microbiota in children with acute otitis media","volume":"10","author":"Brugger","year":"2019","journal-title":"Front Genet"},{"key":"10.1016\/j.diagmicrobio.2021.115390_bib0005","first-page":"265","article-title":"Nonparametric estimation of the classes in a population","volume":"11","author":"Chao","year":"1984","journal-title":"Scand J Statistics"},{"key":"10.1016\/j.diagmicrobio.2021.115390_bib0006","doi-asserted-by":"crossref","DOI":"10.1128\/msystems.00169-18","article-title":"The adenoids but not the palatine tonsils serve as a reservoir for bacteria associated with secretory otitis media in small children","volume":"4","author":"Fag\u00f6-Olsen","year":"2019","journal-title":"mSystems"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2021.115390_bib0007","doi-asserted-by":"crossref","first-page":"e17985","DOI":"10.5812\/jjm.17985","article-title":"Identification of Alloiococcus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in children with otitis media with effusion","volume":"8","author":"Farajzadah Sheikh","year":"2015","journal-title":"Jundishapur J Microbiol"},{"key":"10.1016\/j.diagmicrobio.2021.115390_bib0008","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1001\/jama.296.2.202","article-title":"Direct detection of bacterial biofilms on the middle-ear mucosa of children with chronic otitis media","volume":"296","author":"Hall-Stoodley","year":"2006","journal-title":"JAMA"},{"key":"10.1016\/j.diagmicrobio.2021.115390_bib0009","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1097\/INF.0000000000001500","article-title":"Nasal airway microbiota profile and severe bronchiolitis in infants: a case-control study","volume":"36","author":"Hasegawa","year":"2017","journal-title":"Pediatr Infect Dis J"},{"key":"10.1016\/j.diagmicrobio.2021.115390_bib0010","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/1471-2431-12-87","article-title":"One third of middle ear effusions from children undergoing tympanostomy tube placement had multiple bacterial pathogens","volume":"12","author":"Holder","year":"2012","journal-title":"BMC Pediatr"},{"key":"10.1016\/j.diagmicrobio.2021.115390_bib0011","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00456-19","article-title":"Examining the evidence for an adult healthy middle ear microbiome","volume":"4","author":"Jervis-Bardy","year":"2019","journal-title":"mSphere"},{"key":"10.1016\/j.diagmicrobio.2021.115390_bib0012","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.1016\/j.ijporl.2015.07.013","article-title":"The microbiome of otitis media with effusion in Indigenous Australian children","volume":"79","author":"Jervis-Bardy","year":"2015","journal-title":"Int J Pediatr Otorhinolaryngol"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2021.115390_bib0013","first-page":"31","article-title":"Bacterial etiology of otitis media with effusion; focusing on the high positivity of Alloiococcus otitidis","volume":"25","author":"Kalcioglu","year":"2002","journal-title":"New Microbiol"},{"issue":"8","key":"10.1016\/j.diagmicrobio.2021.115390_bib0014","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.1099\/jmm.0.001003","article-title":"Analysis of the effect of smoking on the buccal microbiome using next-generation sequencing technology","volume":"68","author":"Karabudak","year":"2019","journal-title":"J Med Microbiol"},{"key":"10.1016\/j.diagmicrobio.2021.115390_bib0015","doi-asserted-by":"crossref","first-page":"339","DOI":"10.3389\/fcimb.2019.00339","article-title":"Altered middle ear microbiome in children with chronic otitis media with effusion and respiratory Illnesses","volume":"9","author":"Kolbe","year":"2019","journal-title":"Front Cell Infect Microbiol"},{"issue":"Suppl 1","key":"10.1016\/j.diagmicrobio.2021.115390_bib0016","article-title":"Recent advances in understanding the natural history of the otitis media microbiome and its response to environmental pressures","volume":"130","author":"Marsh","year":"2020","journal-title":"Int J Pediatr Otorhinolaryngol"},{"issue":"18","key":"10.1016\/j.diagmicrobio.2021.115390_bib0017","doi-asserted-by":"crossref","DOI":"10.3390\/ma12183010","article-title":"A literature review of metagenomics and culturomics of the peri-implant microbiome: Current evidence and future perspectives","volume":"12","author":"Martellacci","year":"2019","journal-title":"Materials (Basel)"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2021.115390_bib0018","doi-asserted-by":"crossref","first-page":"E371","DOI":"10.1002\/lary.26579","article-title":"Microbiomes of the normal middle ear and ears with chronic otitis media","volume":"127","author":"Minami","year":"2017","journal-title":"Laryngoscope"},{"key":"10.1016\/j.diagmicrobio.2021.115390_bib0019","doi-asserted-by":"crossref","first-page":"2538","DOI":"10.1128\/JCM.01068-16","article-title":"Molecular microbiological profile of chronic suppurative otitis media","volume":"54","author":"Neeff","year":"2016","journal-title":"J Clin Microbiol"},{"issue":"5","key":"10.1016\/j.diagmicrobio.2021.115390_bib0020","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1111\/trf.13477","article-title":"Microbiome from healthy donors assessed by 16S targeted metagenomic sequencing","volume":"56","author":"Pa\u00efss\u00e9","year":"2016","journal-title":"Transfusion"},{"issue":"12","key":"10.1016\/j.diagmicrobio.2021.115390_bib0021","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1097\/INF.0000000000002486","article-title":"Comprehensive detection of respiratory bacterial and viral pathogens in the middle ear fluid and nasopharynx of pediatric patients with acute otitis media","volume":"38","author":"Sawada","year":"2019","journal-title":"Pediatr Infect Dis J"},{"key":"10.1016\/j.diagmicrobio.2021.115390_bib0022","doi-asserted-by":"crossref","first-page":"16063","DOI":"10.1038\/nrdp.2016.63","article-title":"(2016) Otitis media","volume":"2","author":"Schilder","year":"2016","journal-title":"Nat Rev Dis Primers"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2021.115390_bib0023","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00006-17","article-title":"Next-generation sequencing combined with specific PCR assays to determine the bacterial 16S rRNA gene profiles of middle ear fluid collected from children with acute otitis media","volume":"2","author":"Sillanp\u00e4\u00e4","year":"2017","journal-title":"mSphere"},{"key":"10.1016\/j.diagmicrobio.2021.115390_bib0024","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/1471-2288-5-35","article-title":"Parametric versus non-parametric statistics in the analysis of randomized trials with non-normally distributed data","volume":"5","author":"Vickers","year":"2005","journal-title":"BMC Med Res Methodol"},{"issue":"7415","key":"10.1016\/j.diagmicrobio.2021.115390_bib0025","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1038\/nature11553","article-title":"Genomic approaches to studying the human microbiota","volume":"489","author":"Weinstock","year":"2012","journal-title":"Nature"},{"key":"10.1016\/j.diagmicrobio.2021.115390_bib0026","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1097\/MAO.0000000000002355","article-title":"Microbiome Analysis of Cholesteatoma by Gene Sequencing","volume":"40","author":"Weiss","year":"2019","journal-title":"Otol Neurotol"},{"issue":"9","key":"10.1016\/j.diagmicrobio.2021.115390_bib0027","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1097\/MAO.0000000000002355","article-title":"Microbiome analysis of cholesteatoma by gene sequencing","volume":"40","author":"Weiss","year":"2019","journal-title":"Otol Neurotol"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2021.115390_bib0028","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1097\/MAO.0b013e31819225a0","article-title":"Is the healthy middle ear a normally sterile site?","volume":"30","author":"Westerberg","year":"2009","journal-title":"Otol Neurotol"},{"key":"10.1016\/j.diagmicrobio.2021.115390_bib0029","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.3389\/fgene.2019.01176","article-title":"Comparative analysis of microbiome in nasopharynx and middle ear in young children with acute otitis media","volume":"10","author":"Xu","year":"2019","journal-title":"Front Genet"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889321000833?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889321000833?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T09:55:57Z","timestamp":1760003757000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889321000833"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["S0732889321000833"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2021.115390","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2021,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Microbiological investigation of samples collected from healthy middle ears during cochlear implant surgery","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2021.115390","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"115390"}}