{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T02:33:43Z","timestamp":1794018823930,"version":"4.8.0"},"reference-count":24,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2021,10]]},"DOI":"10.1016\/j.diagmicrobio.2021.115481","type":"journal-article","created":{"date-parts":[[2021,7,9]],"date-time":"2021-07-09T04:43:06Z","timestamp":1625805786000},"page":"115481","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"title":["Activity of \u03b2-lactam plus \u03b2-lactam-enhancer combination cefepime\/zidebactam against Klebsiella pneumoniae harbouring defective OmpK35\/36 porins and carbapenemases"],"prefix":"10.1016","volume":"101","author":[{"given":"Prashant","family":"Joshi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahul","family":"Shrivastava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachin","family":"Bhagwat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahesh","family":"Patel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0001","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1093\/jac\/dkz493","article-title":"In vitro activity of a novel antibacterial agent, levonadifloxacin, against clinical isolates collected in a prospective, multicentre surveillance study in India during 2016-18","volume":"75","author":"Appalaraju","year":"2020","journal-title":"J Antimicrob Chemother"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0002","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1089\/mdr.2018.0065","article-title":"Effect of porins and blaKPC expression on activity of imipenem with relebactam in Klebsiella pneumoniae: Can antibiotic combinations overcome resistance?","volume":"24","author":"Balabanian","year":"2018","journal-title":"Microb Drug Resist"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2021.115481_bib0003","doi-asserted-by":"crossref","DOI":"10.1016\/j.diagmicrobio.2021.115327","article-title":"Hellenic cefepime\/zidebactam study group. In vitro activity of cefepime\/zidebactam (WCK 5222) against recent Gram-negative isolates collected from high resistance settings of greek hospitals","volume":"100","author":"Bhagwat","year":"2021","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0004","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a025247","article-title":"\u03b2-lactams and \u03b2-lactamase inhibitors: an overview","volume":"6","author":"Bush","year":"2016","journal-title":"Cold Spring Harb Perspect Med"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0005","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1128\/jcm.24.3.330-332.1986","article-title":"Rapid microprocedure for isolating detergent-insoluble outer membrane proteins from Haemophilus species","volume":"24","author":"Carlone","year":"1986","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0006","series-title":"Performance Standards for Antimicrobial Susceptibility Testing. CLSI supplement M100","author":"CLSI","year":"2020"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2021.115481_bib0007","doi-asserted-by":"crossref","first-page":"3332","DOI":"10.1128\/AAC.47.10.3332-3335.2003","article-title":"Role of klebsiella pneumoniae OmpK35 porin in antimicrobial resistance","volume":"47","author":"Dom\u00e9nech-S\u00e1nchez","year":"2003","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0008","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.00537-17","article-title":"Resistance to ceftazidime-avibactam in Klebsiella pneumoniae due to porin mutations and the increased expression of KPC-3","volume":"61","author":"Humphries","year":"2017","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0009","doi-asserted-by":"crossref","first-page":"3396","DOI":"10.1128\/AAC.00285-06","article-title":"High-level carbapenem resistance in a Klebsiella pneumoniae clinical isolate is due to the combination of bla(ACT-1) beta-lactamase production, porin OmpK35\/36 insertional inactivation, and down-regulation of the phosphate transport porin PhoE","volume":"50","author":"Kaczmarek","year":"2006","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0010","doi-asserted-by":"crossref","first-page":"151","DOI":"10.3389\/fpubh.2019.00151","article-title":"The \u201cold\u201d and the \u201cnew\u201d antibiotics for MDR Gram-negative pathogens: for whom, when, and how","volume":"7","author":"Karaiskos","year":"2019","journal-title":"Front Public Health"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0011","doi-asserted-by":"crossref","DOI":"10.1093\/jac\/dkab183","article-title":"Comparative in vivo activity of human-simulated plasma and epithelial lining fluid exposures of WCK 5222 (cefepime\/zidebactam) against KPC- and OXA-48-like-producing enterobacterales in the neutropenic murine pneumonia model","author":"Lasko","year":"2021","journal-title":"J Antimicrob Chemother"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0012","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J Biol Chem"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0013","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1093\/jac\/dkaa036","article-title":"Effective inhibition of PBPs by cefepime and zidebactam in the presence of VIM-1 drives potent bactericidal activity against MBL-expressing Pseudomonas aeruginosa","volume":"75","author":"Moya","year":"2020","journal-title":"J Antimicrob Chemother"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0014","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.3201\/eid1710.110655","article-title":"Global spread of carbapenemase-producing Enterobacteriaceae","volume":"17","author":"Nordmann","year":"2011","journal-title":"Emer Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0015","series-title":"Sunday","article-title":"Cefepime (FEP) and WCK 5107 (zidebactam, ZID) mediated dual PBP engagement at sub-MIC concentrations drive cidality against diverse \u03b2-lactamases expressing Gram-negatives","volume":"448","author":"Palwe","year":"2016"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0016","doi-asserted-by":"crossref","first-page":"4943","DOI":"10.1128\/AAC.00296-11","article-title":"Carbapenems: past, present, and future","volume":"55","author":"Papp-Wallace","year":"2011","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0017","doi-asserted-by":"crossref","first-page":"4067","DOI":"10.1021\/acs.jmedchem.8b00091","article-title":"Strategic approaches to overcome resistance against Gram-Negative pathogens using \u03b2-Lactamase inhibitors and \u03b2-Lactam enhancers: activity of three novel diazabicyclooctanes WCK 5153, Zidebactam (WCK 5107), and WCK 4234","volume":"61","author":"Papp-Wallace","year":"2018","journal-title":"J Med Chem"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0018","doi-asserted-by":"crossref","first-page":"2382","DOI":"10.1128\/AAC.44.9.2382-2388.2000","article-title":"Characterization of the extended-spectrum \u03b2-lactamase reference strain, Klebsiella pneumoniae K6 (ATCC 700603), which produces the novel enzyme SHV-18","volume":"44","author":"Rasheed","year":"2000","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0019","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1128\/AAC.31.8.1164","article-title":"Leakage of \u03b2-lactamases: a second mechanism for antibiotic potentiation by amdinocillin","volume":"31","author":"Sanders","year":"1987","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0020","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/0022-2836(91)90880-F","article-title":"Carboxy-terminal phenylalanine is essential for the correct assembly of a bacterial outer membrane protein","volume":"218","author":"Struyv\u00e9","year":"1991","journal-title":"J Mol Biol"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0021","doi-asserted-by":"crossref","first-page":"3200","DOI":"10.1128\/JB.00590-16","article-title":"Klebsiella pneumoniae major porins OmpK35 and OmpK36 allow more efficient diffusion of \u03b2-lactams than their Escherichia coli homologs OmpF and OmpC","volume":"198","author":"Sugawara","year":"2016","journal-title":"J Bacteriol"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0022","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.ijid.2014.05.021","article-title":"Multi-drug carbapenem-resistant Klebsiella pneumoniae infection carrying the OXA-48 gene and showing variations in outer membrane protein 36 causing an outbreak in a tertiary care hospital in Riyadh, Saudi Arabia","volume":"28","author":"Uz Zaman","year":"2014","journal-title":"Int J Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0023","doi-asserted-by":"crossref","first-page":"3957","DOI":"10.1038\/s41467-019-11756-y","article-title":"OmpK36-mediated carbapenem resistance attenuates ST258 Klebsiella pneumoniae in vivo","volume":"10","author":"Wong","year":"2019","journal-title":"Nat Commun"},{"key":"10.1016\/j.diagmicrobio.2021.115481_bib0024","doi-asserted-by":"crossref","first-page":"S285","DOI":"10.1093\/ofid\/ofz360.679","article-title":"Meropenem-vaborbactam (MV) in vitro activity against carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates with outer membrane porin gene mutations","volume":"6","author":"Yasmin","year":"2019","journal-title":"Open Forum Infect Dis"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889321001747?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889321001747?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T09:57:01Z","timestamp":1760003821000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889321001747"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10]]},"references-count":24,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,10]]}},"alternative-id":["S0732889321001747"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2021.115481","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2021,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Activity of \u03b2-lactam plus \u03b2-lactam-enhancer combination cefepime\/zidebactam against Klebsiella pneumoniae harbouring defective OmpK35\/36 porins and carbapenemases","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2021.115481","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"115481"}}