{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:32:44Z","timestamp":1760056364907,"version":"build-2065373602"},"reference-count":24,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2022,12]]},"DOI":"10.1016\/j.diagmicrobio.2022.115801","type":"journal-article","created":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T12:32:48Z","timestamp":1661776368000},"page":"115801","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["Simultaneous detection of 9 respiratory pathogens using a newly developed multiplex real-time PCR panel based on an automatic molecular detection and analysis system"],"prefix":"10.1016","volume":"104","author":[{"given":"Meng-chuan","family":"Zhao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gui-xia","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan-qing","family":"Tie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye-huan","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-fu","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen-chao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su-xia","family":"Duan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Zhai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan-long","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di-jun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xian-ping","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-hui","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-shan","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0001","doi-asserted-by":"crossref","first-page":"3085","DOI":"10.1007\/s00705-021-05214-8","article-title":"Exploring the epidemiological changes of common respiratory viruses since the COVID-19 pandemic: a hospital study in Hangzhou, China","volume":"166","author":"Du","year":"2021","journal-title":"Arch Virol"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0002","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1186\/s12985-021-01627-8","article-title":"Impact of COVID-19 pandemic on the prevalence of respiratory viruses in children with lower respiratory tract infections in China","volume":"18","author":"Liu","year":"2021","journal-title":"Virol J"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0003","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1016\/j.jinf.2020.11.010","article-title":"SARS-CoV-2 has displaced other seasonal respiratory viruses: results from a prospective cohort study","volume":"81","author":"Poole","year":"2020","journal-title":"J Infect"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0004","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1007\/s12519-021-00477-2","article-title":"Surges of hospital-based rhinovirus infection during the 2020 coronavirus disease-19 (COVID-19) pandemic in Beijing, China","volume":"17","author":"Zhang","year":"2021","journal-title":"World J Pediatr"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0005","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1016\/S0140-6736(18)32279-7","article-title":"Global, regional, and national incidence, prevalence, and years lived with disability for 354 diseases and injuries for 195 countries and territories, 1990\u20132017: a systematic analysis for the Global Burden of Disease Study 2017","volume":"392","author":"James","year":"2018","journal-title":"Lancet"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0006","doi-asserted-by":"crossref","first-page":"1736","DOI":"10.1016\/S0140-6736(18)32203-7","article-title":"Global, regional, and national age sex-specific mortality for 282 causes of death in 195 countries and territories, 1980\u20132017: a systematic analysis for the Global Burden of disease Study 2017","volume":"392","author":"Roth","year":"2018","journal-title":"Lancet"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0007","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1093\/cid\/cix1144","article-title":"Laboratory-confirmed respiratory infections as predictors of hospital admission for myocardial infarction and stroke: time series analysis of English data for 2004-2015","volume":"67","author":"Blackburn","year":"2018","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0008","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1093\/infdis\/jis415","article-title":"Mortality attributable to 9 common infections: significant effect of influenza A, respiratory syncytial virus, influenza B, norovirus, and parainfluenza in elderly persons","volume":"206","author":"Van Asten","year":"2012","journal-title":"J Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0009","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S1473-3099(01)00170-0","article-title":"Estimates of worldwide distribution of child deaths from acute respiratory infections","volume":"2","author":"Williams","year":"2002","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0010","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1002\/jmv.24299","article-title":"Comparison of the conventional multiplex RT-PCR, real time RT-PCR and Luminex xTAG\u00ae RVP fast assay for the detection of respiratory viruses","volume":"88","author":"Choudhary","year":"2016","journal-title":"J Med Virol"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0011","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.diagmicrobio.2016.06.018","article-title":"Partial comparison of the NxTAG respiratory pathogen panel assay with the Luminex xTAG respiratory panel fast assay V2 and single plex real-time polymerase chain reaction for detection of respiratory pathogens","volume":"86","author":"Esposito","year":"2016","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0012","doi-asserted-by":"crossref","first-page":"e01945","DOI":"10.1128\/JCM.01945-17","article-title":"Multicenter evaluation of biofire filmarray respiratory panel 2 for detection of viruses and bacteria in nasopharyngeal swab samples","volume":"56","author":"Leber","year":"2018","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0013","doi-asserted-by":"crossref","DOI":"10.1016\/j.diagmicrobio.2019.114880","article-title":"Film array respiratory panel assay: An effective method for detecting viral and atypical bacterial pathogens in bronchoscopy specimens","volume":"95","author":"Locher","year":"2019","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0014","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.jcv.2010.09.022","article-title":"Evaluation of commercial ResPlex II v2.0, MultiCode-PLx, and xTAG respiratory viral panels for the diagnosis of respiratory viral infections in adults","volume":"50","author":"Balada-Llasat","year":"2011","journal-title":"J Clin Virol"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0015","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.jcv.2011.12.020","article-title":"Comparison of two broadly multiplexed PCR systems for viral detection in clinical respiratory tract specimens from immunocompromised children","volume":"53","author":"Hayden","year":"2012","journal-title":"J Clin Virol"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0016","doi-asserted-by":"crossref","first-page":"e01658","DOI":"10.1128\/JCM.01658-17","article-title":"Multicenter evaluation of the ePlex respiratory pathogen panel for the detection of viral and bacterial respiratory tract pathogens in nasopharyngeal swabs","volume":"56","author":"Babady","year":"2018","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0017","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1007\/s10096-017-3151-0","article-title":"Clinical implications of rapid ePlex\u00ae respiratory pathogen panel testing compared to laboratory-developed real-time PCR","volume":"37","author":"van Rijn","year":"2018","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0018","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1177\/2472630318787452","article-title":"Rapid detection of respiratory pathogens for community-acquired pneumonia by capillary electrophoresis-based multiplex PCR","volume":"24","author":"Li","year":"2019","journal-title":"SLAS Technol"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0019","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.diagmicrobio.2017.03.010","article-title":"Clinical evaluation of a new single-tube multiplex reverse transcription PCR assay for simultaneous detection of 11 respiratory viruses, Mycoplasma pneumoniae and Chlamydia in hospitalized children with acute respiratory infections","volume":"88","author":"Zhao","year":"2017","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0020","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1186\/s12866-021-02408-7","article-title":"Multiplex polymerase chain reaction detection of Streptococcus pneumoniae and Hemophilus influenzae and their antibiotic resistance in patients with community-acquired pneumonia from southwest Iran","volume":"21","author":"Farajzadeh Sheikh","year":"2021","journal-title":"BMC Microbiol"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0021","doi-asserted-by":"crossref","first-page":"788.e1","DOI":"10.1016\/j.cmi.2015.05.004","article-title":"Development of two real-time multiplex PCR assays for the detection and quantification of eight key bacterial pathogens in lower respiratory tract infections","volume":"21","author":"Gadsby","year":"2015","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0022","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0253402","article-title":"Development of a multiplex real-time PCR assay for the simultaneous detection of four bacterial pathogens causing pneumonia","volume":"16","author":"Lim","year":"2021","journal-title":"PLoS One"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0023","doi-asserted-by":"crossref","first-page":"2855","DOI":"10.1007\/s00705-018-3921-8","article-title":"Clinical evaluation of a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction assays for the detection of 16 respiratory viruses associated with community-acquired pneumonia","volume":"163","author":"Zhang","year":"2018","journal-title":"Arch Virol"},{"key":"10.1016\/j.diagmicrobio.2022.115801_bib0024","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jviromet.2019.04.001","article-title":"A multiplex RT-PCR assay for rapid and simultaneous detection of four RNA viruses in swine","volume":"269","author":"Zhao","year":"2019","journal-title":"J Virol Methods"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889322001675?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889322001675?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T09:57:32Z","timestamp":1760003852000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889322001675"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12]]},"references-count":24,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["S0732889322001675"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2022.115801","relation":{},"ISSN":["0732-8893"],"issn-type":[{"type":"print","value":"0732-8893"}],"subject":[],"published":{"date-parts":[[2022,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Simultaneous detection of 9 respiratory pathogens using a newly developed multiplex real-time PCR panel based on an automatic molecular detection and analysis system","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2022.115801","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"115801"}}