{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T20:17:31Z","timestamp":1776716251935,"version":"3.51.2"},"reference-count":18,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.fr","clinicalkey.jp","clinicalkey.es","clinicalkey.com.au","clinicalkey.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2024,6]]},"DOI":"10.1016\/j.diagmicrobio.2024.116265","type":"journal-article","created":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T07:57:51Z","timestamp":1710403071000},"page":"116265","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["Validation of a new extraction-free real-time PCR test to detect MPOX virus"],"prefix":"10.1016","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6301-2189","authenticated-orcid":false,"given":"Amorce","family":"Lima","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Rowe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzane","family":"Silbert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0001","doi-asserted-by":"crossref","first-page":"129","DOI":"10.2217\/fvl.12.130","article-title":"A review of experimental and natural infections of animals with monkeypox virus between 1958 and 2012","volume":"8","author":"Parker","year":"2013","journal-title":"Future Virol"},{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0002","first-page":"593","article-title":"A human infection caused by monkeypox virus in Basankusu Territory, Democratic Republic of the Congo","volume":"46","author":"Ladnyj","year":"1972","journal-title":"Bull World Health Organ"},{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0003","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1056\/NEJMoa032299","article-title":"The detection of monkeypox in humans in the Western Hemisphere","volume":"350","author":"Reed","year":"2004","journal-title":"N Engl J Med"},{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0004","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.1099\/vir.0.81215-0","article-title":"A tale of two clades: monkeypox viruses","volume":"86","author":"Likos","year":"2005","journal-title":"J Gen Virol"},{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0005","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2220415120","article-title":"Virulence differences of mpox (monkeypox) virus clades I, IIa, and IIb.1 in a small animal model","volume":"120","author":"Americo","year":"2023","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0006","doi-asserted-by":"crossref","first-page":"8","DOI":"10.3390\/tropicalmed1010008","article-title":"Human monkeypox: current state of knowledge and implications for the future","volume":"1","author":"Brown","year":"2016","journal-title":"Trop Med Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0007","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s12941-022-00527-1","article-title":"Clinical features, hospitalisation and deaths associated with monkeypox: a systematic review and meta-analysis","volume":"21","author":"Benites-Zapata","year":"2022","journal-title":"Ann Clin Microbiol Antimicrob"},{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0008","article-title":"Human monkeypox: a review of the literature","volume":"18","author":"El Eid","year":"2022","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0009","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.jviromet.2010.07.012","article-title":"Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA","volume":"169","author":"Li","year":"2010","journal-title":"J Virol Methods"},{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0010","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.jviromet.2016.07.024","article-title":"Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay","volume":"236","author":"Maksyutov","year":"2016","journal-title":"J Virol Methods"},{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0011","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.jcv.2006.03.012","article-title":"Detection of monkeypox virus with real-time PCR assays","volume":"36","author":"Li","year":"2006","journal-title":"J Clin Virol"},{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0012","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1373\/clinchem.2006.068635","article-title":"Real-time PCR to identify variola virus or other human pathogenic orthopox viruses","volume":"53","author":"Scaramozzino","year":"2007","journal-title":"Clin Chem"},{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0013","doi-asserted-by":"crossref","first-page":"e27902","DOI":"10.1002\/jmv.27902","article-title":"Re-emerging human monkeypox: A major public-health debacle","volume":"95","author":"Saxena","year":"2023","journal-title":"J Med Virol"},{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0014","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1126\/science.add4153","article-title":"Multiple lineages of monkeypox virus detected in the United States, 2021-2022","volume":"378","author":"Gigante","year":"2022","journal-title":"Science"},{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0015","doi-asserted-by":"crossref","first-page":"1940","DOI":"10.1128\/JCM.42.5.1940-1946.2004","article-title":"Real-time PCR system for detection of orthopoxviruses and simultaneous identification of smallpox virus","volume":"42","author":"Olson","year":"2004","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0016","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01604-18","article-title":"A TaqMan probe-based real-time PCR assay for the rapid identification of the emerging multidrug-resistant pathogen Candida auris on the BD max system","volume":"57","author":"Lima","year":"2019","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0017","doi-asserted-by":"crossref","first-page":"3935","DOI":"10.1128\/JCM.01962-15","article-title":"Evaluation of the new BD max GC real-time PCR assay, analytically and clinically as a supplementary test for the BD ProbeTec GC Qx amplified DNA assay, for molecular detection of Neisseria gonorrhoeae","volume":"53","author":"Golparian","year":"2015","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2024.116265_bib0018","doi-asserted-by":"crossref","first-page":"405","DOI":"10.4269\/ajtmh.16-0567","article-title":"Evaluation of the GeneXpert for human monkeypox diagnosis","volume":"96","author":"Li","year":"2017","journal-title":"Am J Trop Med Hyg"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889324000944?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889324000944?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,4,27]],"date-time":"2024-04-27T09:07:34Z","timestamp":1714208854000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889324000944"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["S0732889324000944"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2024.116265","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2024,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Validation of a new extraction-free real-time PCR test to detect MPOX virus","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2024.116265","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"116265"}}