{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T03:46:02Z","timestamp":1776224762850,"version":"3.50.1"},"reference-count":34,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100005703","name":"Akdeniz University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005703","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.fr","clinicalkey.jp","clinicalkey.es","clinicalkey.com.au","clinicalkey.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2024,12]]},"DOI":"10.1016\/j.diagmicrobio.2024.116547","type":"journal-article","created":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T17:11:55Z","timestamp":1727370715000},"page":"116547","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"title":["A rapid and simple modified nitrate reductase assay for testing first and second-line antituberculosis drug susceptibilities in Mycobacterium tuberculosis isolates"],"prefix":"10.1016","volume":"110","author":[{"given":"Ece","family":"Simsek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kubra","family":"Yildirim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cemilenur","family":"Atas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmet Yilmaz","family":"Coban","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.diagmicrobio.2024.116547_bib0001","series-title":"Global tuberculosis report 2023","year":"2023"},{"issue":"9","key":"10.1016\/j.diagmicrobio.2024.116547_bib0002","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a017863","article-title":"Multidrug-Resistant tuberculosis and extensively drug-resistant tuberculosis","volume":"5","author":"Seung","year":"2015","journal-title":"Cold Spring Harb Perspect Med"},{"key":"10.1016\/j.diagmicrobio.2024.116547_bib0003","series-title":"Who target product profile for next-generation drug-susceptibility testing at peripheral centers","year":"2021"},{"key":"10.1016\/j.diagmicrobio.2024.116547_bib0004","series-title":"Technical manual for drug susceptibility testing of medicines used in the treatment of tuberculosis","year":"2018"},{"issue":"5","key":"10.1016\/j.diagmicrobio.2024.116547_bib0005","first-page":"565","article-title":"Laboratory methods for testing drug sensitivity and resistance","volume":"29","author":"Canetti","year":"1963","journal-title":"Bull World Health Organ"},{"key":"10.1016\/j.diagmicrobio.2024.116547_bib0006","unstructured":"Clinical and Laboratory Standards Institute (2011). Susceptibility testing of mycobacteria, nocardia spp., and other aerobic actinomycetes. 2nd edition., Available from https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK544374 PMID: 31339680."},{"issue":"2","key":"10.1016\/j.diagmicrobio.2024.116547_bib0007","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1128\/CMR.00059-10","article-title":"Laboratory diagnosis of tuberculosis in resource-poor countries: challenges and opportunities","volume":"24","author":"Parsons","year":"2011","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.diagmicrobio.2024.116547_bib0008","unstructured":"Palomino JC, Leao SL, Ritacco V. Tuberculosis 2007 From basic science to patient care. 1th ed. 2007. Amedeo Challenge."},{"issue":"8","key":"10.1016\/j.diagmicrobio.2024.116547_bib0009","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1111\/j.1469-0691.2007.01698.x","article-title":"Rapid drug resistance detection in Mycobacterium tuberculosis: a review of colourimetric methods","volume":"13","author":"Palomino","year":"2007","journal-title":"Clin Microbiol Infect"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2024.116547_bib0010","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1016\/j.ccm.2009.08.016","article-title":"Novel and improved technologies for tuberculosis diagnosis: progress and challenges","volume":"30","author":"Pai","year":"2009","journal-title":"Clin Chest Med"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2024.116547_bib0011","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1093\/jac\/dkl477","article-title":"Colorimetric redox-indicator methods for the rapid detection of multidrug resistance in Mycobacterium tuberculosis: a systematic review and meta-analysis","volume":"59","author":"Martin","year":"2007","journal-title":"J Antimicrob Chemother"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2024.116547_bib0012","doi-asserted-by":"crossref","first-page":"19","DOI":"10.4103\/ijmy.ijmy_194_20","article-title":"Validation of a novel medium for drug susceptibility testing of mycobacterium tuberculosis against first- and second-line drugs: AYC.2.2 agar and AYC.2.1 broth","volume":"10","author":"Coban","year":"2021","journal-title":"Int J Mycobacteriol"},{"key":"10.1016\/j.diagmicrobio.2024.116547_bib0013","series-title":"Public health mycobacteriology. A guide for the level III laboratory. U.S","author":"Kent","year":"1985"},{"key":"10.1016\/j.diagmicrobio.2024.116547_bib0014","series-title":"Laboratory serv\u0131ces in tuberculos\u0131s control culture part III","year":"1998"},{"key":"10.1016\/j.diagmicrobio.2024.116547_bib0015","unstructured":"Food and Drug Administration (FDA). (2007). Guidance for industry and FDA Staff- Statistical Guidance on reporting results from studies evaluating diagnostic tests. USA."},{"issue":"7","key":"10.1016\/j.diagmicrobio.2024.116547_bib0016","doi-asserted-by":"crossref","first-page":"3159","DOI":"10.1128\/JCM.43.7.3159-3161.2005","article-title":"Drug susceptibility testing of Mycobacterium tuberculosis by a nitrate reductase assay applied directly on microscopy-positive sputum samples","volume":"43","author":"Musa","year":"2005","journal-title":"J Clin Microbiol"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2024.116547_bib0017","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1093\/jac\/dkt353","article-title":"Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis","volume":"69","author":"Martin","year":"2014","journal-title":"J Antimicrob Chemother"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2024.116547_bib0018","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.ijantimicag.2004.02.027","article-title":"Drug susceptibility testing of Mycobacterium tuberculosis with nitrate reductase assay","volume":"24","author":"Coban","year":"2004","journal-title":"Int J Antimicrob Agents"},{"issue":"Pt 7","key":"10.1016\/j.diagmicrobio.2024.116547_bib0019","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1099\/jmm.0.46540-0","article-title":"Nitrate reductase assay for detection of drug resistance in Mycobacterium tuberculosis: simple and inexpensive method for low-resource laboratories","volume":"55","author":"Lemus","year":"2006","journal-title":"J Med Microbiol"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2024.116547_bib0020","doi-asserted-by":"crossref","first-page":"363","DOI":"10.4103\/0255-0857.71816","article-title":"Evaluation of nitrate reductase assay for direct detection of drug resistance in Mycobacterium tuberculosis: rapid and inexpensive method for low-resource settings","volume":"28","author":"Gupta","year":"2010","journal-title":"Indian J Med Microbiol"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2024.116547_bib0021","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.mimet.2011.11.006","article-title":"Evaluation of nitrate reduction assay, resazurin microtiter assay and microscopic observation drug susceptibility assay for first line antitubercular drug susceptibility testing of clinical isolates of M. tuberculosis","volume":"88","author":"Dixit","year":"2012","journal-title":"J Microbiol Methods"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2024.116547_bib0022","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.mimet.2005.03.004","article-title":"Multicenter evaluation of the nitrate reductase assay for drug resistance detection of Mycobacterium tuberculosis","volume":"63","author":"Martin","year":"2005","journal-title":"J Microbiol Methods"},{"key":"10.1016\/j.diagmicrobio.2024.116547_bib0023","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mimet.2017.07.001","article-title":"Systematic review and meta-analysis of the nitrate reductase assay for drug susceptibility testing of Mycobacterium tuberculosis and the detection limits in liquid medium","volume":"141","author":"Kwak","year":"2017","journal-title":"J Microbiol Methods"},{"key":"10.1016\/j.diagmicrobio.2024.116547_bib0024","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s41182-018-0109-6","article-title":"Evaluation of nitrate reductase assay in 7H11 agar for diagnosis of multi-drug-resistant tuberculosis in eastern Nepal","volume":"46","author":"Halwai","year":"2018","journal-title":"Trop Med Health"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2024.116547_bib0025","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1590\/S0074-02762011000300022","article-title":"A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar","volume":"106","author":"Coban","year":"2011","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2024.116547_bib0026","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.mimet.2010.03.005","article-title":"Simple and rapid method for detection of nitrate reductase activity of Mycobacterium tuberculosis and Mycobacterium canettii grown in the Bactec MGIT960 system","volume":"81","author":"Goh","year":"2010","journal-title":"J Microbiol Methods"},{"issue":"7","key":"10.1016\/j.diagmicrobio.2024.116547_bib0027","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1007\/s10096-003-0932-4","article-title":"Rapid testing for nitrate reductase activity of Mycobacterium tuberculosis grown in an automated culture system","volume":"22","author":"Meyer","year":"2003","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2024.116547_bib0028","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1186\/1471-2334-12-326","article-title":"The manual mycobacteria growth indicator tube and the nitrate reductase assay for the rapid detection of rifampicin resistance of M. Tuberculosis in low resource settings","volume":"12","author":"Adikaram","year":"2012","journal-title":"BMC Infect Dis"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2024.116547_bib0029","doi-asserted-by":"crossref","first-page":"3243","DOI":"10.1128\/JCM.00083-08","article-title":"Rapid and inexpensive detection of multidrug-resistant Mycobacterium tuberculosis with the nitrate reductase assay using liquid medium and direct application to sputum samples","volume":"46","author":"Affolabi","year":"2008","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2024.116547_bib0030","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.tube.2018.11.001","article-title":"Multicentre laboratory validation of the nitrate reductase assay using liquid medium for the rapid detection of multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis","volume":"113","author":"Huang","year":"2018","journal-title":"Tuberculosis (Edinb)"},{"issue":"11","key":"10.1016\/j.diagmicrobio.2024.116547_bib0031","doi-asserted-by":"crossref","first-page":"5173","DOI":"10.1128\/JCM.41.11.5173-5177.2003","article-title":"Rapid colorimetric method for testing susceptibility of Mycobacterium tuberculosis to isoniazid and rifampin in liquid cultures","volume":"41","author":"Syre","year":"2003","journal-title":"J Clin Microbiol"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2024.116547_bib0032","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/S0255-0857(21)02287-8","article-title":"Rapid antibiotic susceptibility testing of Mycobacterium tuberculosis: its utility in resource poor settings","volume":"24","author":"Poojary","year":"2006","journal-title":"Indian J Med Microbiol"},{"key":"10.1016\/j.diagmicrobio.2024.116547_bib0033","unstructured":"World Health Organization (2021). WHO announces updated definitions of extensively drug-resistant tuberculosis. https:\/\/www.who.int\/news\/item\/27-01-2021-who-announces-updated-definitions-of-extensively-drug-resistant-tuberculosis. (Accessed 3 December 2023)."},{"key":"10.1016\/j.diagmicrobio.2024.116547_bib0034","series-title":"Noncommercial culture and drug-susceptibility testing methods for screening patients at risk for multidrug-resistant tuberculosis: policy statement","year":"2011"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889324003729?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889324003729?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,2,23]],"date-time":"2025-02-23T04:46:06Z","timestamp":1740285966000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889324003729"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":34,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["S0732889324003729"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2024.116547","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A rapid and simple modified nitrate reductase assay for testing first and second-line antituberculosis drug susceptibilities in Mycobacterium tuberculosis isolates","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2024.116547","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"116547"}}