{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T02:53:54Z","timestamp":1778900034018,"version":"3.51.4"},"reference-count":29,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP23fk0108683"],"award-info":[{"award-number":["JP23fk0108683"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2024,12]]},"DOI":"10.1016\/j.diagmicrobio.2024.116548","type":"journal-article","created":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T02:12:31Z","timestamp":1727921551000},"page":"116548","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["A rapid economical multiplex PCR-RFLP method for molecular detection and genotyping of Giardia duodenalis clinical isolates"],"prefix":"10.1016","volume":"110","author":[{"given":"Ajanta","family":"Ghosal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjib K.","family":"Sardar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tapas","family":"Haldar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akash","family":"Prasad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koushik","family":"Das","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiki","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumiko","family":"Saito-Nakano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanta","family":"Dutta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoyoshi","family":"Nozaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4859-9759","authenticated-orcid":false,"given":"Sandipan","family":"Ganguly","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.diagmicrobio.2024.116548_bib0001","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1186\/1756-3305-5-168","article-title":"Population-based analyses of Giardia duodenalis is consistent with the clonal assemblage structure","volume":"10","author":"Takumi","year":"2012","journal-title":"Parasit Vectors"},{"issue":"116","key":"10.1016\/j.diagmicrobio.2024.116548_bib0002","article-title":"Giardiasis in urban and rural Amazonas, Brazil is driven by zoonotic and cosmopolitan A and B assemblages","volume":"18","author":"Dos Reis","year":"2022","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.diagmicrobio.2024.116548_bib0003","doi-asserted-by":"crossref","unstructured":"Zajaczkowski P, Lee R, Fletcher-Lartey SM, Alexander K, Mahimbo A, Stark D, et al. The controversies surrounding Giardia intestinalis assemblages A and B. Curr Res Parasitol Vector Borne Dis. 2021;19:1:100055. doi: 10.1016\/j.crpvbd.2021.100055. PMID: 35284870; PMCID: PMC8906113.","DOI":"10.1016\/j.crpvbd.2021.100055"},{"key":"10.1016\/j.diagmicrobio.2024.116548_bib0004","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.mib.2016.07.019","article-title":"An up-date on Giardia and giardiasis","volume":"34","author":"Einarsson","year":"2016","journal-title":"Curr Opin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2024.116548_bib0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.exppara.2023.108602","article-title":"Functional characterization of phospholipase B enzyme from Giardia lamblia","volume":"253","author":"Sarkar","year":"2023","journal-title":"Exp Parasitol"},{"issue":"11","key":"10.1016\/j.diagmicrobio.2024.116548_bib0006","article-title":"Giardiasis as a neglected disease in Brazil: Systematic review of 20 years of publications","volume":"24","author":"Coelho","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"10.1016\/j.diagmicrobio.2024.116548_bib0007","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0065-308X(04)58002-8","article-title":"Variation in Giardia: implications for taxonomy and epidemiology","volume":"58","author":"Thompson","year":"2004","journal-title":"Adv Parasitol"},{"issue":"12","key":"10.1016\/j.diagmicrobio.2024.116548_bib0008","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1093\/gbe\/evt197","article-title":"Genome sequencing of Giardia lamblia genotypes A2 and B isolates (DH and GS) and comparative analysis with the genomes of genotypes A1 and E (WB and Pig)","volume":"5","author":"Adam","year":"2013","journal-title":"Genome Biol Evol"},{"key":"10.1016\/j.diagmicrobio.2024.116548_bib0009","series-title":"Advances in medico-veterinary parasitology: an indian perspective","first-page":"22","article-title":"Giardia, giardiasis and its zoonotic transmission","author":"Raj","year":"2018"},{"key":"10.1016\/j.diagmicrobio.2024.116548_bib0010","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1016\/j.ijpara.2008.04.008","article-title":"Multilocus genotyping of Giardia duodenalis reveals striking differences between assemblages A and B","volume":"38","author":"Cacci\u00f2","year":"2008","journal-title":"Int J Parasitol"},{"key":"10.1016\/j.diagmicrobio.2024.116548_bib0011","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.actatropica.2018.06.008","article-title":"A modified PCR-RFLP method to determine genetic diversity of Giardia lamblia human isolates based on triosephosphate isomerase (TPI) gene","volume":"186","author":"Rahimian","year":"2018","journal-title":"Acta Trop"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2024.116548_bib0012","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s00436-022-07706-1","article-title":"Development of a simple PCR-RFLP technique for detection and differentiation of E. histolytica, E. dispar and E. moshkovskii","volume":"122","author":"Sardar","year":"2023","journal-title":"Parasitol Res"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2024.116548_bib0013","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/0035-9203(83)90120-7","article-title":"Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile","volume":"77","author":"Keister","year":"1983","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"10.1016\/j.diagmicrobio.2024.116548_bib0014","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1136\/jcp.23.6.545","article-title":"Further observations on the formol-ether concentration technique for faecal parasites","volume":"23","author":"Allen","year":"1970","journal-title":"J Clin Pathol"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2024.116548_bib0015","doi-asserted-by":"crossref","first-page":"445","DOI":"10.3343\/alm.2015.35.4.445","article-title":"Evaluation of modified formalinether concentration method using para tube in clinical settings","volume":"35","author":"Won","year":"2015","journal-title":"Ann Lab Med"},{"key":"10.1016\/j.diagmicrobio.2024.116548_bib0016","doi-asserted-by":"crossref","DOI":"10.1590\/0074-02760230043","article-title":"Giardia lamblia-infected preschoolers present growth delays independent of the assemblage A, B or E","volume":"118","author":"Fantinatti","year":"2023","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.diagmicrobio.2024.116548_bib0017","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1051\/parasite\/2016013","article-title":"Giardia duodenalis genetic assemblages and hosts","volume":"23","author":"Heyworth","year":"2016","journal-title":"Parasite"},{"issue":"11","key":"10.1016\/j.diagmicrobio.2024.116548_bib0018","doi-asserted-by":"crossref","first-page":"2567","DOI":"10.1007\/s00436-023-07956-7","article-title":"Genotyping and epidemiological distribution of diarrhea-causing isolates of Giardia duodenalis in southeastern part of West Bengal","volume":"122","author":"Ghosal","year":"2023","journal-title":"India. Parasitol Res."},{"key":"10.1016\/j.diagmicrobio.2024.116548_bib0019","unstructured":"https:\/\/www.idtdna.com\/pages\/education\/decoded\/article\/calculations-converting-from-nanograms-to-copy-number."},{"key":"10.1016\/j.diagmicrobio.2024.116548_bib0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.meegid.2023.105482","article-title":"Genetic diversity and molecular diagnosis of Giardia","volume":"113","author":"Chang","year":"2023","journal-title":"Infect Genet Evol"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2024.116548_bib0021","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1002\/elps.200900480","article-title":"Analysis of nucleotide variation within the triose-phosphate isomerase gene of Giardia duodenalis from sheep and its zoonotic implications","volume":"31","author":"Nolan","year":"2010","journal-title":"Electrophoresis"},{"key":"10.1016\/j.diagmicrobio.2024.116548_bib0022","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.fawpar.2017.09.002","article-title":"Molecular epidemiologic tools for waterborne pathogens Cryptosporidium spp. and Giardia duodenalis","volume":"8-9","author":"Xiao","year":"2017","journal-title":"Food Waterborne Parasitol"},{"issue":"11","key":"10.1016\/j.diagmicrobio.2024.116548_bib0023","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.3201\/eid0911.030084","article-title":"Triosephosphate isomerase gene characterization and potential zoonotic transmission of Giardia duodenalis","volume":"9","author":"Sulaiman","year":"2003","journal-title":"Emerg Infect Dis"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2024.116548_bib0024","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1645\/GE-1670.1","article-title":"Real-time PCR\/RFLP assay to detect Giardia intestinalis genotypes in human isolates with diarrhea in Egypt","volume":"95","author":"Helmy","year":"2009","journal-title":"J Parasitol"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2024.116548_bib0025","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1590\/0074-0276140507","article-title":"Intestinal parasites and genotyping of Giardia duodenalis in children: first report of genotype B in isolates from human clinical samples in Mexico","volume":"109","author":"Torres-Romero","year":"2014","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.diagmicrobio.2024.116548_bib0026","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1128\/JCM.40.2.446-452.2002","article-title":"Sensitive PCR-restriction fragment length polymorphism assay for detection and genotyping of Giardia duodenalis in human feces","volume":"40","author":"Amar","year":"2002","journal-title":"J Clin Microbiol"},{"issue":"Pt 8","key":"10.1016\/j.diagmicrobio.2024.116548_bib0027","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1099\/jmm.0.05193-0","article-title":"Detection and genotyping by real-time PCR\/RFLP analyses of Giardia duodenalis from human faeces","volume":"52","author":"Amar","year":"2003","journal-title":"J Med Microbiol"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2024.116548_bib0028","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3347\/kjp.2016.54.1.1","article-title":"An Improved PCR-RFLP Assay for Detection and Genotyping of Asymptomatic Giardia lamblia Infection in a Resource-Poor Setting","volume":"54","author":"Hawash","year":"2016","journal-title":"Korean J Parasitol"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2024.116548_bib0029","doi-asserted-by":"crossref","first-page":"190","DOI":"10.9734\/BMRJ\/2013\/2812","article-title":"Multilocus genotyping reveals high occurrence of mixed assemblages in Giardia duodenalis within a limited geographic boundary","volume":"3","author":"Mukherjee","year":"2013","journal-title":"Bri Microbiol Res J"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889324003730?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889324003730?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T23:27:36Z","timestamp":1778542056000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889324003730"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["S0732889324003730"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2024.116548","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A rapid economical multiplex PCR-RFLP method for molecular detection and genotyping of Giardia duodenalis clinical isolates","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2024.116548","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"116548"}}