{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T16:03:36Z","timestamp":1784563416708,"version":"3.55.0"},"reference-count":41,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2025,7]]},"DOI":"10.1016\/j.diagmicrobio.2025.116835","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T13:23:40Z","timestamp":1744118620000},"page":"116835","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development and evaluation of ELISA serological immunoassays for influenza and respiratory syncytial viruses"],"prefix":"10.1016","volume":"112","author":[{"given":"Alexandra","family":"Claudet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"No\u00e9mi","family":"Alcover","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elise","family":"Lebigot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hana-Sofia","family":"Bouhelal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fairly","family":"Warnakulasuriya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Pierre","family":"Soutiere","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Galloux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Fran\u00e7ois","family":"Eleouet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Anne","family":"Rameix-Welti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etienne","family":"Bizot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Vauloup-Fellous","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7929-0392","authenticated-orcid":false,"given":"Vincent","family":"Portet-Sulla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"10340","key":"10.1016\/j.diagmicrobio.2025.116835_bib0001","first-page":"2047\u201164","article-title":"Global, regional, and national disease burden estimates of acute lower respiratory infections due to respiratory syncytial virus in children younger than 5 years in 2019: a systematic analysis","volume":"399","author":"Li","year":"2022","journal-title":"Lancet. Mai"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2025.116835_bib0002","first-page":"93\u2011101","article-title":"Seroprevalence of influenza A and B in German infants and adolescents","volume":"198","author":"Sauerbrei","year":"2009","journal-title":"Med. Microbiol. Immunol. mai"},{"key":"10.1016\/j.diagmicrobio.2025.116835_bib0003","first-page":"793","article-title":"Trait\u00e9 de Virologie m\u00e9dicale - deuxi\u00e8me \u00e9dition","author":"Mourez","year":"2019","journal-title":"Deuxi\u00e8me \u00c9dition"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.116835_bib0004","first-page":"45\u201153","article-title":"Influenza and Influenza vaccine: a review","volume":"66","author":"Nypaver","year":"2021","journal-title":"J Midwif Women's Health. Janv"},{"key":"10.1016\/j.diagmicrobio.2025.116835_bib0005","article-title":"The recent landscape of RSV vaccine research","volume":"13","author":"Kelleher","year":"2025","journal-title":"Ther Adv Vaccines Immunother"},{"issue":"8","key":"10.1016\/j.diagmicrobio.2025.116835_bib0006","first-page":"1725\u201142","article-title":"Differences between RSV A and RSV B subgroups and implications for pharmaceutical preventive measures","volume":"13","author":"Nuttens","year":"2024","journal-title":"Infect Dis Ther. ao\u00fbt"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.116835_bib0007","article-title":"Level of maternal antibodies against respiratory syncytial virus (RSV) nucleoprotein at birth and risk of RSV very severe lower respiratory tract infection. Influenza other respir viruses","volume":"17","author":"Receveur","year":"2023","journal-title":"janv"},{"issue":"16","key":"10.1016\/j.diagmicrobio.2025.116835_bib0008","first-page":"8375\u201187","article-title":"Characterization of a viral phosphoprotein binding site on the surface of the Respiratory syncytial nucleoprotein","volume":"86","author":"Galloux","year":"2012","journal-title":"J Virol. 15 ao\u00fbt"},{"issue":"10098","key":"10.1016\/j.diagmicrobio.2025.116835_bib0009","first-page":"946\u201158","article-title":"Global, regional, and national disease burden estimates of acute lower respiratory infections due to respiratory syncytial virus in young children in 2015: a systematic review and modelling study","volume":"390","author":"Shi","year":"2017","journal-title":"Lancet. 2 sept"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.116835_bib0010","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1186\/s12879-021-06399-8","article-title":"Economic and disease burden of RSV-associated hospitalizations in young children in France, from 2010 through 2018","volume":"21","author":"Demont","year":"2021","journal-title":"BMC Infect Dis. 2 ao\u00fbt"},{"issue":"10340","key":"10.1016\/j.diagmicrobio.2025.116835_bib0011","first-page":"2047\u201164","article-title":"Global, regional, and national disease burden estimates of acute lower respiratory infections due to respiratory syncytial virus in children younger than 5 years in 2019: a systematic analysis","volume":"399","author":"Li","year":"2022","journal-title":"Lancet North Am Ed"},{"key":"10.1016\/j.diagmicrobio.2025.116835_bib0012","unstructured":"Sant\u00e9 PubliqueFrance. Sant\u00e9 Publique France - Actualit\u00e9s 2023 Bronchiolite \u00e0 VRS [Internet]. 2022 avr. Disponible sur: https:\/\/www.santepubliquefrance.fr\/maladies-et-traumatismes\/maladies-et-infections-respiratoires\/bronchiolite."},{"issue":"Supplement_7","key":"10.1016\/j.diagmicrobio.2025.116835_bib0014","doi-asserted-by":"crossref","first-page":"S577\u201183","DOI":"10.1093\/infdis\/jiz059","article-title":"Global disease burden estimates of Respiratory syncytial virus\u2013Associated acute Respiratory infection in older adults in 2015: a systematic review and meta-analysis","volume":"222","author":"Shi","year":"2020","journal-title":"J Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2025.116835_bib0015","unstructured":"Sant\u00e9 PubliqueFrance. Sant\u00e9 Publique France - Actualit\u00e9s 2023 Grippe [Internet]. 2023 mai. Disponible sur: https:\/\/www.santepubliquefrance.fr\/maladies-et-traumatismes\/maladies-et-infections-respiratoires\/grippe."},{"key":"10.1016\/j.diagmicrobio.2025.116835_bib0016","unstructured":"INSERM - DREES. Enqu\u00eate nationale p\u00e9rinatale - Rapport 2016 [Internet]. 2017 oct. Disponible sur: https:\/\/www.epop\u00e9-inserm.fr\/wp-content\/uploads\/2017\/10\/ENP2016_rapport_complet.pdf."},{"issue":"7","key":"10.1016\/j.diagmicrobio.2025.116835_bib0017","first-page":"595\u2011608","article-title":"Respiratory syncytial virus prefusion F protein vaccine in older adults","volume":"388","author":"Papi","year":"2023","journal-title":"N Engl J Med. 16 f\u00e9vr"},{"issue":"16","key":"10.1016\/j.diagmicrobio.2025.116835_bib0018","first-page":"1451\u201164","article-title":"Bivalent prefusion F vaccine in pregnancy to prevent RSV illness in infants","volume":"388","author":"Kampmann","year":"2023","journal-title":"N Engl J Med. 20 avr"},{"issue":"9","key":"10.1016\/j.diagmicrobio.2025.116835_bib0019","first-page":"837\u201146","article-title":"Nirsevimab for prevention of RSV in healthy late-preterm and term infants","volume":"386","author":"Hammitt","year":"2022","journal-title":"N Engl J Med. 3 mars"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2025.116835_bib0020","doi-asserted-by":"crossref","first-page":"96","DOI":"10.3390\/v8040096","article-title":"Current approaches for diagnosis of influenza virus infections in humans","volume":"8","author":"Vemula","year":"2016","journal-title":"Viruses. 12 avr"},{"issue":"33","key":"10.1016\/j.diagmicrobio.2025.116835_bib0021","first-page":"4591\u20117","article-title":"RSV neutralization assays - use in immune response assessment","volume":"39","author":"Raghunandan","year":"2021","journal-title":"Vaccine. 30 juill"},{"key":"10.1016\/j.diagmicrobio.2025.116835_bib0022","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.02138-24","article-title":"Detection of antibodies against influenza A viruses in cattle","author":"Lang","year":"2025","journal-title":"J Virol"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.116835_bib0023","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/s41541-024-01057-x","article-title":"Antibody responses against influenza A decline with successive years of annual influenza vaccination","volume":"10","author":"Sullivan","year":"2025","journal-title":"NPJ Vaccines. 17 janv"},{"key":"10.1016\/j.diagmicrobio.2025.116835_bib0024","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.jcv.2017.08.017","article-title":"Transplacental transfer of maternal respiratory syncytial virus (RSV) antibody and protection against RSV disease in infants in rural Nepal","volume":"95","author":"Chu","year":"2017","journal-title":"J Clin Virol"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2025.116835_bib0025","first-page":"669\u201183","article-title":"Serologic assays for influenza surveillance, diagnosis and vaccine evaluation","volume":"9","author":"Katz","year":"2011","journal-title":"Expert Review of Anti-infective Therapy. 1 juin"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2025.116835_bib0026","doi-asserted-by":"crossref","first-page":"675\u201186","DOI":"10.1111\/irv.12591","article-title":"Comparison of hemagglutination inhibition, single radial hemolysis, virus neutralization assays, and ELISA to detect antibody levels against seasonal influenza viruses","volume":"12","author":"Trombetta","year":"2018","journal-title":"Influenza Other Respi Viruses"},{"key":"10.1016\/j.diagmicrobio.2025.116835_bib0027","doi-asserted-by":"crossref","DOI":"10.1128\/CVI.00225-17","article-title":"Development of a High-Throughput Respiratory Syncytial Virus Fluorescent Focus-Based Microneutralization Assay","volume":"24","author":"Shambaugh","year":"2017","journal-title":"Clin Vaccine Immunol"},{"issue":"6158","key":"10.1016\/j.diagmicrobio.2025.116835_bib0028","first-page":"592\u20118","article-title":"Structure-based design of a fusion glycoprotein vaccine for respiratory syncytial virus","volume":"342","author":"McLellan","year":"2013","journal-title":"Science. 1"},{"issue":"693","key":"10.1016\/j.diagmicrobio.2025.116835_bib0029","doi-asserted-by":"crossref","first-page":"eade6422","DOI":"10.1126\/scitranslmed.ade6422","article-title":"Rational design of a highly immunogenic prefusion-stabilized F glycoprotein antigen for a respiratory syncytial virus vaccine","volume":"15","author":"Che","year":"2023","journal-title":"Sci Transl Med. 26 avr"},{"key":"10.1016\/j.diagmicrobio.2025.116835_bib0030","series-title":"D\u00e9termination de la taille d\u2019 un \u00e9chantillon dans les \u00e9tudes sanom\u00e9triques: manuel pratique","first-page":"84","author":"Lwanga","year":"1991"},{"issue":"5","key":"10.1016\/j.diagmicrobio.2025.116835_bib0031","doi-asserted-by":"crossref","first-page":"485\u201192","DOI":"10.1016\/j.aller.2013.03.008","article-title":"Sample size calculation","volume":"42","author":"Rodr\u00edguez Del \u00c1guila","year":"2014","journal-title":"Allergol Immunopathol (Madr)"},{"key":"10.1016\/j.diagmicrobio.2025.116835_bib0032","first-page":"177\u201183","article-title":"High seroprevalence of antibodies against human respiratory syncytial virus and evidence of respiratory syncytial virus reinfection in young children in Thailand","volume":"125","author":"Pasittungkul","year":"2022","journal-title":"Int J Infect Diseases d\u00e9c"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2025.116835_bib0033","doi-asserted-by":"crossref","first-page":"761\u201172","DOI":"10.1093\/infdis\/jiac327","article-title":"Safety and immunogenicity of a Respiratory syncytial virus prefusion F (RSVPreF3) candidate vaccine in older adults: phase 1\/2 randomized clinical trial","volume":"227","author":"Leroux-Roels","year":"2023","journal-title":"J Infect Dis"},{"issue":"Pt 1","key":"10.1016\/j.diagmicrobio.2025.116835_bib0034","first-page":"196\u2011206","article-title":"The nine C-terminal amino acids of the respiratory syncytial virus protein P are necessary and sufficient for binding to ribonucleoprotein complexes in which six ribonucleotides are contacted per N protein protomer","volume":"88","author":"Tran","year":"2007","journal-title":"J Gen Virol. janv"},{"issue":"11","key":"10.1016\/j.diagmicrobio.2025.116835_bib0035","article-title":"Respiratory syncytial virus fusion glycoprotein expressed in insect cells form protein nanoparticles that induce protective immunity in cotton rats","volume":"7","author":"Smith","year":"2012","journal-title":"Varga SM"},{"issue":"11","key":"10.1016\/j.diagmicrobio.2025.116835_bib0036","first-page":"e893\u2011902","article-title":"Transplacental transfer efficiency of maternal antibodies against influenza A(H1N1)pdm09 virus and dynamics of naturally acquired antibodies in Chinese children: a longitudinal, paired mother\u2013neonate cohort study","volume":"4","author":"Li","year":"2023","journal-title":"Lancet Microbe. 1"},{"key":"10.1016\/j.diagmicrobio.2025.116835_bib0037","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1186\/s13104-017-2720-8","article-title":"Seroprevalence of influenza A and B viruses among unvaccinated children in the United Arab Emirates: a cross-sectional study","volume":"10","author":"Alsuwaidi","year":"2017","journal-title":"BMC Res Notes. 10 ao\u00fbt"},{"issue":"33","key":"10.1016\/j.diagmicrobio.2025.116835_bib0038","first-page":"4591\u20117","article-title":"RSV neutralization assays \u2013 Use in immune response assessment","volume":"39","author":"Raghunandan","year":"2021","journal-title":"Vaccine. juill"},{"issue":"50","key":"10.1016\/j.diagmicrobio.2025.116835_bib0039","doi-asserted-by":"crossref","first-page":"7641","DOI":"10.1016\/j.vaccine.2018.10.087","article-title":"Establishment of the first WHO International Standard for antiserum to Respiratory Syncytial Virus: Report of an international collaborative study","volume":"36","author":"McDonald","year":"2018","journal-title":"Vaccine"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2025.116835_bib0040","doi-asserted-by":"crossref","first-page":"e1766","DOI":"10.1371\/journal.pone.0001766","article-title":"Sub-nucleocapsid nanoparticles: a nasal vaccine against Respiratory syncytial virus","volume":"3","author":"Roux","year":"2008","journal-title":"PLoS One. 12 mars"},{"key":"10.1016\/j.diagmicrobio.2025.116835_bib0041","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1126\/science.1243283","article-title":"Structure-based design of a fusion glycoprotein vaccine for respiratory syncytial virus","volume":"342","author":"McLellan","year":"2013","journal-title":"Science"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2025.116835_bib0042","article-title":"Site-specific serology unveils cross-reactive monoclonal antibodies targeting influenza A hemagglutinin epitopes","volume":"54","author":"Paparoditis","year":"2024","journal-title":"Eur J Immunol. oct"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889325001580?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889325001580?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T09:53:21Z","timestamp":1752573201000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889325001580"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":41,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["S0732889325001580"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2025.116835","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and evaluation of ELISA serological immunoassays for influenza and respiratory syncytial viruses","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2025.116835","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"116835"}}