{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T22:54:02Z","timestamp":1780959242882,"version":"3.54.1"},"reference-count":28,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T00:00:00Z","timestamp":1752105600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.diagmicrobio.2025.117002","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T15:08:53Z","timestamp":1752592133000},"page":"117002","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Targeted next-generation sequencing for pathogen detection and epidemiological analysis of pediatric acute respiratory infections in Xiantao, China"],"prefix":"10.1016","volume":"113","author":[{"given":"Meng-Yang","family":"Cai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1501-7919","authenticated-orcid":false,"given":"Hua-Wei","family":"Yi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Qian","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui-Min","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Hao","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Qiong","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Rong","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang-Qin","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian-Mo","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"12","key":"10.1016\/j.diagmicrobio.2025.117002_bib0001","article-title":"Clinical features and viral etiology of acute Respiratory infection in an Outpatient fever clinic during COVID-19 Pandemic in a Tertiary hospital in Nanjing","volume":"36","author":"Geng","year":"2022","journal-title":"China. J Clin Lab Anal"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.117002_bib0002","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1128\/CMR.00032-09","article-title":"Respiratory viral infections in infants: causes, clinical symptoms, virology, and immunology","volume":"23","author":"Tregoning","year":"2010","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.diagmicrobio.2025.117002_bib0003","doi-asserted-by":"crossref","DOI":"10.3389\/fpubh.2024.1371110","article-title":"The relationship between spiritual health and quality of life among COVID-19 patients with long-term complications in the post-coronavirus era","volume":"12","author":"Safarabadi","year":"2024","journal-title":"Front Public Health"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.117002_bib0004","doi-asserted-by":"crossref","first-page":"6319","DOI":"10.1038\/s41598-023-33627-9","article-title":"The epidemiological features of Respiratory tract infection using the multiplex panels detection during COVID-19 Pandemic in Shandong Province","volume":"13","author":"Zhang","year":"2023","journal-title":"China. Sci Rep"},{"issue":"7","key":"10.1016\/j.diagmicrobio.2025.117002_bib0005","doi-asserted-by":"crossref","DOI":"10.1002\/jmv.28899","article-title":"Dynamics of parainfluenza virus among hospitalized children with acute respiratory tract infection under two-child policy and COVID-19 pandemic in Hubei Province, China, 2014-2022","volume":"95","author":"Yi","year":"2023","journal-title":"J Med Virol"},{"key":"10.1016\/j.diagmicrobio.2025.117002_bib0006","doi-asserted-by":"crossref","DOI":"10.1177\/1178636118758651","article-title":"Viral and bacterial etiologies of acute Respiratory infections among children under 5\u2009years in Senegal","volume":"11","author":"Assane","year":"2018","journal-title":"Microbiol Insights"},{"issue":"9","key":"10.1016\/j.diagmicrobio.2025.117002_bib0007","doi-asserted-by":"crossref","first-page":"5310","DOI":"10.1002\/jmv.27102","article-title":"Viral coinfections in COVID-19","volume":"93","author":"Aghbash","year":"2021","journal-title":"J Med Virol"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2025.117002_bib0008","doi-asserted-by":"crossref","first-page":"e619","DOI":"10.1016\/S2214-109X(18)30186-4","article-title":"Antimicrobial consumption and resistance in adult hospital inpatients in 53 countries: results of an internet-based global point prevalence survey","volume":"6","author":"Versporten","year":"2018","journal-title":"Lancet Glob Health"},{"key":"10.1016\/j.diagmicrobio.2025.117002_bib0009","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2023.1269275","article-title":"Simultaneous detection of Human Norovirus GI, GII and SARS-CoV-2 by a quantitative one-step triplex RT-qPCR","volume":"14","author":"Yi","year":"2024","journal-title":"Front Microbiol"},{"issue":"11","key":"10.1016\/j.diagmicrobio.2025.117002_bib0010","doi-asserted-by":"crossref","DOI":"10.1099\/jmm.0.001771","article-title":"Performance of targeted next-generation sequencing in the detection of Respiratory pathogens and antimicrobial resistance genes for children","volume":"72","author":"Lin","year":"2023","journal-title":"J Med Microbiol"},{"key":"10.1016\/j.diagmicrobio.2025.117002_bib0011","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1146\/annurev-pathmechdis-012418-012751","article-title":"Clinical metagenomic next-generation sequencing for pathogen detection","volume":"14","author":"Gu","year":"2019","journal-title":"Annu Rev Pathol"},{"issue":"5","key":"10.1016\/j.diagmicrobio.2025.117002_bib0012","doi-asserted-by":"crossref","first-page":"e127","DOI":"10.1016\/j.jinf.2022.08.022","article-title":"Targeted next generation sequencing is comparable with metagenomic next generation sequencing in adults with pneumonia for pathogenic microorganism detection","volume":"85","author":"Li","year":"2022","journal-title":"J Infect"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.117002_bib0013","doi-asserted-by":"crossref","DOI":"10.1080\/22221751.2024.2392656","article-title":"Targeted next-generation sequencing of mycobacterium tuberculosis from patient samples: lessons learned from high drug-resistant burden clinical settings in Bangladesh","volume":"13","author":"Uddin","year":"2024","journal-title":"Emerg Microbes Infect"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.117002_bib0014","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s12985-020-01475-y","article-title":"Epidemiological characteristics of four common respiratory viral infections in children","volume":"18","author":"Zhu","year":"2021","journal-title":"Virol J"},{"key":"10.1016\/j.diagmicrobio.2025.117002_bib0015","doi-asserted-by":"crossref","DOI":"10.1016\/j.ebiom.2024.105307","article-title":"Enhancing lower respiratory tract infection diagnosis: implementation and clinical assessment of multiplex PCR-based and hybrid capture-based targeted next-generation sequencing","volume":"107","author":"Yin","year":"2024","journal-title":"EBioMedicine"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.117002_bib0016","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1186\/s12879-023-08312-x","article-title":"Demographic and clinical characteristics of Human bocavirus-1 infection in patients with acute Respiratory tract infections during the COVID-19 pandemic in the Central province of Sri Lanka","volume":"23","author":"Arunasalam","year":"2023","journal-title":"BMC Infect Dis"},{"issue":"8","key":"10.1016\/j.diagmicrobio.2025.117002_bib0017","doi-asserted-by":"crossref","first-page":"4748","DOI":"10.1002\/jmv.27004","article-title":"Epidemiology of acute Respiratory viral infections in children in Vientiane, Lao People's Democratic Republic","volume":"93","author":"Snoeck","year":"2021","journal-title":"J Med Virol"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2025.117002_bib0018","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.3390\/microorganisms8101516","article-title":"Congenital Cytomegalovirus infection: update on diagnosis and treatment","volume":"8","author":"Chiopris","year":"2020","journal-title":"Microorganisms"},{"key":"10.1016\/j.diagmicrobio.2025.117002_bib0019","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2024.1360075","article-title":"The effect of vitamin D deficiency on platelet parameters in patients with COVID-19","volume":"14","author":"Talebzadeh","year":"2024","journal-title":"Front Cell Infect Microbiol"},{"issue":"20","key":"10.1016\/j.diagmicrobio.2025.117002_bib0020","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.21037\/atm-22-4304","article-title":"Epidemic characteristics of Mycoplasma Pneumoniae infection: a retrospective analysis of a single center in Suzhou from 2014 to 2020","volume":"10","author":"Lv","year":"2022","journal-title":"Ann Transl Med"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2025.117002_bib0021","doi-asserted-by":"crossref","first-page":"fnv062","DOI":"10.1093\/femsle\/fnv062","article-title":"Viral bacterial co-infection of the respiratory tract during early childhood","volume":"362","author":"Brealey","year":"2015","journal-title":"FEMS Microbiol Lett"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2025.117002_bib0022","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1111\/j.1469-0691.2011.03603.x","article-title":"Viruses and bacteria in sputum samples of children with community-acquired pneumonia","volume":"18","author":"Honkinen","year":"2012","journal-title":"Clin Microbiol Infect"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.117002_bib0023","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1097\/INF.0b013e3181f111a2","article-title":"Association between nasopharyngeal load of Streptococcus pneumoniae, viral coinfection, and radiologically confirmed pneumonia in Vietnamese children","volume":"30","author":"Vu","year":"2011","journal-title":"Pediatr Infect Dis J"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.117002_bib0024","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1186\/s12879-021-05772-x","article-title":"Etiology, clinical characteristics and coinfection status of bronchiolitis in Suzhou","volume":"21","author":"Tan","year":"2021","journal-title":"BMC Infect Dis"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.117002_bib0025","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s12519-023-00793-9","article-title":"Current Mycoplasma Pneumoniae epidemic among children in Shanghai: unusual pneumonia caused by usual pathogen","volume":"20","author":"Zhang","year":"2024","journal-title":"World J Pediatr"},{"issue":"9","key":"10.1016\/j.diagmicrobio.2025.117002_bib0026","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1016\/j.cmi.2021.05.034","article-title":"The Association between bacteria colonizing the upper respiratory tract and lower respiratory tract infection in young children: a systematic review and meta-analysis","volume":"27","author":"Claassen-Weitz","year":"2021","journal-title":"Clin Microbiol Infect"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.117002_bib0027","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1146\/annurev-virology-012420-022445","article-title":"Seasonality of Respiratory viral infections","volume":"7","author":"Moriyama","year":"2020","journal-title":"Annu Rev Virol"},{"key":"10.1016\/j.diagmicrobio.2025.117002_bib0028","doi-asserted-by":"crossref","DOI":"10.1016\/j.curtheres.2024.100745","article-title":"Microbiological and molecular study of paranasal sinus infections of children with malignancy and unknown origin fever in Markazi Province, Iran","volume":"100","author":"Ghaffari","year":"2024","journal-title":"Curr Ther Res Clin Exp"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889325003256?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889325003256?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T02:17:12Z","timestamp":1762309032000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889325003256"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":28,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["S0732889325003256"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2025.117002","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Targeted next-generation sequencing for pathogen detection and epidemiological analysis of pediatric acute respiratory infections in Xiantao, China","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2025.117002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"117002"}}