{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T02:02:28Z","timestamp":1783648948531,"version":"3.55.0"},"reference-count":35,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.diagmicrobio.2025.117172","type":"journal-article","created":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:16:50Z","timestamp":1762273010000},"page":"117172","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Optimizing a fully automated real-time PCR assay on the cobas\u00ae 6800 Platform for detection of Candida auris in surveillance samples pre-treated with sonication"],"prefix":"10.1016","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3686-9691","authenticated-orcid":false,"given":"Eric Tin Long","family":"Lui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex Yat Man","family":"Ho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janice Sze Wing","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kin Ping","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wing Kin","family":"To","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wing Sze","family":"Cheung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viola Chi Ying","family":"Chow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shik","family":"Luk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.117172_bib0001","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/j.1348-0421.2008.00083.x","article-title":"Candida auris sp. nov., a novel ascomycetous yeast isolated from the external ear canal of an inpatient in a Japanese hospital","volume":"53","author":"Satoh","year":"2009","journal-title":"Microbiol Immunol"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.117172_bib0002","doi-asserted-by":"crossref","DOI":"10.1128\/CMR.00029-17","article-title":"Candida auris: a Review of the literature","volume":"31","author":"Jeffery-Smith","year":"2018","journal-title":"Clin Microbiol Rev"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.117172_bib0003","doi-asserted-by":"crossref","first-page":"2405","DOI":"10.1080\/22221751.2022.2125349","article-title":"Confirmation of fifth Candida auris clade by whole genome sequencing","volume":"11","author":"Spruijtenburg","year":"2022","journal-title":"Emerg Microbes Infect"},{"issue":"9","key":"10.1016\/j.diagmicrobio.2025.117172_bib0004","doi-asserted-by":"crossref","DOI":"10.1016\/S2666-5247(24)00101-0","article-title":"Detection and characterisation of a sixth <em>Candida auris<\/em>clade in Singapore: a genomic and phenotypic study","volume":"5","author":"Suphavilai","year":"2024","journal-title":"Lancet Microbe"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2025.117172_bib0005","first-page":"3","article-title":"Global emergence of a multi-drug resistant fungal pathogen, Candida auris","volume":"31","author":"Britz","year":"2016","journal-title":"South Afr J Infect Dis"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.117172_bib0006","doi-asserted-by":"crossref","DOI":"10.1128\/MRA.00308-20","article-title":"Draft genome sequences of 19 clinical isolates of Candida auris from Hong Kong","volume":"10","author":"Tse","year":"2021","journal-title":"Microbiol Resour Announc"},{"key":"10.1016\/j.diagmicrobio.2025.117172_bib0007","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.2147\/IDR.S451742","article-title":"The emergence of Candida auris is not associated with changes in antifungal prescription at hospitals","volume":"17","author":"Wong","year":"2024","journal-title":"Infect Drug Resist"},{"issue":"7","key":"10.1016\/j.diagmicrobio.2025.117172_bib0008","doi-asserted-by":"crossref","DOI":"10.1093\/mmy\/myae060","article-title":"Antifungal efficacy of natural antiseptic products against Candida auris","volume":"62","author":"Wu","year":"2024","journal-title":"Med Mycol"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2025.117172_bib0009","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1093\/cid\/civ933","article-title":"Clinical Practice Guideline for the management of Candidiasis: 2016 update by the Infectious Diseases Society of America","volume":"62","author":"Pappas","year":"2015","journal-title":"Clin Infect Dis"},{"issue":"5","key":"10.1016\/j.diagmicrobio.2025.117172_bib0010","doi-asserted-by":"crossref","first-page":"e280","DOI":"10.1016\/S1473-3099(24)00749-7","article-title":"Global guideline for the diagnosis and management of candidiasis: an initiative of the ECMM in cooperation with ISHAM and ASM","volume":"25","author":"Cornely","year":"2025","journal-title":"Lancet Infect Dis"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2025.117172_bib0011","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.chom.2020.12.002","article-title":"Murine model of colonization with fungal pathogen Candida auris to explore skin tropism, host risk factors and therapeutic strategies","volume":"29","author":"Huang","year":"2021","journal-title":"Cell Host Microbe"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.117172_bib0012","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.jhin.2019.06.006","article-title":"Candida auris exhibits resilient biofilm characteristics in vitro: implications for environmental persistence","volume":"103","author":"Short","year":"2019","journal-title":"J Hosp Infect"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2025.117172_bib0014","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01223-17","article-title":"Development and validation of a real-time PCR assay for rapid detection of Candida auris from surveillance samples","volume":"56","author":"Leach","year":"2018","journal-title":"J Clin Microbiol"},{"issue":"12","key":"10.1016\/j.diagmicrobio.2025.117172_bib0015","first-page":"56","article-title":"Direct detection of emergent fungal pathogen Candida auris in clinical skin swabs by SYBR green-based quantitative PCR assay","author":"Sexton","year":"2018","journal-title":"J Clin Microbiol"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2025.117172_bib0016","first-page":"57","article-title":"A rapid and automated sample-to-result Candida auris real-time PCR assay for high-throughput testing of surveillance samples with the BD Max Open system","author":"Leach","year":"2019","journal-title":"J Clin Microbiol"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.117172_bib0017","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-023-47220-7","article-title":"Streamlined instrument-free lysis for the detection of Candida auris","volume":"13","author":"Jin","year":"2023","journal-title":"Sci Rep"},{"key":"10.1016\/j.diagmicrobio.2025.117172_bib0018","unstructured":"Candida auris - Real-Time PCR Based Identification. Centers for Disease Control and Prevention; Available from: https:\/\/www.cdc.gov\/candida-auris\/hcp\/laboratories\/real-time-pcr-identification.html."},{"issue":"4","key":"10.1016\/j.diagmicrobio.2025.117172_bib0019","doi-asserted-by":"crossref","DOI":"10.1016\/j.jinf.2025.106476","article-title":"Rapid spread of Candida auris in China after COVID-19","volume":"90","author":"Bing","year":"2025","journal-title":"J Infect"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2025.117172_bib0020","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00371-20","article-title":"Candida auris phenotypic heterogeneity determines pathogenicity In vitro","volume":"5","author":"Brown","year":"2020","journal-title":"mSphere"},{"issue":"1 & 2","key":"10.1016\/j.diagmicrobio.2025.117172_bib0021","doi-asserted-by":"crossref","first-page":"88","DOI":"10.4103\/ijmr.IJMR_2304_20","article-title":"Pooled testing for COVID-19 diagnosis by real-time RT-PCR: a multi-site comparative evaluation of 5- & 10-sample pooling","volume":"152","author":"Praharaj","year":"2020","journal-title":"Indian J Med Res"},{"issue":"9150","key":"10.1016\/j.diagmicrobio.2025.117172_bib0022","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/S0140-6736(98)06318-1","article-title":"Feasibility and efficacy of routine PCR screening of blood donations for hepatitis C virus, hepatitis B virus, and HIV-1 in a blood-bank setting","volume":"353","author":"Roth","year":"1999","journal-title":"Lancet"},{"issue":"27","key":"10.1016\/j.diagmicrobio.2025.117172_bib0023","doi-asserted-by":"crossref","first-page":"3261","DOI":"10.1002\/sim.5334","article-title":"Pooled-testing procedures for screening high volume clinical specimens in heterogeneous populations","volume":"31","author":"Bilder","year":"2012","journal-title":"Stat Med"},{"key":"10.1016\/j.diagmicrobio.2025.117172_bib0024","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.00254-25","article-title":"Evaluation of a high-throughput Candidozyma auris assay for use on the cobas 5800\/6800\/8800 omni Utility Channel","author":"Alexander","year":"2025","journal-title":"Microbiol Spectr"},{"key":"10.1016\/j.diagmicrobio.2025.117172_bib0025","series-title":"Xpert\u00ae MRSA NxG package insert","year":"2025"},{"key":"10.1016\/j.diagmicrobio.2025.117172_bib0026","series-title":"cobas\u00ae 6800\/8800 systems operator's manual version 1.2","year":"2025"},{"key":"10.1016\/j.diagmicrobio.2025.117172_bib0027","unstructured":"CHROMagar. CHROMagar candida plus leaflet LF-EXT-073 [cited 2025 20\/06]; Available from: https:\/\/www.chromagar.com\/wp-content\/uploads\/2021\/11\/LF-EXT-073-V3.1.pdf."},{"key":"10.1016\/j.diagmicrobio.2025.117172_bib0028","series-title":"WHO fungal priority pathogens list to guide research, development and public health action","year":"2022"},{"issue":"8","key":"10.1016\/j.diagmicrobio.2025.117172_bib0029","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1016\/j.ajic.2023.01.012","article-title":"Candida auris admission screening pilot in select units of New York City health care facilities, 2017-2019","volume":"51","author":"Rowlands","year":"2023","journal-title":"Am J Infect Control"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2025.117172_bib0030","doi-asserted-by":"crossref","DOI":"10.1128\/jcm.00158-24","article-title":"Validation of a qualitative real-time PCR assay for the detection of Candida auris in hospital inpatient screening","volume":"62","author":"Franco","year":"2024","journal-title":"J Clin Microbiol"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2025.117172_bib0031","doi-asserted-by":"crossref","first-page":"e02114","DOI":"10.1128\/spectrum.02114-24","article-title":"Performance characteristics of an automated, high-throughput RT-PCR assay for the detection of Candida auris on 3-point and nasal swabs","volume":"13","author":"Nhan","year":"2025","journal-title":"Microbiol Spectr"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2025.117172_bib0032","doi-asserted-by":"crossref","first-page":"2996","DOI":"10.1128\/JCM.00921-17","article-title":"Survival, persistence, and isolation of the emerging multidrug-resistant pathogenic yeast Candida auris on a plastic health care surface","volume":"55","author":"Welsh","year":"2017","journal-title":"J Clin Microbiol"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2025.117172_bib0033","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/s13756-016-0132-5","article-title":"First hospital outbreak of the globally emerging Candida auris in a European hospital","volume":"5","author":"Schelenz","year":"2016","journal-title":"Antimicrob Resist Infect Control"},{"key":"10.1016\/j.diagmicrobio.2025.117172_bib0034","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2024.1398461","article-title":"Sonication protocols and their contributions to the microbiological diagnosis of implant-associated infections: a review of the current scenario","volume":"14","author":"Silva","year":"2024","journal-title":"Front Cell Infect Microbiol"},{"key":"10.1016\/j.diagmicrobio.2025.117172_bib0035","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/5584_2015_5007","article-title":"Role of sonication in the microbiological diagnosis of implant-associated infections: beyond the orthopedic prosthesis","volume":"897","author":"Oliva","year":"2016","journal-title":"Adv Exp Med Biol"},{"issue":"5","key":"10.1016\/j.diagmicrobio.2025.117172_bib0036","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.02055-18","article-title":"Candida auris isolates of the Southern Asian and South African lineages exhibit different phenotypic and antifungal susceptibility profiles In vitro","volume":"57","author":"Szekely","year":"2019","journal-title":"J Clin Microbiol"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889325004948?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889325004948?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T13:05:24Z","timestamp":1769519124000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889325004948"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":35,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["S0732889325004948"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2025.117172","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Optimizing a fully automated real-time PCR assay on the cobas\u00ae 6800 Platform for detection of Candida auris in surveillance samples pre-treated with sonication","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2025.117172","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"117172"}}