{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T07:09:58Z","timestamp":1776150598839,"version":"3.50.1"},"reference-count":36,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100023674","name":"King Khalid University Deanship of Scientific Research","doi-asserted-by":"publisher","award":["RGP.02\/725\/46"],"award-info":[{"award-number":["RGP.02\/725\/46"]}],"id":[{"id":"10.13039\/501100023674","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2026,7]]},"DOI":"10.1016\/j.diagmicrobio.2026.117386","type":"journal-article","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T16:46:17Z","timestamp":1773938777000},"page":"117386","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["A roadmap for rapid detection of tuberculosis through microfluidic lab-on-chip strategies"],"prefix":"10.1016","volume":"115","author":[{"given":"Iswarya","family":"Rambabu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thirunavukkarasu","family":"Palaniyandi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hemapreethi","family":"Surendran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandhya","family":"Nedunchezhian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdullah A.","family":"Aseeri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohd","family":"Saeed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarun Kumar","family":"Upadhyay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Manivannan S","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asha","family":"Sivaji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abimanyu","family":"Sugumaran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natarajan Alangudi","family":"Palaniappan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.diagmicrobio.2026.117386_bib59","series-title":"Global Tuberculosis Report 2025","year":"2025"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib0002","doi-asserted-by":"crossref","first-page":"5202","DOI":"10.3390\/ijms24065202","article-title":"Gunosewoyo tuberculosis: pathogenesis, current treatment regimens and new drug targets","volume":"24","author":"Alsayed","year":"2023","journal-title":"Int J Mol Sci"},{"issue":"34","key":"10.1016\/j.diagmicrobio.2026.117386_bib0001","first-page":"40","article-title":"Coll advances in rapid diagnosis of tuberculosis disease and anti-tuberculous drug resistance","volume":"29","author":"Alcaide","year":"2011","journal-title":"Enferm Infecc Microbiol Clin"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib0005","doi-asserted-by":"crossref","first-page":"427","DOI":"10.4161\/rna.20105","article-title":"Non-coding RNA and its potential role in M. tuberculosis pathogenesis","volume":"9","author":"Arnvig","year":"2012","journal-title":"RNA Biol"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib0051","article-title":"The PhoP-dependent ncRNA Mcr7 modulates the TAT secretion system in M.tuberculosis","volume":"10","author":"Solans","year":"2014","journal-title":"PLoSPathog"},{"issue":"25","key":"10.1016\/j.diagmicrobio.2026.117386_bib60","doi-asserted-by":"crossref","first-page":"6464","DOI":"10.1073\/pnas.1718003115","article-title":"Small RNA profiling in Mycobacterium tuberculosis identifies MrsI as necessary for an anticipatory iron sparing response","volume":"115","author":"Gerrick","year":"2018","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib61","doi-asserted-by":"crossref","first-page":"405","DOI":"10.3389\/fcimb.2019.00405","article-title":"Azhikina T.MTS1338, a small Mycobacterium tuberculosis RNA, regulates transcriptional shifts consistent with bacterial adaptation for entering into dormancy and survival within host macrophages","volume":"9","author":"Salina","year":"2019","journal-title":"Front Cell Infect Microbiol"},{"issue":"9","key":"10.1016\/j.diagmicrobio.2026.117386_bib62","doi-asserted-by":"crossref","first-page":"7928","DOI":"10.3390\/ijms24097928","article-title":"Small RNA MTS1338 configures a stress resistance signature in Mycobacterium tuberculosis","volume":"24","author":"Martini","year":"2023","journal-title":"Int J Mol Sci."},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib0004","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1111\/j.1365-2958.2009.06777.x","article-title":"Identification of small RNAs in mycobacterium tuberculosis","volume":"73","author":"Arnvig","year":"2009","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib0049","doi-asserted-by":"crossref","DOI":"10.1093\/femspd\/fty035","article-title":"Regulatory RNA in M. tuberculosis: back to basics","volume":"76","author":"Schwenk","year":"2018","journal-title":"Pathog Dis"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2026.117386_bib67","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1016\/j.celrep.2013.10.031","article-title":"Genome-wide mapping of transcriptional start sites defines an extensive leaderless transcriptome in Mycobacterium tuberculosis","volume":"5","author":"Cortes","year":"2013","journal-title":"Cell Rep"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib0028","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.gpb.2012.09.004","article-title":"Predicting sRNAs and their targets in bacteria","volume":"10","author":"Li","year":"2012","journal-title":"Genom Proteom Bioinform"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib0040","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1093\/femsre\/fuv016","article-title":"Target activation by regulatory RNAs in bacteria","volume":"39","author":"Papenfort","year":"2015","journal-title":"FEMS Microbiol Rev"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib0039","doi-asserted-by":"crossref","first-page":"S109","DOI":"10.1134\/S000629792114008X","article-title":"Small noncoding RNAs and their role in the pathogenesis of M. tuberculosis infection","volume":"86","author":"Ostrik","year":"2021","journal-title":"Biochem (Mosc)"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib68","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.gene.2018.02.068","article-title":"Small RNA-mediated regulation in bacteria: a growing palette of diverse mechanisms","volume":"656","author":"Dutta","year":"2018","journal-title":"Gene"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib0050","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1005641","article-title":"Leaderless transcripts and small proteins are common features of the mycobacterial translational landscape","volume":"11","author":"Shell","year":"2015","journal-title":"PLoS Genet"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib0048","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2022.976550","article-title":"Ribosome profiling enhances understanding of mycobacterial translation","volume":"13","author":"Sawyer","year":"2022","journal-title":"Front Microbiol"},{"issue":"20","key":"10.1016\/j.diagmicrobio.2026.117386_bib70","first-page":"7668","article-title":"Identification of functional Tat signal sequences in Mycobacterium tuberculosis proteins","volume":"187","author":"McDonough","year":"2005","journal-title":"J Bacteriol"},{"issue":"20","key":"10.1016\/j.diagmicrobio.2026.117386_bib71","first-page":"675","article-title":"Genetic analysis of the \u03b2-lactamases of Mycobacterium tuberculosis","volume":"187","author":"Flores","year":"2005","journal-title":"J Bacteriol"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2026.117386_bib69","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0174079","article-title":"Expression, maturation and turnover of DrrS, an unusually stable DosR-regulated small RNA in Mycobacterium tuberculosis","volume":"12","author":"Moores","year":"2017","journal-title":"PLoS One"},{"issue":"5","key":"10.1016\/j.diagmicrobio.2026.117386_bib0027","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1039\/D4LC00117F","article-title":"Microfluidic systems for infectious disease diagnostics","volume":"24","author":"Lehnert","year":"2024","journal-title":"Lab Chip"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib0045","doi-asserted-by":"crossref","first-page":"13988","DOI":"10.1002\/anie.201309580","article-title":"Microfluidic isolation of nucleic acids","volume":"53","author":"Reinholt","year":"2014","journal-title":"Angew Chem Int Ed"},{"issue":"11","key":"10.1016\/j.diagmicrobio.2026.117386_bib0007","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1056\/NEJMoa0907847","article-title":"Rapid molecular detection of tuberculosis and rifampin resistance","volume":"363","author":"Boehme","year":"2010","journal-title":"N Engl J Med"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2026.117386_bib0038","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0051121","article-title":"Rapid diagnosis of mycobacterium tuberculosis with Truenat MTB: a near-care approach","volume":"8","author":"Nikam","year":"2013","journal-title":"PLoS One"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2026.117386_bib0026","doi-asserted-by":"crossref","first-page":"2616","DOI":"10.1128\/JCM.41.6.2616-2622.2003","article-title":"Loop-mediated isothermal amplification for direct detection of mycobacterium tuberculosis complex, M. avium, and M. intracellulare in sputum samples","volume":"41","author":"Iwamoto","year":"2003","journal-title":"J Clin Microbiol"},{"issue":"12","key":"10.1016\/j.diagmicrobio.2026.117386_bib72","doi-asserted-by":"crossref","first-page":"e63","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib0030","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/1748-7188-6-26","article-title":"ViennaRNA package 2.0","volume":"6","author":"Lorenz","year":"2011","journal-title":"Algorithms Mol Biol"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib0037","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1021\/acs.analchem.9b04986","article-title":"Microfluidics: innovations in materials and their fabrication and functionalization","volume":"92","author":"Nielsen","year":"2019","journal-title":"Anal Chem"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2026.117386_bib0036","doi-asserted-by":"crossref","first-page":"2011","DOI":"10.3390\/ijms22042011","article-title":"Fabrication and applications of microfluidic devices: a review","volume":"22","author":"Niculescu","year":"2021","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib64","article-title":"Polydimethylsiloxane-based microfluidic platforms for molecular diagnostics","volume":"144","author":"Choi","year":"2019","journal-title":"Biosens Bioelectron"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib0012","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/0003-2697(87)90021-2","article-title":"Single-step method of RNA isolation by acid guanidinium thiocyanate\u2013phenol\u2013chloroform extraction","volume":"162","author":"Chomczynski","year":"1987","journal-title":"Anal Biochem"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2026.117386_bib65","doi-asserted-by":"crossref","DOI":"10.1063\/1.5088552","article-title":"A point-of-care platform based on microfluidic chip for nucleic acid extraction in less than 1 minute","volume":"13","author":"Zhang","year":"2019","journal-title":"Biomicrofluidics"},{"issue":"17","key":"10.1016\/j.diagmicrobio.2026.117386_bib66","doi-asserted-by":"crossref","first-page":"2769","DOI":"10.1039\/C9LC00389D","article-title":"Integrated microfluidic systems with sample preparation and nucleic acid amplification","volume":"19","author":"Yin","year":"2019","journal-title":"Lab Chip"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib63","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/s10544-008-9240-1","article-title":"Magnetic-bead-based microfluidic system for ribonucleic acid extraction and reverse transcription processes","volume":"11","author":"Liu","year":"2009","journal-title":"Biomed Microdevices"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib74","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1146\/annurev-biophys-070816-034058","article-title":"Integration of bacterial small RNAs in regulatory networks","volume":"46","author":"Nitzan","year":"2017","journal-title":"Annu Rev Biophys"},{"key":"10.1016\/j.diagmicrobio.2026.117386_bib0006","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1021\/ac051449j","article-title":"Thermoplastic microfluidic device for on-chip purification of nucleic acids for disposable diagnostics","volume":"78","author":"Bhattacharyya","year":"2006","journal-title":"Anal Chem"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889326001367?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889326001367?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T06:32:25Z","timestamp":1776148345000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889326001367"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":36,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["S0732889326001367"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2026.117386","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A roadmap for rapid detection of tuberculosis through microfluidic lab-on-chip strategies","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2026.117386","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"117386"}}