{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:17:50Z","timestamp":1792653470876,"version":"4.5.0"},"reference-count":44,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023A1515011906"],"award-info":[{"award-number":["2023A1515011906"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018623","name":"Quanzhou City Science and Technology Program","doi-asserted-by":"publisher","award":["2025QZC02DW"],"award-info":[{"award-number":["2025QZC02DW"]}],"id":[{"id":"10.13039\/501100018623","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2023J011787"],"award-info":[{"award-number":["2023J011787"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009047","name":"Shantou University","doi-asserted-by":"publisher","award":["NTF20030"],"award-info":[{"award-number":["NTF20030"]}],"id":[{"id":"10.13039\/100009047","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010166","name":"Xiamen Municipal Bureau of Science and Technology","doi-asserted-by":"publisher","award":["Y20250070"],"award-info":[{"award-number":["Y20250070"]}],"id":[{"id":"10.13039\/100010166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.diagmicrobio.2026.117453","type":"journal-article","created":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T15:05:44Z","timestamp":1777993544000},"page":"117453","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["Rapid, portable, one-pot CRISPR\/Dx system for multiplex detection of human rhinovirus, adenovirus, and Mycoplasma pneumoniae"],"prefix":"10.1016","volume":"116","author":[{"given":"Yumei","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4528-0363","authenticated-orcid":false,"given":"Cia-Hin","family":"Lau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqing","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyuan","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibao","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"9","key":"10.1016\/j.diagmicrobio.2026.117453_bib0001","article-title":"Epidemiology and diagnostic accuracy of respiratory pathogens in pediatric populations: insights from global studies","volume":"16","author":"Bulata-Pop","year":"2024","journal-title":"Cureus"},{"issue":"7","key":"10.1016\/j.diagmicrobio.2026.117453_bib0002","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.00079-25","article-title":"Epidemiological characteristics of six common respiratory pathogen infections in children","volume":"13","author":"Tang","year":"2025","journal-title":"Microbiol Spectr"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2026.117453_bib0003","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1016\/j.jaci.2010.04.010","article-title":"Analysis of the complete genome sequences of human rhinovirus","volume":"125","author":"Palmenberg","year":"2010","journal-title":"J Allergy Clin Immunol"},{"key":"10.1016\/j.diagmicrobio.2026.117453_bib0004","doi-asserted-by":"crossref","first-page":"2020","DOI":"10.2741\/2207","article-title":"Mycoplasma genomics: tailoring the genome for minimal life requirements through reductive evolution","volume":"12","author":"Fadiel","year":"2007","journal-title":"Front Biosci"},{"key":"10.1016\/j.diagmicrobio.2026.117453_bib0005","doi-asserted-by":"crossref","first-page":"2178","DOI":"10.3389\/fmicb.2018.02178","article-title":"Adenoviromics: mining the human adenovirus species D genome","volume":"9","author":"Ismail","year":"2018","journal-title":"Front Microbiol"},{"issue":"7","key":"10.1016\/j.diagmicrobio.2026.117453_bib0006","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1515\/cclm-2024-0962","article-title":"Artificial base mismatches-mediated PCR (ABM-PCR) for detecting clinically relevant single-base mutations","volume":"63","author":"Lau","year":"2025","journal-title":"Clin Chem Lab Med"},{"issue":"31","key":"10.1016\/j.diagmicrobio.2026.117453_bib0007","doi-asserted-by":"crossref","first-page":"6304","DOI":"10.1039\/D5AY00783F","article-title":"Rapid, multiplex, one-pot CRISPR\/Dx system for detecting cancer fusion genes","volume":"17","author":"Li","year":"2025","journal-title":"Anal Methods"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2026.117453_bib0008","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1039\/D4AY01695E","article-title":"One-pot isothermal CRISPR\/dx system for specific and sensitive detection of microRNA","volume":"17","author":"Xinyu","year":"2025","journal-title":"Analytical Methods"},{"key":"10.1016\/j.diagmicrobio.2026.117453_bib0009","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2025.117778","article-title":"CRISPR\/Dx-based colorimetric and electrochemical detection systems for POCT applications","volume":"288","author":"Lau","year":"2025","journal-title":"Biosens Bioelectron"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2026.117453_bib0010","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.tibtech.2025.07.006","article-title":"CRISPR\/Cas-based personal glucose meters for nucleic acid detection","volume":"44","author":"Li","year":"2026","journal-title":"Trends Biotechnol"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2026.117453_bib0011","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1080\/07388551.2024.2399560","article-title":"Amplification-free nucleic acids detection with next-generation CRISPR\/dx systems","volume":"45","author":"Lau","year":"2025","journal-title":"Crit Rev Biotechnol"},{"key":"10.1016\/j.diagmicrobio.2026.117453_bib0012","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.omtm.2022.05.005","article-title":"PAM-flexible dual base editor-mediated random mutagenesis and self-activation strategies to improve CRISPRa potency","volume":"26","author":"Lau","year":"2022","journal-title":"Mol Ther Methods Clin Dev"},{"key":"10.1016\/j.diagmicrobio.2026.117453_bib0013","article-title":"CRISPR technology for diagnosis and treatment of human brucellosis","volume":"240","author":"Lau","year":"2025","journal-title":"J Microbiol Methods"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2026.117453_bib0014","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1089\/crispr.2018.0026","article-title":"Applications of CRISPR-Cas in bioengineering, biotechnology, and translational research","volume":"1","author":"Lau","year":"2018","journal-title":"CRISPR J"},{"issue":"26","key":"10.1016\/j.diagmicrobio.2026.117453_bib0015","doi-asserted-by":"crossref","first-page":"28866","DOI":"10.1021\/acsomega.4c03413","article-title":"Rapid and amplification-free nucleic acid detection with DNA substrate-mediated autocatalysis of CRISPR\/Cas12a","volume":"9","author":"Zhou","year":"2024","journal-title":"ACS Omega"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2026.117453_bib0016","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1039\/D5AY01957E","article-title":"A Bst-driven Cas12a cascade amplification strategy for microRNA detection","volume":"18","author":"Xu","year":"2026","journal-title":"Anal Methods"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2026.117453_bib0017","doi-asserted-by":"crossref","first-page":"2303","DOI":"10.1021\/acs.analchem.2c03990","article-title":"CRISPR Cas12a-powered silicon surface-enhanced raman spectroscopy ratiometric chip for sensitive and reliable quantification","volume":"95","author":"Cao","year":"2023","journal-title":"Anal Chem"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2026.117453_bib0018","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1021\/acs.analchem.3c04866","article-title":"Self-priming cyclic amplification accelerating CRISPR sensor for sensitive and specific MicroRNA analysis with No background","volume":"96","author":"Li","year":"2024","journal-title":"Anal Chem"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2026.117453_bib0019","doi-asserted-by":"crossref","DOI":"10.1002\/ardp.202300005","article-title":"Zinc(II) complexation strategy for ultra-sensitive fluorimetric estimation of molnupiravir: applications and greenness evaluation","volume":"356","author":"Salman","year":"2023","journal-title":"Arch Pharm (Weinheim)"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2026.117453_bib0020","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1039\/D4AY01695E","article-title":"One-pot isothermal CRISPR\/dx system for specific and sensitive detection of microRNA","volume":"17","author":"Li","year":"2025","journal-title":"Anal Methods"},{"issue":"49","key":"10.1016\/j.diagmicrobio.2026.117453_bib0021","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2113747118","article-title":"Mg(2+)-dependent conformational rearrangements of CRISPR-Cas12a R-loop complex are mandatory for complete double-stranded DNA cleavage","volume":"118","author":"Son","year":"2021","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"16","key":"10.1016\/j.diagmicrobio.2026.117453_bib0022","doi-asserted-by":"crossref","first-page":"9343","DOI":"10.1093\/nar\/gkae613","article-title":"CRISPR-Cas12a exhibits metal-dependent specificity switching","volume":"52","author":"Nguyen","year":"2024","journal-title":"Nucleic Acids Res"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2026.117453_bib0023","doi-asserted-by":"crossref","first-page":"3617","DOI":"10.1021\/acs.analchem.4c06107","article-title":"CrRNA conformation-engineered CRISPR-Cas12a system for robust and ultrasensitive nucleic acid detection","volume":"97","author":"Li","year":"2025","journal-title":"Anal Chem"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2026.117453_bib0024","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1186\/s12866-023-03096-1","article-title":"Establishment of RT-RPA-Cas12a assay for rapid and sensitive detection of human rhinovirus B","volume":"23","author":"Li","year":"2023","journal-title":"BMC Microbiol"},{"key":"10.1016\/j.diagmicrobio.2026.117453_bib0025","doi-asserted-by":"crossref","DOI":"10.1016\/j.virusres.2022.199001","article-title":"Sensitive and rapid RT-RPA-Cas12a-mediated detection method capable of human rhinovirus A and\/or C species by targeting VP4","volume":"323","author":"Qian","year":"2023","journal-title":"Virus Res"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2026.117453_bib0026","doi-asserted-by":"crossref","DOI":"10.1177\/03000605231223083","article-title":"A multiplex recombinase polymerase amplification assay combined with CRISPR\/Cas12a for the detection of respiratory syncytial virus and respiratory adenovirus","volume":"52","author":"Hongdan","year":"2024","journal-title":"J Int Med Res"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2026.117453_bib0027","doi-asserted-by":"crossref","DOI":"10.1016\/j.imj.2025.100181","article-title":"Rapid and sensitive detection of human adenovirus types 3 and 7 using CRISPR-Cas12b coupled with multiple cross displacement amplification","volume":"4","author":"Jia","year":"2025","journal-title":"Infect Med"},{"key":"10.1016\/j.diagmicrobio.2026.117453_bib0028","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2025.1607974","article-title":"Recombinase polymerase amplification combined with CRISPR\/Cas12a technology for rapid on-site detection of duck adenovirus 3","volume":"16","author":"Liang","year":"2025","journal-title":"Front Microbiol"},{"key":"10.1016\/j.diagmicrobio.2026.117453_bib0029","doi-asserted-by":"crossref","DOI":"10.1016\/j.mimet.2025.107314","article-title":"A rapid and visual detection for canine Adenovirus-2 using CRISPR-Cas13a-based SHERLOCK technology","volume":"239","author":"Liu","year":"2025","journal-title":"J Microbiol Methods"},{"issue":"9","key":"10.1016\/j.diagmicrobio.2026.117453_bib0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2024.104048","article-title":"A rapid, ultrasensitive, and highly specific method for detecting fowl adenovirus serotype 4 based on the LAMP-CRISPR\/Cas12a system","volume":"103","author":"Yu","year":"2024","journal-title":"Poult Sci"},{"issue":"21","key":"10.1016\/j.diagmicrobio.2026.117453_bib0031","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.3390\/diagnostics15212725","article-title":"An ERA-CRISPR\/Cas12a method for highly sensitive detection of Human Adenovirus type 55","volume":"15","author":"Zhang","year":"2025","journal-title":"Diagnostics (Basel)"},{"key":"10.1016\/j.diagmicrobio.2026.117453_bib0032","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2022.811768","article-title":"Ultrasensitive, specific, and rapid detection of Mycoplasma pneumoniae using the ERA\/CRISPR-Cas12a Dual system","volume":"13","author":"Deng","year":"2022","journal-title":"Front Microbiol"},{"key":"10.1016\/j.diagmicrobio.2026.117453_bib0033","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2025.117839","article-title":"A novel thermo-activated one-pot RPA-CRISPR-Cas12b assay for Mycoplasma pneumoniae POCT","volume":"288","author":"Feng","year":"2025","journal-title":"Biosens Bioelectron"},{"issue":"37","key":"10.1016\/j.diagmicrobio.2026.117453_bib0034","doi-asserted-by":"crossref","first-page":"38989","DOI":"10.1021\/acsomega.4c05489","article-title":"CHAMP: a centrifugal microfluidics-based CRISPR\/Cas12b-combined real-time LAMP one-pot method for Mycoplasma pneumoniae infection diagnosis","volume":"9","author":"Jing","year":"2024","journal-title":"ACS Omega"},{"key":"10.1016\/j.diagmicrobio.2026.117453_bib0035","article-title":"Development of a rapid and efficient RPA-CRISPR\/Cas12a assay for Mycoplasma pneumoniae detection","volume":"13","author":"Li","year":"2022","journal-title":"Front Microbiol"},{"key":"10.1016\/j.diagmicrobio.2026.117453_bib0036","doi-asserted-by":"crossref","DOI":"10.1016\/j.cca.2024.119906","article-title":"Establishment of a rapid detection method for Mycoplasma pneumoniae based on RPA-CRISPR-Cas12a technology","volume":"564","author":"Li","year":"2025","journal-title":"Clin Chim Acta"},{"key":"10.1016\/j.diagmicrobio.2026.117453_bib0037","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2025.117458","article-title":"Palm-sized CRISPR sensing platform for on-site Mycoplasma pneumoniae detection","volume":"281","author":"Li","year":"2025","journal-title":"Biosens Bioelectron"},{"key":"10.1016\/j.diagmicrobio.2026.117453_bib0038","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2024.1409078","article-title":"An accurate and convenient method for Mycoplasma pneumoniae via one-step LAMP-CRISPR\/Cas12b detection platform","volume":"14","author":"Liu","year":"2024","journal-title":"Front Cell Infect Microbiol"},{"issue":"97","key":"10.1016\/j.diagmicrobio.2026.117453_bib0039","doi-asserted-by":"crossref","first-page":"19241","DOI":"10.1039\/D5CC04993H","article-title":"CRISPR-coupled triple cascade amplification for simultaneous lateral flow detection of Mycoplasma pneumoniae and H1N1","volume":"61","author":"Wei","year":"2025","journal-title":"Chem Commun (Camb)"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2026.117453_bib0040","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.03235-24","article-title":"The establishment and optimization of a Mycoplasma pneumoniae detection system based on ERA-CRISPR\/Cas12a","volume":"13","author":"Yang","year":"2025","journal-title":"Microbiol Spectr"},{"key":"10.1016\/j.diagmicrobio.2026.117453_bib0041","doi-asserted-by":"crossref","DOI":"10.1016\/j.cca.2025.120274","article-title":"Considerations for enhancing the clinical validation and comparative analysis of RPA-CRISPR-Cas12a in Mycoplasma pneumoniae detection","volume":"572","author":"Yuan","year":"2025","journal-title":"Clin Chim Acta"},{"key":"10.1016\/j.diagmicrobio.2026.117453_bib0042","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2025.117196","article-title":"Amplification-free detection of mycoplasma pneumoniae via CRISPR-Cas12a and deep learning-optimized crRNAs on a lateral flow platform","volume":"268","author":"Zhou","year":"2026","journal-title":"J Pharm Biomed Anal"},{"key":"10.1016\/j.diagmicrobio.2026.117453_bib0043","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2023.1147142","article-title":"Rapid, ultrasensitive and highly specific diagnosis of Mycoplasma pneumoniae by a CRISPR-based detection platform","volume":"13","author":"Zhou","year":"2023","journal-title":"Front Cell Infect Microbiol"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2026.117453_bib0044","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1186\/s12985-025-02938-w","article-title":"Development of a single-tube, dual-target CRISPR Cas12a\/Cas13a system for rapid screening of coinfection with respiratory syncytial virus and rhinovirus","volume":"22","author":"Zhao","year":"2025","journal-title":"Virol J"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889326002038?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889326002038?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T10:39:21Z","timestamp":1783075161000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889326002038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":44,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["S0732889326002038"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2026.117453","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Rapid, portable, one-pot CRISPR\/Dx system for multiplex detection of human rhinovirus, adenovirus, and Mycoplasma pneumoniae","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2026.117453","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"117453"}}