{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:51:57Z","timestamp":1791244317876,"version":"4.1.0"},"reference-count":37,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.diagmicrobio.2026.117482","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T23:44:30Z","timestamp":1779925470000},"page":"117482","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of the performance of reverse transcription-recombinase polymerase amplification (RT-RPA) coupled with CRISPR\/Cas12a and microfluidics for one-step detection of common HCV genotypes"],"prefix":"10.1016","volume":"116","author":[{"given":"Qiong","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yushu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4032-7879","authenticated-orcid":false,"given":"Fuliang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.diagmicrobio.2026.117482_bib0001","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1002\/hep.27259","article-title":"Global distribution and prevalence of hepatitis c virus genotypes","volume":"61","author":"Messina","year":"2015","journal-title":"Hepatology"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2026.117482_bib0002","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1146\/annurev-med-050223-111239","article-title":"Global elimination of hepatitis c virus","volume":"76","author":"Fleurence","year":"2025","journal-title":"Annu Rev Med"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2026.117482_bib0003","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1002\/hep.26141","article-title":"Global epidemiology of hepatitis c virus infection: new estimates of age-specific antibody to HCV seroprevalence","volume":"57","author":"Mohd Hanafiah","year":"2013","journal-title":"Hepatology"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2026.117482_bib0004","first-page":"5646","article-title":"Epidemiology, transmission, diagnosis, and outcome of hepatitis c virus infection","volume":"9","author":"Moosavy","year":"2017","journal-title":"Electron Phys"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2026.117482_bib0005","doi-asserted-by":"crossref","first-page":"394","DOI":"10.3350\/cmh.2024.0664","article-title":"Cost-effectiveness and return on investment of hepatitis c virus elimination in China: a modelling study","volume":"31","author":"Wu","year":"2025","journal-title":"Clin Mol Hepatol"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2026.117482_bib0006","doi-asserted-by":"crossref","first-page":"3738","DOI":"10.1002\/jmv.26433","article-title":"Hepatitis c virus antigen detection is an appropriate test for screening and early diagnosis of hepatitis c virus infection in at-risk populations and immunocompromised hosts","volume":"93","author":"Kumar","year":"2021","journal-title":"J Med Virol"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2026.117482_bib0007","doi-asserted-by":"crossref","DOI":"10.1128\/jcm.00832-24","article-title":"Comparison of a dual antibody and antigen HCV immunoassay to standard of care algorithmic testing","volume":"62","author":"Bui","year":"2024","journal-title":"J Clin Microbiol"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2026.117482_bib0008","doi-asserted-by":"crossref","first-page":"3423","DOI":"10.3390\/s18103423","article-title":"HCV detection, discrimination, and genotyping technologies","volume":"18","author":"Warkad","year":"2018","journal-title":"Sensors"},{"key":"10.1016\/j.diagmicrobio.2026.117482_bib0009","doi-asserted-by":"crossref","DOI":"10.1016\/j.meegid.2024.105653","article-title":"Hepatitis c virus subtype distribution and resistance-associated substitutions in high-risk population groups in Guangdong province, China","volume":"123","author":"Xie","year":"2024","journal-title":"Infect Genet Evol"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2026.117482_bib0010","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.cmi.2021.06.042","article-title":"Clinical evaluation of a hepatitis c virus whole-genome sequencing pipeline for genotyping and resistance testing","volume":"28","author":"Bradshaw","year":"2022","journal-title":"Clin Microbiol Infect"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2026.117482_bib0011","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-024-64209-y","article-title":"On leveraging self-supervised learning for accurate HCV genotyping","volume":"14","author":"Fahmy","year":"2024","journal-title":"Sci Rep"},{"issue":"7","key":"10.1016\/j.diagmicrobio.2026.117482_bib0012","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1373\/clinchem.2015.245829","article-title":"Recombinase polymerase amplification for diagnostic applications","volume":"62","author":"Daher","year":"2016","journal-title":"Clin Chem"},{"key":"10.1016\/j.diagmicrobio.2026.117482_bib0013","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.1019071","article-title":"Recent advances in recombinase polymerase amplification: principle, advantages, disadvantages and applications","volume":"12","author":"Tan","year":"2022","journal-title":"Front Cell Infect Microbiol"},{"issue":"6","key":"10.1016\/j.diagmicrobio.2026.117482_bib0014","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1139\/cjm-2021-0329","article-title":"Recombinase polymerase amplification in the molecular diagnosis of microbiological targets and its applications","volume":"68","author":"Mota","year":"2022","journal-title":"Can J Microbiol"},{"key":"10.1016\/j.diagmicrobio.2026.117482_bib0015","first-page":"1","article-title":"Crispr\/cas systems for the detection of nucleic acid and non-nucleic acid targets","author":"Su","year":"2023","journal-title":"Nano Res"},{"issue":"10","key":"10.1016\/j.diagmicrobio.2026.117482_bib0016","doi-asserted-by":"crossref","DOI":"10.3390\/diagnostics12102455","article-title":"Application of crispr\/cas systems in the nucleic acid detection of infectious diseases","volume":"12","author":"Li","year":"2022","journal-title":"Diagnostics"},{"issue":"19","key":"10.1016\/j.diagmicrobio.2026.117482_bib0017","doi-asserted-by":"crossref","first-page":"12156","DOI":"10.1021\/acs.analchem.9b01526","article-title":"Cas12avdet: a Crispr\/cas12a-based platform for rapid and visual nucleic acid detection","volume":"91","author":"Wang","year":"2019","journal-title":"Anal Chem"},{"issue":"6336","key":"10.1016\/j.diagmicrobio.2026.117482_bib0018","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1126\/science.aam9321","article-title":"Nucleic acid detection with crispr-cas13a\/c2c2","volume":"356","author":"Gootenberg","year":"2017","journal-title":"Science"},{"issue":"5","key":"10.1016\/j.diagmicrobio.2026.117482_bib0019","doi-asserted-by":"crossref","first-page":"287","DOI":"10.18097\/pbmc20247005287","article-title":"Biosensing platforms for DNA diagnostics based on Crispr\/Cas nucleases: towards the detection of nucleic acids at the level of single molecules in non-laboratory settings","volume":"70","author":"Khmeleva","year":"2024","journal-title":"Biomed Khim"},{"issue":"11","key":"10.1016\/j.diagmicrobio.2026.117482_bib0020","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.3390\/mi13111923","article-title":"Integrating microfluidics and electronics in point-of-care diagnostics: current and future challenges","volume":"13","author":"Annese","year":"2022","journal-title":"Micromachines"},{"issue":"12","key":"10.1016\/j.diagmicrobio.2026.117482_bib0021","doi-asserted-by":"crossref","first-page":"2091","DOI":"10.1039\/b814922d","article-title":"Development of a digital microfluidic platform for point of care testing","volume":"8","author":"Sista","year":"2008","journal-title":"Lab Chip"},{"key":"10.1016\/j.diagmicrobio.2026.117482_bib0022","doi-asserted-by":"crossref","unstructured":"Athar M.A., Ahmad V., Ullah I., et al. Molecular diagnosis of hepatitis c viruses; technologies and its clinical applications review. 2019.","DOI":"10.20944\/preprints201810.0158.v1"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2026.117482_bib0023","doi-asserted-by":"crossref","first-page":"240","DOI":"10.4103\/1319-3767.182461","article-title":"Hepatitis c virus genotype 4 replication in the hepatocellular carcinoma cell line hepg2\/c3a","volume":"22","author":"Shier","year":"2016","journal-title":"Saudi J Gastroenterol"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2026.117482_bib0024","article-title":"The principles of biomedical scientific writing: materials and methods","volume":"17","author":"Ghasemi","year":"2019","journal-title":"Int J Endocrinol Metab"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2026.117482_bib0025","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1186\/s12967-021-02741-5","article-title":"One-tube sars-cov-2 detection platform based on rt-RPA and Crispr\/cas12a","volume":"19","author":"Sun","year":"2021","journal-title":"J Transl Med"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2026.117482_bib0026","doi-asserted-by":"crossref","DOI":"10.1002\/imt2.214","article-title":"Deep learning enhancing guide RNA design for Crispr\/cas12a-based diagnostics","volume":"3","author":"Huang","year":"2024","journal-title":"iMeta"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2026.117482_bib0027","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/s11658-024-00548-y","article-title":"A multiplex RPA-Crispr\/cas12a-based poct technique and its application in human papillomavirus (HPV) typing assay","volume":"29","author":"Liu","year":"2024","journal-title":"Cell Mol Biol Lett"},{"key":"10.1016\/j.diagmicrobio.2026.117482_bib0028","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2023.1158672","article-title":"Crispr-cas technology: a key approach for sars-cov-2 detection","volume":"11","author":"Fang","year":"2023","journal-title":"Front Bioeng Biotechnol"},{"issue":"2","key":"10.1016\/j.diagmicrobio.2026.117482_bib0029","doi-asserted-by":"crossref","DOI":"10.1016\/j.bj.2023.02.007","article-title":"Sars-cov-2 pandemics: an update of Crispr in diagnosis and host-virus interaction studies","volume":"46","author":"Tang","year":"2023","journal-title":"Biomed J"},{"issue":"6387","key":"10.1016\/j.diagmicrobio.2026.117482_bib0030","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1126\/science.aar6245","article-title":"Crispr-cas12a target binding unleashes indiscriminate single-stranded DNASE activity","volume":"360","author":"Chen","year":"2018","journal-title":"Science"},{"issue":"7","key":"10.1016\/j.diagmicrobio.2026.117482_bib0031","doi-asserted-by":"crossref","DOI":"10.3390\/diagnostics12071524","article-title":"Highly specific and rapid detection of hepatitis c virus using rt-lamp-coupled crispr-cas12 assay","volume":"12","author":"Kham-Kjing","year":"2022","journal-title":"Diagnostics"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2026.117482_bib0032","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens12010056","article-title":"A landscape of Crispr\/Cas technique for emerging viral disease diagnostics and therapeutics: progress and prospects","volume":"12","author":"Tripathi","year":"2022","journal-title":"Pathogens"},{"issue":"3","key":"10.1016\/j.diagmicrobio.2026.117482_bib0033","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1053\/j.gastro.2011.06.004","article-title":"Differential efficacy of protease inhibitors against HCV genotypes 2a, 3a, 5a, and 6a ns3\/4a protease recombinant viruses","volume":"141","author":"Gottwein","year":"2011","journal-title":"Gastroenterology"},{"issue":"4","key":"10.1016\/j.diagmicrobio.2026.117482_bib0034","doi-asserted-by":"crossref","DOI":"10.1016\/j.heliyon.2024.e26179","article-title":"Crispr\/cas-based nucleic acid detection strategies: trends and challenges","volume":"10","author":"Zhou","year":"2024","journal-title":"Heliyon"},{"key":"10.1016\/j.diagmicrobio.2026.117482_bib0035","article-title":"Iscan-v2: a one-pot rt-RPA-Crispr\/cas12b assay for point-of-care sars-cov-2 detection","volume":"9","author":"Aman","year":"2021","journal-title":"Front Bioeng Biotechnol"},{"issue":"1","key":"10.1016\/j.diagmicrobio.2026.117482_bib0036","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1186\/s12879-021-06678-4","article-title":"Application and evaluation of nucleic acid sequence-based amplification, PCR and cryptococcal antigen test for diagnosis of cryptococcosis","volume":"21","author":"Wang","year":"2021","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2026.117482_bib0037","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2024.343396","article-title":"Microfluidic-based fluorescence enhancement of silica-embedded carbon dots for direct detection and quantification of unamplified HCV RNA in clinical samples","volume":"1333","author":"Shaat","year":"2025","journal-title":"Anal Chim Acta"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889326002324?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889326002324?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:00:34Z","timestamp":1791241234000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889326002324"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":37,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["S0732889326002324"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2026.117482","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Evaluation of the performance of reverse transcription-recombinase polymerase amplification (RT-RPA) coupled with CRISPR\/Cas12a and microfluidics for one-step detection of common HCV genotypes","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2026.117482","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"117482"}}