{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:54:16Z","timestamp":1791244456121,"version":"4.1.0"},"reference-count":49,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.diagmicrobio.2026.117559","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T15:05:20Z","timestamp":1784214320000},"page":"117559","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Selective pressure-driven identification of conserved regions for Oropouche virus molecular detection"],"prefix":"10.1016","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6238-7237","authenticated-orcid":false,"given":"Andrea","family":"Avendano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Mavian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandro","family":"Balzano-Nogueira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Ait Said","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Salemi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Paniz-Mondolfi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0001","doi-asserted-by":"crossref","first-page":"439","DOI":"10.3390\/v17030439","article-title":"A comprehensive review of the neglected and emerging Oropouche virus","volume":"17","author":"Bai","year":"2025","journal-title":"Viruses"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0002","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s11908-025-00862-2","article-title":"Oropouche virus, A new emerging threat","volume":"27","author":"Labiod","year":"2025","journal-title":"Curr Infect Dis Rep"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0003","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1002\/ana.27139","article-title":"Oropouche Virus: an emerging neuroinvasive arbovirus","volume":"97","author":"Pastula","year":"2025","journal-title":"Annals of Neurology"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0004","doi-asserted-by":"crossref","DOI":"10.1186\/s40794-024-00236-x","article-title":"Addressing the emerging threat of Oropouche virus: implications and public health responses for healthcare systems","volume":"11","author":"Okesanya","year":"2025","journal-title":"Trop Dis Travel Med Vaccines"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0005","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0012962","article-title":"Clinical presentation of Oropouche virus infection: a systematic review and meta-analysis","volume":"19","author":"Wang","year":"2025","journal-title":"PLoS Negl Trop Dis"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0006","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s12941-016-0157-x","article-title":"ChikDenMaZika Syndrome: the challenge of diagnosing arboviral infections in the midst of concurrent epidemics","volume":"15","author":"Paniz-Mondolfi","year":"2016","journal-title":"Ann Clin Microbiol Antimicrob"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0007","doi-asserted-by":"crossref","first-page":"732","DOI":"10.4269\/ajtmh.2012.11-0485","article-title":"Short report: identification of Oropouche Orthobunyavirus in the cerebrospinal fluid of three patients in the Amazonas, Brazil","volume":"86","author":"Bastos M de","year":"2012","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0008","doi-asserted-by":"crossref","DOI":"10.1038\/s41541-022-00456-2","article-title":"Baseline mapping of Oropouche virology, epidemiology, therapeutics, and vaccine research and development","volume":"7","author":"Files","year":"2022","journal-title":"Npj Vaccines"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0009","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.3390\/v16091498","article-title":"Re-)emergence of Oropouche Virus (OROV) infections: systematic review and Meta-analysis of observational studies","volume":"16","author":"Ricc\u00f2","year":"2024","journal-title":"Viruses"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0010","doi-asserted-by":"crossref","DOI":"10.1099\/jgv.0.002027","article-title":"Oropouche Virus: an emerging orthobunyavirus","volume":"105","author":"Tilston-Lunel","year":"2024","journal-title":"J General Virol"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0011","unstructured":"2025-ago-13-actualizacion-epi-oropouche-eng n.d."},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0012","doi-asserted-by":"crossref","DOI":"10.3389\/ebm.2025.10647","article-title":"Insights into the expansion of Oropouche virus in Brazil: epidemiological and environmental aspects","volume":"250","author":"Godinho","year":"2025","journal-title":"Exp Biol Med"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0013","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.4269\/ajtmh.16-0672","article-title":"Oropouche virus: clinical, epidemiological, and molecular aspects of a neglected orthobunyavirus","volume":"96","author":"Travassos da Rosa","year":"2017","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0014","doi-asserted-by":"crossref","first-page":"3509","DOI":"10.1038\/s41591-024-03300-3","article-title":"Human outbreaks of a novel reassortant Oropouche virus in the Brazilian Amazon region","volume":"30","author":"Naveca","year":"2024","journal-title":"Nat Med"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0015","doi-asserted-by":"crossref","first-page":"353","DOI":"10.3390\/v17030353","article-title":"Oropouche Virus (OROV): expanding threats, shifting patterns, and the urgent need for collaborative research in Latin America","volume":"17","author":"Ribas Freitas","year":"2025","journal-title":"Viruses"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0016","article-title":"Re-emergence of Oropouche virus as a novel global threat","volume":"8","author":"Salvato","year":"2025","journal-title":"Current Res Micro Sci"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0017","doi-asserted-by":"crossref","first-page":"e439","DOI":"10.1016\/S1473-3099(23)00740-5","article-title":"Emergence of Oropouche fever in Latin America: a narrative review","volume":"24","author":"Wesselmann","year":"2024","journal-title":"Lancet Infect Diseases"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0018","doi-asserted-by":"crossref","DOI":"10.1016\/j.jviromet.2019.113685","article-title":"Development and analytical validation of a group-specific RT-qPCR assay for the detection of the Simbu serogroup orthobunyaviruses","volume":"271","author":"Camar\u00e3o","year":"2019","journal-title":"Journal of Virological Methods"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0019","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1590\/0074-02760150123","article-title":"Detection of Oropouche virus segment S in patients and inCulex quinquefasciatus in the state of Mato Grosso, Brazil","volume":"110","author":"Cardoso","year":"2015","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0020","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1099\/0022-1317-81-3-743","article-title":"Nucleotide sequences and phylogeny of the nucleocapsid gene of Oropouche virus - PubMed","volume":"81","author":"Saeed","year":"2000","journal-title":"J Gen Virol"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0021","doi-asserted-by":"crossref","DOI":"10.1098\/rsos.200636","article-title":"Presence of mismatches between diagnostic PCR assays and coronavirus SARS-CoV-2 genome","volume":"7","author":"Khan","year":"2020","journal-title":"R Soc Open Sci"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0022","doi-asserted-by":"crossref","first-page":"4968","DOI":"10.1128\/JCM.43.10.4968-4971.2005","article-title":"Sequence diversity in the glycoprotein B gene complicates real-time PCR assays for detection and quantification of cytomegalovirus","volume":"43","author":"Nye","year":"2005","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0023","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.gene.2007.09.013","article-title":"More effective purifying selection on RNA viruses than in DNA viruses","volume":"404","author":"Hughes","year":"2007","journal-title":"Gene"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0024","doi-asserted-by":"crossref","unstructured":"Cvijovic I, Good BH, Desai MM. The effect of strong purifying selection on genetic diversity. 209 2018;209:1235\u201378.","DOI":"10.1534\/genetics.118.301058"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0025","doi-asserted-by":"crossref","first-page":"643","DOI":"10.3390\/v11070643","article-title":"Effects of Arbovirus multi-host life cycles on dinucleotide and codon usage patterns","volume":"11","author":"Sexton","year":"2019","journal-title":"Viruses"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0026","doi-asserted-by":"crossref","first-page":"3299","DOI":"10.1128\/JCM.41.7.3299-3305.2003","article-title":"Rapid detection of Human pathogenic orthobunyaviruses","volume":"41","author":"Weidmann","year":"2003","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0027","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1590\/0074-02760160062","article-title":"Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses","volume":"112","author":"Naveca","year":"2017","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0028","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1093\/bib\/bbx108","article-title":"MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization","volume":"20","author":"Katoh","year":"2019","journal-title":"Briefings in Bioinf"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0029","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1093\/bioinformatics\/btp033","article-title":"Jalview Version 2\u2014A multiple sequence alignment editor and analysis workbench","volume":"25","author":"Waterhouse","year":"2009","journal-title":"Bioinformatics"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0030","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1093\/molbev\/msx335","article-title":"Datamonkey 2.0: a modern web application for characterizing selective and other evolutionary processes","volume":"35","author":"Weaver","year":"2018","journal-title":"Molecular Bio Evolut"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0031","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1093\/molbev\/msv022","article-title":"Less is more: an adaptive branch-site random effects model for efficient detection of episodic diversifying selection","volume":"32","author":"Smith","year":"2015","journal-title":"Molec Bio Evolut"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0032","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1002764","article-title":"Detecting individual sites subject to episodic diversifying selection","volume":"8","author":"Murrell","year":"2012","journal-title":"PLoS Genet"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0033","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1093\/molbev\/mst030","article-title":"FUBAR: a fast, unconstrained bayesian AppRoximation for inferring selection","volume":"30","author":"Murrell","year":"2013","journal-title":"Molec Bio Evolut"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0034","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0035","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera\u2014A visualization system for exploratory research and analysis","volume":"25","author":"Pettersen","year":"2004","journal-title":"J Comput Chem"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0036","series-title":"Virus as populations","first-page":"35","article-title":"Molecular Basis of genetic variation of viruses","author":"Domingo","year":"2016"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0037","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1038\/nrmicro.2016.46","article-title":"Reassortment in segmented RNA viruses: mechanisms and outcomes","volume":"14","author":"McDonald","year":"2016","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0038","doi-asserted-by":"crossref","first-page":"1660","DOI":"10.1128\/AEM.02403-07","article-title":"Quantification of the detrimental effect of a single primer-template mismatch by real-time PCR using the 16S rRNA gene as an example","volume":"74","author":"Bru","year":"2008","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0039","doi-asserted-by":"crossref","first-page":"109","DOI":"10.2353\/jmoldx.2010.090035","article-title":"The effect of primer-template mismatches on the detection and quantification of nucleic acids using the 5\u2019 nuclease assay","volume":"12","author":"Stadhouders","year":"2010","journal-title":"J Mol Diagn"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0040","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.mimet.2009.03.001","article-title":"Quantitative effects of position and type of single mismatch on single base primer extension","volume":"77","author":"Wu","year":"2009","journal-title":"J Microbio Methods"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0041","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.jcv.2005.02.010","article-title":"Sequence variation in primer targets affects the accuracy of viral quantitative PCR","volume":"34","author":"Whiley","year":"2005","journal-title":"J Clin Virol"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0042","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0007897","article-title":"Oropouche virus cases identified in Ecuador using an optimised qRT-PCR informed by metagenomic sequencing","volume":"14","author":"Wise","year":"2020","journal-title":"PLoS Negl Trop Dis"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0043","article-title":"Real-time RT-PCR for the detection and quantitation of Oropouche virus","volume":"96","author":"Rojas","year":"2020","journal-title":"Diagnos Microbio Infect Disease"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0044","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01127-19","article-title":"Evolutionary dynamics of Oropouche virus in South America","volume":"94","author":"Gutierrez","year":"2020","journal-title":"J Virol"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0045","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1007\/s00726-018-2560-4","article-title":"Nucleoprotein from the unique human infecting orthobunyavirus of Simbu serogroup (Oropouche virus) forms higher order oligomers in complex with nucleic acids in vitro","volume":"50","author":"Murillo","year":"2018","journal-title":"Amino Acids"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0046","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.01176-25","article-title":"Oropouche virus NSs protein suppresses host transcription by targeting the RNA polymerase II RPB1 protein","volume":"99","author":"Jurado-Cobena","year":"2025","journal-title":"J Virol"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0047","doi-asserted-by":"crossref","DOI":"10.1016\/j.nmni.2025.101596","article-title":"The emergence of oropouche fever: a potential new threat?","volume":"65","author":"Srivastava","year":"2025","journal-title":"New Microbes and New Infect"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0048","doi-asserted-by":"crossref","unstructured":"Yoshikawa R, Ishii Y, Sano N, Yasuda J. Nucleolar targeting by Oropouche virus nonstructural protein mediates RNA polymerase II degradation and interferon antagonism 2026;jiag158.","DOI":"10.1093\/infdis\/jiag158"},{"key":"10.1016\/j.diagmicrobio.2026.117559_bib0049","doi-asserted-by":"crossref","first-page":"703","DOI":"10.3390\/v14040703","article-title":"Global evolutionary history and dynamics of dengue viruses inferred from whole genome sequences","volume":"14","author":"Stica","year":"2022","journal-title":"Viruses"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889326003093?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889326003093?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:08:29Z","timestamp":1791241709000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889326003093"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":49,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["S0732889326003093"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2026.117559","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Selective pressure-driven identification of conserved regions for Oropouche virus molecular detection","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2026.117559","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"117559"}}