{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:50:11Z","timestamp":1792068611007,"version":"4.4.0"},"reference-count":17,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["CM24\/00250"],"award-info":[{"award-number":["CM24\/00250"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Microbiology and Infectious Disease"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.diagmicrobio.2026.117582","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T15:40:46Z","timestamp":1785253246000},"page":"117582","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Human versus machine: Assessing the Allplex\u2122 bacterial vaginosis plus assay (Seegene\u00ae) using Ison-Hay criteria and cut-off values interpretations in pregnant population"],"prefix":"10.1016","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1856-1725","authenticated-orcid":false,"given":"Alfredo","family":"Maldonado-Barrueco","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Ugarte-Merino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Fuensanta","family":"G\u00f3mez-Vera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Mart\u00edn-Boado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"de la Calle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanesa","family":"Gonz\u00e1lez-Montero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana I.","family":"Garc\u00eda-Palancar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juana","family":"Cacho-Calvo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inmaculada","family":"Quiles-Melero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.diagmicrobio.2026.117582_bib0001","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2021.672429","article-title":"Bacterial vaginosis: what do we currently know?","volume":"11","author":"Abou Chacra","year":"2022","journal-title":"Front Cell Infect Microbiol"},{"key":"10.1016\/j.diagmicrobio.2026.117582_bib0002","doi-asserted-by":"crossref","first-page":"6979","DOI":"10.3390\/ijerph110706979","article-title":"Anaerobes and bacterial vaginosis in pregnancy: virulence factors contributing to vaginal colonisation","volume":"11","author":"Africa","year":"2014","journal-title":"IJERPH"},{"key":"10.1016\/j.diagmicrobio.2026.117582_bib0003","doi-asserted-by":"crossref","first-page":"e2081","DOI":"10.1055\/s-0043-1770703","article-title":"Persistent bacterial vaginosis and risk for spontaneous preterm birth","volume":"41","author":"Blumenfeld","year":"2024","journal-title":"Am J Perinatol"},{"key":"10.1016\/j.diagmicrobio.2026.117582_bib0004","doi-asserted-by":"crossref","first-page":"2321","DOI":"10.1128\/JCM.00506-12","article-title":"Development and validation of a semiquantitative, multitarget PCR assay for diagnosis of bacterial vaginosis","volume":"50","author":"Cartwright","year":"2012","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2026.117582_bib0005","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/s13568-025-01847-z","article-title":"Best among the key molecular diagnostic markers of bacterial vaginosis","volume":"15","author":"Deng","year":"2025","journal-title":"AMB Expr"},{"key":"10.1016\/j.diagmicrobio.2026.117582_bib0006","doi-asserted-by":"crossref","DOI":"10.1016\/j.diagmicrobio.2019.114950","article-title":"An interpretation algorithm for molecular diagnosis of bacterial vaginosis in a maternity hospital using machine learning: proof-of-concept study","volume":"96","author":"Drew","year":"2020","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2026.117582_bib0007","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.3390\/biology11121797","article-title":"High-throughput sequencing-based analysis of changes in the vaginal microbiome during the disease course of patients with bacterial vaginosis: a case\u2013Control study","volume":"11","author":"Gao","year":"2022","journal-title":"Biology"},{"key":"10.1016\/j.diagmicrobio.2026.117582_bib0008","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1136\/sti.78.6.413","article-title":"Validation of a simplified grading of gram stained vaginal smears for use in genitourinary medicine clinics","volume":"78","author":"Ison","year":"2002","journal-title":"Sex Transm Infect"},{"key":"10.1016\/j.diagmicrobio.2026.117582_bib0009","doi-asserted-by":"crossref","DOI":"10.1016\/j.diagmicrobio.2025.117065","article-title":"Detection of bacterial vaginosis-associated bacteria and outcomes during clinical gynaecological conditions using qPCR: a retrospective cohort study","volume":"113","author":"Maldonado-Barrueco","year":"2025","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2026.117582_bib0010","doi-asserted-by":"crossref","DOI":"10.1016\/j.diagmicrobio.2024.116437","article-title":"Utility of culture and molecular methods using AllplexTM Bacterial Vaginosis Plus Assay (Seegene\u24c7) as a tool for endometriosis, infertility and recurrent pregnancy loss diagnosis","volume":"110","author":"Maldonado-Barrueco","year":"2024","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2026.117582_bib0011","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1086\/588661","article-title":"Molecular quantification of gardnerella vaginalis and atopobium vaginae loads to predict bacterial vaginosis","volume":"47","author":"Menard","year":"2008","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.diagmicrobio.2026.117582_bib0012","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1007\/s00404-022-06817-5","article-title":"Effect of bacterial vaginosis on preterm birth: a meta-analysis","volume":"308","author":"Mohanty","year":"2022","journal-title":"Arch Gynecol Obs"},{"key":"10.1016\/j.diagmicrobio.2026.117582_bib0013","doi-asserted-by":"crossref","DOI":"10.1128\/jcm.00837-22","article-title":"State of the Art for diagnosis of bacterial vaginosis","author":"Muzny","year":"2023","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.diagmicrobio.2026.117582_bib0014","unstructured":"Nugent RP, Krohn MA, Hillier SL. Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation. J Clin Microbiol n.d."},{"key":"10.1016\/j.diagmicrobio.2026.117582_bib0015","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.3855\/jidc.13091","article-title":"Quantification of Gardnerella vaginalis, atopobium vaginae and Lactobacillus spp. in bacterial vaginosis","volume":"15","author":"Numanovic","year":"2021","journal-title":"J Infect Dev Ctries"},{"key":"10.1016\/j.diagmicrobio.2026.117582_bib0016","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1136\/sextrans-2016-052883","article-title":"Assessment of atopobium vaginae and gardnerella vaginalis concentrations in a cohort of pregnant South African women","volume":"93","author":"Redelinghuys","year":"2017","journal-title":"Sex Transm Infect"},{"key":"10.1016\/j.diagmicrobio.2026.117582_bib0017","doi-asserted-by":"crossref","first-page":"1656","DOI":"10.1128\/AEM.02127-07","article-title":"Prevalence and abundance of uncultivated megasphaera -like bacteria in the Human vaginal environment","volume":"74","author":"Zozaya-Hinchliffe","year":"2008","journal-title":"Appl Env Microbiol"}],"container-title":["Diagnostic Microbiology and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889326003329?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0732889326003329?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:09:32Z","timestamp":1792066172000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0732889326003329"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["S0732889326003329"],"URL":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2026.117582","relation":{},"ISSN":["0732-8893"],"issn-type":[{"value":"0732-8893","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Human versus machine: Assessing the Allplex\u2122 bacterial vaginosis plus assay (Seegene\u00ae) using Ison-Hay criteria and cut-off values interpretations in pregnant population","name":"articletitle","label":"Article Title"},{"value":"Diagnostic Microbiology and Infectious Disease","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.diagmicrobio.2026.117582","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"117582"}}